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Protein

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2

Gene

Hsd3b2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.

Catalytic activityi

A 3-beta-hydroxy-Delta(5)-steroid + NAD+ = a 3-oxo-Delta(5)-steroid + NADH.
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.

Pathway: steroid biosynthesis

This protein is involved in the pathway steroid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway steroid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei155 – 1551Proton acceptorBy similarity
Binding sitei159 – 1591NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiREACT_295803. Mineralocorticoid biosynthesis.
REACT_297756. Glucocorticoid biosynthesis.
REACT_305222. Androgen biosynthesis.
UniPathwayiUPA00062.

Names & Taxonomyi

Protein namesi
Recommended name:
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2
Alternative name(s):
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II
Short name:
3-beta-HSD II
Including the following 2 domains:
3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC:1.1.1.145)
Alternative name(s):
3-beta-hydroxy-5-ene steroid dehydrogenase
Progesterone reductase
Steroid Delta-isomerase (EC:5.3.3.1)
Alternative name(s):
Delta-5-3-ketosteroid isomerase
Gene namesi
Name:Hsd3b2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:96234. Hsd3b2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei288 – 30821HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 3733723 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2PRO_0000087781Add
BLAST

Proteomic databases

PaxDbiP26149.
PRIDEiP26149.

PTM databases

PhosphoSiteiP26149.

Expressioni

Tissue specificityi

Liver and kidney.

Gene expression databases

BgeeiP26149.
GenevisibleiP26149. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000102635.

Structurei

3D structure databases

ProteinModelPortaliP26149.
SMRiP26149. Positions 3-235.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 3-beta-HSD family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0451.
GeneTreeiENSGT00550000074557.
HOGENOMiHOG000167989.
HOVERGENiHBG000014.
InParanoidiP26149.
KOiK00070.
OMAiDTLYTCA.
OrthoDBiEOG74J985.
TreeFamiTF343138.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01073. 3Beta_HSD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26149-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGWSCLVTG AGGFLGQRII QLLVQEEDLE EIRVLDKVFR PETRKEFFNL
60 70 80 90 100
ETSIKVTVLE GDILDTQYLR RACQGISVVI HTAAIIDVTG VIPRQTILDV
110 120 130 140 150
NLKGTQNLLE ACIQASVPAF IFSSSVDVAG PNSYKEIVLN GHEEECHEST
160 170 180 190 200
WSDPYPYSKK MAEKAVLAAN GSMLKNGGTL QTCALRPMCI YGERSPLISN
210 220 230 240 250
IIIMALKHKG ILRSFGKFNT ANPVYVGNVA WAHILAARGL RDPKKSPNIQ
260 270 280 290 300
GEFYYISDDT PHQSFDDISY TLSKEWGFCL DSSWSLPVPL LYWLAFLLET
310 320 330 340 350
VSFLLSPIYR YIPPFNRHLV TLSGSTFTFS YKKAQRDLGY EPLVSWEEAK
360 370
QKTSEWIGTL VEQHRETLDT KSQ
Length:373
Mass (Da):41,923
Last modified:June 28, 2011 - v4
Checksum:i2500AF16F38C081B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121G → K in AAH26757 (PubMed:15489334).Curated
Sequence conflicti12 – 121G → K in AAH40397 (PubMed:15489334).Curated
Sequence conflicti54 – 541I → N in AAH26757 (PubMed:15489334).Curated
Sequence conflicti54 – 541I → N in AAH40397 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL606755 Genomic DNA. Translation: CAM13688.1.
BC026757 mRNA. Translation: AAH26757.1.
BC040397 mRNA. Translation: AAH40397.1.
M75886 mRNA. No translation available.
CCDSiCCDS17667.1.
RefSeqiNP_694873.2. NM_153193.3.
XP_006501101.1. XM_006501038.2.
UniGeneiMm.482364.

Genome annotation databases

EnsembliENSMUST00000107021; ENSMUSP00000102635; ENSMUSG00000063730.
ENSMUST00000107022; ENSMUSP00000102636; ENSMUSG00000063730.
ENSMUST00000177651; ENSMUSP00000136533; ENSMUSG00000063730.
GeneIDi15493.
KEGGimmu:15493.
UCSCiuc008qqc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL606755 Genomic DNA. Translation: CAM13688.1.
BC026757 mRNA. Translation: AAH26757.1.
BC040397 mRNA. Translation: AAH40397.1.
M75886 mRNA. No translation available.
CCDSiCCDS17667.1.
RefSeqiNP_694873.2. NM_153193.3.
XP_006501101.1. XM_006501038.2.
UniGeneiMm.482364.

3D structure databases

ProteinModelPortaliP26149.
SMRiP26149. Positions 3-235.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000102635.

PTM databases

PhosphoSiteiP26149.

Proteomic databases

PaxDbiP26149.
PRIDEiP26149.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000107021; ENSMUSP00000102635; ENSMUSG00000063730.
ENSMUST00000107022; ENSMUSP00000102636; ENSMUSG00000063730.
ENSMUST00000177651; ENSMUSP00000136533; ENSMUSG00000063730.
GeneIDi15493.
KEGGimmu:15493.
UCSCiuc008qqc.2. mouse.

Organism-specific databases

CTDi3284.
MGIiMGI:96234. Hsd3b2.

Phylogenomic databases

eggNOGiCOG0451.
GeneTreeiENSGT00550000074557.
HOGENOMiHOG000167989.
HOVERGENiHBG000014.
InParanoidiP26149.
KOiK00070.
OMAiDTLYTCA.
OrthoDBiEOG74J985.
TreeFamiTF343138.

Enzyme and pathway databases

UniPathwayiUPA00062.
ReactomeiREACT_295803. Mineralocorticoid biosynthesis.
REACT_297756. Glucocorticoid biosynthesis.
REACT_305222. Androgen biosynthesis.

Miscellaneous databases

NextBioi288370.
PROiP26149.
SOURCEiSearch...

Gene expression databases

BgeeiP26149.
GenevisibleiP26149. MM.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR002225. 3Beta_OHSteriod_DH/Estase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01073. 3Beta_HSD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney and Liver.
  3. "Multiple forms of mouse 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase and differential expression in gonads, adrenal glands, liver, and kidneys of both sexes."
    Bain P.A., Yoo M., Clarke T., Hammond S.H., Payne A.H.
    Proc. Natl. Acad. Sci. U.S.A. 88:8870-8874(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 109-373.
    Strain: BALB/c.

Entry informationi

Entry namei3BHS2_MOUSE
AccessioniPrimary (citable) accession number: P26149
Secondary accession number(s): B1ARN7, Q8R0J6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: June 28, 2011
Last modified: June 24, 2015
This is version 131 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.