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Protein

Cathepsin L1

Gene

CTSL

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Important for the overall degradation of proteins in lysosomes.

Catalytic activityi

Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei138By similarity1
Active sitei277By similarity1
Active sitei301By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease

Enzyme and pathway databases

ReactomeiR-BTA-1474228 Degradation of the extracellular matrix
R-BTA-1592389 Activation of Matrix Metalloproteinases
R-BTA-1679131 Trafficking and processing of endosomal TLR
R-BTA-2022090 Assembly of collagen fibrils and other multimeric structures
R-BTA-2132295 MHC class II antigen presentation
R-BTA-8939242 RUNX1 regulates transcription of genes involved in differentiation of keratinocytes

Protein family/group databases

MEROPSiI29.010

Names & Taxonomyi

Protein namesi
Recommended name:
Cathepsin L1 (EC:3.4.22.15)
Alternative name(s):
Cathepsin L
Cleaved into the following 2 chains:
Gene namesi
Name:CTSL
Synonyms:CTSL1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2113

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17By similarityAdd BLAST17
PropeptideiPRO_000002623618 – 117Activation peptideAdd BLAST100
ChainiPRO_0000026237118 – 289Cathepsin L1 heavy chainAdd BLAST172
PropeptideiPRO_0000026238290 – 2912
ChainiPRO_0000026239292 – 334Cathepsin L1 light chainAdd BLAST43

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi135 ↔ 178By similarity
Disulfide bondi169 ↔ 212By similarity
Glycosylationi222N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi270 ↔ 323Interchain (between heavy and light chains)By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP25975
PeptideAtlasiP25975
PRIDEiP25975

Expressioni

Gene expression databases

BgeeiENSBTAG00000017077
ExpressionAtlasiP25975 baseline and differential

Interactioni

Subunit structurei

Dimer of a heavy and a light chain linked by disulfide bonds.

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000022710

Chemistry databases

BindingDBiP25975

Structurei

3D structure databases

ProteinModelPortaliP25975
SMRiP25975
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1543 Eukaryota
COG4870 LUCA
GeneTreeiENSGT00900000140823
HOGENOMiHOG000230774
HOVERGENiHBG011513
InParanoidiP25975
KOiK01365
OMAiQHYRKSG
OrthoDBiEOG091G0AKT
TreeFamiTF313739

Family and domain databases

InterProiView protein in InterPro
IPR025661 Pept_asp_AS
IPR000169 Pept_cys_AS
IPR025660 Pept_his_AS
IPR013128 Peptidase_C1A
IPR000668 Peptidase_C1A_C
IPR013201 Prot_inhib_I29
PANTHERiPTHR12411 PTHR12411, 1 hit
PfamiView protein in Pfam
PF08246 Inhibitor_I29, 1 hit
PF00112 Peptidase_C1, 1 hit
PRINTSiPR00705 PAPAIN
SMARTiView protein in SMART
SM00848 Inhibitor_I29, 1 hit
SM00645 Pept_C1, 1 hit
PROSITEiView protein in PROSITE
PS00640 THIOL_PROTEASE_ASN, 1 hit
PS00139 THIOL_PROTEASE_CYS, 1 hit
PS00639 THIOL_PROTEASE_HIS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25975-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPSFFLTVL CLGVASAAPK LDPNLDAHWH QWKATHRRLY GMNEEEWRRA
60 70 80 90 100
VWEKNKKIID LHNQEYSEGK HGFRMAMNAF GDMTNEEFRQ VMNGFQNQKH
110 120 130 140 150
KKGKLFHEPL LVDVPKSVDW TKKGYVTPVK NQGQCGSCWA FSATGALEGQ
160 170 180 190 200
MFRKTGKLVS LSEQNLVDCS RAQGNQGCNG GLMDNAFQYI KDNGGLDSEE
210 220 230 240 250
SYPYLATDTN SCNYKPECSA ANDTGFVDIP QREKALMKAV ATVGPISVAI
260 270 280 290 300
DAGHTSFQFY KSGIYYDPDC SSKDLDHGVL VVGYGFEGTD SNNNKFWIVK
310 320 330
NSWGPEWGWN GYVKMAKDQN NHCGIATAAS YPTV
Length:334
Mass (Da):37,347
Last modified:May 30, 2006 - v3
Checksum:iECA5D7415DDECFC9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72G → A in CAA62870 (Ref. 1) Curated1
Sequence conflicti116K → W AA sequence (PubMed:2044774).Curated1
Sequence conflicti116K → W AA sequence (PubMed:1515067).Curated1
Sequence conflicti272S → C in CAA62870 (Ref. 1) Curated1
Sequence conflicti305P → G AA sequence (PubMed:2044774).Curated1
Sequence conflicti305P → G AA sequence (PubMed:1515067).Curated1
Sequence conflicti310N → G AA sequence (PubMed:2044774).Curated1
Sequence conflicti310N → G AA sequence (PubMed:1515067).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91755 mRNA Translation: CAA62870.1
BC102312 mRNA Translation: AAI02313.1
AB017648 Genomic DNA Translation: BAA33398.1
PIRiS15845
RefSeqiNP_776457.1, NM_174032.2
UniGeneiBt.3987

Genome annotation databases

EnsembliENSBTAT00000022710; ENSBTAP00000022710; ENSBTAG00000017077
ENSBTAT00000036427; ENSBTAP00000036285; ENSBTAG00000017077
GeneIDi281108
KEGGibta:281108

Similar proteinsi

Entry informationi

Entry nameiCATL1_BOVIN
AccessioniPrimary (citable) accession number: P25975
Secondary accession number(s): O77502, Q3T0P2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 30, 2006
Last modified: March 28, 2018
This is version 149 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome