PB2

Functionⁱ

Plays an essential role in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. Recognizes and binds the 7-methylguanosine-containing cap of the target pre-RNA which is subsequently cleaved after 10-13 nucleotides by the viral protein PA. Plays a role in the initiation of the viral genome replication and modulates the activity of the ribonucleoprotein (RNP) complex. In addition, participates in the inhibition of type I interferon induction through interaction with and inhibition of the host mitochondrial antiviral signaling protein MAVS.UniRule annotation

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	627	Mammalian adaptationUniRule annotation		1

GO - Molecular functionⁱ

RNA binding Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

7-methylguanosine mRNA capping Source: UniProtKB-KW
cap snatching Source: UniProtKB-KW
suppression by virus of host MAVS activity Source: UniProtKB-KW
suppression by virus of host RNA polymerase II activity Source: UniProtKB-KW
transcription, DNA-templated Source: InterPro
viral RNA genome replication Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Cap snatching, Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host MAVS by virus, Inhibition of host RLR pathway by virus, Inhibition of host RNA polymerase II by virus, mRNA capping, mRNA processing, Viral immunoevasion, Viral transcription

Biological process

Cap snatching, Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host MAVS by virus, Inhibition of host RLR pathway by virus, Inhibition of host RNA polymerase II by virus, mRNA capping, mRNA processing, Viral immunoevasion, Viral transcription

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Polymerase basic protein 2UniRule annotation Alternative name(s): RNA-directed RNA polymerase subunit P3UniRule annotation
Gene namesⁱ	Name:PB2UniRule annotation
Organismⁱ	Influenza A virus (strain A/Swine/Tennessee/24/1977 H1N1)
Taxonomic identifierⁱ	385606 [NCBI]
Taxonomic lineageⁱ	Viruses › ssRNA viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus A › Influenza A virus › H1N1 subtype
Virus hostⁱ	Aves [TaxID: 8782] Homo sapiens (Human) [TaxID: 9606] Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationⁱ

GO - Cellular componentⁱ

host cell mitochondrion Source: UniProtKB-SubCell
host cell nucleus Source: UniProtKB-SubCell
virion Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Host mitochondrion, Host nucleus, Virion

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000078843	1 – 759	Polymerase basic protein 2Add BLAST		759

Interactionⁱ

Subunit structureⁱ

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PB1 (via C-terminus). Interacts with nucleoprotein NP (via N-terminus). Interacts (via N-terminus) with host MAVS (via N-terminus); this interaction inhibits host innate immune response.UniRule annotation

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P26114.
SMRⁱ	P26114.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	736 – 739	Nuclear localization signalUniRule annotation		4

Sequence similaritiesⁱ

Belongs to the influenza viruses PB2 family.UniRule annotation

Family and domain databases

Gene3Dⁱ	3.30.30.90. 1 hit.
HAMAPⁱ	MF_04062. INV_PB2. 1 hit.
InterProⁱ	View protein in InterPro IPR001591. INV_PB2. IPR037258. PDB2_C.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00604. Flu_PB2. 1 hit.
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD001667. RNA_pol_PB2_orthomyxovir. 1 hit.
SUPFAMⁱ	SSF160453. SSF160453. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

P26114-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MERIKELRNL MSQSRTRGIL TRTTVDHMAI IKKYTSGRQE KNPALRMKWM 
        60         70         80         90        100
MAMKYPITAD KRITETIPER NEQGQTLWSR TSDAGSDRVM VSPLAVTWWN 
       110        120        130        140        150
RNGPTASTIH YPKVYRTYFE KVERLKHGTF GPVHFRNQVK IRRRVDINPG 
       160        170        180        190        200
HADLSSKEAQ DVIMEVVFPN EVGARILTSE SQLMITKEKK EELQECKISP 
       210        220        230        240        250
LMVAYMLERE LVRKTRFLPV AGGTSSVYIE VLHLTQGACW EQLYTPGGEV 
       260        270        280        290        300
RNDDVDQSLI IAARSIVRRA TVSADPLASL LEMCHSTQIG GVRMVDILKQ 
       310        320        330        340        350
NPTEEQAVDI CKAAMGLRIS SSFSFGGFTF KRTSGSSTKR EEEVLTGNLQ 
       360        370        380        390        400
TLKIRVHEGY EEFTMVGKKA TAILRKATRR LVQLIVSGRD EQSIAEAIIV 
       410        420        430        440        450
AMVFSQEDCV IKAVRGDLNF VNRANQRLNP MHQLLRHFQK DAKILFQNWG 
       460        470        480        490        500
IESIDNVMGM IGILPDLTPS TEKSMRGIRI SKMGVDEYSS TERVVVSIDR 
       510        520        530        540        550
FLRVRDQQGN VLLSPEEVSE TQGTEKLTIT YSSSMMWEVN GPESVLVNTY 
       560        570        580        590        600
QWIIRNWETV KIQWSQDPTM LYNKMEFEPF QSLVPKAARG QYSGFVRTLF 
       610        620        630        640        650
QQMRDVLGTF DTVQIIKLLP FAAAPPKQSR MQFSSLTVNV RGSGMRILIR 
       660        670        680        690        700
GNSPVFNYNK STKRLTVLGK DAGALNEDPD EGTAGVESAV LRGFLILGRE 
       710        720        730        740        750
DRRYGPALSI NQLSSLAKGE KANVLIGQGD VVLVMKRKRD SSILTDSQTA 

TKRIRMAIN

Length:759

Mass (Da):85,732

Last modified:May 1, 1992 - v1

Checksum:ⁱCC0B1677BC5FB80B

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M73513 Genomic RNA. Translation: AAA43125.1.

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

M73513 Genomic RNA. Translation: AAA43125.1.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P26114	P03427 Q6VE62 L8B2Z8 M9YXQ7 Q5S6W2 V5R4A4 Q6J864 Q6DNL7 I7HH08 F1CTB3 +47569	Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1)) Influenza A virus (A/chicken/Netherlands/1/03(H7N7)) Influenza A virus (A/duck/Vietnam/OIE-3241/2012(H3N8)) Influenza A virus (A/great black-backed gull/Newfoundland/MW174/2010(H1)) Influenza A virus (A/chicken/Korea/SNU0037/00(H9N2)) Influenza A virus (A/swine/North Carolina/SG1393/2011) Influenza A virus (A/G.H/HK/793.1/02 (H5N1)) Influenza A virus (A/Ck/HK/3176.3/2002(H5N1)) Influenza A virus (A/duck/Vietnam/OIE-1234/2012(H3N2)) Influenza A virus (A/herring gull/Atyrau/2186/2007(H11N2)) And more	759	UniRef90_P03427	Cluster: Polymerase basic protein 2	47,580

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P26114	P03427 Q6VE62 L8B2Z8 M9YXQ7 Q5S6W2 V5R4A4 Q6J864 Q6DNL7 I7HH08 F1CTB3 +47577	Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1)) Influenza A virus (A/chicken/Netherlands/1/03(H7N7)) Influenza A virus (A/duck/Vietnam/OIE-3241/2012(H3N8)) Influenza A virus (A/great black-backed gull/Newfoundland/MW174/2010(H1)) Influenza A virus (A/chicken/Korea/SNU0037/00(H9N2)) Influenza A virus (A/swine/North Carolina/SG1393/2011) Influenza A virus (A/G.H/HK/793.1/02 (H5N1)) Influenza A virus (A/Ck/HK/3176.3/2002(H5N1)) Influenza A virus (A/duck/Vietnam/OIE-1234/2012(H3N2)) Influenza A virus (A/herring gull/Atyrau/2186/2007(H11N2)) And more	759	UniRef50_P03427	Cluster: Polymerase basic protein 2	47,588

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M73513 Genomic RNA. Translation: AAA43125.1.

Select the link destinations:
EMBLⁱ
GenBankⁱ
DDBJⁱ

Links Updated

M73513 Genomic RNA. Translation: AAA43125.1.

3D structure databases

ProteinModelPortalⁱ	P26114.
SMRⁱ	P26114.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Family and domain databases

Gene3Dⁱ	3.30.30.90. 1 hit.
HAMAPⁱ	MF_04062. INV_PB2. 1 hit.
InterProⁱ	View protein in InterPro IPR001591. INV_PB2. IPR037258. PDB2_C.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00604. Flu_PB2. 1 hit.
ProDomⁱ	View protein in ProDom or Entries sharing at least one domain PD001667. RNA_pol_PB2_orthomyxovir. 1 hit.
SUPFAMⁱ	SSF160453. SSF160453. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PB2_I77AC
Accessionⁱ	P26114Primary (citable) accession number: P26114
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
	Last sequence update:	May 1, 1992
	Last modified:	March 28, 2018
	This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Viral Protein Annotation Program

UniProtKB - P26114 (PB2_I77AC)

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Polymerase basic protein 2

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Functionⁱ

Sites

GO - Molecular functionⁱ

GO - Biological processⁱ

Keywordsⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Molecule processing

Interactionⁱ

Subunit structureⁱ

Structureⁱ

3D structure databases

Family & Domainsⁱ

Motif

Sequence similaritiesⁱ

Family and domain databases

Sequenceⁱ

Sequence databases

Similar proteinsⁱ

Cross-referencesⁱ

Sequence databases

3D structure databases

Protocols and materials databases

Family and domain databases

Entry informationⁱ

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P26114 (PB2_I77AC)

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Polymerase basic protein 2

PB2

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Family and domain databases

Sequence databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ