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Reviewed, UniProtKB/Swiss-Prot P25976 (UBF1_MOUSE)

Last modified February 9, 2010. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Nucleolar transcription factor 1
Alternative name(s):
    Upstream-binding factor 1
      Short name=UBF-1
Gene names
Name: Ubtf
Synonyms: Tcfubf, Ubf-1, Ubf1
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length765 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.

Subunit structure

Homodimer. Binds to IRS1 and PIK3CA. Interacts with TBP. Interacts with TAF1A and TAF1D By similarity. Ref.2

Subcellular location

Nucleus.

Post-translational modification

Phosphorylated and activated by PIK3CA. Ref.3

Sequence similarities

Contains 6 HMG box DNA-binding domains.

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Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainRepeat
   LigandDNA-binding
   Molecular functionActivator
   PTMAcetylation
Phosphoprotein
   Technical term3D-structure
Gene Ontology (GO)
   Biological processchromatin silencing at rDNA

Inferred from mutant phenotype. Source: MGI

transcription

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentnucleolus

Inferred from direct assay. Source: MGI

   Molecular functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform UBF1 (identifier: P25976-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform UBF2 (identifier: P25976-2)

Also known as: Short;

The sequence of this isoform differs from the canonical sequence as follows:
     221-257: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 765765Nucleolar transcription factor 1
PRO_0000048626

Regions

DNA binding112 – 18069HMG box 1
DNA binding196 – 26469HMG box 2
DNA binding298 – 36265HMG box 3
DNA binding407 – 47569HMG box 4
DNA binding482 – 54968HMG box 5
DNA binding568 – 63467HMG box 6
Compositional bias675 – 76591Asp/Glu/Ser-rich (acidic)

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue2011Phosphothreonine By similarity
Modified residue2731Phosphoserine Ref.3
Modified residue3361Phosphoserine Ref.3
Modified residue3641Phosphoserine Ref.3
Modified residue3891Phosphoserine Ref.3
Modified residue4121Phosphoserine Ref.3
Modified residue4331Phosphoserine Ref.3
Modified residue4841Phosphoserine Ref.3
Modified residue5461Phosphoserine Ref.3
Modified residue5841Phosphoserine Ref.3
Modified residue6381Phosphoserine Ref.3

Natural variations

Alternative sequence221 – 25737Missing in isoform UBF2.
VSP_002194

Secondary structure

1 ................................ 765
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Isoform UBF1 (Long) [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 006A20A7F968DB6A

FASTA76589,509
        10         20         30         40         50         60 
MNGEADCPTD LEMAAPKGQD RWSQEDMLTL LECMKNNLPS NDSSKFKTTE SHMDWEKVAF 

        70         80         90        100        110        120 
KDFSGDMCKL KWVEISNEVR KFRTLTELIL DAQEHVKNPY KGKKLKKHPD FPKKPLTPYF 

       130        140        150        160        170        180 
RFFMEKRAKY AKLHPEMSNL DLTKILSKKY KELPEKKKMK YIQDFQREKQ EFERNLARFR 

       190        200        210        220        230        240 
EDHPDLIQNA KKSDIPEKPK TPQQLWYTHE KKVYLKVRPD ATTKEVKDSL GKQWSQLSDK 

       250        260        270        280        290        300 
KTLKWIHKAL EQRKEYEEIM RDYIQKHPEL NISEEGITKS TLTKAERQLK DKFDGRPTKP 

       310        320        330        340        350        360 
PPNSYSLYCA ELMANMKDVP STERMVLCSQ QWKLLSQKEK DAYHKKCDQK KKDYEVELLR 

       370        380        390        400        410        420 
FLESLPEEEQ QRVLGEEKML NINKKQTTSP ASKKPSQEGG KGGSEKPKRP VSAMFIFSEE 

       430        440        450        460        470        480 
KRRQLQEERP ELSESELTRL LARMWNDLTE KKKAKYKARE AALKAQSERK PGGEREDRGK 

       490        500        510        520        530        540 
LPESPKRAEE IWQQSVIGDY LARFKNDRVK ALKAMEMTWN NMEKKEKLMW IKKAAEDQKR 

       550        560        570        580        590        600 
YERELSEMRA PPAATNSSKK MKFQGEPKKP PMNGYQKFSQ ELLSNGELNH LPLKERMVEI 

       610        620        630        640        650        660 
GSRWQRISQS QKEHYKKLAE EQQRQYKVHL DLWVKSLSPQ DRAAYKEYIS NKRKNMTKLR 

       670        680        690        700        710        720 
GPNPKSSRTT LQSKSESEED DDEEEEDDEE EEEEEEDDEN GDSSEDGGDS SESSSEDESE 

       730        740        750        760 
DGDENDDDDD DEDDEDDDDE DEDNESEGSS SSSSSSGDSS DSGSN

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Isoform UBF2 (Short).

Checksum: 17108E9528846CC4
Show »

FASTA72885,095

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References

[1]"Cloning and structural analysis of cDNA and the gene for mouse transcription factor UBF."
Hisatake K., Nishimura T., Maeda Y., Hanada K., Song C.Z., Muramatsu M.
Nucleic Acids Res. 19:4631-4637(1991) [PubMed: 1891354] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS UBF1 AND UBF2).
Strain: C3H/He.
[2]"Control of cell size through phosphorylation of upstream binding factor 1 by nuclear phosphatidylinositol 3-kinase."
Drakas R., Tu X., Baserga R.
Proc. Natl. Acad. Sci. U.S.A. 101:9272-9276(2004) [PubMed: 15197263] [Abstract]
Cited for: INTERACTION WITH IRS1 AND PIK3CA.
[3]"Mass spectrometric identification of phosphorylation sites of rRNA transcription factor upstream binding factor."
Lin C.H., Platt M.D., Ficarro S.B., Hoofnagle M.H., Shabanowitz J., Comai L., Hunt D.F., Owens G.K.
Am. J. Physiol. 292:C1617-C1624(2007) [PubMed: 17182730] [Abstract]
Cited for: PHOSPHORYLATION AT SER-273; SER-336; SER-364; SER-389; SER-412; SER-433; SER-484; SER-546; SER-584 AND SER-638.
[4]"Solution structure of the 3rd, 4th and 6th HMG-box of mouse UBF1."
RIKEN structural genomics initiative (RSGI)
Submitted (NOV-2004) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 288-654.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X60831 mRNA. Translation: CAA43222.1.
IPIIPI00114869.
IPI00223407.
PIRS22314.
UniGeneMm.2845

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1V63NMR-A567-654[»]
1V64NMR-A288-382[»]
1WGFNMR-A394-470[»]
SMRP25976. Positions 103-192, 191-272, 481-546.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-29977N.
STRINGP25976.

PTM databases

PhosphoSiteP25976.

Proteomic databases

PRIDEP25976.

Genome annotation databases

EnsemblENSMUST00000079589; ENSMUSP00000078539; ENSMUSG00000020923; Mus musculus. [Genome view]
ENSMUST00000107119; ENSMUSP00000102736; ENSMUSG00000020923; Mus musculus. [Genome view]
ENSMUST00000107121; ENSMUSP00000102738; ENSMUSG00000020923; Mus musculus. [Genome view]
ENSMUST00000107123; ENSMUSP00000102740; ENSMUSG00000020923; Mus musculus. [Genome view]
UCSCuc007lri.1. mouse.
uc007lrk.1. mouse.

Organism-specific databases

MGIMGI:98512. Ubtf.

Phylogenomic databases

HOGENOMHBG717198.
HOVERGENP25976.
InParanoidP25976.
PhylomeDBP25976.

Gene expression databases

ArrayExpressP25976.
BgeeP25976.
GenevestigatorP25976.
GermOnlineENSMUSG00000020923. Mus musculus.

Family and domain databases

InterProIPR000910. HMG_HMG1/HMG2.
IPR009071. HMG_superfamily.
[Graphical view]
Gene3DG3DSA:1.10.30.10. HMG-box. 5 hits.
PfamPF00505. HMG_box. 4 hits.
[Graphical view]
SMARTSM00398. HMG. 6 hits.
[Graphical view]
PROSITEPS50118. HMG_BOX_2. 6 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

SOURCESearch...

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Entry information

Entry nameUBF1_MOUSE
AccessionPrimary (citable) accession number: P25976
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: February 9, 2010
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents