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Protein

Thymidine kinase

Gene

TK

Organism
Meleagrid herpesvirus 1 (MeHV-1) (Turkey herpesvirus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of the gamma-phospho group of ATP to thymidine to generate dTMP in the salvage pathway of pyrimidine synthesis. The dTMP serves as a substrate for DNA polymerase during viral DNA replication. Allows the virus to be reactivated and to grow in non-proliferative cells lacking a high concentration of phosphorylated nucleic acid precursors.UniRule annotation

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei45Proton acceptorUniRule annotation1
Binding sitei86SubstrateUniRule annotation1
Binding sitei176ATPUniRule annotation1
Binding sitei182SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 24ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinaseUniRule annotation (EC:2.7.1.21UniRule annotation)
Gene namesi
Name:TKUniRule annotation
OrganismiMeleagrid herpesvirus 1 (MeHV-1) (Turkey herpesvirus)
Taxonomic identifieri37108 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeMardivirus
Virus hostiGallus gallus (Chicken) [TaxID: 9031]
Meleagris gallopavo (Common turkey) [TaxID: 9103]

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001750801 – 350Thymidine kinaseAdd BLAST350

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP25987.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the herpesviridae thymidine kinase family.UniRule annotation

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_04029. HSV_KITH. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P25987-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALPRRPPTL TRVYLDGPFG IGKTSILNAM PDHTPDGAPI LKVYEPMKYW
60 70 80 90 100
RCQSTDLVVA ANETPERRRG GALSRFQSDM IMASIQARFA DPYLLFHERL
110 120 130 140 150
SSKCRGKIEI CDTPAIILML DRHPVAAILC FPITRYLLGE YSLEMLISSI
160 170 180 190 200
IRLPLESPGC NLTVTILPDE KEHVNRICSR DRPGETADRN MLRTLNAVYA
210 220 230 240 250
SLVDTVKYAN LTCPYEKESW EMEWLGLPWF EESLLEEFIS RPRPVICSRT
260 270 280 290 300
RMPLDRTLLA IFKRKELCSE NGELLTQYSW ILWGLLTKLH TINVELFDIS
310 320 330 340 350
GMSRRECASA IMHTMPERLS TLASWNDLCE LEDDVISYNK GMCNEVGASR
Length:350
Mass (Da):39,968
Last modified:May 1, 1992 - v1
Checksum:iAD3B8F96E431EB83
GO

Sequence databases

RefSeqiNP_073316.1. NC_002641.1.

Genome annotation databases

GeneIDi918500.
KEGGivg:918500.

Cross-referencesi

Sequence databases

RefSeqiNP_073316.1. NC_002641.1.

3D structure databases

ProteinModelPortaliP25987.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi918500.
KEGGivg:918500.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_04029. HSV_KITH. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKITH_MEHV1
AccessioniPrimary (citable) accession number: P25987
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: October 5, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.