Reviewed, UniProtKB/Swiss-Prot P25975 (CATL1_BOVIN)

Names and origin

Protein names

Recommended name:
    Cathepsin L1
    EC=3.4.22.15
Cleaved into the following 2 chains:
    1- Recommended name:
            Cathepsin L1 heavy chain
    2- Recommended name:
            Cathepsin L1 light chain

Gene names

Name:	CTSL1
Synonyms:	CTSL

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Important for the overall degradation of proteins in lysosomes.
Catalytic activity	Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.
Subunit structure	Dimer of a heavy and a light chain linked by disulfide bonds.
Subcellular location	Lysosome.
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Propeptide	18 – 117	100	Activation peptide		PRO_0000026236
	Chain	118 – 289	172	Cathepsin L1 heavy chain		PRO_0000026237
	Propeptide	290 – 291	2			PRO_0000026238
	Chain	292 – 334	43	Cathepsin L1 light chain		PRO_0000026239
Sites
	Active site	138	1	By similarity
	Active site	277	1	By similarity
	Active site	301	1	By similarity
Amino acid modifications
	Glycosylation	222	1	N-linked (GlcNAc...) Potential
	Disulfide bond	135 ↔ 178		By similarity
	Disulfide bond	169 ↔ 212		By similarity
	Disulfide bond	270 ↔ 323		Interchain (between heavy and light chains) By similarity
Experimental info
	Sequence conflict	72	1	G → A in CAA62870. Ref.1
	Sequence conflict	116	1	K → W Ref.4
	Sequence conflict	116	1	K → W Ref.5
	Sequence conflict	272	1	S → C in CAA62870. Ref.1
	Sequence conflict	305	1	P → G Ref.4
	Sequence conflict	305	1	P → G Ref.5
	Sequence conflict	310	1	N → G Ref.4
	Sequence conflict	310	1	N → G Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P25975-1 [UniParc]. Last modified May 30, 2006. Version 3. Checksum: ECA5D7415DDECFC9	FASTA	334	37,347
	10 20 30 40 50 60 MNPSFFLTVL CLGVASAAPK LDPNLDAHWH QWKATHRRLY GMNEEEWRRA VWEKNKKIID 70 80 90 100 110 120 LHNQEYSEGK HGFRMAMNAF GDMTNEEFRQ VMNGFQNQKH KKGKLFHEPL LVDVPKSVDW 130 140 150 160 170 180 TKKGYVTPVK NQGQCGSCWA FSATGALEGQ MFRKTGKLVS LSEQNLVDCS RAQGNQGCNG 190 200 210 220 230 240 GLMDNAFQYI KDNGGLDSEE SYPYLATDTN SCNYKPECSA ANDTGFVDIP QREKALMKAV 250 260 270 280 290 300 ATVGPISVAI DAGHTSFQFY KSGIYYDPDC SSKDLDHGVL VVGYGFEGTD SNNNKFWIVK 310 320 330 NSWGPEWGWN GYVKMAKDQN NHCGIATAAS YPTV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Voelkel H., Schultz J.E., Kurz U. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retinal pigment epithelium.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.
[3]	"Bos taurus cathepsin L gene." Miyashita N. Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-301.
[4]	"The complete amino acid sequence of bovine cathepsin S and a partial sequence of bovine cathepsin L." Ritonja A., Colic A., Dolenc I., Ogrinc T., Podobnik M., Turk V. FEBS Lett. 283:329-331(1991) [PubMed: 2044774] [Abstract] Cited for: PROTEIN SEQUENCE OF 114-132 AND 293-311.
[5]	"Bovine cathepsins S and L: isolation and amino acid sequences." Dolenc I., Ritonja A., Colic A., Podobnik M., Ogrinc T., Turk V. Biol. Chem. Hoppe-Seyler 373:407-412(1992) [PubMed: 1515067] [Abstract] Cited for: PROTEIN SEQUENCE OF 114-132 AND 293-311. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X91755 mRNA. Translation: CAA62870.1. BC102312 mRNA. Translation: AAI02313.1. AB017648 Genomic DNA. Translation: BAA33398.1.
IPI	IPI00687440.
PIR	S15845.
RefSeq	NP_776457.1.
UniGene	Bt.3987
3D structure databases
HSSP	HSSP built from PDB template 1FH0 based on UniProtKB O60911.
SMR	P25975. Positions 21-334.
ModBase	Search...
Protein-protein interaction databases
STRING	P25975.
Genome annotation databases
Ensembl	ENSBTAT00000022710; ENSBTAP00000022710; ENSBTAG00000017077; Bos taurus. [Genome view] ENSBTAT00000036427; ENSBTAP00000036285; ENSBTAG00000017077; Bos taurus. [Genome view]
GeneID	281108.
KEGG	bta:281108.
Organism-specific databases
CTD	281108.
Phylogenomic databases
HOVERGEN	P25975.
OMA	RMERNIK.
Enzyme and pathway databases
BRENDA	3.4.22.15. 251.
Family and domain databases
InterPro	IPR000169. Pept_cys_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. IPR013201. Prot_inhib_I29. [Graphical view]
PANTHER	PTHR12411. Peptidase_C1A. 1 hit.
Pfam	PF08246. Inhibitor_I29. 1 hit. PF00112. Peptidase_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
ProDom	PD000158. Peptidase_C1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. 1 hit. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
BindingDB	P25975.

Entry information

Entry name

CATL1_BOVIN

Accession

Primary (citable) accession number: P25975
Secondary accession number(s): O77502, Q3T0P2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 30, 2006
Last modified:	November 3, 2009
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)