Reviewed, UniProtKB/Swiss-Prot P26016 (FUR11_DROME)

Names and origin

Protein names

Recommended name:
    Furin-like protease 1, isoforms 1/1-X/2
      Short name=Furin-1
    EC=3.4.21.75
Alternative name(s):
    Kex2-like endoprotease 1
    dKLIP-1

Gene names

Name:	Fur1
ORF Names:	CG10772

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1269 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif By similarity.
Catalytic activity	Release of mature proteins from their proproteins by cleavage of -Arg-Xaa-Yaa-Arg-|-Zaa- bonds, where Xaa can be any amino acid and Yaa is Arg or Lys. Releases albumin, complement component C3 and vWF from their respective precursors.
Subcellular location	Golgi apparatus membrane; Multi-pass membrane protein. Ref.2
Tissue specificity	In adults, isoform 1-X is expressed in CNS, fat body and female reproductive tissues, and in embryos, in CNS, tracheal pits, hindgut, posterior spiracles and anal pads. Ref.2
Developmental stage	Isoforms 1-X and 2 are expressed in embryos, larvae, pupae and adults. Highest expression is in late embryos. Ref.2
Sequence similarities	Belongs to the peptidase S8 family. Furin subfamily.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane
Molecular function	Hydrolase Protease Serine protease
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay. Source: FlyBase
Molecular function	serine-type endopeptidase activity Inferred from direct assay. Source: FlyBase
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1-X (identifier: P26016-1)

Also known as: E;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: P26016-2)

Also known as: F;

The sequence of this isoform differs from the canonical sequence as follows:
     776-1152: Missing.
     1208-1208: Q → QQYPFPFQ

Isoform 2 (identifier: P26016-3)

Also known as: C; D; F;

The sequence of this isoform differs from the canonical sequence as follows:
     776-1152: Missing.

Isoform 1-CRR (identifier: P30430-1)

Also known as: A;

The sequence of this isoform can be found in the external entry P30430-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – ?		Potential
	Propeptide	? – 309		Potential		PRO_0000027020
	Chain	310 – 1269	960	Furin-like protease 1, isoforms 1/1-X/2		PRO_0000027021
Regions
	Transmembrane	119 – 139	21	Potential
	Transmembrane	581 – 601	21	Potential
	Transmembrane	1082 – 1102	21	Potential
	Transmembrane	1233 – 1253	21	Potential
	Region	310 – 655	346	Serine protease
Sites
	Active site	372	1	Charge relay system By similarity
	Active site	413	1	Charge relay system By similarity
	Active site	587	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	15	1	N-linked (GlcNAc...) Potential
	Glycosylation	18	1	N-linked (GlcNAc...) Potential
	Glycosylation	28	1	N-linked (GlcNAc...) Potential
	Glycosylation	108	1	N-linked (GlcNAc...) Potential
	Glycosylation	333	1	N-linked (GlcNAc...) Potential
	Glycosylation	426	1	N-linked (GlcNAc...) Potential
	Glycosylation	606	1	N-linked (GlcNAc...) Potential
	Glycosylation	727	1	N-linked (GlcNAc...) Potential
	Glycosylation	814	1	N-linked (GlcNAc...) Potential
	Glycosylation	857	1	N-linked (GlcNAc...) Potential
	Glycosylation	897	1	N-linked (GlcNAc...) Potential
	Glycosylation	908	1	N-linked (GlcNAc...) Potential
	Glycosylation	994	1	N-linked (GlcNAc...) Potential
	Disulfide bond	430 ↔ 579		By similarity
	Disulfide bond	522 ↔ 552		By similarity
	Disulfide bond	669 ↔ 695		By similarity
Natural variations
	Alternative sequence	776 – 1152	377	Missing in isoform 1 and isoform 2.		VSP_005424
	Alternative sequence	1208	1	Q → QQYPFPFQ in isoform 1.		VSP_008042

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1-X (E) [UniParc]. Last modified June 1, 2001. Version 2. Checksum: 594E641CFB024C82	FASTA	1,269	138,627
	10 20 30 40 50 60 MKNDVVRWSR QPTSNTTNSS SSSRSDSNST HKHRSKSNKL NARQLGSNAA RSCQQRSSVA 70 80 90 100 110 120 TTLEDEQQTI IECDIGNFNF DCNLFKTSFL TQHKQKRSGK SSSKSKSNRS RPLAKTKAVF 130 140 150 160 170 180 LLALQFSAVV FLCNINVGFV AGSVATAASS AGGSSPAAPS SAPSSPPTVA VPPPPPPSSA 190 200 210 220 230 240 LKVDPNGQSP VLPPYVLDYE TGGKAKLTPN NGKFGQSGSS GSNNNHIVGH YTHTWAVHIP 250 260 270 280 290 300 NGDNGMADAV AKDHGFVNLG KIFDDHYHFA HHKVSKRSLS PATHHQTRLD DDDRVHWAKQ 310 320 330 340 350 360 QRAKSRSKRD FIRMRPSRTS SRAMSMVDAM SFNDSKWPQM WYLNRGGGLD MNVIPAWKMG 370 380 390 400 410 420 ITGKGVVVTI LDDGLESDHP DIQDNYDPKA SYDVNSHDDD PMPHYDMTDS NRHGTRCAGE 430 440 450 460 470 480 VAATANNSFC AVGIAYGASV GGVRMLDGDV TDAVEARSLS LNPQHIDIYS ASWGPDDDGK 490 500 510 520 530 540 TVDGPGELAS RAFIEGTTKG RGGKGSIFIW ASGNGGREQD NCNCDGYTNS IWTLSISSAT 550 560 570 580 590 600 EEGHVPWYSE KCSSTLATTY SSGGQGEKQV VTTDLHHSCT VSHTGTSASA PLAAGIAALV 610 620 630 640 650 660 LQSNQNLTWR DLQHIVVRTA KPANLKDPSW SRNGVGRRVS HSFGYGLMDA AEMVRVARNW 670 680 690 700 710 720 KAVPEQQRCE INAPHVDKVI PPRTHITLQL TVNHCRSVNY LEHVQAKITL TSQRRGDIQL 730 740 750 760 770 780 FLRSPANTSV TLLTPRIHDN SRSGFNQWPF MSVHTWGESP QGNWQLEIHN EGRYMGHALL 790 800 810 820 830 840 REWSLIFYGT TQSIGPNDPI SVPKPSGSEA TTPNSSSTTS NLHQAYSPQY PRIPPNNFGS 850 860 870 880 890 900 SPSGGSKLPL GKVPPPNKSS YVTNNPLLNS APPKQGYQQI SATYGVILGK ANGKSNNNSK 910 920 930 940 950 960 EKTNNKGNKS NNGNKGKSGG SSGNRKEQTT QSTIIQTSTS KNKYYRISQQ QQQKNNKQDR 970 980 990 1000 1010 1020 NGVQTQRPKA NSGEKSYDEK SRKVVGEITT NSGNGSIKAA KQVKESTTTS SNSRIPKLFE 1030 1040 1050 1060 1070 1080 RYEKIQAIFP ELEPYENSSP KGKPKQAKQG KQFEVDLFKP TNGGNSRQGN TKKSPSVPPP 1090 1100 1110 1120 1130 1140 SQTMATLSIL PILPAGGSSF LPDQKILKKQ QLLMAAAGVM APAQVEVEME EVHATPDYEA 1150 1160 1170 1180 1190 1200 RKDQRKEVNG PNAQITQWDM IFYGTETPAQ PDDVANPSQS NQFNLYGNDM AHNDVEYDST 1210 1220 1230 1240 1250 1260 GQWRNMQQVG EVGMTRDHSN TAACLKWSDR KCLGLSLLFF MIMQVFFLNF KHANDNNNKN KNNIIKCIR
	Isoform 1 (F). Checksum: 0D800C3495DB918C	FASTA	899	98,797
	10 20 30 40 50 60 MKNDVVRWSR QPTSNTTNSS SSSRSDSNST HKHRSKSNKL NARQLGSNAA RSCQQRSSVA 70 80 90 100 110 120 TTLEDEQQTI IECDIGNFNF DCNLFKTSFL TQHKQKRSGK SSSKSKSNRS RPLAKTKAVF 130 140 150 160 170 180 LLALQFSAVV FLCNINVGFV AGSVATAASS AGGSSPAAPS SAPSSPPTVA VPPPPPPSSA 190 200 210 220 230 240 LKVDPNGQSP VLPPYVLDYE TGGKAKLTPN NGKFGQSGSS GSNNNHIVGH YTHTWAVHIP 250 260 270 280 290 300 NGDNGMADAV AKDHGFVNLG KIFDDHYHFA HHKVSKRSLS PATHHQTRLD DDDRVHWAKQ 310 320 330 340 350 360 QRAKSRSKRD FIRMRPSRTS SRAMSMVDAM SFNDSKWPQM WYLNRGGGLD MNVIPAWKMG 370 380 390 400 410 420 ITGKGVVVTI LDDGLESDHP DIQDNYDPKA SYDVNSHDDD PMPHYDMTDS NRHGTRCAGE 430 440 450 460 470 480 VAATANNSFC AVGIAYGASV GGVRMLDGDV TDAVEARSLS LNPQHIDIYS ASWGPDDDGK 490 500 510 520 530 540 TVDGPGELAS RAFIEGTTKG RGGKGSIFIW ASGNGGREQD NCNCDGYTNS IWTLSISSAT 550 560 570 580 590 600 EEGHVPWYSE KCSSTLATTY SSGGQGEKQV VTTDLHHSCT VSHTGTSASA PLAAGIAALV 610 620 630 640 650 660 LQSNQNLTWR DLQHIVVRTA KPANLKDPSW SRNGVGRRVS HSFGYGLMDA AEMVRVARNW 670 680 690 700 710 720 KAVPEQQRCE INAPHVDKVI PPRTHITLQL TVNHCRSVNY LEHVQAKITL TSQRRGDIQL 730 740 750 760 770 780 FLRSPANTSV TLLTPRIHDN SRSGFNQWPF MSVHTWGESP QGNWQLEIHN EGRYMAQITQ 790 800 810 820 830 840 WDMIFYGTET PAQPDDVANP SQSNQFNLYG NDMAHNDVEY DSTGQWRNMQ QQYPFPFQVG 850 860 870 880 890 EVGMTRDHSN TAACLKWSDR KCLGLSLLFF MIMQVFFLNF KHANDNNNKN KNNIIKCIR
	Isoform 2 (C) (D) (F). Checksum: 66D5C5B288C47895	FASTA	892	97,889
	10 20 30 40 50 60 MKNDVVRWSR QPTSNTTNSS SSSRSDSNST HKHRSKSNKL NARQLGSNAA RSCQQRSSVA 70 80 90 100 110 120 TTLEDEQQTI IECDIGNFNF DCNLFKTSFL TQHKQKRSGK SSSKSKSNRS RPLAKTKAVF 130 140 150 160 170 180 LLALQFSAVV FLCNINVGFV AGSVATAASS AGGSSPAAPS SAPSSPPTVA VPPPPPPSSA 190 200 210 220 230 240 LKVDPNGQSP VLPPYVLDYE TGGKAKLTPN NGKFGQSGSS GSNNNHIVGH YTHTWAVHIP 250 260 270 280 290 300 NGDNGMADAV AKDHGFVNLG KIFDDHYHFA HHKVSKRSLS PATHHQTRLD DDDRVHWAKQ 310 320 330 340 350 360 QRAKSRSKRD FIRMRPSRTS SRAMSMVDAM SFNDSKWPQM WYLNRGGGLD MNVIPAWKMG 370 380 390 400 410 420 ITGKGVVVTI LDDGLESDHP DIQDNYDPKA SYDVNSHDDD PMPHYDMTDS NRHGTRCAGE 430 440 450 460 470 480 VAATANNSFC AVGIAYGASV GGVRMLDGDV TDAVEARSLS LNPQHIDIYS ASWGPDDDGK 490 500 510 520 530 540 TVDGPGELAS RAFIEGTTKG RGGKGSIFIW ASGNGGREQD NCNCDGYTNS IWTLSISSAT 550 560 570 580 590 600 EEGHVPWYSE KCSSTLATTY SSGGQGEKQV VTTDLHHSCT VSHTGTSASA PLAAGIAALV 610 620 630 640 650 660 LQSNQNLTWR DLQHIVVRTA KPANLKDPSW SRNGVGRRVS HSFGYGLMDA AEMVRVARNW 670 680 690 700 710 720 KAVPEQQRCE INAPHVDKVI PPRTHITLQL TVNHCRSVNY LEHVQAKITL TSQRRGDIQL 730 740 750 760 770 780 FLRSPANTSV TLLTPRIHDN SRSGFNQWPF MSVHTWGESP QGNWQLEIHN EGRYMAQITQ 790 800 810 820 830 840 WDMIFYGTET PAQPDDVANP SQSNQFNLYG NDMAHNDVEY DSTGQWRNMQ QVGEVGMTRD 850 860 870 880 890 HSNTAACLKW SDRKCLGLSL LFFMIMQVFF LNFKHANDNN NKNKNNIIKC IR
	Isoform 1-CRR (A). See P30430.	FASTA	–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA sequence of a Drosophila melanogaster gene, Dfur1, encoding a protein structurally related to the subtilisin-like proprotein processing enzyme furin." Roebroek A.J.M., Pauli I.G.L., Zhang Y., van de Ven W.J.M. FEBS Lett. 289:133-137(1991) [PubMed: 1915835] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: Oregon-R. Tissue: Embryo.
[2]	"Generation of structural and functional diversity in furin-like proteins in Drosophila melanogaster by alternative splicing of the DFur1 gene." Roebroek A.J.M., Creemers J.W.M., Pauli I.G.L., Bogaert T., Van de Ven W.J.M. EMBO J. 12:1853-1870(1993) [PubMed: 8491178] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING (ISOFORMS 1-X AND 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: Oregon-R and Tuebingen. Tissue: Embryo.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: Berkeley. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X59384 mRNA. Translation: CAA42027.1. L12370, L12369 Genomic DNA. Translation: AAA28546.1. L12370, L12369 Genomic DNA. Translation: AAA28547.1. L12375 mRNA. Translation: AAA28550.1. L12376 mRNA. Translation: AAA28549.1. AE014297 Genomic DNA. Translation: AAF56463.2. AE014297 Genomic DNA. Translation: AAF56464.1. AE014297 Genomic DNA. Translation: AAN14052.1. AE014297 Genomic DNA. Translation: AAS65215.1. AE014297 Genomic DNA. Translation: AAS65216.1. AY069590 mRNA. Translation: AAL39735.1.
PIR	S17546. S35366.
RefSeq	NP_524885.1. NP_733103.1. NP_733104.1. NP_996293.1. NP_996294.1.
UniGene	Dm.1740
3D structure databases
HSSP	HSSP built from PDB template 1P8J based on UniProtKB P23188.
ModBase	Search...
Genome annotation databases
Ensembl	FBgn0004509. Drosophila melanogaster. [Contig view]
GeneID	47220.
KEGG	dme:Dmel_CG10772.
NMPDR	fig|7227.3.peg.14665.
Organism-specific databases
FlyBase	FBgn0004509. Fur1.
Phylogenomic databases
HOGENOM	P26016.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-013402-MON.
BRENDA	3.4.21.75. 48.
Gene expression databases
ArrayExpress	P26016.
GermOnline	CG10772. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000209. Pept_S8_S53. IPR015500. Peptidase_S8_subtilisin-rel. IPR002884. PrprotnconvertsP. [Graphical view]
Gene3D	G3DSA:3.40.50.200. Pept_S8_S53. 1 hit.
PANTHER	PTHR10795. SubtilSerProt. 1 hit.
Pfam	PF01483. P_proprotein. 1 hit. PF00082. Peptidase_S8. 1 hit. [Graphical view]
PRINTS	PR00723. SUBTILISIN.
ProDom	PD000717. PrprotnconvertsP. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00136. SUBTILASE_ASP. 1 hit. PS00137. SUBTILASE_HIS. 1 hit. PS00138. SUBTILASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	838963.

Entry information

Entry name

FUR11_DROME

Accession

Primary (citable) accession number: P26016
Secondary accession number(s): A4V3E2, Q05817, Q27436

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project