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Protein

Carbamoyl-phosphate synthase pyrimidine-specific small chain

Gene

pyrAA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase pyrimidine-specific large chain (pyrAB), Carbamoyl-phosphate synthase pyrimidine-specific small chain (pyrAA)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei246 – 2461NucleophileBy similarity
Active sitei329 – 3291By similarity
Active sitei331 – 3311By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU15510-MONOMER.
SABIO-RKP25993.
UniPathwayiUPA00070; UER00115.

Protein family/group databases

MEROPSiC26.A33.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase pyrimidine-specific small chain (EC:6.3.5.5)
Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain
Gene namesi
Name:pyrAA
Ordered Locus Names:BSU15510
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 364364Carbamoyl-phosphate synthase pyrimidine-specific small chainPRO_0000112255Add
BLAST

Proteomic databases

PaxDbiP25993.

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100008571.

Structurei

3D structure databases

ProteinModelPortaliP25993.
SMRiP25993. Positions 1-356.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini171 – 356186Glutamine amidotransferase type-1Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 167167CPSaseAdd
BLAST

Sequence similaritiesi

Belongs to the CarA family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105C1M. Bacteria.
COG0505. LUCA.
HOGENOMiHOG000038087.
InParanoidiP25993.
KOiK01956.
OMAiCFSVQYH.
PhylomeDBiP25993.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPiMF_01209. CPSase_S_chain. 1 hit.
InterProiIPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
[Graphical view]
PfamiPF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SMARTiSM01097. CPSase_sm_chain. 1 hit.
[Graphical view]
SUPFAMiSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25993-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRRLVLENG AVFEGEAFGS LEHNMGEVVF NTGMTGYQEI LSDPSYCGQI
60 70 80 90 100
VTLTYPLIGN YGINRDDFES ITPFVKGLII KELCELPSNW RSAYTLDEYL
110 120 130 140 150
KMKNIPGLQG IDTRKLTRMI RTAGALKGTF ASSDEDIEAV LKRLNETELP
160 170 180 190 200
RNQVSQVSAK TAYPSPGRGK RIVLVDFGMK HGILRELNKR KCDVIVVPYN
210 220 230 240 250
ITAEEVLQLK PDGIMLSNGP GDPKDVPEAI EMIKGVLGKV PLFGICLGHQ
260 270 280 290 300
LFALACGANT EKMKFGHRGS NHPVKELATG KVALTSQNHG YTVSSISKTE
310 320 330 340 350
LEVTHIAIND DTIEGLKHKT LPAFTVQYHP EASPGPEDAN HLFDRFIEMI
360
ETTEKEGEAV CQNA
Length:364
Mass (Da):40,119
Last modified:May 1, 1992 - v1
Checksum:i676304F876709FC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59757 Genomic DNA. Translation: AAA21269.1.
AL009126 Genomic DNA. Translation: CAB13425.1.
PIRiE39845.
RefSeqiNP_389434.1. NC_000964.3.
WP_003232115.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13425; CAB13425; BSU15510.
GeneIDi937368.
KEGGibsu:BSU15510.
PATRICi18974909. VBIBacSub10457_1646.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59757 Genomic DNA. Translation: AAA21269.1.
AL009126 Genomic DNA. Translation: CAB13425.1.
PIRiE39845.
RefSeqiNP_389434.1. NC_000964.3.
WP_003232115.1. NZ_JNCM01000035.1.

3D structure databases

ProteinModelPortaliP25993.
SMRiP25993. Positions 1-356.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100008571.

Protein family/group databases

MEROPSiC26.A33.

Proteomic databases

PaxDbiP25993.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13425; CAB13425; BSU15510.
GeneIDi937368.
KEGGibsu:BSU15510.
PATRICi18974909. VBIBacSub10457_1646.

Phylogenomic databases

eggNOGiENOG4105C1M. Bacteria.
COG0505. LUCA.
HOGENOMiHOG000038087.
InParanoidiP25993.
KOiK01956.
OMAiCFSVQYH.
PhylomeDBiP25993.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00115.
BioCyciBSUB:BSU15510-MONOMER.
SABIO-RKP25993.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPiMF_01209. CPSase_S_chain. 1 hit.
InterProiIPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
[Graphical view]
PfamiPF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SMARTiSM01097. CPSase_sm_chain. 1 hit.
[Graphical view]
SUPFAMiSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCARA_BACSU
AccessioniPrimary (citable) accession number: P25993
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: September 7, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.