ID   NSS_TSWV1               Reviewed;         464 AA.
AC   P26002;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   27-MAR-2024, entry version 60.
DE   RecName: Full=Non-structural protein NS-S;
GN   Name=NSS;
OS   Tomato spotted wilt virus (strain Brazilian Br-01) (TSWV).
OC   Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC   Ellioviricetes; Bunyavirales; Tospoviridae; Orthotospovirus;
OC   Tomato spotted wilt virus.
OX   NCBI_TaxID=36413;
OH   NCBI_TaxID=133901; Frankliniella occidentalis (Western flower thrips) (Euthrips occidentalis).
OH   NCBI_TaxID=163899; Scirtothrips dorsalis (Chilli thrips).
OH   NCBI_TaxID=4081; Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OH   NCBI_TaxID=161014; Thrips tabaci.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=1693160; DOI=10.1099/0022-1317-71-5-1001;
RA   de Haan P., Wagemakers L., Peters D., Goldbach R.;
RT   "The S RNA segment of tomato spotted wilt virus has an ambisense
RT   character.";
RL   J. Gen. Virol. 71:1001-1007(1990).
RN   [2]
RP   FUNCTION.
RX   PubMed=12502849; DOI=10.1128/jvi.77.2.1329-1336.2003;
RA   Bucher E., Sijen T., De Haan P., Goldbach R., Prins M.;
RT   "Negative-strand tospoviruses and tenuiviruses carry a gene for a
RT   suppressor of gene silencing at analogous genomic positions.";
RL   J. Virol. 77:1329-1336(2003).
RN   [3]
RP   FUNCTION, AND INTERACTION WITH HOST MYC2.
RC   STRAIN=isolate TSWV-YN;
RX   PubMed=31206553; DOI=10.1371/journal.ppat.1007897;
RA   Wu X., Xu S., Zhao P., Zhang X., Yao X., Sun Y., Fang R., Ye J.;
RT   "The Orthotospovirus nonstructural protein NSs suppresses plant MYC-
RT   regulated jasmonate signaling leading to enhanced vector attraction and
RT   performance.";
RL   PLoS Pathog. 15:e1007897-e1007897(2019).
CC   -!- FUNCTION: Multifunctional protein that plays two independent roles:
CC       viral suppressor of host RNAi (VSR) and viral inducer of host
CC       attractiveness to insect vectors (VIA). Acts as a suppressor of RNA-
CC       mediated gene silencing, also known as post-transcriptional gene
CC       silencing (PTGS), a mechanism of plant viral defense that limits the
CC       accumulation of viral RNAs (PubMed:12502849). Also inhibits signal
CC       transduction by the phytohormone jasmonate, making the infected plant
CC       more attractive to aphids, which are the second host to play a role as
CC       a dissemination vector (PubMed:31206553). Acts by binding to and
CC       inhibiting MYC2 transcription factor (PubMed:31206553).
CC       {ECO:0000269|PubMed:12502849, ECO:0000269|PubMed:31206553}.
CC   -!- SUBUNIT: Interacts with host MYC2. {ECO:0000269|PubMed:31206553}.
CC   -!- SIMILARITY: Belongs to the tospovirus NS-S protein family.
CC       {ECO:0000305}.
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DR   EMBL; D00645; BAA00540.1; -; Genomic_RNA.
DR   PIR; JQ0547; MNVUWC.
DR   Proteomes; UP000006674; Genome.
DR   InterPro; IPR004915; NS-S_bunyaviral.
DR   Pfam; PF03231; Bunya_NS-S_2; 1.
DR   PIRSF; PIRSF003958; NS-S_TospoV; 1.
PE   1: Evidence at protein level;
KW   Host-virus interaction; Inhibition of host innate immune response by virus;
KW   Reference proteome; Suppressor of RNA silencing; Viral immunoevasion.
FT   CHAIN           1..464
FT                   /note="Non-structural protein NS-S"
FT                   /id="PRO_0000221984"
SQ   SEQUENCE   464 AA;  52448 MW;  AE5519179F9EF377 CRC64;
     MSSSVYESII QTRASVWGST ASGKAVVDSY WIHELGTGSQ LVQTQLYSDS RSKVVLWLYC
     KVGIFPVKKK RFLSQHVYIP IFDDIDFSIN IDNSVLALSV CSNTVNANGV KHQGHLKVLS
     PAQLHSIESI MNRSDITDRF QLQEKDIIPN DKYIEAANKG SLSCVKEHTY KIEMCYNQAL
     GKVNVLSPNR NVHEWLYSFK PNFNQVESNN RTVNSLAVKS LLMSAENNIM PNSQASTDSH
     FKLSLWLRVP KVLKQVSIQK LFKVAGDETN KTFYLSIACI PNHNSVETAL NITVICKHQL
     PIRKCKAPFE LSMMFSDLKE PYNIVHDPSY PKGSVPMLWL ETHTSLHKFF ATNLQEDVII
     YTLNNLELTP GKLDLGERTL NYSEDAYKRK YFLSKTLECL PSNTQTMSYL DSIQIPSWKI
     DFARGEIKIS PQSISVAKSL LKLDLSGIKK KESKVKEAYA SGSK
//