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P26045 (PTN3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 145. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tyrosine-protein phosphatase non-receptor type 3

EC=3.1.3.48
Alternative name(s):
Protein-tyrosine phosphatase H1
Short name=PTP-H1
Gene names
Name:PTPN3
Synonyms:PTPH1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length913 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May act at junctions between the membrane and the cytoskeleton. Possesses tyrosine phosphatase activity.

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmcytoskeleton.

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.

Contains 1 FERM domain.

Contains 1 PDZ (DHR) domain.

Contains 1 tyrosine-protein phosphatase domain.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P26045-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P26045-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-131: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: P26045-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-131: Missing.
     335-379: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 913913Tyrosine-protein phosphatase non-receptor type 3
PRO_0000219433

Regions

Domain29 – 312284FERM
Domain510 – 58273PDZ
Domain646 – 901256Tyrosine-protein phosphatase
Region842 – 8487Substrate binding By similarity

Sites

Active site8421Phosphocysteine intermediate By similarity
Binding site8111Substrate By similarity
Binding site8861Substrate By similarity

Amino acid modifications

Modified residue3591Phosphoserine Ref.8
Modified residue3671Phosphoserine Ref.8
Modified residue3761Phosphothreonine Ref.8
Modified residue3811Phosphoserine Ref.8

Natural variations

Alternative sequence1 – 131131Missing in isoform 2 and isoform 3.
VSP_046309
Alternative sequence335 – 37945Missing in isoform 3.
VSP_046310
Natural variant771D → N.
Corresponds to variant rs35285139 [ dbSNP | Ensembl ].
VAR_055252
Natural variant901A → P. Ref.1
Corresponds to variant rs3793524 [ dbSNP | Ensembl ].
VAR_055253
Natural variant4091T → A.
Corresponds to variant rs10979858 [ dbSNP | Ensembl ].
VAR_055254
Natural variant6051F → L.
Corresponds to variant rs7859962 [ dbSNP | Ensembl ].
VAR_055255
Natural variant7631D → N.
Corresponds to variant rs10116806 [ dbSNP | Ensembl ].
VAR_055256

Experimental info

Sequence conflict8141V → I in AAA35647. Ref.1
Sequence conflict8141V → I in AAB22439. Ref.6

Secondary structure

.............................................. 913
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 5, 2009. Version 2.
Checksum: 44FBBFA35A5F2AFF

FASTA913103,990
        10         20         30         40         50         60 
MTSRLRALGG RINNIRTSEL PKEKTRSEVI CSIHFLDGVV QTFKVTKQDT GQVLLDMVHN 

        70         80         90        100        110        120 
HLGVTEKEYF GLQHDDDSVD SPRWLEASKA IRKQLKGGFP CTLHFRVRFF IPDPNTLQQE 

       130        140        150        160        170        180 
QTRHLYFLQL KMDICEGRLT CPLNSAVVLA SYAVQSHFGD YNSSIHHPGY LSDSHFIPDQ 

       190        200        210        220        230        240 
NEDFLTKVES LHEQHSGLKQ SEAESCYINI ARTLDFYGVE LHSGRDLHNL DLMIGIASAG 

       250        260        270        280        290        300 
VAVYRKYICT SFYPWVNILK ISFKRKKFFI HQRQKQAESR EHIVAFNMLN YRSCKNLWKS 

       310        320        330        340        350        360 
CVEHHTFFQA KKLLPQEKNV LSQYWTMGSR NTKKSVNNQY CKKVIGGMVW NPAMRRSLSV 

       370        380        390        400        410        420 
EHLETKSLPS RSPPITPNWR SPRLRHEIRK PRHSSADNLA NEMTYITETE DVFYTYKGSL 

       430        440        450        460        470        480 
APQDSDSEVS QNRSPHQESL SENNPAQSYL TQKSSSSVSP SSNAPGSCSP DGVDQQLLDD 

       490        500        510        520        530        540 
FHRVTKGGST EDASQYYCDK NDNGDSYLVL IRITPDEDGK FGFNLKGGVD QKMPLVVSRI 

       550        560        570        580        590        600 
NPESPADTCI PKLNEGDQIV LINGRDISEH THDQVVMFIK ASRESHSREL ALVIRRRAVR 

       610        620        630        640        650        660 
SFADFKSEDE LNQLFPEAIF PMCPEGGDTL EGSMAQLKKG LESGTVLIQF EQLYRKKPGL 

       670        680        690        700        710        720 
AITFAKLPQN LDKNRYKDVL PYDTTRVLLQ GNEDYINASY VNMEIPAANL VNKYIATQGP 

       730        740        750        760        770        780 
LPHTCAQFWQ VVWDQKLSLI VMLTTLTERG RTKCHQYWPD PPDVMNHGGF HIQCQSEDCT 

       790        800        810        820        830        840 
IAYVSREMLV TNTQTGEEHT VTHLQYVAWP DHGVPDDSSD FLEFVNYVRS LRVDSEPVLV 

       850        860        870        880        890        900 
HCSAGIGRTG VLVTMETAMC LTERNLPIYP LDIVRKMRDQ RAMMVQTSSQ YKFVCEAILR 

       910 
VYEEGLVQML DPS 

« Hide

Isoform 2 [UniParc].

Checksum: 00302B9F06B3568B
Show »

FASTA78288,814
Isoform 3 [UniParc].

Checksum: 6A9CCF192920EDE7
Show »

FASTA73783,723

References

« Hide 'large scale' references
[1]"Isolation of a cDNA clone encoding a human protein-tyrosine phosphatase with homology to the cytoskeletal-associated proteins band 4.1, ezrin, and talin."
Yang Q., Tonks N.K.
Proc. Natl. Acad. Sci. U.S.A. 88:5949-5953(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT PRO-90.
[2]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Tissue: Fetal kidney and Testis.
[3]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[6]"cDNA cloning of new protein tyrosine phosphatases in the human colon."
Arimura Y., Hinoda Y., Itoh F., Takekawa M., Tsujisaki M., Adachi M., Imai K., Yachi A.
Tumor Biol. 13:180-186(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 194-896 (ISOFORM 1).
Tissue: Colon.
[7]"Expression of cytoskeletal-associated protein tyrosine phosphatase PTPH1 mRNA in human hepatocellular carcinoma."
Ikuta S., Itoh F., Hinoda Y., Toyota M., Makiguchi Y., Imai K., Yachi A.
J. Gastroenterol. 29:727-732(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 899-913 (ISOFORM 1).
[8]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-359; SER-367; THR-376 AND SER-381, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Large-scale structural analysis of the classical human protein tyrosine phosphatome."
Barr A.J., Ugochukwu E., Lee W.H., King O.N.F., Filippakopoulos P., Alfano I., Savitsky P., Burgess-Brown N.A., Mueller S., Knapp S.
Cell 136:352-363(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.54 ANGSTROMS) OF 628-913.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M64572 mRNA. Translation: AAA35647.1.
BX648253 mRNA. No translation available.
BX648735 mRNA. No translation available.
AL359963, AL162733, AL450025 Genomic DNA. Translation: CAH71094.1.
AL450025, AL162733, AL359963 Genomic DNA. Translation: CAH73252.1.
AL162733, AL359963, AL450025 Genomic DNA. Translation: CAH73386.1.
CH471105 Genomic DNA. Translation: EAW59046.1.
BC126117 mRNA. Translation: AAI26118.1.
S39392 mRNA. Translation: AAB22439.2.
S76309 mRNA. Translation: AAB33583.1.
CCDSCCDS48000.1. [P26045-3]
CCDS48001.1. [P26045-2]
CCDS6776.1. [P26045-1]
PIRA41109.
RefSeqNP_001138840.1. NM_001145368.1.
NP_001138841.1. NM_001145369.1. [P26045-2]
NP_001138842.1. NM_001145370.1. [P26045-3]
NP_001138843.1. NM_001145371.1.
NP_002820.3. NM_002829.3. [P26045-1]
XP_006717261.1. XM_006717198.1. [P26045-1]
XP_006717262.1. XM_006717199.1. [P26045-1]
XP_006717267.1. XM_006717204.1. [P26045-2]
XP_006717269.1. XM_006717206.1. [P26045-3]
UniGeneHs.436429.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2B49X-ray1.54A628-913[»]
ProteinModelPortalP26045.
SMRP26045. Positions 31-309, 508-595, 646-904.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111740. 7 interactions.
IntActP26045. 15 interactions.
MINTMINT-246110.
STRING9606.ENSP00000262539.

Chemistry

ChEMBLCHEMBL2396509.

PTM databases

PhosphoSiteP26045.

Polymorphism databases

DMDM229462761.

Proteomic databases

MaxQBP26045.
PaxDbP26045.
PRIDEP26045.

Protocols and materials databases

DNASU5774.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000374541; ENSP00000363667; ENSG00000070159. [P26045-1]
ENST00000412145; ENSP00000416654; ENSG00000070159. [P26045-2]
ENST00000446349; ENSP00000395384; ENSG00000070159. [P26045-3]
GeneID5774.
KEGGhsa:5774.
UCSCuc004beb.2. human. [P26045-1]

Organism-specific databases

CTD5774.
GeneCardsGC09M112138.
H-InvDBHIX0025742.
HGNCHGNC:9655. PTPN3.
HPAHPA038343.
MIM176877. gene.
neXtProtNX_P26045.
PharmGKBPA33999.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5599.
HOGENOMHOG000007048.
HOVERGENHBG008322.
InParanoidP26045.
KOK18027.
OMAHFGDYNS.
OrthoDBEOG76HQ1B.
PhylomeDBP26045.
TreeFamTF315900.

Gene expression databases

ArrayExpressP26045.
BgeeP26045.
CleanExHS_PTPN3.
GenevestigatorP26045.

Family and domain databases

Gene3D1.20.80.10. 1 hit.
2.30.29.30. 1 hit.
2.30.42.10. 1 hit.
3.90.190.10. 1 hit.
InterProIPR019749. Band_41_domain.
IPR019750. Band_41_fam.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR019747. FERM_CS.
IPR000299. FERM_domain.
IPR018979. FERM_N.
IPR018980. FERM_PH-like_C.
IPR001478. PDZ.
IPR011993. PH_like_dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
IPR012151. Tyr_Pase_non-rcpt_typ-3/4.
IPR000242. Tyr_Pase_rcpt/non-rcpt.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamPF09380. FERM_C. 1 hit.
PF00373. FERM_M. 1 hit.
PF09379. FERM_N. 1 hit.
PF00595. PDZ. 1 hit.
PF00102. Y_phosphatase. 1 hit.
[Graphical view]
PIRSFPIRSF000927. Tyr-Ptase_nr3. 1 hit.
PRINTSPR00935. BAND41.
PR00700. PRTYPHPHTASE.
SMARTSM00295. B41. 1 hit.
SM00228. PDZ. 1 hit.
SM00194. PTPc. 1 hit.
[Graphical view]
SUPFAMSSF47031. SSF47031. 1 hit.
SSF50156. SSF50156. 1 hit.
SSF52799. SSF52799. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEPS00660. FERM_1. 1 hit.
PS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.
PS50106. PDZ. 1 hit.
PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50055. TYR_PHOSPHATASE_PTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPTPN3. human.
EvolutionaryTraceP26045.
GeneWikiPTPN3.
GenomeRNAi5774.
NextBio22458.
PROP26045.
SOURCESearch...

Entry information

Entry namePTN3_HUMAN
AccessionPrimary (citable) accession number: P26045
Secondary accession number(s): A0AUW9, E7EN99, E9PGU7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 5, 2009
Last modified: July 9, 2014
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM