P26114 (PB2_I77AC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polymerase basic protein 2

Alternative name(s):
RNA-directed RNA polymerase subunit P3

Gene names

Name:

PB2

Organism

Influenza A virus (strain A/Swine/Tennessee/24/1977 H1N1)

Taxonomic identifier

385606 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus A › Influenza A virus › H1N1 subtype

Virus host

Aves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length	759 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. Binds the cap of the target pre-RNA which is subsequently cleaved by PB1. May play a role in genome replication By similarity.
Subunit structure	Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.
Subcellular location	Virion. Host nucleus. Host mitochondrion By similarity.
Sequence similarities	Belongs to the influenza viruses PB2 family.

Ontologies

Keywords
Biological process	Cap snatching Host gene expression shutoff by virus Host transcription shutoff by virus Host-virus interaction Inhibition of host RNA polymerase II by virus mRNA capping mRNA processing
Cellular component	Host mitochondrion Host nucleus Virion
Gene Ontology (GO)
Biological_process	7-methylguanosine mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW cap snatching Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host RNA polymerase II activity Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-templated Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 759	759	Polymerase basic protein 2		PRO_0000078843
Regions
	Motif	736 – 739	4	Nuclear localization signal By similarity

Sequences

	Sequence		Length	Mass (Da)
	P26114 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: CC0B1677BC5FB80B	FASTA	759	85,732
	10 20 30 40 50 60 MERIKELRNL MSQSRTRGIL TRTTVDHMAI IKKYTSGRQE KNPALRMKWM MAMKYPITAD 70 80 90 100 110 120 KRITETIPER NEQGQTLWSR TSDAGSDRVM VSPLAVTWWN RNGPTASTIH YPKVYRTYFE 130 140 150 160 170 180 KVERLKHGTF GPVHFRNQVK IRRRVDINPG HADLSSKEAQ DVIMEVVFPN EVGARILTSE 190 200 210 220 230 240 SQLMITKEKK EELQECKISP LMVAYMLERE LVRKTRFLPV AGGTSSVYIE VLHLTQGACW 250 260 270 280 290 300 EQLYTPGGEV RNDDVDQSLI IAARSIVRRA TVSADPLASL LEMCHSTQIG GVRMVDILKQ 310 320 330 340 350 360 NPTEEQAVDI CKAAMGLRIS SSFSFGGFTF KRTSGSSTKR EEEVLTGNLQ TLKIRVHEGY 370 380 390 400 410 420 EEFTMVGKKA TAILRKATRR LVQLIVSGRD EQSIAEAIIV AMVFSQEDCV IKAVRGDLNF 430 440 450 460 470 480 VNRANQRLNP MHQLLRHFQK DAKILFQNWG IESIDNVMGM IGILPDLTPS TEKSMRGIRI 490 500 510 520 530 540 SKMGVDEYSS TERVVVSIDR FLRVRDQQGN VLLSPEEVSE TQGTEKLTIT YSSSMMWEVN 550 560 570 580 590 600 GPESVLVNTY QWIIRNWETV KIQWSQDPTM LYNKMEFEPF QSLVPKAARG QYSGFVRTLF 610 620 630 640 650 660 QQMRDVLGTF DTVQIIKLLP FAAAPPKQSR MQFSSLTVNV RGSGMRILIR GNSPVFNYNK 670 680 690 700 710 720 STKRLTVLGK DAGALNEDPD EGTAGVESAV LRGFLILGRE DRRYGPALSI NQLSSLAKGE 730 740 750 KANVLIGQGD VVLVMKRKRD SSILTDSQTA TKRIRMAIN

References

[1]

"Evolution of influenza A virus PB2 genes: implications for evolution of the ribonucleoprotein complex and origin of human influenza A virus." Gorman O.T., Donis R.O., Kawaoka Y., Webster R.G. J. Virol. 64:4893-4902(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M73513 Genomic RNA. Translation: AAA43125.1.
3D structure databases
ProteinModelPortal	P26114.
SMR	P26114. Positions 319-483, 685-759.
ModBase	Search...
MobiDB	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001591. RNA_pol_PB2_orthomyxovir. [Graphical view]
Pfam	PF00604. Flu_PB2. 1 hit. [Graphical view]
ProDom	PD001667. RNA_pol_PB2_orthomyxovir. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

PB2_I77AC

Accession

Primary (citable) accession number: P26114

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	February 19, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program