Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nucleoprotein

Gene

NP

Organism
Influenza A virus (strain A/New Jersey/8/1976 H1N1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding, Viral nucleoprotein
Biological processHost-virus interaction, Viral penetration into host nucleus, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
NucleoproteinUniRule annotation
Alternative name(s):
Nucleocapsid proteinUniRule annotation
Short name:
Protein NUniRule annotation
Gene namesi
Name:NPUniRule annotation
OrganismiInfluenza A virus (strain A/New Jersey/8/1976 H1N1)
Taxonomic identifieri379756 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

P26074:
  • Virion UniRule annotation
  • Host nucleus UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, Helical capsid protein, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000790881 – 498NucleoproteinAdd BLAST498

Post-translational modificationi

Late in virus-infected cells, may be cleaved from a 56-kDa protein to a 53-kDa protein by a cellular caspase. This cleavage might be a marker for the onset of apoptosis in infected cells or have a specific function in virus host interaction.UniRule annotation

Interactioni

Subunit structurei

Homomultimerizes to form the nucleocapsid. May bind host exportin-1/XPO1. Binds to viral genomic RNA. Protein-RNA contacts are mediated by a combination of electrostatic interactions between positively charged residues and the phosphate backbone and planar interactions between aromatic side chains and bases.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP26074.
SMRiP26074.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1 – 18Unconventional nuclear localization signalUniRule annotationAdd BLAST18
Motifi198 – 216Bipartite nuclear localization signalUniRule annotationAdd BLAST19

Sequence similaritiesi

Belongs to the influenza viruses nucleoprotein family.UniRule annotation

Family and domain databases

HAMAPiMF_04070. INFV_NCAP. 1 hit.
InterProiView protein in InterPro
IPR002141. Flu_NP.
PfamiView protein in Pfam
PF00506. Flu_NP. 1 hit.

Sequencei

Sequence statusi: Complete.

P26074-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASQGTKRSY EQMETGGERQ DATEIRASVG RMIGGIGRFY IQMCTELKLS
60 70 80 90 100
DYEGRLIQNS ITIERMVLSA FDERRNKYLE EHPSAGKDPK KTGGPIYKRI
110 120 130 140 150
DGKWMRELIL YDKEEIRRVW RQANNGEDAT AGLTHIMIWH SNLNDATYQR
160 170 180 190 200
TRALVRTGMD PRMCSLMQGS TLPRRSGAAG AAVKGVGTIA MELIGMIKRG
210 220 230 240 250
INDRNFWRGE NGRRTRIAYE RMCNILKGKF QTAAQRAMMD QVRESRNPGN
260 270 280 290 300
AEIEDLIFLA RSALILRGSV AHKSCLPACV YGLAVASGHD FEREGYSLVG
310 320 330 340 350
IDPFKLLQNS QVFSLIRPNE NPAHKSQLVW MACHSAAFED LRVSGFIRGK
360 370 380 390 400
KVVPRGRLST RGVQIASNEN VEAMDSSTLE LRSRYWAIRT RSGGNTNQQK
410 420 430 440 450
ASADQISVQP TFSVQRNLPF ERATVMAAFI GDNEGRTSDM RTEIIRMMES
460 470 480 490
AKPEDLSFQG RGVFELSDEK ATNPIVPSFD MNNEGSYFFG DNAEEYDN
Length:498
Mass (Da):56,005
Last modified:May 1, 1992 - v1
Checksum:i34AB4786570E78EB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti195G → R in AAA73108 (PubMed:1536092).1
Sequence conflicti404D → G in AAA73108 (PubMed:1536092).1
Sequence conflicti483N → Y in AAA73108 (PubMed:1536092).1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63754 Genomic RNA. Translation: AAA52253.1.
M76606 mRNA. Translation: AAA73108.1.

Similar proteinsi

Entry informationi

Entry nameiNCAP_I76A7
AccessioniPrimary (citable) accession number: P26074
Secondary accession number(s): Q67225
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: October 25, 2017
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families