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P26149

- 3BHS2_MOUSE

UniProt

P26149 - 3BHS2_MOUSE

Protein

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2

Gene

Hsd3b2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 4 (28 Jun 2011)
      Previous versions | rss
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    Functioni

    3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.

    Catalytic activityi

    A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH.
    A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei155 – 1551Proton acceptorBy similarity
    Binding sitei159 – 1591NADBy similarity

    GO - Molecular functioni

    1. 3-beta-hydroxy-delta5-steroid dehydrogenase activity Source: UniProtKB-EC
    2. steroid delta-isomerase activity Source: UniProtKB-EC

    GO - Biological processi

    1. steroid biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase, Oxidoreductase

    Keywords - Biological processi

    Steroidogenesis

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BRENDAi1.1.1.145. 244.
    5.3.3.1. 244.
    ReactomeiREACT_204887. Mineralocorticoid biosynthesis.
    REACT_207160. Glucocorticoid biosynthesis.
    REACT_220332. Androgen biosynthesis.
    UniPathwayiUPA00062.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
    Alternative name(s):
    3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II
    Short name:
    3-beta-HSD II
    Including the following 2 domains:
    3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC:1.1.1.145)
    Alternative name(s):
    3-beta-hydroxy-5-ene steroid dehydrogenase
    Progesterone reductase
    Steroid Delta-isomerase (EC:5.3.3.1)
    Alternative name(s):
    Delta-5-3-ketosteroid isomerase
    Gene namesi
    Name:Hsd3b2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:96234. Hsd3b2.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. mitochondrial membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3733733 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2PRO_0000087781Add
    BLAST

    Proteomic databases

    PaxDbiP26149.
    PRIDEiP26149.

    PTM databases

    PhosphoSiteiP26149.

    Expressioni

    Tissue specificityi

    Liver and kidney.

    Gene expression databases

    BgeeiP26149.
    GenevestigatoriP26149.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000102635.

    Structurei

    3D structure databases

    ProteinModelPortaliP26149.
    SMRiP26149. Positions 7-186.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei288 – 30821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the 3-beta-HSD family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0451.
    GeneTreeiENSGT00550000074557.
    HOGENOMiHOG000167989.
    HOVERGENiHBG000014.
    InParanoidiB1ARN7.
    KOiK00070.
    OMAiHEEECHE.
    OrthoDBiEOG74J985.
    TreeFamiTF343138.

    Family and domain databases

    Gene3Di3.40.50.720. 2 hits.
    InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF01073. 3Beta_HSD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P26149-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPGWSCLVTG AGGFLGQRII QLLVQEEDLE EIRVLDKVFR PETRKEFFNL    50
    ETSIKVTVLE GDILDTQYLR RACQGISVVI HTAAIIDVTG VIPRQTILDV 100
    NLKGTQNLLE ACIQASVPAF IFSSSVDVAG PNSYKEIVLN GHEEECHEST 150
    WSDPYPYSKK MAEKAVLAAN GSMLKNGGTL QTCALRPMCI YGERSPLISN 200
    IIIMALKHKG ILRSFGKFNT ANPVYVGNVA WAHILAARGL RDPKKSPNIQ 250
    GEFYYISDDT PHQSFDDISY TLSKEWGFCL DSSWSLPVPL LYWLAFLLET 300
    VSFLLSPIYR YIPPFNRHLV TLSGSTFTFS YKKAQRDLGY EPLVSWEEAK 350
    QKTSEWIGTL VEQHRETLDT KSQ 373
    Length:373
    Mass (Da):41,923
    Last modified:June 28, 2011 - v4
    Checksum:i2500AF16F38C081B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti12 – 121G → K in AAH26757. (PubMed:15489334)Curated
    Sequence conflicti12 – 121G → K in AAH40397. (PubMed:15489334)Curated
    Sequence conflicti54 – 541I → N in AAH26757. (PubMed:15489334)Curated
    Sequence conflicti54 – 541I → N in AAH40397. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL606755 Genomic DNA. Translation: CAM13688.1.
    BC026757 mRNA. Translation: AAH26757.1.
    BC040397 mRNA. Translation: AAH40397.1.
    M75886 mRNA. No translation available.
    CCDSiCCDS17667.1.
    RefSeqiNP_694873.2. NM_153193.3.
    XP_006501101.1. XM_006501038.1.
    UniGeneiMm.482364.

    Genome annotation databases

    EnsembliENSMUST00000107021; ENSMUSP00000102635; ENSMUSG00000063730.
    ENSMUST00000107022; ENSMUSP00000102636; ENSMUSG00000063730.
    ENSMUST00000177651; ENSMUSP00000136533; ENSMUSG00000063730.
    GeneIDi15493.
    KEGGimmu:15493.
    UCSCiuc008qqc.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL606755 Genomic DNA. Translation: CAM13688.1 .
    BC026757 mRNA. Translation: AAH26757.1 .
    BC040397 mRNA. Translation: AAH40397.1 .
    M75886 mRNA. No translation available.
    CCDSi CCDS17667.1.
    RefSeqi NP_694873.2. NM_153193.3.
    XP_006501101.1. XM_006501038.1.
    UniGenei Mm.482364.

    3D structure databases

    ProteinModelPortali P26149.
    SMRi P26149. Positions 7-186.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000102635.

    PTM databases

    PhosphoSitei P26149.

    Proteomic databases

    PaxDbi P26149.
    PRIDEi P26149.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000107021 ; ENSMUSP00000102635 ; ENSMUSG00000063730 .
    ENSMUST00000107022 ; ENSMUSP00000102636 ; ENSMUSG00000063730 .
    ENSMUST00000177651 ; ENSMUSP00000136533 ; ENSMUSG00000063730 .
    GeneIDi 15493.
    KEGGi mmu:15493.
    UCSCi uc008qqc.2. mouse.

    Organism-specific databases

    CTDi 3284.
    MGIi MGI:96234. Hsd3b2.

    Phylogenomic databases

    eggNOGi COG0451.
    GeneTreei ENSGT00550000074557.
    HOGENOMi HOG000167989.
    HOVERGENi HBG000014.
    InParanoidi B1ARN7.
    KOi K00070.
    OMAi HEEECHE.
    OrthoDBi EOG74J985.
    TreeFami TF343138.

    Enzyme and pathway databases

    UniPathwayi UPA00062 .
    BRENDAi 1.1.1.145. 244.
    5.3.3.1. 244.
    Reactomei REACT_204887. Mineralocorticoid biosynthesis.
    REACT_207160. Glucocorticoid biosynthesis.
    REACT_220332. Androgen biosynthesis.

    Miscellaneous databases

    NextBioi 288370.
    PROi P26149.
    SOURCEi Search...

    Gene expression databases

    Bgeei P26149.
    Genevestigatori P26149.

    Family and domain databases

    Gene3Di 3.40.50.720. 2 hits.
    InterProi IPR002225. 3Beta_OHSteriod_DH/Estase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF01073. 3Beta_HSD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Kidney and Liver.
    3. "Multiple forms of mouse 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase and differential expression in gonads, adrenal glands, liver, and kidneys of both sexes."
      Bain P.A., Yoo M., Clarke T., Hammond S.H., Payne A.H.
      Proc. Natl. Acad. Sci. U.S.A. 88:8870-8874(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 109-373.
      Strain: BALB/c.

    Entry informationi

    Entry namei3BHS2_MOUSE
    AccessioniPrimary (citable) accession number: P26149
    Secondary accession number(s): B1ARN7, Q8R0J6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 124 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3