Nucleolar transcription factor 1 - Mus musculus (Mouse)

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Molecule processing

Regions

Amino acid modifications

Natural variations

Secondary structure

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Preferred order of sections

Molecule processing

Regions

Amino acid modifications

Natural variations

Secondary structure

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

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P25976 (UBF1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 107. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Protein names

Recommended name:
Nucleolar transcription factor 1

Alternative name(s):
Upstream-binding factor 1
Short name=UBF-1

Gene names

Name:	Ubtf
Synonyms:	Tcfubf, Ubf-1, Ubf1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Sequence length	765 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Function	Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element. Ref.4
Subunit structure	Homodimer. Binds to IRS1 and PIK3CA. Interacts with TBP. Interacts with TAF1A and TAF1D By similarity. Interacts with RASL11A. Ref.2 Ref.4
Subcellular location	Nucleus › nucleolus Ref.4.
Post-translational modification	Phosphorylated and activated by PIK3CA. Ref.3
Sequence similarities	Contains 6 HMG box DNA-binding domains.

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	DNA-binding
Molecular function	Activator
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chromatin silencing at rDNA Inferred from mutant phenotype. Source: MGI positive regulation of transcription from RNA polymerase I promoter Inferred from direct assay Ref.4. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleolus Inferred from direct assay Ref.4. Source: UniProtKB
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction Ref.4. Source: UniProtKB
Complete GO annotation...

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Chain

1 – 765

765

Nucleolar transcription factor 1

PRO_0000048626

DNA binding

112 – 180

HMG box 1

DNA binding

196 – 264

HMG box 2

DNA binding

298 – 362

HMG box 3

DNA binding

407 – 475

HMG box 4

DNA binding

482 – 549

HMG box 5

DNA binding

568 – 634

HMG box 6

Compositional bias

675 – 765

Asp/Glu/Ser-rich (acidic)

Modified residue

N-acetylmethionine By similarity

Modified residue

201

Phosphothreonine By similarity

Modified residue

273

Phosphoserine Ref.3

Modified residue

336

Phosphoserine Ref.3

Modified residue

364

Phosphoserine Ref.3

Modified residue

389

Phosphoserine Ref.3

Modified residue

412

Phosphoserine Ref.3

Modified residue

433

Phosphoserine Ref.3

Modified residue

484

Phosphoserine Ref.3

Modified residue

546

Phosphoserine Ref.3

Modified residue

584

Phosphoserine Ref.3

Modified residue

638

Phosphoserine Ref.3

Alternative sequence

221 – 257

Missing in isoform UBF2.

VSP_002194

765

Helix Strand Turn

Details...

Sequence		Length	Mass (Da)
Isoform UBF1 (Long) [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 006A20A7F968DB6A Show »	FASTA	765	89,509
10 20 30 40 50 60 MNGEADCPTD LEMAAPKGQD RWSQEDMLTL LECMKNNLPS NDSSKFKTTE SHMDWEKVAF 70 80 90 100 110 120 KDFSGDMCKL KWVEISNEVR KFRTLTELIL DAQEHVKNPY KGKKLKKHPD FPKKPLTPYF 130 140 150 160 170 180 RFFMEKRAKY AKLHPEMSNL DLTKILSKKY KELPEKKKMK YIQDFQREKQ EFERNLARFR 190 200 210 220 230 240 EDHPDLIQNA KKSDIPEKPK TPQQLWYTHE KKVYLKVRPD ATTKEVKDSL GKQWSQLSDK 250 260 270 280 290 300 KTLKWIHKAL EQRKEYEEIM RDYIQKHPEL NISEEGITKS TLTKAERQLK DKFDGRPTKP 310 320 330 340 350 360 PPNSYSLYCA ELMANMKDVP STERMVLCSQ QWKLLSQKEK DAYHKKCDQK KKDYEVELLR 370 380 390 400 410 420 FLESLPEEEQ QRVLGEEKML NINKKQTTSP ASKKPSQEGG KGGSEKPKRP VSAMFIFSEE 430 440 450 460 470 480 KRRQLQEERP ELSESELTRL LARMWNDLTE KKKAKYKARE AALKAQSERK PGGEREDRGK 490 500 510 520 530 540 LPESPKRAEE IWQQSVIGDY LARFKNDRVK ALKAMEMTWN NMEKKEKLMW IKKAAEDQKR 550 560 570 580 590 600 YERELSEMRA PPAATNSSKK MKFQGEPKKP PMNGYQKFSQ ELLSNGELNH LPLKERMVEI 610 620 630 640 650 660 GSRWQRISQS QKEHYKKLAE EQQRQYKVHL DLWVKSLSPQ DRAAYKEYIS NKRKNMTKLR 670 680 690 700 710 720 GPNPKSSRTT LQSKSESEED DDEEEEDDEE EEEEEEDDEN GDSSEDGGDS SESSSEDESE 730 740 750 760 DGDENDDDDD DEDDEDDDDE DEDNESEGSS SSSSSSGDSS DSGSN « Hide
Isoform UBF2 (Short) [UniParc]. Checksum: 17108E9528846CC4 Show »	FASTA	728	85,095
10 20 30 40 50 60 MNGEADCPTD LEMAAPKGQD RWSQEDMLTL LECMKNNLPS NDSSKFKTTE SHMDWEKVAF 70 80 90 100 110 120 KDFSGDMCKL KWVEISNEVR KFRTLTELIL DAQEHVKNPY KGKKLKKHPD FPKKPLTPYF 130 140 150 160 170 180 RFFMEKRAKY AKLHPEMSNL DLTKILSKKY KELPEKKKMK YIQDFQREKQ EFERNLARFR 190 200 210 220 230 240 EDHPDLIQNA KKSDIPEKPK TPQQLWYTHE KKVYLKVRPD EIMRDYIQKH PELNISEEGI 250 260 270 280 290 300 TKSTLTKAER QLKDKFDGRP TKPPPNSYSL YCAELMANMK DVPSTERMVL CSQQWKLLSQ 310 320 330 340 350 360 KEKDAYHKKC DQKKKDYEVE LLRFLESLPE EEQQRVLGEE KMLNINKKQT TSPASKKPSQ 370 380 390 400 410 420 EGGKGGSEKP KRPVSAMFIF SEEKRRQLQE ERPELSESEL TRLLARMWND LTEKKKAKYK 430 440 450 460 470 480 AREAALKAQS ERKPGGERED RGKLPESPKR AEEIWQQSVI GDYLARFKND RVKALKAMEM 490 500 510 520 530 540 TWNNMEKKEK LMWIKKAAED QKRYERELSE MRAPPAATNS SKKMKFQGEP KKPPMNGYQK 550 560 570 580 590 600 FSQELLSNGE LNHLPLKERM VEIGSRWQRI SQSQKEHYKK LAEEQQRQYK VHLDLWVKSL 610 620 630 640 650 660 SPQDRAAYKE YISNKRKNMT KLRGPNPKSS RTTLQSKSES EEDDDEEEED DEEEEEEEED 670 680 690 700 710 720 DENGDSSEDG GDSSESSSED ESEDGDENDD DDDDEDDEDD DDEDEDNESE GSSSSSSSSG DSSDSGSN « Hide

[1]	"Cloning and structural analysis of cDNA and the gene for mouse transcription factor UBF." Hisatake K., Nishimura T., Maeda Y., Hanada K., Song C.Z., Muramatsu M. Nucleic Acids Res. 19:4631-4637(1991) [PubMed: 1891354] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS UBF1 AND UBF2). Strain: C3H/He.
[2]	"Control of cell size through phosphorylation of upstream binding factor 1 by nuclear phosphatidylinositol 3-kinase." Drakas R., Tu X., Baserga R. Proc. Natl. Acad. Sci. U.S.A. 101:9272-9276(2004) [PubMed: 15197263] [Abstract] Cited for: INTERACTION WITH IRS1 AND PIK3CA.
[3]	"Mass spectrometric identification of phosphorylation sites of rRNA transcription factor upstream binding factor." Lin C.H., Platt M.D., Ficarro S.B., Hoofnagle M.H., Shabanowitz J., Comai L., Hunt D.F., Owens G.K. Am. J. Physiol. 292:C1617-C1624(2007) [PubMed: 17182730] [Abstract] Cited for: PHOSPHORYLATION AT SER-273; SER-336; SER-364; SER-389; SER-412; SER-433; SER-484; SER-546; SER-584 AND SER-638.
[4]	"Chromatin association and regulation of rDNA transcription by the Ras-family protein RasL11a." Pistoni M., Verrecchia A., Doni M., Guccione E., Amati B. EMBO J. 29:1215-1224(2010) [PubMed: 20168301] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH RASL11A.
[5]	"Solution structure of the 3rd, 4th and 6th HMG-box of mouse UBF1." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 288-654.
+	Additional computationally mapped references.

EMBL
GenBank
DDBJ

X60831 mRNA. Translation: CAA43222.1.

IPI

IPI00114869.
IPI00223407.

PIR

S22314.

UniGene

Mm.2845.

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1V63	NMR	-	A	567-654	[»]
1V64	NMR	-	A	288-382	[»]
1WGF	NMR	-	A	394-470	[»]

ProteinModelPortal

P25976.

SMR

P25976. Positions 103-271, 288-476, 481-546, 567-655.

ModBase

Search...

DIP

DIP-29977N.

MINT

MINT-4084181.

STRING

P25976.

PhosphoSite

P25976.

PRIDE

P25976.

StructuralBiologyKnowledgebase

Search...

MGI

MGI:98512. Ubtf.

GeneTree

ENSGT00530000063934.

HOGENOM

HBG717198.

HOVERGEN

HBG008708.

InParanoid

P25976.

PhylomeDB

P25976.

ArrayExpress

P25976.

Bgee

P25976.

Genevestigator

P25976.

GermOnline

ENSMUSG00000020923. Mus musculus.

InterPro

IPR000910. HMG_HMG1/HMG2.
IPR009071. HMG_superfamily.
[Graphical view]

Gene3D

G3DSA:1.10.30.10. HMG-box. 6 hits.

Pfam

PF00505. HMG_box. 4 hits.
[Graphical view]

SMART

SM00398. HMG. 6 hits.
[Graphical view]

SUPFAM

SSF47095. HMG-box. 6 hits.

PROSITE

PS50118. HMG_BOX_2. 6 hits.
[Graphical view]

ProtoNet

Search...

SOURCE

Search...

Entry name

UBF1_MOUSE

Accession

Primary (citable) accession number: P25976

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	November 16, 2011
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform UBF1 (identifier: P25976-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform UBF2 (identifier: P25976-2)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
221-257: Missing.