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Protein

Polymerase basic protein 2

Gene

PB2

Organism
Influenza A virus (strain A/Swine/Tennessee/24/1977 H1N1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Plays an essential role in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. Recognizes and binds the 7-methylguanosine-containing cap of the target pre-RNA which is subsequently cleaved after 10-13 nucleotides by the viral protein PA. Plays a role in the initiation of the viral genome replication and modulates the activity of the ribonucleoprotein (RNP) complex. In addition, participates in the inhibition of type I interferon induction through interaction with and inhibition of the host mitochondrial antiviral signaling protein MAVS.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei627Mammalian adaptationUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCap snatching, Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host MAVS by virus, Inhibition of host RLR pathway by virus, Inhibition of host RNA polymerase II by virus, mRNA capping, mRNA processing, Viral immunoevasion, Viral transcription

Names & Taxonomyi

Protein namesi
Recommended name:
Polymerase basic protein 2UniRule annotation
Alternative name(s):
RNA-directed RNA polymerase subunit P3UniRule annotation
Gene namesi
Name:PB2UniRule annotation
OrganismiInfluenza A virus (strain A/Swine/Tennessee/24/1977 H1N1)
Taxonomic identifieri385606 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

  • Virion UniRule annotation
  • Host nucleus UniRule annotation
  • Host mitochondrion UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host mitochondrion, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000788431 – 759Polymerase basic protein 2Add BLAST759

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PB1 (via C-terminus). Interacts with nucleoprotein NP (via N-terminus). Interacts (via N-terminus) with host MAVS (via N-terminus); this interaction inhibits host innate immune response.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP26114.
SMRiP26114.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi736 – 739Nuclear localization signalUniRule annotation4

Sequence similaritiesi

Belongs to the influenza viruses PB2 family.UniRule annotation

Family and domain databases

HAMAPiMF_04062. INV_PB2. 1 hit.
InterProiView protein in InterPro
IPR037258. PDB2_C.
IPR001591. RNA_pol_PB2_orthomyxovir.
PfamiView protein in Pfam
PF00604. Flu_PB2. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001667. RNA_pol_PB2_orthomyxovir. 1 hit.
SUPFAMiSSF160453. SSF160453. 1 hit.

Sequencei

Sequence statusi: Complete.

P26114-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERIKELRNL MSQSRTRGIL TRTTVDHMAI IKKYTSGRQE KNPALRMKWM
60 70 80 90 100
MAMKYPITAD KRITETIPER NEQGQTLWSR TSDAGSDRVM VSPLAVTWWN
110 120 130 140 150
RNGPTASTIH YPKVYRTYFE KVERLKHGTF GPVHFRNQVK IRRRVDINPG
160 170 180 190 200
HADLSSKEAQ DVIMEVVFPN EVGARILTSE SQLMITKEKK EELQECKISP
210 220 230 240 250
LMVAYMLERE LVRKTRFLPV AGGTSSVYIE VLHLTQGACW EQLYTPGGEV
260 270 280 290 300
RNDDVDQSLI IAARSIVRRA TVSADPLASL LEMCHSTQIG GVRMVDILKQ
310 320 330 340 350
NPTEEQAVDI CKAAMGLRIS SSFSFGGFTF KRTSGSSTKR EEEVLTGNLQ
360 370 380 390 400
TLKIRVHEGY EEFTMVGKKA TAILRKATRR LVQLIVSGRD EQSIAEAIIV
410 420 430 440 450
AMVFSQEDCV IKAVRGDLNF VNRANQRLNP MHQLLRHFQK DAKILFQNWG
460 470 480 490 500
IESIDNVMGM IGILPDLTPS TEKSMRGIRI SKMGVDEYSS TERVVVSIDR
510 520 530 540 550
FLRVRDQQGN VLLSPEEVSE TQGTEKLTIT YSSSMMWEVN GPESVLVNTY
560 570 580 590 600
QWIIRNWETV KIQWSQDPTM LYNKMEFEPF QSLVPKAARG QYSGFVRTLF
610 620 630 640 650
QQMRDVLGTF DTVQIIKLLP FAAAPPKQSR MQFSSLTVNV RGSGMRILIR
660 670 680 690 700
GNSPVFNYNK STKRLTVLGK DAGALNEDPD EGTAGVESAV LRGFLILGRE
710 720 730 740 750
DRRYGPALSI NQLSSLAKGE KANVLIGQGD VVLVMKRKRD SSILTDSQTA

TKRIRMAIN
Length:759
Mass (Da):85,732
Last modified:May 1, 1992 - v1
Checksum:iCC0B1677BC5FB80B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73513 Genomic RNA. Translation: AAA43125.1.

Similar proteinsi

Entry informationi

Entry nameiPB2_I77AC
AccessioniPrimary (citable) accession number: P26114
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: November 22, 2017
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families