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Protein

Ezrin

Gene

Ezr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis (By similarity).By similarity

Enzyme regulationi

A head-to-tail association, of the N-terminal and C-terminal halves results in a closed conformation (inactive form) which is incapable of actin or membrane-binding.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell shape

Enzyme and pathway databases

ReactomeiR-MMU-373752. Netrin-1 signaling.
R-MMU-437239. Recycling pathway of L1.

Protein family/group databases

TCDBi8.A.25.1.1. the ezrin/radixin/moesin (ezrin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ezrin
Alternative name(s):
Cytovillin
Villin-2
p81
Gene namesi
Name:Ezr
Synonyms:Vil2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:98931. Ezr.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3102687.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002194091 – 586EzrinAdd BLAST586

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei60N6-acetyllysineBy similarity1
Modified residuei79N6-acetyllysineBy similarity1
Modified residuei83N6-succinyllysineBy similarity1
Modified residuei116PhosphotyrosineBy similarity1
Modified residuei117S-nitrosocysteineBy similarity1
Modified residuei139N6-acetyllysineBy similarity1
Modified residuei146Phosphotyrosine; by PDGFRBy similarity1
Modified residuei354Phosphotyrosine; by PDGFRBy similarity1
Modified residuei366PhosphoserineBy similarity1
Modified residuei478PhosphotyrosineBy similarity1
Modified residuei535PhosphoserineCombined sources1
Modified residuei567Phosphothreonine; by ROCK2 and PKC/PRKCI1 Publication1

Post-translational modificationi

Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding.1 Publication
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, S-nitrosylation

Proteomic databases

EPDiP26040.
PaxDbiP26040.
PeptideAtlasiP26040.
PRIDEiP26040.

2D gel databases

REPRODUCTION-2DPAGEiP26040.

PTM databases

iPTMnetiP26040.
PhosphoSitePlusiP26040.
SwissPalmiP26040.

Expressioni

Tissue specificityi

Detected in eye lens fiber cells (PubMed:21745462). Expressed in cerebrum and cerebellum (at protein level) (PubMed:15797715). Component of the microvilli of intestinal epithelial cells.2 Publications

Developmental stagei

Detected in whole embryo from E5 with highest expression at E8, E11, E12, and E18. Expressed at E18 in brain, a clear reduction occurs after birth followed by a transient increase around 2 weeks to 1 month. Hardly detected in adult brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000052397.
CleanExiMM_EZR.
ExpressionAtlasiP26040. baseline and differential.
GenevisibleiP26040. MM.

Interactioni

Subunit structurei

Interacts with MPP5 and SLC9A3R2. Found in a complex with EZR, PODXL and SLC9A3R2 (By similarity). Interacts with MCC, PLEKHG6, PODXL, SCYL3/PACE1, SLC9A3R1 and TMEM8B. Interacts (when phosphorylated) with FES/FPS. Interacts with dimeric S100P, the interaction may be activating through unmasking of F-actin binding sites (By similarity). Identified in complexes that contain VIM, EZR, AHNAK, BFSP1, BFSP2, ANK2, PLEC, PRX and spectrin (PubMed:21745462). Detected in a complex composed of at least EZR, AHNAK, PPL and PRX (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204522. 9 interactors.
CORUMiP26040.
IntActiP26040. 13 interactors.
MINTiMINT-1708640.
STRINGi10090.ENSMUSP00000063734.

Structurei

3D structure databases

ProteinModelPortaliP26040.
SMRiP26040.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 295FERMPROSITE-ProRule annotationAdd BLAST294

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni244 – 586Interaction with SCYL3By similarityAdd BLAST343

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi115 – 120[IL]-x-C-x-x-[DE] motifBy similarity6

Domaini

The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.By similarity

Phylogenomic databases

eggNOGiKOG3529. Eukaryota.
ENOG410XQFP. LUCA.
GeneTreeiENSGT00890000139341.
HOGENOMiHOG000007113.
HOVERGENiHBG002185.
InParanoidiP26040.
KOiK08007.
OMAiARDESKN.
OrthoDBiEOG091G06UO.
PhylomeDBiP26040.
TreeFamiTF313935.

Family and domain databases

CDDicd14473. FERM_B-lobe. 1 hit.
Gene3Di1.20.80.10. 2 hits.
2.30.29.30. 1 hit.
InterProiView protein in InterPro
IPR019749. Band_41_domain.
IPR011174. ERM.
IPR011259. ERM_C_dom.
IPR000798. Ez/rad/moesin-like.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR019748. FERM_central.
IPR019747. FERM_CS.
IPR000299. FERM_domain.
IPR018979. FERM_N.
IPR018980. FERM_PH-like_C.
IPR008954. Moesin_tail.
IPR011993. PH_dom-like.
IPR029071. Ubiquitin-rel_dom.
PfamiView protein in Pfam
PF00769. ERM. 1 hit.
PF09380. FERM_C. 1 hit.
PF00373. FERM_M. 1 hit.
PF09379. FERM_N. 1 hit.
PIRSFiPIRSF002305. ERM. 1 hit.
PRINTSiPR00935. BAND41.
PR00661. ERMFAMILY.
SMARTiView protein in SMART
SM00295. B41. 1 hit.
SM01196. FERM_C. 1 hit.
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF48678. SSF48678. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiView protein in PROSITE
PS00660. FERM_1. 1 hit.
PS00661. FERM_2. 1 hit.
PS50057. FERM_3. 1 hit.

Sequencei

Sequence statusi: Complete.

P26040-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKPINVRVT TMDAELEFAI QPNTTGKQLF DQVVKTIGLR EVWYFGLQYV
60 70 80 90 100
DNKGFPTWLK LDKKVSAQEV RKENPVQFKF RAKFYPEDVA EELIQDITQK
110 120 130 140 150
LFFLQVKDGI LSDEIYCPPE TAVLLGSYAV QAKFGDYNKE MHKSGYLSSE
160 170 180 190 200
RLIPQRVMDQ HKLSRDQWED RIQVWHAEHR GMLKDSAMLE YLKIAQDLEM
210 220 230 240 250
YGINYFEIKN KKGTDLWLGV DALGLNIYEK DDKLTPKIGF PWSEIRNISF
260 270 280 290 300
NDKKFVIKPI DKKAPDFVFY APRLRINKRI LQLCMGNHEL YMRRRKPDTI
310 320 330 340 350
EVQQMKAQAR EEKHQKQLER QQLETEKKRR ETVEREKEQM LREKEELMLR
360 370 380 390 400
LQDYEQKTKR AEKELSEQIE KALQLEEERR RAQEEAERLE ADRMAALRAK
410 420 430 440 450
EELERQAQDQ IKSQEQLAAE LAEYTAKIAL LEEARRRKED EVEEWQHRAK
460 470 480 490 500
EAQDDLVKTK EELHLVMTAP PPPPPPVYEP VNYHVQEGLQ DEGAEPMGYS
510 520 530 540 550
AELSSEGILD DRNEEKRITE AEKNERVQRQ LLTLSNELSQ ARDENKRTHN
560 570 580
DIIHNENMRQ GRDKYKTLRQ IRQGNTKQRI DEFEAM
Length:586
Mass (Da):69,407
Last modified:January 23, 2007 - v3
Checksum:i5B7728F575F6DE3E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48Q → P in CAA43086 (PubMed:1955455).Curated1
Sequence conflicti325T → A in CAA43086 (PubMed:1955455).Curated1
Sequence conflicti570Q → R in BAB22341 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60671 mRNA. Translation: CAA43086.1.
AK002766 mRNA. Translation: BAB22341.1.
BC048181 mRNA. Translation: AAH48181.2.
CCDSiCCDS37428.1.
PIRiB41129.
RefSeqiNP_033536.2. NM_009510.2.
UniGeneiMm.277812.

Genome annotation databases

EnsembliENSMUST00000064234; ENSMUSP00000063734; ENSMUSG00000052397.
GeneIDi22350.
KEGGimmu:22350.
UCSCiuc008ahv.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiEZRI_MOUSE
AccessioniPrimary (citable) accession number: P26040
Secondary accession number(s): Q80ZT8, Q9DCI1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2007
Last modified: September 27, 2017
This is version 167 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot