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Protein

CD44 antigen

Gene

Cd44

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Receptor for LGALS9; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei44HyaluronanBy similarity1
Binding sitei81HyaluronanBy similarity1
Binding sitei82HyaluronanBy similarity1
Binding sitei108HyaluronanBy similarity1

GO - Molecular functioni

  • epidermal growth factor receptor binding Source: RGD
  • hyaluronic acid binding Source: RGD
  • phosphoprotein binding Source: RGD
  • protein kinase binding Source: RGD

GO - Biological processi

  • blood vessel maturation Source: RGD
  • cell adhesion Source: RGD
  • cell migration Source: RGD
  • inflammatory response Source: RGD
  • macrophage fusion Source: RGD
  • neuron projection development Source: RGD
  • positive regulation of neutrophil apoptotic process Source: RGD
  • regulation of cell growth Source: RGD
  • regulation of lamellipodium morphogenesis Source: UniProtKB
  • response to organic cyclic compound Source: RGD
  • response to vitamin A Source: RGD
  • wound healing, spreading of cells Source: UniProtKB

Keywordsi

Molecular functionReceptor
Biological processCell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
CD44 antigen
Alternative name(s):
Extracellular matrix receptor III
Short name:
ECMR-III
GP90 lymphocyte homing/adhesion receptor
HUTCH-I
Hermes antigen
Hyaluronate receptor
Phagocytic glycoprotein 1
Short name:
PGP-1
Phagocytic glycoprotein I
Short name:
PGP-I
CD_antigen: CD44
Gene namesi
Name:Cd44
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2307. Cd44.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 410ExtracellularSequence analysisAdd BLAST389
Transmembranei411 – 431HelicalSequence analysisAdd BLAST21
Topological domaini432 – 503CytoplasmicSequence analysisAdd BLAST72

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000002669122 – 503CD44 antigenAdd BLAST482

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi28N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi31 ↔ 133PROSITE-ProRule annotation
Disulfide bondi56 ↔ 122PROSITE-ProRule annotation
Glycosylationi60N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi80 ↔ 100PROSITE-ProRule annotation
Glycosylationi103N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi114N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi124N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi266N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi274N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei288Sulfotyrosine1 Publication1
Glycosylationi306N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei433Phosphoserine; by PKCBy similarity1
Modified residuei447PhosphoserineCombined sources1
Modified residuei451PhosphothreonineCombined sources1
Modified residuei458PhosphoserineBy similarity1
Modified residuei467PhosphoserineCombined sources1

Post-translational modificationi

N-glycosylated.By similarity
O-glycosylated; contains chondroitin sulfate glycans which can be more or less sulfated.By similarity
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-467 (constitutive phosphorylation site), and the phosphorylation of Ser-433.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Proteoglycan, Sulfation

Proteomic databases

PRIDEiP26051.

PTM databases

iPTMnetiP26051.
PhosphoSitePlusiP26051.

Interactioni

Subunit structurei

Interacts with HA, as well as other glycosaminoglycans, collagen, laminin, and fibronectin via its N-terminal segment. Interacts with ANK, the ERM proteins (VIL2, RDX and MSN), and NF2 via its C-terminal segment. Interacts with PKN2. Interacts with TIAM1 and TIAM2. Interacts with UNC119. Interacts with PDPN (via extracellular domain); this interaction is required for PDPN-mediated directional migration and regulation of lamellipodia extension/stabilization during cell spreading and migration (By similarity).By similarity

GO - Molecular functioni

  • epidermal growth factor receptor binding Source: RGD
  • phosphoprotein binding Source: RGD
  • protein kinase binding Source: RGD

Protein-protein interaction databases

CORUMiP26051.
IntActiP26051. 1 interactor.

Structurei

3D structure databases

ProteinModelPortaliP26051.
SMRiP26051.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 124LinkPROSITE-ProRule annotationAdd BLAST90

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni228 – 410StemAdd BLAST183

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi154 – 162Arg/Lys-rich (basic)9

Domaini

The lectin-like LINK domain is responsible for hyaluronan binding.By similarity

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG003850.
InParanoidiP26051.
PhylomeDBiP26051.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiView protein in InterPro
IPR016186. C-type_lectin-like/link.
IPR001231. CD44_antigen.
IPR016187. CTDL_fold.
IPR000538. Link_dom.
PANTHERiPTHR10225:SF6. PTHR10225:SF6. 1 hit.
PfamiView protein in Pfam
PF00193. Xlink. 1 hit.
PRINTSiPR00658. CD44.
PR01265. LINKMODULE.
SMARTiView protein in SMART
SM00445. LINK. 1 hit.
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiView protein in PROSITE
PS01241. LINK_1. 1 hit.
PS50963. LINK_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 2 (identifier: P26051-1) [UniParc]FASTAAdd to basket
Also known as: Long, Meta-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDKVWWHTAW GLLCLLQLSL AQQQIDLNIT CRYAGVFHVE KNGRYSISRT
60 70 80 90 100
EAADLCEAFN TTLPTMAQME LALRKGFETC RYGFIEGHVV IPRIHPNAIC
110 120 130 140 150
AANNTGVYIL LASNTSHYDT YCFNASAPLE EDCTSVTDLP NSFDGPVTIT
160 170 180 190 200
IVNRDGTRYS KKGEYRTHQE DIDASNIIDE DVSSGSTIEK STPEGYILHT
210 220 230 240 250
DLPTSQPTGD RDDAFFIGST LATIATTPWV SAHTKQNQER TQWNPIHSNP
260 270 280 290 300
EVLLQTTTRM TDIDRNSTSA HGENWTQEPQ PPFNNHEYQD EEETPHATST
310 320 330 340 350
TWADPNSTTE EAATQKEKWF ENEWQGKNPP TPSEDSHVTE GTTASAHNNH
360 370 380 390 400
PSQRMTTQSQ EDVSWTDFFD PISHPMGQGH QTESKGHSSG NQDSGVTTTS
410 420 430 440 450
GPARRPQIPE WLIILASLLA LALILAVCIA VNSRRRCGQK KKLVINSGNG
460 470 480 490 500
TVEDRKPSEL NGEASKSQEM VHLVNKEPTE TPDQFMTADE TRNLQSVDMK

IGV
Length:503
Mass (Da):55,946
Last modified:November 1, 1997 - v2
Checksum:iFB489D009BD4EE22
GO
Isoform 1 (identifier: P26051-2) [UniParc]FASTAAdd to basket
Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     224-385: IATTPWVSAH...MGQGHQTESK → SDGDSSMDPRGGFDTVTHGSELA

Show »
Length:364
Mass (Da):39,794
Checksum:iC45DE71B059DEA5D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti74R → S in AAA97915 (Ref. 2) Curated1
Sequence conflicti74R → S in AAA92920 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005330224 – 385IATTP…QTESK → SDGDSSMDPRGGFDTVTHGS ELA in isoform 1. 2 PublicationsAdd BLAST162

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61875 mRNA. Translation: AAA53532.1.
M61874 mRNA. Translation: AAA53534.1.
U52179 mRNA. Translation: AAA97915.1.
U46957 mRNA. Translation: AAA92920.1.
PIRiB38745.
RefSeqiXP_006234693.1. XM_006234631.3.
UniGeneiRn.1120.

Genome annotation databases

GeneIDi25406.
UCSCiRGD:2307. rat. [P26051-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCD44_RAT
AccessioniPrimary (citable) accession number: P26051
Secondary accession number(s): Q99021
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: November 1, 1997
Last modified: October 25, 2017
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome