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Protein

Cathepsin L1

Gene

CTSL

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Important for the overall degradation of proteins in lysosomes.

Catalytic activityi

Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei138By similarity1
Active sitei277By similarity1
Active sitei301By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease

Enzyme and pathway databases

ReactomeiR-BTA-1474228. Degradation of the extracellular matrix.
R-BTA-1592389. Activation of Matrix Metalloproteinases.
R-BTA-2022090. Assembly of collagen fibrils and other multimeric structures.
R-BTA-2132295. MHC class II antigen presentation.
R-BTA-8939242. RUNX1 regulates transcription of genes involved in differentiation of keratinocytes.

Protein family/group databases

MEROPSiI29.010.

Names & Taxonomyi

Protein namesi
Recommended name:
Cathepsin L1 (EC:3.4.22.15)
Alternative name(s):
Cathepsin L
Cleaved into the following 2 chains:
Gene namesi
Name:CTSL
Synonyms:CTSL1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2113.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17By similarityAdd BLAST17
PropeptideiPRO_000002623618 – 117Activation peptideAdd BLAST100
ChainiPRO_0000026237118 – 289Cathepsin L1 heavy chainAdd BLAST172
PropeptideiPRO_0000026238290 – 2912
ChainiPRO_0000026239292 – 334Cathepsin L1 light chainAdd BLAST43

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi135 ↔ 178By similarity
Disulfide bondi169 ↔ 212By similarity
Glycosylationi222N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi270 ↔ 323Interchain (between heavy and light chains)By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP25975.
PeptideAtlasiP25975.
PRIDEiP25975.

Expressioni

Gene expression databases

BgeeiENSBTAG00000017077.
ExpressionAtlasiP25975. baseline and differential.

Interactioni

Subunit structurei

Dimer of a heavy and a light chain linked by disulfide bonds.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000022710.

Chemistry databases

BindingDBiP25975.

Structurei

3D structure databases

ProteinModelPortaliP25975.
SMRiP25975.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1543. Eukaryota.
COG4870. LUCA.
GeneTreeiENSGT00760000118871.
HOGENOMiHOG000230774.
HOVERGENiHBG011513.
InParanoidiP25975.
KOiK01365.
OMAiFRYIKDN.
OrthoDBiEOG091G0AKT.
TreeFamiTF313739.

Family and domain databases

InterProiView protein in InterPro
IPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiView protein in Pfam
PF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
PRINTSiPR00705. PAPAIN.
SMARTiView protein in SMART
SM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
PROSITEiView protein in PROSITE
PS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25975-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPSFFLTVL CLGVASAAPK LDPNLDAHWH QWKATHRRLY GMNEEEWRRA
60 70 80 90 100
VWEKNKKIID LHNQEYSEGK HGFRMAMNAF GDMTNEEFRQ VMNGFQNQKH
110 120 130 140 150
KKGKLFHEPL LVDVPKSVDW TKKGYVTPVK NQGQCGSCWA FSATGALEGQ
160 170 180 190 200
MFRKTGKLVS LSEQNLVDCS RAQGNQGCNG GLMDNAFQYI KDNGGLDSEE
210 220 230 240 250
SYPYLATDTN SCNYKPECSA ANDTGFVDIP QREKALMKAV ATVGPISVAI
260 270 280 290 300
DAGHTSFQFY KSGIYYDPDC SSKDLDHGVL VVGYGFEGTD SNNNKFWIVK
310 320 330
NSWGPEWGWN GYVKMAKDQN NHCGIATAAS YPTV
Length:334
Mass (Da):37,347
Last modified:May 30, 2006 - v3
Checksum:iECA5D7415DDECFC9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72G → A in CAA62870 (Ref. 1) Curated1
Sequence conflicti116K → W AA sequence (PubMed:2044774).Curated1
Sequence conflicti116K → W AA sequence (PubMed:1515067).Curated1
Sequence conflicti272S → C in CAA62870 (Ref. 1) Curated1
Sequence conflicti305P → G AA sequence (PubMed:2044774).Curated1
Sequence conflicti305P → G AA sequence (PubMed:1515067).Curated1
Sequence conflicti310N → G AA sequence (PubMed:2044774).Curated1
Sequence conflicti310N → G AA sequence (PubMed:1515067).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91755 mRNA. Translation: CAA62870.1.
BC102312 mRNA. Translation: AAI02313.1.
AB017648 Genomic DNA. Translation: BAA33398.1.
PIRiS15845.
RefSeqiNP_776457.1. NM_174032.2.
UniGeneiBt.3987.

Genome annotation databases

EnsembliENSBTAT00000022710; ENSBTAP00000022710; ENSBTAG00000017077.
ENSBTAT00000036427; ENSBTAP00000036285; ENSBTAG00000017077.
GeneIDi281108.
KEGGibta:281108.

Similar proteinsi

Entry informationi

Entry nameiCATL1_BOVIN
AccessioniPrimary (citable) accession number: P25975
Secondary accession number(s): O77502, Q3T0P2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 30, 2006
Last modified: September 27, 2017
This is version 145 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families