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Protein

Carbamoyl-phosphate synthase pyrimidine-specific small chain

Gene

pyrAA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase pyrimidine-specific large chain (pyrAB), Carbamoyl-phosphate synthase pyrimidine-specific small chain (pyrAA)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei246NucleophileBy similarity1
Active sitei329By similarity1
Active sitei331By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPyrimidine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU15510-MONOMER.
SABIO-RKiP25993.
UniPathwayiUPA00070; UER00115.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase pyrimidine-specific small chain (EC:6.3.5.5)
Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain
Gene namesi
Name:pyrAA
Ordered Locus Names:BSU15510
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001122551 – 364Carbamoyl-phosphate synthase pyrimidine-specific small chainAdd BLAST364

Proteomic databases

PaxDbiP25993.
PRIDEiP25993.

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100008571.

Structurei

3D structure databases

ProteinModelPortaliP25993.
SMRiP25993.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini171 – 356Glutamine amidotransferase type-1Add BLAST186

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 167CPSaseAdd BLAST167

Sequence similaritiesi

Belongs to the CarA family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105C1M. Bacteria.
COG0505. LUCA.
HOGENOMiHOG000038087.
InParanoidiP25993.
KOiK01956.
OMAiCFNTGMT.
PhylomeDBiP25993.

Family and domain databases

CDDicd01744. GATase1_CPSase. 1 hit.
Gene3Di3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPiMF_01209. CPSase_S_chain. 1 hit.
InterProiView protein in InterPro
IPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR029062. Class_I_gatase-like.
IPR035686. CPSase_GATase1.
IPR017926. GATASE.
PfamiView protein in Pfam
PF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
SMARTiView protein in SMART
SM01097. CPSase_sm_chain. 1 hit.
SUPFAMiSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEiView protein in PROSITE
PS51273. GATASE_TYPE_1. 1 hit.

Sequencei

Sequence statusi: Complete.

P25993-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRRLVLENG AVFEGEAFGS LEHNMGEVVF NTGMTGYQEI LSDPSYCGQI
60 70 80 90 100
VTLTYPLIGN YGINRDDFES ITPFVKGLII KELCELPSNW RSAYTLDEYL
110 120 130 140 150
KMKNIPGLQG IDTRKLTRMI RTAGALKGTF ASSDEDIEAV LKRLNETELP
160 170 180 190 200
RNQVSQVSAK TAYPSPGRGK RIVLVDFGMK HGILRELNKR KCDVIVVPYN
210 220 230 240 250
ITAEEVLQLK PDGIMLSNGP GDPKDVPEAI EMIKGVLGKV PLFGICLGHQ
260 270 280 290 300
LFALACGANT EKMKFGHRGS NHPVKELATG KVALTSQNHG YTVSSISKTE
310 320 330 340 350
LEVTHIAIND DTIEGLKHKT LPAFTVQYHP EASPGPEDAN HLFDRFIEMI
360
ETTEKEGEAV CQNA
Length:364
Mass (Da):40,119
Last modified:May 1, 1992 - v1
Checksum:i676304F876709FC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59757 Genomic DNA. Translation: AAA21269.1.
AL009126 Genomic DNA. Translation: CAB13425.1.
PIRiE39845.
RefSeqiNP_389434.1. NC_000964.3.
WP_003232115.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13425; CAB13425; BSU15510.
GeneIDi937368.
KEGGibsu:BSU15510.
PATRICifig|224308.179.peg.1690.

Similar proteinsi

Entry informationi

Entry nameiCARA_BACSU
AccessioniPrimary (citable) accession number: P25993
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: August 30, 2017
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families