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P26091

- NCAP_I82A4

UniProt

P26091 - NCAP_I82A4

Protein

Nucleoprotein

Gene

NP

Organism
Influenza A virus (strain A/Swine/Hong Kong/126/1982 H3N2)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus By similarity.By similarity

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW
    2. structural molecule activity Source: InterPro

    GO - Biological processi

    1. viral entry into host cell Source: UniProtKB-KW
    2. viral penetration into host nucleus Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Biological processi

    Host-virus interaction, Viral penetration into host nucleus, Virus entry into host cell

    Keywords - Ligandi

    RNA-binding, Viral nucleoprotein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nucleoprotein
    Alternative name(s):
    Nucleocapsid protein
    Short name:
    Protein N
    Gene namesi
    Name:NP
    OrganismiInfluenza A virus (strain A/Swine/Hong Kong/126/1982 H3N2)
    Taxonomic identifieri382848 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
    Virus hostiAves [TaxID: 8782]
    Cetacea (whales) [TaxID: 9721]
    Homo sapiens (Human) [TaxID: 9606]
    Phocidae (true seals) [TaxID: 9709]
    Sus scrofa (Pig) [TaxID: 9823]

    Subcellular locationi

    GO - Cellular componenti

    1. helical viral capsid Source: UniProtKB-KW
    2. host cell nucleus Source: UniProtKB-SubCell
    3. ribonucleoprotein complex Source: UniProtKB-KW
    4. viral nucleocapsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Helical capsid protein, Host nucleus, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 498498NucleoproteinPRO_0000079121Add
    BLAST

    Post-translational modificationi

    Late in virus-infected cells, may be cleaved from a 56-kDa protein to a 53-kDa protein by a cellular caspase. This cleavage might be a marker for the onset of apoptosis in infected cells or have a specific function in virus host interaction By similarity.By similarity

    Interactioni

    Subunit structurei

    Homomultimerizes to form the nucleocapsid. May bind human exportin-1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases By similarity.By similarity

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1HOCX-ray2.40C366-374[»]
    ProteinModelPortaliP26091.
    SMRiP26091. Positions 22-496.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP26091.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1 – 1818Unconventional nuclear localization signalBy similarityAdd
    BLAST
    Motifi198 – 21619Bipartite nuclear localization signalBy similarityAdd
    BLAST

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR002141. Flu_NP.
    [Graphical view]
    PfamiPF00506. Flu_NP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P26091-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASQGTKRSY EQMETGGERQ NATEIRASVG RMVGGIGRFY IQMCTELKLS    50
    DYEGRLIQNS ITIERMVLSA FDERRNKYLE EHPSAGKDPK KTGGPIYRRR 100
    DGKWMRELIL YDKEEIRRIW RQANNGEDAT AGLTHLMIWH SNLNDATYQR 150
    TRALVRTGMD PRMCSLMQGS TLPRRSGAAG AAVKGVGTMV MELIRMVKRG 200
    INDRNFWRGE NGRRTRIAYE RMCNILKGKF QTAAQRAMMD QVRESRNPGN 250
    AEIEDLIFLA RSALILRGSV AHKSCLPACV YGLAVASGYD FEREGYSLVG 300
    IDPFRLLQNS QVLSLIRPNE NPAHKSQLVW MACHSAAFED LRVSSFIRGT 350
    RVIPRGQLST RGVQIASNEN METMDSSTLE LRSRYWAIRT RSGGNTNQQR 400
    ASAGQISVQP TFSVQRNLPF ERATIMAAFT GNTEGRTSDM RTEIIRMMES 450
    ARPEDVSFQG RGVFELSDEK ATNPIVPSFD MSNEGSYFFG DNAEEYDN 498
    Length:498
    Mass (Da):56,250
    Last modified:May 1, 1992 - v1
    Checksum:i30EFB084528BFD06
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63771 Genomic RNA. Translation: AAA52270.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63771 Genomic RNA. Translation: AAA52270.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1HOC X-ray 2.40 C 366-374 [» ]
    ProteinModelPortali P26091.
    SMRi P26091. Positions 22-496.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P26091.

    Family and domain databases

    InterProi IPR002141. Flu_NP.
    [Graphical view ]
    Pfami PF00506. Flu_NP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Evolution of influenza A virus nucleoprotein genes: implications for the origins of H1N1 human and classical swine viruses."
      Gorman O.T., Bean W.J., Kawaoka Y., Donatelli I., Guo Y., Webster R.G.
      J. Virol. 65:3704-3714(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiNCAP_I82A4
    AccessioniPrimary (citable) accession number: P26091
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3