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Protein

Gamma-aminobutyric acid receptor subunit alpha-3

Gene

Gabra3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chloride channel, Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

ReactomeiREACT_293019. Ligand-gated ion channel transport.
REACT_343799. GABA A receptor activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-aminobutyric acid receptor subunit alpha-3
Alternative name(s):
GABA(A) receptor subunit alpha-3
Gene namesi
Name:Gabra3
Synonyms:Gabra-3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:95615. Gabra3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini29 – 276248ExtracellularCuratedAdd
BLAST
Transmembranei277 – 29822HelicalCuratedAdd
BLAST
Transmembranei304 – 32522HelicalCuratedAdd
BLAST
Transmembranei338 – 35922HelicalCuratedAdd
BLAST
Topological domaini360 – 45798CytoplasmicCuratedAdd
BLAST
Transmembranei458 – 47922HelicalCuratedAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence AnalysisAdd
BLAST
Chaini29 – 492464Gamma-aminobutyric acid receptor subunit alpha-3PRO_0000000438Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi63 – 631N-linked (GlcNAc...)Sequence Analysis
Glycosylationi163 – 1631N-linked (GlcNAc...)Sequence Analysis
Glycosylationi176 – 1761N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi191 ↔ 205By similarity
Glycosylationi228 – 2281N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP26049.
PaxDbiP26049.
PRIDEiP26049.

PTM databases

PhosphoSiteiP26049.

Expressioni

Gene expression databases

BgeeiP26049.
ExpressionAtlasiP26049. baseline and differential.
GenevisibleiP26049. MM.

Interactioni

Subunit structurei

Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho. Binds UBQLN1.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000062638.

Structurei

3D structure databases

ProteinModelPortaliP26049.
SMRiP26049. Positions 71-365.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG238757.
GeneTreeiENSGT00760000119010.
HOGENOMiHOG000231337.
HOVERGENiHBG051707.
InParanoidiP26049.
KOiK05175.
OrthoDBiEOG7JX342.
PhylomeDBiP26049.
TreeFamiTF315453.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR001390. GABAAa_rcpt.
IPR005433. GABBAa3_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 2 hits.
[Graphical view]
PRINTSiPR01079. GABAARALPHA.
PR01616. GABAARALPHA3.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P26049-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIITQMWHFY VTRVVLLLLI SILPGTTSQG ESRRQEPGDF VKQDIGGLSP
60 70 80 90 100
KHAPDIPDDS TDNITIFTRI LDRLLDGYDN RLRPGLGDAV TEVKTDIYVT
110 120 130 140 150
SFGPVSDTDM EYTIDVFFRQ TWHDERLKFD GPMKILPLNN LLASKIWTPD
160 170 180 190 200
TFFHNGKKSV AHNMTTPNKL LRLVDNGTLL YTMRLTIHAE CPMHLEDFPM
210 220 230 240 250
DVHACPLKFG SYAYTKAEVI YSWTLGKNKS VEVAQDGSRL NQYDLLGHVV
260 270 280 290 300
GTEIIRSSTG EYVVMTTHFH LKRKIGYFVI QTYLPCIMTV ILSQVSFWLN
310 320 330 340 350
RESVPARTVF GVTTVLTMTT LSISARNSLP KVAYATAMDW FIAVCYAFVF
360 370 380 390 400
SALIEFATVN YFTKRSWAWE GKKVPEALEM KKKTPAAPTK KNTTFNIVGT
410 420 430 440 450
TYPINLAKDT EFSTISKSAA APSASSTPTA IASPKATYVQ DSPAETKTYN
460 470 480 490
SVSKVDKISR IIFPVLFAIF NLVYWATYVN RESAIKGMIR KQ
Length:492
Mass (Da):55,398
Last modified:May 1, 1992 - v1
Checksum:i3CA193B97833CAE2
GO

RNA editingi

Edited at position 342.1 Publication
The extent of editing is low at birth but increases with age, reaching close to 100% in the adult brain.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti342 – 3421I → M in RNA edited version.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86568 mRNA. Translation: AAA37651.1.
CCDSiCCDS30187.1.
RefSeqiNP_032093.3. NM_008067.4.
XP_006527886.1. XM_006527823.2.
UniGeneiMm.102286.
Mm.447666.

Genome annotation databases

EnsembliENSMUST00000055966; ENSMUSP00000062638; ENSMUSG00000031343.
GeneIDi14396.
KEGGimmu:14396.
UCSCiuc009tkp.2. mouse.

Keywords - Coding sequence diversityi

RNA editing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86568 mRNA. Translation: AAA37651.1.
CCDSiCCDS30187.1.
RefSeqiNP_032093.3. NM_008067.4.
XP_006527886.1. XM_006527823.2.
UniGeneiMm.102286.
Mm.447666.

3D structure databases

ProteinModelPortaliP26049.
SMRiP26049. Positions 71-365.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000062638.

Chemistry

ChEMBLiCHEMBL2094133.
GuidetoPHARMACOLOGYi406.

PTM databases

PhosphoSiteiP26049.

Proteomic databases

MaxQBiP26049.
PaxDbiP26049.
PRIDEiP26049.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000055966; ENSMUSP00000062638; ENSMUSG00000031343.
GeneIDi14396.
KEGGimmu:14396.
UCSCiuc009tkp.2. mouse.

Organism-specific databases

CTDi2556.
MGIiMGI:95615. Gabra3.

Phylogenomic databases

eggNOGiNOG238757.
GeneTreeiENSGT00760000119010.
HOGENOMiHOG000231337.
HOVERGENiHBG051707.
InParanoidiP26049.
KOiK05175.
OrthoDBiEOG7JX342.
PhylomeDBiP26049.
TreeFamiTF315453.

Enzyme and pathway databases

ReactomeiREACT_293019. Ligand-gated ion channel transport.
REACT_343799. GABA A receptor activation.

Miscellaneous databases

ChiTaRSiGabra3. mouse.
NextBioi285931.
PROiP26049.
SOURCEiSearch...

Gene expression databases

BgeeiP26049.
ExpressionAtlasiP26049. baseline and differential.
GenevisibleiP26049. MM.

Family and domain databases

Gene3Di2.70.170.10. 1 hit.
InterProiIPR006028. GABAA/Glycine_rcpt.
IPR001390. GABAAa_rcpt.
IPR005433. GABBAa3_rcpt.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 2 hits.
[Graphical view]
PRINTSiPR01079. GABAARALPHA.
PR01616. GABAARALPHA3.
PR00253. GABAARECEPTR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The alpha 1, alpha 2, and alpha 3 subunits of GABAA receptors: comparison in seizure-prone and -resistant mice and during development."
    Wang J.B., Kofuji P., Fernando J.C., Moss S.J., Huganir R.L., Burt D.R.
    J. Mol. Neurosci. 3:177-184(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J and DBA/2J.
    Tissue: Brain.
  2. "GABA(A) receptor cell surface number and subunit stability are regulated by the ubiquitin-like protein Plic-1."
    Bedford F.K., Kittler J.T., Muller E., Thomas P., Uren J.M., Merlo D., Wisden W., Triller A., Smart T.G., Moss S.J.
    Nat. Neurosci. 4:908-916(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UBQLN1.
  3. "Editing modifies the GABA(A) receptor subunit alpha3."
    Ohlson J., Pedersen J.S., Haussler D., Ohman M.
    RNA 13:698-703(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: RNA EDITING OF POSITION 342.

Entry informationi

Entry nameiGBRA3_MOUSE
AccessioniPrimary (citable) accession number: P26049
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 24, 2015
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.