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P26149

- 3BHS2_MOUSE

UniProt

P26149 - 3BHS2_MOUSE

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Protein

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2

Gene
Hsd3b2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.

Catalytic activityi

A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH.
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei155 – 1551Proton acceptor By similarity
Binding sitei159 – 1591NAD By similarity

GO - Molecular functioni

  1. 3-beta-hydroxy-delta5-steroid dehydrogenase activity Source: UniProtKB-EC
  2. steroid delta-isomerase activity Source: UniProtKB-EC

GO - Biological processi

  1. steroid biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi1.1.1.145. 244.
5.3.3.1. 244.
ReactomeiREACT_204887. Mineralocorticoid biosynthesis.
REACT_207160. Glucocorticoid biosynthesis.
REACT_220332. Androgen biosynthesis.
UniPathwayiUPA00062.

Names & Taxonomyi

Protein namesi
Recommended name:
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
Alternative name(s):
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II
Short name:
3-beta-HSD II
Including the following 2 domains:
3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC:1.1.1.145)
Alternative name(s):
3-beta-hydroxy-5-ene steroid dehydrogenase
Progesterone reductase
Steroid Delta-isomerase (EC:5.3.3.1)
Alternative name(s):
Delta-5-3-ketosteroid isomerase
Gene namesi
Name:Hsd3b2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:96234. Hsd3b2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei288 – 30821Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. mitochondrial membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3733733 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2PRO_0000087781Add
BLAST

Proteomic databases

PaxDbiP26149.
PRIDEiP26149.

PTM databases

PhosphoSiteiP26149.

Expressioni

Tissue specificityi

Liver and kidney.

Gene expression databases

BgeeiP26149.
GenevestigatoriP26149.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000102635.

Structurei

3D structure databases

ProteinModelPortaliP26149.
SMRiP26149. Positions 7-186.

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-beta-HSD family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0451.
GeneTreeiENSGT00550000074557.
HOGENOMiHOG000167989.
HOVERGENiHBG000014.
InParanoidiB1ARN7.
KOiK00070.
OMAiHEEECHE.
OrthoDBiEOG74J985.
TreeFamiTF343138.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01073. 3Beta_HSD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26149-1 [UniParc]FASTAAdd to Basket

« Hide

MPGWSCLVTG AGGFLGQRII QLLVQEEDLE EIRVLDKVFR PETRKEFFNL    50
ETSIKVTVLE GDILDTQYLR RACQGISVVI HTAAIIDVTG VIPRQTILDV 100
NLKGTQNLLE ACIQASVPAF IFSSSVDVAG PNSYKEIVLN GHEEECHEST 150
WSDPYPYSKK MAEKAVLAAN GSMLKNGGTL QTCALRPMCI YGERSPLISN 200
IIIMALKHKG ILRSFGKFNT ANPVYVGNVA WAHILAARGL RDPKKSPNIQ 250
GEFYYISDDT PHQSFDDISY TLSKEWGFCL DSSWSLPVPL LYWLAFLLET 300
VSFLLSPIYR YIPPFNRHLV TLSGSTFTFS YKKAQRDLGY EPLVSWEEAK 350
QKTSEWIGTL VEQHRETLDT KSQ 373
Length:373
Mass (Da):41,923
Last modified:June 28, 2011 - v4
Checksum:i2500AF16F38C081B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121G → K in AAH26757. 1 Publication
Sequence conflicti12 – 121G → K in AAH40397. 1 Publication
Sequence conflicti54 – 541I → N in AAH26757. 1 Publication
Sequence conflicti54 – 541I → N in AAH40397. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL606755 Genomic DNA. Translation: CAM13688.1.
BC026757 mRNA. Translation: AAH26757.1.
BC040397 mRNA. Translation: AAH40397.1.
M75886 mRNA. No translation available.
CCDSiCCDS17667.1.
RefSeqiNP_694873.2. NM_153193.3.
XP_006501101.1. XM_006501038.1.
UniGeneiMm.482364.

Genome annotation databases

EnsembliENSMUST00000107021; ENSMUSP00000102635; ENSMUSG00000063730.
ENSMUST00000107022; ENSMUSP00000102636; ENSMUSG00000063730.
ENSMUST00000177651; ENSMUSP00000136533; ENSMUSG00000063730.
GeneIDi15493.
KEGGimmu:15493.
UCSCiuc008qqc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL606755 Genomic DNA. Translation: CAM13688.1 .
BC026757 mRNA. Translation: AAH26757.1 .
BC040397 mRNA. Translation: AAH40397.1 .
M75886 mRNA. No translation available.
CCDSi CCDS17667.1.
RefSeqi NP_694873.2. NM_153193.3.
XP_006501101.1. XM_006501038.1.
UniGenei Mm.482364.

3D structure databases

ProteinModelPortali P26149.
SMRi P26149. Positions 7-186.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000102635.

PTM databases

PhosphoSitei P26149.

Proteomic databases

PaxDbi P26149.
PRIDEi P26149.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000107021 ; ENSMUSP00000102635 ; ENSMUSG00000063730 .
ENSMUST00000107022 ; ENSMUSP00000102636 ; ENSMUSG00000063730 .
ENSMUST00000177651 ; ENSMUSP00000136533 ; ENSMUSG00000063730 .
GeneIDi 15493.
KEGGi mmu:15493.
UCSCi uc008qqc.2. mouse.

Organism-specific databases

CTDi 3284.
MGIi MGI:96234. Hsd3b2.

Phylogenomic databases

eggNOGi COG0451.
GeneTreei ENSGT00550000074557.
HOGENOMi HOG000167989.
HOVERGENi HBG000014.
InParanoidi B1ARN7.
KOi K00070.
OMAi HEEECHE.
OrthoDBi EOG74J985.
TreeFami TF343138.

Enzyme and pathway databases

UniPathwayi UPA00062 .
BRENDAi 1.1.1.145. 244.
5.3.3.1. 244.
Reactomei REACT_204887. Mineralocorticoid biosynthesis.
REACT_207160. Glucocorticoid biosynthesis.
REACT_220332. Androgen biosynthesis.

Miscellaneous databases

NextBioi 288370.
PROi P26149.
SOURCEi Search...

Gene expression databases

Bgeei P26149.
Genevestigatori P26149.

Family and domain databases

Gene3Di 3.40.50.720. 2 hits.
InterProi IPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF01073. 3Beta_HSD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney and Liver.
  3. "Multiple forms of mouse 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase and differential expression in gonads, adrenal glands, liver, and kidneys of both sexes."
    Bain P.A., Yoo M., Clarke T., Hammond S.H., Payne A.H.
    Proc. Natl. Acad. Sci. U.S.A. 88:8870-8874(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 109-373.
    Strain: BALB/c.

Entry informationi

Entry namei3BHS2_MOUSE
AccessioniPrimary (citable) accession number: P26149
Secondary accession number(s): B1ARN7, Q8R0J6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: June 28, 2011
Last modified: September 3, 2014
This is version 123 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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