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P26149 (3BHS2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
Alternative name(s):
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II
Short name=3-beta-HSD II

Including the following 2 domains:

  1. 3-beta-hydroxy-Delta(5)-steroid dehydrogenase
    EC=1.1.1.145
    Alternative name(s):
    3-beta-hydroxy-5-ene steroid dehydrogenase
    Progesterone reductase
  2. Steroid Delta-isomerase
    EC=5.3.3.1
    Alternative name(s):
    Delta-5-3-ketosteroid isomerase
Gene names
Name:Hsd3b2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.

Catalytic activity

A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH.

A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.

Pathway

Lipid metabolism; steroid biosynthesis.

Subcellular location

Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein.

Tissue specificity

Liver and kidney.

Sequence similarities

Belongs to the 3-beta-HSD family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3733733 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
PRO_0000087781

Regions

Transmembrane288 – 30821Helical; Potential

Sites

Active site1551Proton acceptor By similarity
Binding site1591NAD By similarity

Experimental info

Sequence conflict121G → K in AAH26757. Ref.2
Sequence conflict121G → K in AAH40397. Ref.2
Sequence conflict541I → N in AAH26757. Ref.2
Sequence conflict541I → N in AAH40397. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P26149 [UniParc].

Last modified June 28, 2011. Version 4.
Checksum: 2500AF16F38C081B

FASTA37341,923
        10         20         30         40         50         60 
MPGWSCLVTG AGGFLGQRII QLLVQEEDLE EIRVLDKVFR PETRKEFFNL ETSIKVTVLE 

        70         80         90        100        110        120 
GDILDTQYLR RACQGISVVI HTAAIIDVTG VIPRQTILDV NLKGTQNLLE ACIQASVPAF 

       130        140        150        160        170        180 
IFSSSVDVAG PNSYKEIVLN GHEEECHEST WSDPYPYSKK MAEKAVLAAN GSMLKNGGTL 

       190        200        210        220        230        240 
QTCALRPMCI YGERSPLISN IIIMALKHKG ILRSFGKFNT ANPVYVGNVA WAHILAARGL 

       250        260        270        280        290        300 
RDPKKSPNIQ GEFYYISDDT PHQSFDDISY TLSKEWGFCL DSSWSLPVPL LYWLAFLLET 

       310        320        330        340        350        360 
VSFLLSPIYR YIPPFNRHLV TLSGSTFTFS YKKAQRDLGY EPLVSWEEAK QKTSEWIGTL 

       370 
VEQHRETLDT KSQ 

« Hide

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Kidney and Liver.
[3]"Multiple forms of mouse 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase and differential expression in gonads, adrenal glands, liver, and kidneys of both sexes."
Bain P.A., Yoo M., Clarke T., Hammond S.H., Payne A.H.
Proc. Natl. Acad. Sci. U.S.A. 88:8870-8874(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 109-373.
Strain: BALB/c.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL606755 Genomic DNA. Translation: CAM13688.1.
BC026757 mRNA. Translation: AAH26757.1.
BC040397 mRNA. Translation: AAH40397.1.
M75886 mRNA. No translation available.
CCDSCCDS17667.1.
RefSeqNP_694873.2. NM_153193.3.
XP_006501101.1. XM_006501038.1.
UniGeneMm.482364.

3D structure databases

ProteinModelPortalP26149.
SMRP26149. Positions 7-186.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000102635.

PTM databases

PhosphoSiteP26149.

Proteomic databases

PaxDbP26149.
PRIDEP26149.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000107021; ENSMUSP00000102635; ENSMUSG00000063730.
ENSMUST00000107022; ENSMUSP00000102636; ENSMUSG00000063730.
ENSMUST00000177651; ENSMUSP00000136533; ENSMUSG00000063730.
GeneID15493.
KEGGmmu:15493.
UCSCuc008qqc.2. mouse.

Organism-specific databases

CTD3284.
MGIMGI:96234. Hsd3b2.

Phylogenomic databases

eggNOGCOG0451.
GeneTreeENSGT00550000074557.
HOGENOMHOG000167989.
HOVERGENHBG000014.
InParanoidB1ARN7.
KOK00070.
OMAHEEECHE.
OrthoDBEOG74J985.
TreeFamTF343138.

Enzyme and pathway databases

BRENDA1.1.1.145. 244.
5.3.3.1. 244.
UniPathwayUPA00062.

Gene expression databases

BgeeP26149.
GenevestigatorP26149.

Family and domain databases

Gene3D3.40.50.720. 2 hits.
InterProIPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF01073. 3Beta_HSD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio288370.
PROP26149.
SOURCESearch...

Entry information

Entry name3BHS2_MOUSE
AccessionPrimary (citable) accession number: P26149
Secondary accession number(s): B1ARN7, Q8R0J6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: June 28, 2011
Last modified: July 9, 2014
This is version 122 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot