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Protein

Nucleolar transcription factor 1

Gene

Ubtf

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi112 – 180HMG box 1PROSITE-ProRule annotationAdd BLAST69
DNA bindingi196 – 264HMG box 2PROSITE-ProRule annotationAdd BLAST69
DNA bindingi298 – 362HMG box 3PROSITE-ProRule annotationAdd BLAST65
DNA bindingi407 – 475HMG box 4PROSITE-ProRule annotationAdd BLAST69
DNA bindingi482 – 549HMG box 5PROSITE-ProRule annotationAdd BLAST68
DNA bindingi568 – 634HMG box 6PROSITE-ProRule annotationAdd BLAST67

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-RNO-73728 RNA Polymerase I Promoter Opening
R-RNO-73762 RNA Polymerase I Transcription Initiation
R-RNO-73772 RNA Polymerase I Promoter Escape
R-RNO-73777 RNA Polymerase I Chain Elongation
R-RNO-73863 RNA Polymerase I Transcription Termination

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolar transcription factor 1
Alternative name(s):
Upstream-binding factor 1
Short name:
UBF-1
Gene namesi
Name:Ubtf
Synonyms:Tcfubf, Ubf-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi3927 Ubtf

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000486271 – 764Nucleolar transcription factor 1Add BLAST764

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei201PhosphothreonineBy similarity1
Modified residuei273PhosphoserineBy similarity1
Modified residuei336PhosphoserineBy similarity1
Modified residuei364PhosphoserineBy similarity1
Modified residuei389PhosphoserineBy similarity1
Modified residuei412PhosphoserineBy similarity1
Modified residuei433PhosphoserineBy similarity1
Modified residuei435PhosphoserineBy similarity1
Modified residuei484PhosphoserineBy similarity1
Modified residuei495PhosphoserineBy similarity1
Modified residuei546PhosphoserineBy similarity1
Modified residuei584PhosphoserineBy similarity1
Modified residuei638PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated and activated by PIK3CA.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP25977
PRIDEiP25977

PTM databases

iPTMnetiP25977
PhosphoSitePlusiP25977

Expressioni

Gene expression databases

BgeeiENSRNOG00000020937
GenevisibleiP25977 RN

Interactioni

Subunit structurei

Homodimer (PubMed:10099786). Interacts with TBP (By similarity). Interacts with TAF1A (By similarity). Interacts with RASL11A (By similarity). Binds to IRS1 and PIK3CA (By similarity). Interacts with DHX33 (By similarity). Interacts with PHF6 (By similarity). Interacts with CEBPA (isoform 1 and isoform 4) (PubMed:20075868). Interacts with DDX11 (By similarity). Interacts with NOP53 (By similarity).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Runx2Q9Z2J92EBI-15620127,EBI-6119952

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-60265N
IntActiP25977, 2 interactors
STRINGi10116.ENSRNOP00000063740

Structurei

3D structure databases

ProteinModelPortaliP25977
SMRiP25977
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi675 – 764Asp/Glu/Ser-rich (acidic)Add BLAST90

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0381 Eukaryota
COG5648 LUCA
GeneTreeiENSGT00530000063934
HOGENOMiHOG000232068
HOVERGENiHBG008708
InParanoidiP25977
KOiK09273
OMAiKAMEATW
OrthoDBiEOG091G0GL0
PhylomeDBiP25977
TreeFamiTF328989

Family and domain databases

Gene3Di1.10.30.10, 6 hits
InterProiView protein in InterPro
IPR029215 HMG_box_5
IPR009071 HMG_box_dom
IPR036910 HMG_box_dom_sf
PfamiView protein in Pfam
PF00505 HMG_box, 3 hits
PF09011 HMG_box_2, 1 hit
PF14887 HMG_box_5, 1 hit
SMARTiView protein in SMART
SM00398 HMG, 6 hits
SUPFAMiSSF47095 SSF47095, 6 hits
PROSITEiView protein in PROSITE
PS50118 HMG_BOX_2, 6 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform UBF1 (identifier: P25977-1) [UniParc]FASTAAdd to basket
Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNGEADCPTD LEMAAPKGQD RWSQEDMLTL LECMKNNLPS NDSSKFKTTE
60 70 80 90 100
SHMDWEKVAF KDFSGDMCKL KWVEISNEVR KFRTLTELIL DAQEHVKNPY
110 120 130 140 150
KGKKLKKHPD FPKKPLTPYF RFFMEKRAKY AKLHPEMSNL DLTKILSKKY
160 170 180 190 200
KELPEKKKMK YIQDFQREKQ EFERNLARFR EDHPDLIQNA KKSDIPEKPK
210 220 230 240 250
TPQQLWYTHE KKVYLKVRPD ATTKEVKDSL GKQWSQLSDK KRLKWIHKAL
260 270 280 290 300
EQRKEYEEIM RDYIQKHPEL NISEEGITKS TLTKAERQLK DKFDGRPTKP
310 320 330 340 350
PPNSYSLYCA ELMANMKDVP STERMVLCSQ QWKLLSQKEK DAYHKKCDQK
360 370 380 390 400
KKDYEVELLR FLESLPEEEQ QRVLGEEKML NINKKQTTSP ASKKPSQEGG
410 420 430 440 450
KGGSEKPKRP VSAMFIFSEE KRRQLQEERP ELSESELTRL LARMWNDLSE
460 470 480 490 500
KKKAKYKARE AALKAQSERK PGGEREDRGK LPESPKRAEE IWQQSVIGDY
510 520 530 540 550
LARFKNDRVK ALKAMEMTWN NMEKKEKLMW IKKAAEDQKR YERELSEMRA
560 570 580 590 600
PPAATNSSKK MKFQGEPKKP PMNGYQKFSQ ELLSNGELNH LPLKERMVEI
610 620 630 640 650
GSRWQRISQS QKEHYKKLAE EQQRQYKVHL DLWVKSLSPQ DRAAYKEYIS
660 670 680 690 700
NKRKNMTKLR GPNPKSSRTT LQSKSESEED DDEEDDDDDD EEEEEDDENG
710 720 730 740 750
DSSEDGGDSS ESSSEDESED GDENEDDDDD EDDDEDDDED EDNESEGSSS
760
SSSSSGDSSD SDSN
Length:764
Mass (Da):89,437
Last modified:May 1, 1992 - v1
Checksum:iE9D3371615DB0534
GO
Isoform UBF2 (identifier: P25977-2) [UniParc]FASTAAdd to basket
Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     221-257: Missing.

Show »
Length:727
Mass (Da):84,968
Checksum:iA37D049F18B2AB2D
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002195221 – 257Missing in isoform UBF2. CuratedAdd BLAST37

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61726 mRNA No translation available.
M61725 mRNA No translation available.
PIRiA40439
B40439
RefSeqiNP_001099193.2, NM_001105723.3 [P25977-2]
NP_001121162.1, NM_001127690.2 [P25977-1]
XP_006247324.1, XM_006247262.3 [P25977-1]
XP_006247325.1, XM_006247263.3 [P25977-1]
XP_006247326.1, XM_006247264.3 [P25977-1]
XP_008766174.1, XM_008767952.2 [P25977-2]
UniGeneiRn.22469

Genome annotation databases

EnsembliENSRNOT00000048418; ENSRNOP00000044305; ENSRNOG00000020937 [P25977-2]
ENSRNOT00000064283; ENSRNOP00000063740; ENSRNOG00000020937 [P25977-1]
GeneIDi25574
KEGGirno:25574
UCSCiRGD:3927 rat [P25977-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUBF1_RAT
AccessioniPrimary (citable) accession number: P25977
Secondary accession number(s): P25978
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: March 28, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health