Gamma-aminobutyric acid receptor subunit alpha-3 precursor

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P26049 (GBRA3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 115. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Gamma-aminobutyric acid receptor subunit alpha-3

Alternative name(s):
GABA(A) receptor subunit alpha-3

Gene names

Name:	Gabra3
Synonyms:	Gabra-3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
Subunit structure	Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho. Binds UBQLN1.
Subcellular location	Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA3 sub-subfamily. [View classification]
RNA editing	Edited at position 342. The extent of editing is low at birth but increases with age, reaching close to 100% in the adult brain. Ref.4

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	RNA editing
Domain	Signal Transmembrane Transmembrane helix
Ligand	Chloride
Molecular function	Chloride channel Ion channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	gamma-aminobutyric acid signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW chloride channel complex Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GABA-A receptor activity Inferred from electronic annotation. Source: InterPro chloride channel activity Inferred from electronic annotation. Source: UniProtKB-KW extracellular ligand-gated ion channel activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 28

Potential

Chain

29 – 492

464

Gamma-aminobutyric acid receptor subunit alpha-3

PRO_0000000438

Regions

Topological domain

29 – 276

248

Extracellular Probable

Transmembrane

277 – 298

Helical; Probable

Transmembrane

304 – 325

Helical; Probable

Transmembrane

338 – 359

Helical; Probable

Topological domain

360 – 457

Cytoplasmic Probable

Transmembrane

458 – 479

Helical; Probable

Amino acid modifications

Modified residue

433

Phosphoserine Ref.3

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

163

N-linked (GlcNAc...) Potential

Glycosylation

176

N-linked (GlcNAc...) Potential

Glycosylation

228

N-linked (GlcNAc...) Potential

Disulfide bond

191 ↔ 205

By similarity

Natural variations

Natural variant

342

I → M in RNA edited version.

Sequences

Sequence

Length

Mass (Da)

Tools

P26049 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 3CA193B97833CAE2
Show »

FASTA

492

55,398

        10         20         30         40         50         60 
MIITQMWHFY VTRVVLLLLI SILPGTTSQG ESRRQEPGDF VKQDIGGLSP KHAPDIPDDS 

        70         80         90        100        110        120 
TDNITIFTRI LDRLLDGYDN RLRPGLGDAV TEVKTDIYVT SFGPVSDTDM EYTIDVFFRQ 

       130        140        150        160        170        180 
TWHDERLKFD GPMKILPLNN LLASKIWTPD TFFHNGKKSV AHNMTTPNKL LRLVDNGTLL 

       190        200        210        220        230        240 
YTMRLTIHAE CPMHLEDFPM DVHACPLKFG SYAYTKAEVI YSWTLGKNKS VEVAQDGSRL 

       250        260        270        280        290        300 
NQYDLLGHVV GTEIIRSSTG EYVVMTTHFH LKRKIGYFVI QTYLPCIMTV ILSQVSFWLN 

       310        320        330        340        350        360 
RESVPARTVF GVTTVLTMTT LSISARNSLP KVAYATAMDW FIAVCYAFVF SALIEFATVN 

       370        380        390        400        410        420 
YFTKRSWAWE GKKVPEALEM KKKTPAAPTK KNTTFNIVGT TYPINLAKDT EFSTISKSAA 

       430        440        450        460        470        480 
APSASSTPTA IASPKATYVQ DSPAETKTYN SVSKVDKISR IIFPVLFAIF NLVYWATYVN 

       490 
RESAIKGMIR KQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The alpha 1, alpha 2, and alpha 3 subunits of GABAA receptors: comparison in seizure-prone and -resistant mice and during development." Wang J.B., Kofuji P., Fernando J.C., Moss S.J., Huganir R.L., Burt D.R. J. Mol. Neurosci. 3:177-184(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J and DBA/2J. Tissue: Brain.
[2]	"GABA(A) receptor cell surface number and subunit stability are regulated by the ubiquitin-like protein Plic-1." Bedford F.K., Kittler J.T., Muller E., Thomas P., Uren J.M., Merlo D., Wisden W., Triller A., Smart T.G., Moss S.J. Nat. Neurosci. 4:908-916(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH UBQLN1.
[3]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-433, MASS SPECTROMETRY. Tissue: Brain cortex.
[4]	"Editing modifies the GABA(A) receptor subunit alpha3." Ohlson J., Pedersen J.S., Haussler D., Ohman M. RNA 13:698-703(2007) [PubMed] [Europe PMC] [Abstract] Cited for: RNA EDITING OF POSITION 342.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M86568 mRNA. Translation: AAA37651.1.
IPI	IPI00110601.
RefSeq	NP_032093.3. NM_008067.4.
UniGene	Mm.102286. Mm.447666.
3D structure databases
ProteinModelPortal	P26049.
SMR	P26049. Positions 68-364.
ModBase	Search...
PTM databases
PhosphoSite	P26049.
Proteomic databases
PaxDb	P26049.
PRIDE	P26049.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000055966; ENSMUSP00000062638; ENSMUSG00000031343.
GeneID	14396.
KEGG	mmu:14396.
UCSC	uc009tkp.2. mouse.
Organism-specific databases
CTD	2556.
MGI	MGI:95615. Gabra3.
Phylogenomic databases
eggNOG	NOG238757.
GeneTree	ENSGT00550000074441.
HOGENOM	HOG000231337.
HOVERGEN	HBG051707.
InParanoid	P26049.
KO	K05175.
OrthoDB	EOG4X0MSC.
Gene expression databases
ArrayExpress	P26049.
Bgee	P26049.
Genevestigator	P26049.
GermOnline	ENSMUSG00000031343. Mus musculus.
Family and domain databases
Gene3D	2.70.170.10. 1 hit.
InterPro	IPR006028. GABAA_rcpt. IPR001390. GABAAa_rcpt. IPR005433. GABBAa3_rcpt. IPR006202. Neur_chan_lig-bd. IPR006201. Neur_channel. IPR006029. Neurotrans-gated_channel_TM. IPR018000. Neurotransmitter_ion_chnl_CS. [Graphical view]
PANTHER	PTHR18945. PTHR18945. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. PF02932. Neur_chan_memb. 2 hits. [Graphical view]
PRINTS	PR01079. GABAARALPHA. PR01616. GABAARALPHA3. PR00253. GABAARECEPTR. PR00252. NRIONCHANNEL.
SUPFAM	SSF90112. Neu_channel_TM. 1 hit. SSF63712. Neur_chan_LBD. 1 hit.
TIGRFAMs	TIGR00860. LIC. 1 hit.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P26049.
ChEMBL	CHEMBL3387.
NextBio	285931.
SOURCE	Search...

Entry information

Entry name

GBRA3_MOUSE

Accession

Primary (citable) accession number: P26049

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	May 29, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents