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Protein

Nucleolar transcription factor 1

Gene

Ubtf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi112 – 180HMG box 1PROSITE-ProRule annotationAdd BLAST69
DNA bindingi196 – 264HMG box 2PROSITE-ProRule annotationAdd BLAST69
DNA bindingi298 – 362HMG box 3PROSITE-ProRule annotationAdd BLAST65
DNA bindingi407 – 475HMG box 4PROSITE-ProRule annotationAdd BLAST69
DNA bindingi482 – 549HMG box 5PROSITE-ProRule annotationAdd BLAST68
DNA bindingi568 – 634HMG box 6PROSITE-ProRule annotationAdd BLAST67

GO - Molecular functioni

GO - Biological processi

  • chromatin silencing at rDNA Source: MGI
  • positive regulation of transcription from RNA polymerase I promoter Source: UniProtKB
  • transcription elongation from RNA polymerase I promoter Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleolar transcription factor 1
Alternative name(s):
Upstream-binding factor 1
Short name:
UBF-1
Gene namesi
Name:Ubtf
Synonyms:Tcfubf, Ubf-1, Ubf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:98512. Ubtf.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: UniProtKB
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000486261 – 765Nucleolar transcription factor 1Add BLAST765

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei201PhosphothreonineCombined sources1
Modified residuei273Phosphoserine1 Publication1
Modified residuei336Phosphoserine1 Publication1
Modified residuei364Phosphoserine1 Publication1
Modified residuei389Phosphoserine1 Publication1
Modified residuei412Phosphoserine1 Publication1
Modified residuei433Phosphoserine1 Publication1
Modified residuei435PhosphoserineBy similarity1
Modified residuei484PhosphoserineCombined sources1 Publication1
Modified residuei495PhosphoserineBy similarity1
Modified residuei546Phosphoserine1 Publication1
Modified residuei584Phosphoserine1 Publication1
Modified residuei638Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated and activated by PIK3CA.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP25976.
MaxQBiP25976.
PaxDbiP25976.
PeptideAtlasiP25976.
PRIDEiP25976.

PTM databases

iPTMnetiP25976.
PhosphoSitePlusiP25976.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with TBP (By similarity). Interacts with TAF1A (By similarity). Interacts with RASL11A (PubMed:20168301). Binds to IRS1 and PIK3CA (PubMed:15197263). Interacts with DHX33 (PubMed:21930779). Interacts with PHF6 (By similarity). Interacts with CEBPA (isoform 1 and isoform 4) (By similarity). Interacts with DDX11 (By similarity).By similarity3 Publications

Protein-protein interaction databases

DIPiDIP-29977N.
IntActiP25976. 7 interactors.
MINTiMINT-4084181.
STRINGi10090.ENSMUSP00000078539.

Structurei

Secondary structure

1765
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni293 – 295Combined sources3
Helixi304 – 315Combined sources12
Helixi321 – 334Combined sources14
Helixi337 – 364Combined sources28
Helixi367 – 374Combined sources8
Turni375 – 379Combined sources5
Helixi413 – 420Combined sources8
Helixi422 – 428Combined sources7
Helixi434 – 447Combined sources14
Helixi450 – 463Combined sources14
Turni464 – 467Combined sources4
Beta strandi572 – 574Combined sources3
Helixi575 – 585Combined sources11
Turni587 – 590Combined sources4
Helixi593 – 604Combined sources12
Helixi609 – 636Combined sources28
Helixi641 – 651Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V63NMR-A567-654[»]
1V64NMR-A288-382[»]
1WGFNMR-A394-470[»]
ProteinModelPortaliP25976.
SMRiP25976.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25976.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi675 – 765Asp/Glu/Ser-rich (acidic)Add BLAST91

Sequence similaritiesi

Contains 6 HMG box DNA-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0381. Eukaryota.
COG5648. LUCA.
HOGENOMiHOG000232068.
HOVERGENiHBG008708.
InParanoidiP25976.
PhylomeDBiP25976.

Family and domain databases

Gene3Di1.10.30.10. 6 hits.
InterProiIPR029215. HMG_box_5.
IPR009071. HMG_box_dom.
[Graphical view]
PfamiPF00505. HMG_box. 3 hits.
PF09011. HMG_box_2. 1 hit.
PF14887. HMG_box_5. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 6 hits.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 6 hits.
PROSITEiPS50118. HMG_BOX_2. 6 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform UBF1 (identifier: P25976-1) [UniParc]FASTAAdd to basket
Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNGEADCPTD LEMAAPKGQD RWSQEDMLTL LECMKNNLPS NDSSKFKTTE
60 70 80 90 100
SHMDWEKVAF KDFSGDMCKL KWVEISNEVR KFRTLTELIL DAQEHVKNPY
110 120 130 140 150
KGKKLKKHPD FPKKPLTPYF RFFMEKRAKY AKLHPEMSNL DLTKILSKKY
160 170 180 190 200
KELPEKKKMK YIQDFQREKQ EFERNLARFR EDHPDLIQNA KKSDIPEKPK
210 220 230 240 250
TPQQLWYTHE KKVYLKVRPD ATTKEVKDSL GKQWSQLSDK KTLKWIHKAL
260 270 280 290 300
EQRKEYEEIM RDYIQKHPEL NISEEGITKS TLTKAERQLK DKFDGRPTKP
310 320 330 340 350
PPNSYSLYCA ELMANMKDVP STERMVLCSQ QWKLLSQKEK DAYHKKCDQK
360 370 380 390 400
KKDYEVELLR FLESLPEEEQ QRVLGEEKML NINKKQTTSP ASKKPSQEGG
410 420 430 440 450
KGGSEKPKRP VSAMFIFSEE KRRQLQEERP ELSESELTRL LARMWNDLTE
460 470 480 490 500
KKKAKYKARE AALKAQSERK PGGEREDRGK LPESPKRAEE IWQQSVIGDY
510 520 530 540 550
LARFKNDRVK ALKAMEMTWN NMEKKEKLMW IKKAAEDQKR YERELSEMRA
560 570 580 590 600
PPAATNSSKK MKFQGEPKKP PMNGYQKFSQ ELLSNGELNH LPLKERMVEI
610 620 630 640 650
GSRWQRISQS QKEHYKKLAE EQQRQYKVHL DLWVKSLSPQ DRAAYKEYIS
660 670 680 690 700
NKRKNMTKLR GPNPKSSRTT LQSKSESEED DDEEEEDDEE EEEEEEDDEN
710 720 730 740 750
GDSSEDGGDS SESSSEDESE DGDENDDDDD DEDDEDDDDE DEDNESEGSS
760
SSSSSSGDSS DSGSN
Length:765
Mass (Da):89,509
Last modified:May 1, 1992 - v1
Checksum:i006A20A7F968DB6A
GO
Isoform UBF2 (identifier: P25976-2) [UniParc]FASTAAdd to basket
Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     221-257: Missing.

Show »
Length:728
Mass (Da):85,095
Checksum:i17108E9528846CC4
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002194221 – 257Missing in isoform UBF2. 1 PublicationAdd BLAST37

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60831 mRNA. Translation: CAA43222.1.
PIRiS22314.
UniGeneiMm.2845.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60831 mRNA. Translation: CAA43222.1.
PIRiS22314.
UniGeneiMm.2845.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V63NMR-A567-654[»]
1V64NMR-A288-382[»]
1WGFNMR-A394-470[»]
ProteinModelPortaliP25976.
SMRiP25976.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29977N.
IntActiP25976. 7 interactors.
MINTiMINT-4084181.
STRINGi10090.ENSMUSP00000078539.

PTM databases

iPTMnetiP25976.
PhosphoSitePlusiP25976.

Proteomic databases

EPDiP25976.
MaxQBiP25976.
PaxDbiP25976.
PeptideAtlasiP25976.
PRIDEiP25976.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:98512. Ubtf.

Phylogenomic databases

eggNOGiKOG0381. Eukaryota.
COG5648. LUCA.
HOGENOMiHOG000232068.
HOVERGENiHBG008708.
InParanoidiP25976.
PhylomeDBiP25976.

Miscellaneous databases

EvolutionaryTraceiP25976.
PROiP25976.
SOURCEiSearch...

Family and domain databases

Gene3Di1.10.30.10. 6 hits.
InterProiIPR029215. HMG_box_5.
IPR009071. HMG_box_dom.
[Graphical view]
PfamiPF00505. HMG_box. 3 hits.
PF09011. HMG_box_2. 1 hit.
PF14887. HMG_box_5. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 6 hits.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 6 hits.
PROSITEiPS50118. HMG_BOX_2. 6 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBF1_MOUSE
AccessioniPrimary (citable) accession number: P25976
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: November 30, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.