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Protein

Nucleoprotein

Gene

NP

Organism
Influenza A virus (strain A/Swine/Iowa/1946 H1N1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Host-virus interaction, Viral penetration into host nucleus, Virus entry into host cell

Keywords - Ligandi

RNA-binding, Viral nucleoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoprotein
Alternative name(s):
Nucleocapsid protein
Short name:
Protein N
Gene namesi
Name:NP
OrganismiInfluenza A virus (strain A/Swine/Iowa/1946 H1N1)
Taxonomic identifieri383537 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Helical capsid protein, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 498498NucleoproteinPRO_0000079125Add
BLAST

Post-translational modificationi

Late in virus-infected cells, may be cleaved from a 56-kDa protein to a 53-kDa protein by a cellular caspase. This cleavage might be a marker for the onset of apoptosis in infected cells or have a specific function in virus host interaction (By similarity).By similarity

Interactioni

Subunit structurei

Homomultimerizes to form the nucleocapsid. May bind human exportin-1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP26079.
SMRiP26079. Positions 23-496.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1 – 1818Unconventional nuclear localization signalBy similarityAdd
BLAST
Motifi198 – 21619Bipartite nuclear localization signalBy similarityAdd
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR002141. Flu_NP.
[Graphical view]
PfamiPF00506. Flu_NP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P26079-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASQGTKRSY EQMETGGERQ NTTEIRASVG RMIGGIGRFY IQMCTELKLS
60 70 80 90 100
DYEGRLIQNS ITIERMVLSA FDERRNKYLE EHPSAGKDPK RTGGPIYRRI
110 120 130 140 150
DGKWIRELIL YDKEEISRIW RQANNGEDAT AGLTHMMIWH SNLNDATYQR
160 170 180 190 200
TRALVRTGMD PRMCSLMQGS TLPRRSGAAG AAVKGVGTMV MELIRMIKRG
210 220 230 240 250
INDRNFWRGE NGRRTRIAYE RMCNILKGKF QTAAQKAMMD QVRESRNPGN
260 270 280 290 300
AEIEDLIFLA RSALILRGSV AHKSCLPACV YGLAVASGHD FEREGYSLVG
310 320 330 340 350
IDPFRLLQNS QVFSLIRPNE NPAHKSQLVW MACHSAAFED LRVSSFIRGK
360 370 380 390 400
RVVPRGQLST RGVQIASNEN METMDSSTLE LRSRYWAIRT RSGGNTNQQR
410 420 430 440 450
ASAGQISVQP TFSVQRNLPF ERATVMAAFT GNTEGRTSDM RTEIIRIMES
460 470 480 490
ARPEDVSFQG RGVFELSDEK ATSPIVPSFD MSNEGSYFFG DNAEEYDN
Length:498
Mass (Da):56,158
Last modified:May 1, 1992 - v1
Checksum:i1C8731971D8D96F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63759 Genomic RNA. Translation: AAA52258.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63759 Genomic RNA. Translation: AAA52258.1.

3D structure databases

ProteinModelPortaliP26079.
SMRiP26079. Positions 23-496.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR002141. Flu_NP.
[Graphical view]
PfamiPF00506. Flu_NP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Evolution of influenza A virus nucleoprotein genes: implications for the origins of H1N1 human and classical swine viruses."
    Gorman O.T., Bean W.J., Kawaoka Y., Donatelli I., Guo Y., Webster R.G.
    J. Virol. 65:3704-3714(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiNCAP_I46A1
AccessioniPrimary (citable) accession number: P26079
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 24, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.