Elongation factor 3 - Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)

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P25997 (EF3_CANAL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 98. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Elongation factor 3
Short name=EF-3

Gene names

Name:	CEF3
Synonyms:	TEF3
ORF Names:	CaO19.11629, CaO19.4152

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida ›

Protein attributes

Sequence length	1050 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The main role of EF-3 may be to transduce nucleoside triphosphate energy into mechanical energy for translocation during translation. EF-3 stimulates EF-1-alpha-dependent binding of aminoacyl-tRNA to the ribosome.
Pathway	Protein biosynthesis; polypeptide chain elongation.
Subunit structure	Monomer.
Subcellular location	Cytoplasm Ref.5.
Miscellaneous	Has antigenic properties.
Sequence similarities	Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily. Contains 2 ABC transporter domains. Contains 7 HEAT repeats.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Elongation factor
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	cell surface Inferred from direct assay PubMed 12782322. Source: CGD cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay PubMed 19824013. Source: CGD yeast-form cell wall Inferred from direct assay PubMed 12782322. Source: CGD
Molecular_function	ATP binding Inferred from sequence or structural similarity PubMed 16254441. Source: CGD ATPase activity Inferred from direct assay Ref.2. Source: CGD GTPase activity Inferred from sequence or structural similarity PubMed 1855649. Source: CGD translation elongation factor activity Inferred from sequence or structural similarity Ref.1 Ref.4 Ref.2 PubMed 1855649. Source: CGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1050

1050

Elongation factor 3

PRO_0000093454

Regions

Repeat

46 – 83

HEAT 1

Repeat

89 – 126

HEAT 2

Repeat

127 – 165

HEAT 3

Repeat

169 – 206

HEAT 4

Repeat

208 – 244

HEAT 5

Repeat

245 – 282

HEAT 6

Repeat

288 – 326

HEAT 7

Domain

429 – 646

218

ABC transporter 1

Domain

672 – 998

327

ABC transporter 2

Nucleotide binding

466 – 473

ATP 1 By similarity

Nucleotide binding

706 – 713

ATP 2 By similarity

Compositional bias

1015 – 1037

Lys-rich (basic)

Natural variations

Natural variant

980

D → G in allele CaO19.11629.

Experimental info

Sequence conflict

111

T → A no nucleotide entry Ref.4

Sequence conflict

300

Missing in CAA77567. Ref.1

Sequence conflict

331

S → T in CAA77567. Ref.1

Sequence conflict

331

S → T in CAA78282. Ref.2

Sequence conflict

730

V → F in CAA77567. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P25997 [UniParc].

Last modified October 19, 2011. Version 2.
Checksum: 1C76CB313B595954
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FASTA

1,050

116,983

        10         20         30         40         50         60 
MSAASESKYS TEVLSELLSK LQVADNKDEA ASNISTFLNS SIVEHDVPVE FFEDLKKQIQ 

        70         80         90        100        110        120 
SKDAKVSLAA LDAYKHIAST NGLSPSVEPY VVDLVSEVAV KAGDKNKDVQ TAASDALLAI 

       130        140        150        160        170        180 
ASAITPTAVK AILPKLIDNL TNTNKWTEKV AILRAVSQLV DTAKAQIALR MPELIPVLSE 

       190        200        210        220        230        240 
SMWDTKKEVK EAATATMTKS TETIDNKDIE KFIPQLISCI AKPTEVPETV HLLGATTFVS 

       250        260        270        280        290        300 
EVTMATLSIM APLLSRGLAE RDTAIKRKAA VIVDNMCKLV EDPQIVAPFM DKLLPGLKNN 

       310        320        330        340        350        360 
FANMADPEAR EVTQRALNTL RRVGAVGEND SIPEVSTAGD IDVTLNEFNK LVADKKIAKR 

       370        380        390        400        410        420 
FDVALNYIAA IAGDLVDERE IQPEAWLQNV LPFATIFLHE KEAKEIIEEF RKRAIDNIPQ 

       430        440        450        460        470        480 
PPSFEDEEDE GEDLCNCEFS LAYGAKILLN KTQFRLKRNR RYGLCGPNGA GKSTLMRAIA 

       490        500        510        520        530        540 
NGQVEGFPTQ DECKTVYVEH DIDGTHADTT VVEFVIEDGE VGLTKDVVVD KLREFNFSDE 

       550        560        570        580        590        600 
MINMPIQSLS GGWKMKLALA RAVLKNADIL LLDEPTNHLD TVNVAWLVNY LNTCGITSII 

       610        620        630        640        650        660 
VSHDSGFLDN VTQYIIHYEG FKLRKYKGNL SEFVKKCPSA QSYYELGASD LEFRFPEPGF 

       670        680        690        700        710        720 
LEGVKTKQKA IVKVSNMSFQ YPGTSKPQIQ DINFQCSLSS RIAVIGPNGA GKSTLINVLT 

       730        740        750        760        770        780 
GELLPTTGEV YVHENCRIAY IKQHAFAHID NHLDKTPSEY IQWRFQTGED RETMDRASRQ 

       790        800        810        820        830        840 
INEEDEQNMN KIFKIEGTPR RIAGIHARRK FKNSYEYEIS WMLGENIGMK NERWVPMMSV 

       850        860        870        880        890        900 
DNTWLPRGEL METHAKLVAE VDMKEALASG QFRPLTRKEI EEHCAMLGLD AELVSHSRIR 

       910        920        930        940        950        960 
GLSGGQKVKL VLAACTWQRP HLIVLDEPTN YLDRDSLGAL SKALKAFEGG IVIITHSAEF 

       970        980        990       1000       1010       1020 
TKDLTEEVWA VLDGRMTPSD HNWVQGQGSG PRIEKKDDEE EDKFDAMGNK IAAAKKKKKL 

      1030       1040       1050 
SSAELRKKKK ERMKKKKELG DAYVSSDEEF

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and sequence analysis of the gene for translation elongation factor 3 from Candida albicans." Myers K.K., Fonzi W.A., Sypherd P.S. Nucleic Acids Res. 20:1705-1710(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.
[2]	"Isolation and sequence analysis of the gene encoding translation elongation factor 3 from Candida albicans." Didomenico B., Lupisella J.A., Sandbaken M.G., Chakraburtty K. Yeast 8:337-352(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.
[4]	"Elongation factor 3 (EF-3) from Candida albicans shows both structural and functional similarity to EF-3 from Saccharomyces cerevisiae." Colhurst D.R., Schauder B.S., Hyaes M.V., Tuite M.F. Mol. Microbiol. 6:1025-1033(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 83-148.
[5]	"Proteomics-based identification of novel Candida albicans antigens for diagnosis of systemic candidiasis in patients with underlying hematological malignancies." Pitarch A., Abian J., Carrascal M., Sanchez M., Nombela C., Gil C. Proteomics 4:3084-3106(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 301-306 AND 309-310, SUBCELLULAR LOCATION, ANTIGENICITY. Strain: SC5314 / ATCC MYA-2876. Tissue: Protoplast.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z11484 Genomic DNA. Translation: CAA77567.1. Z12822 Genomic DNA. Translation: CAA78282.1. AACQ01000202 Genomic DNA. Translation: EAK92125.1. AACQ01000200 Genomic DNA. Translation: EAK92174.1.
PIR	S25363.
RefSeq	XP_711356.1. XM_706264.1. XP_711404.1. XM_706312.1.
3D structure databases
ProteinModelPortal	P25997.
SMR	P25997. Positions 5-978.
ModBase	Search...
Protein-protein interaction databases
STRING	5476.CAL0005746.
2D gel databases
COMPLUYEAST-2DPAGE	P25997.
Proteomic databases
PRIDE	P25997.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3647006. 3647040.
KEGG	cal:CaO19.11629. cal:CaO19.4152.
Organism-specific databases
CGD	CAL0005746. CEF3. CAL0071028. CEF3.
Phylogenomic databases
eggNOG	COG0488.
KO	K03235.
Enzyme and pathway databases
UniPathway	UPA00345.
Family and domain databases
Gene3D	1.25.10.10. 1 hit.
InterPro	IPR003593. AAA+_ATPase. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR015688. Elongation_fac_3. IPR021133. HEAT_type_2. [Graphical view]
PANTHER	PTHR19211:SF5. PTHR19211:SF5. 1 hit.
Pfam	PF00005. ABC_tran. 2 hits. [Graphical view]
SMART	SM00382. AAA. 2 hits. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS00211. ABC_TRANSPORTER_1. 2 hits. PS50893. ABC_TRANSPORTER_2. 2 hits. PS50077. HEAT_REPEAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EF3_CANAL

Accession

Primary (citable) accession number: P25997
Secondary accession number(s): Q59NQ0, Q59NU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	October 19, 2011
Last modified:	April 3, 2013
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Candida albicans

Candida albicans: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents