P26048 (GBRA2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-aminobutyric acid receptor subunit alpha-2

Alternative name(s):
GABA(A) receptor subunit alpha-2

Gene names

Name:	Gabra2
Synonyms:	Gabra-2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
Subunit structure	Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho. Binds UBQLN1.
Subcellular location	Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA2 sub-subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Domain	Signal Transmembrane Transmembrane helix
Ligand	Chloride
Molecular function	Chloride channel Ion channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	gamma-aminobutyric acid signaling pathway Inferred from electronic annotation. Source: Compara neurotransmitter transport Inferred from electronic annotation. Source: Compara regulation of neurotransmitter levels Inferred from electronic annotation. Source: Compara
Cellular_component	axon Inferred from direct assay PubMed 9682826. Source: MGI cell junction Inferred from electronic annotation. Source: UniProtKB-KW chloride channel complex Inferred from electronic annotation. Source: UniProtKB-KW integral to synaptic vesicle membrane Inferred from electronic annotation. Source: Compara plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GABA-A receptor activity Inferred from electronic annotation. Source: InterPro benzodiazepine receptor activity Inferred from electronic annotation. Source: Compara chloride channel activity Inferred from electronic annotation. Source: UniProtKB-KW extracellular ligand-gated ion channel activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 451	423	Gamma-aminobutyric acid receptor subunit alpha-2		PRO_0000000434
Regions
	Topological domain	29 – 251	223	Extracellular Probable
	Transmembrane	252 – 273	22	Helical; Probable
	Transmembrane	279 – 300	22	Helical; Probable
	Transmembrane	313 – 334	22	Helical; Probable
	Topological domain	335 – 419	85	Cytoplasmic Probable
	Transmembrane	420 – 441	22	Helical; Probable
Amino acid modifications
	Glycosylation	38	1	N-linked (GlcNAc...) Potential
	Glycosylation	138	1	N-linked (GlcNAc...) Potential
	Disulfide bond	166 ↔ 180		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P26048 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: C5E47898963A36CF	FASTA	451	51,139
	10 20 30 40 50 60 MKTKLSTCNV WSLLLVLLVW DPVRLVLANI QEDEAKNNIT IFTRILDRLL DGYDNRLRPG 70 80 90 100 110 120 LGDSITEVFT NIYVTSFGPV SDTDMEYTID VFFRQKWKDE RLKFKGPMNI LRLNNLMASK 130 140 150 160 170 180 IWTPDTFFHN GKKSVAHNMT MPNKLLRIQD DGTLLYTMRL TVQAECPMHL EDFPMDAHSC 190 200 210 220 230 240 PLKFGSYAYT TSEVTYIWTY NASDSVQVAP DGSRLNQYDL LGQSIGKETI KSSTGEYTVM 250 260 270 280 290 300 TAHFHLKRKI GYFVIQTYLP CIMTVILSQV SFWLNRESVP ARTVFGVTTV LTMTTLSISA 310 320 330 340 350 360 RNSLPKVAYA TAMDWFIAVC YAFVFSALIE FATVNYFTKR GWAWDGKSVV NDKKKEKGSV 370 380 390 400 410 420 MIQNNAYAVA VANYAPNLSK DPVLSTISKS ATTPEPNKKP ENKPAEAKKT FNSVSKIDRM 430 440 450 SRIVFPVLFG TFNLVYWATY LNREPVLGVS P

References

[1]	"The alpha 1, alpha 2, and alpha 3 subunits of GABAA receptors: comparison in seizure-prone and -resistant mice and during development." Wang J.B., Kofuji P., Fernando J.C., Moss S.J., Huganir R.L., Burt D.R. J. Mol. Neurosci. 3:177-184(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J and DBA/2J. Tissue: Brain.
[2]	"GABA(A) receptor cell surface number and subunit stability are regulated by the ubiquitin-like protein Plic-1." Bedford F.K., Kittler J.T., Muller E., Thomas P., Uren J.M., Merlo D., Wisden W., Triller A., Smart T.G., Moss S.J. Nat. Neurosci. 4:908-916(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH UBQLN1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M86567 mRNA. Translation: AAA37650.1.
IPI	IPI00110598.
RefSeq	NP_032092.1. NM_008066.3.
UniGene	Mm.5304.
3D structure databases
ProteinModelPortal	P26048.
SMR	P26048. Positions 43-341.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000000572.
PTM databases
PhosphoSite	P26048.
Proteomic databases
PaxDb	P26048.
PRIDE	P26048.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000000572; ENSMUSP00000000572; ENSMUSG00000000560.
GeneID	14395.
KEGG	mmu:14395.
Organism-specific databases
CTD	2555.
MGI	MGI:95614. Gabra2.
Phylogenomic databases
eggNOG	NOG238757.
HOGENOM	HOG000231337.
HOVERGEN	HBG051707.
InParanoid	P26048.
KO	K05175.
OMA	TTAEPNK.
OrthoDB	EOG4ZS935.
Gene expression databases
ArrayExpress	P26048.
Bgee	P26048.
Genevestigator	P26048.
GermOnline	ENSMUSG00000000560. Mus musculus.
Family and domain databases
Gene3D	2.70.170.10. 1 hit.
InterPro	IPR006028. GABAA_rcpt. IPR001390. GABAAa_rcpt. IPR005432. GABBAa2_rcpt. IPR006202. Neur_chan_lig-bd. IPR006201. Neur_channel. IPR006029. Neurotrans-gated_channel_TM. IPR018000. Neurotransmitter_ion_chnl_CS. [Graphical view]
PANTHER	PTHR18945. PTHR18945. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. PF02932. Neur_chan_memb. 2 hits. [Graphical view]
PRINTS	PR01079. GABAARALPHA. PR01615. GABAARALPHA2. PR00253. GABAARECEPTR. PR00252. NRIONCHANNEL.
SUPFAM	SSF90112. Neu_channel_TM. 1 hit. SSF63712. Neur_chan_LBD. 1 hit.
TIGRFAMs	TIGR00860. LIC. 1 hit.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P26048.
ChEMBL	CHEMBL2705.
NextBio	285927.
SOURCE	Search...

Entry information

Entry name

GBRA2_MOUSE

Accession

Primary (citable) accession number: P26048

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	May 1, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program