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Protein

Carbamoyl-phosphate synthase pyrimidine-specific large chain

Gene

pyrAB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 4 Mg2+ or Mn2+ ions per subunit.By similarity

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase pyrimidine-specific large chain (pyrAB), Carbamoyl-phosphate synthase pyrimidine-specific small chain (pyrAA)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi284Magnesium or manganese 1By similarity1
Metal bindingi298Magnesium or manganese 1By similarity1
Metal bindingi298Magnesium or manganese 2By similarity1
Metal bindingi300Magnesium or manganese 2By similarity1
Metal bindingi820Magnesium or manganese 3By similarity1
Metal bindingi832Magnesium or manganese 3By similarity1
Metal bindingi832Magnesium or manganese 4By similarity1
Metal bindingi834Magnesium or manganese 4By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi159 – 216ATPBy similarityAdd BLAST58
Nucleotide bindingi697 – 754ATPBy similarityAdd BLAST58

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPyrimidine biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU15520-MONOMER
MetaCyc:BSU15520-MONOMER
SABIO-RKiP25994
UniPathwayiUPA00070; UER00115

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase pyrimidine-specific large chain (EC:6.3.5.5)
Alternative name(s):
Carbamoyl-phosphate synthetase ammonia chain
Gene namesi
Name:pyrAB
Ordered Locus Names:BSU15520
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001449911 – 1071Carbamoyl-phosphate synthase pyrimidine-specific large chainAdd BLAST1071

Proteomic databases

PaxDbiP25994
PRIDEiP25994

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.

Protein-protein interaction databases

IntActiP25994, 1 interactor
STRINGi224308.Bsubs1_010100008576

Structurei

3D structure databases

ProteinModelPortaliP25994
SMRiP25994
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini133 – 327ATP-grasp 1Add BLAST195
Domaini671 – 861ATP-grasp 2Add BLAST191
Domaini930 – 1071MGS-likePROSITE-ProRule annotationAdd BLAST142

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 401Carboxyphosphate synthetic domainAdd BLAST401
Regioni402 – 546Oligomerization domainAdd BLAST145
Regioni547 – 929Carbamoyl phosphate synthetic domainAdd BLAST383
Regioni930 – 1071Allosteric domainAdd BLAST142

Sequence similaritiesi

Belongs to the CarB family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105CU6 Bacteria
COG0458 LUCA
HOGENOMiHOG000234583
InParanoidiP25994
KOiK01955
OMAiAVFPFNK
PhylomeDBiP25994

Family and domain databases

CDDicd01424 MGS_CPS_II, 1 hit
Gene3Di1.10.1030.10, 1 hit
3.40.50.1380, 1 hit
HAMAPiMF_01210_A CPSase_L_chain_A, 1 hit
MF_01210_B CPSase_L_chain_B, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR006275 CarbamoylP_synth_lsu
IPR005480 CarbamoylP_synth_lsu_oligo
IPR036897 CarbamoylP_synth_lsu_oligo_sf
IPR005479 CbamoylP_synth_lsu-like_ATP-bd
IPR005483 CbamoylP_synth_lsu_CPSase_dom
IPR011607 MGS-like_dom
IPR036914 MGS-like_dom_sf
IPR033937 MGS_CPS_CarB
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF02786 CPSase_L_D2, 2 hits
PF02787 CPSase_L_D3, 1 hit
PF02142 MGS, 1 hit
PRINTSiPR00098 CPSASE
SMARTiView protein in SMART
SM01096 CPSase_L_D3, 1 hit
SM00851 MGS, 1 hit
SUPFAMiSSF48108 SSF48108, 1 hit
SSF52335 SSF52335, 1 hit
SSF52440 SSF52440, 2 hits
TIGRFAMsiTIGR01369 CPSaseII_lrg, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 2 hits
PS00866 CPSASE_1, 2 hits
PS00867 CPSASE_2, 2 hits
PS51855 MGS, 1 hit

Sequencei

Sequence statusi: Complete.

P25994-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKRVDINKI LVIGSGPIII GQAAEFDYAG TQACLALKEE GYEVILVNSN
60 70 80 90 100
PATIMTDTEM ADRVYIEPLT PEFLTRIIRK ERPDAILPTL GGQTGLNLAV
110 120 130 140 150
ELSERGVLAE CGVEVLGTKL SAIQQAEDRD LFRTLMNELN EPVPESEIIH
160 170 180 190 200
SLEEAEKFVS QIGFPVIVRP AYTLGGTGGG ICSNETELKE IVENGLKLSP
210 220 230 240 250
VHQCLLEKSI AGYKEIEYEV MRDSQDHAIV VCNMENIDPV GIHTGDSIVV
260 270 280 290 300
APSQTLSDRE YQLLRNVSLK LIRALGIEGG CNVQLALDPD SFQYYIIEVN
310 320 330 340 350
PRVSRSSALA SKATGYPIAK LAAKIAVGLS LDEMMNPVTG KTYAAFEPAL
360 370 380 390 400
DYVVSKIPRW PFDKFESANR KLGTQMKATG EVMAIGRTLE ESLLKAVRSL
410 420 430 440 450
EADVYHLELK DAADISDELL EKRIKKAGDE RLFYLAEAYR RGYTVEDLHE
460 470 480 490 500
FSAIDVFFLH KLFGIVQFEK ELKANAGDTD VLRRAKELGF SDQYISREWK
510 520 530 540 550
MKESELYSLR KQAGIAPVFK MVDTCAAEFE SETPYFYSTY EEENESVVTD
560 570 580 590 600
KKSVMVLGSG PIRIGQGVEF DYATVHSVWA IKQAGYEAII VNNNPETVST
610 620 630 640 650
DFSISDKLYF EPLTIEDVMH IIDLEQPMGV VVQFGGQTAI NLADELSARG
660 670 680 690 700
VKILGTSLED LDRAEDRDKF EQALGELGVP QPLGKTATSV NQAVSIASDI
710 720 730 740 750
GYPVLVRPSY VLGGRAMEIV YHEEELLHYM KNAVKINPQH PVLIDRYLTG
760 770 780 790 800
KEIEVDAVSD GETVVIPGIM EHIERAGVHS GDSIAVYPPQ SLTEDIKKKI
810 820 830 840 850
EQYTIALAKG LNIVGLLNIQ FVLSQGEVYV LEVNPRSSRT VPFLSKITGI
860 870 880 890 900
PMANLATKII LGQKLAAFGY TEGLQPEQQG VFVKAPVFSF AKLRRVDITL
910 920 930 940 950
GPEMKSTGEV MGKDSTLEKA LYKALIASGI QIPNYGSVLL TVADKDKEEG
960 970 980 990 1000
LAIAKRFHAI GYNILATEGT AGYLKEASIP AKVVGKIGQD GPNLLDVIRN
1010 1020 1030 1040 1050
GEAQFVINTL TKGKQPARDG FRIRRESVEN GVACLTSLDT AEAILRVLES
1060 1070
MTFRADQMPA VNTNQEAAVT I
Length:1,071
Mass (Da):117,650
Last modified:May 1, 1992 - v1
Checksum:i195E33CFC5C6222C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59757 Genomic DNA Translation: AAA21270.1
AL009126 Genomic DNA Translation: CAB13426.1
PIRiF39845
RefSeqiNP_389435.1, NC_000964.3
WP_003232113.1, NZ_JNCM01000035.1

Genome annotation databases

EnsemblBacteriaiCAB13426; CAB13426; BSU15520
GeneIDi936608
KEGGibsu:BSU15520
PATRICifig|224308.179.peg.1691

Similar proteinsi

Entry informationi

Entry nameiCARB_BACSU
AccessioniPrimary (citable) accession number: P25994
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: March 28, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health