Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P25971

- PYRF_BACSU

UniProt

P25971 - PYRF_BACSU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Orotidine 5'-phosphate decarboxylase

Gene

pyrF

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP).

Catalytic activityi

Orotidine 5'-phosphate = UMP + CO2.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111Substrate
Binding sitei33 – 331Substrate
Active sitei62 – 621Proton donor
Binding sitei123 – 1231Substrate
Binding sitei185 – 1851Substrate
Binding sitei194 – 1941Substrate
Binding sitei214 – 2141Substrate; via amide nitrogen
Binding sitei215 – 2151Substrate

GO - Molecular functioni

  1. orotidine-5'-phosphate decarboxylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' pyrimidine nucleobase biosynthetic process Source: InterPro
  2. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Pyrimidine biosynthesis

Enzyme and pathway databases

BioCyciBSUB:BSU15550-MONOMER.
UniPathwayiUPA00070; UER00120.

Names & Taxonomyi

Protein namesi
Recommended name:
Orotidine 5'-phosphate decarboxylase (EC:4.1.1.23)
Alternative name(s):
OMP decarboxylase
Short name:
OMPDCase
Short name:
OMPdecase
Gene namesi
Name:pyrF
Ordered Locus Names:BSU15550
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU15550. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 239239Orotidine 5'-phosphate decarboxylasePRO_0000134527Add
BLAST

Proteomic databases

PaxDbiP25971.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

IntActiP25971. 1 interaction.
MINTiMINT-8366633.
STRINGi224308.BSU15550.

Structurei

Secondary structure

1
239
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 93
Helixi15 – 217
Helixi23 – 253
Beta strandi31 – 344
Helixi36 – 427
Helixi44 – 529
Beta strandi56 – 638
Helixi67 – 7812
Turni79 – 813
Beta strandi83 – 886
Helixi89 – 913
Helixi93 – 10614
Beta strandi115 – 1195
Helixi127 – 1326
Helixi140 – 15314
Beta strandi157 – 1604
Helixi163 – 1653
Helixi166 – 1694
Turni170 – 1723
Beta strandi178 – 1814
Helixi200 – 2056
Beta strandi209 – 2135
Helixi215 – 2184
Helixi223 – 23513

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DBTX-ray2.40A/B/C1-239[»]
ProteinModelPortaliP25971.
SMRiP25971. Positions 1-237.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25971.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni60 – 6910Substrate binding

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0284.
HOGENOMiHOG000226071.
InParanoidiP25971.
KOiK01591.
OMAiRPITQSA.
OrthoDBiEOG6N6815.
PhylomeDBiP25971.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01200_B. OMPdecase_type1_B.
InterProiIPR013785. Aldolase_TIM.
IPR014732. OMPdecase.
IPR018089. OMPdecase_AS.
IPR001754. OMPdeCOase_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00215. OMPdecase. 1 hit.
[Graphical view]
SMARTiSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR01740. pyrF. 1 hit.
PROSITEiPS00156. OMPDECASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25971-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKNNLPIIAL DFASAEETLA FLAPFQQEPL FVKVGMELFY QEGPSIVKQL
60 70 80 90 100
KERNCELFLD LKLHDIPTTV NKAMKRLASL GVDLVNVHAA GGKKMMQAAL
110 120 130 140 150
EGLEEGTPAG KKRPSLIAVT QLTSTSEQIM KDELLIEKSL IDTVVHYSKQ
160 170 180 190 200
AEESGLDGVV CSVHEAKAIY QAVSPSFLTV TPGIRMSEDA ANDQVRVATP
210 220 230
AIAREKGSSA IVVGRSITKA EDPVKAYKAV RLEWEGIKS
Length:239
Mass (Da):25,992
Last modified:May 1, 1992 - v1
Checksum:iDB1743714ED052E7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59757 Genomic DNA. Translation: AAA21273.1.
AL009126 Genomic DNA. Translation: CAB13429.1.
PIRiI39845.
RefSeqiNP_389438.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB13429; CAB13429; BSU15550.
GeneIDi935960.
KEGGibsu:BSU15550.
PATRICi18974917. VBIBacSub10457_1650.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59757 Genomic DNA. Translation: AAA21273.1 .
AL009126 Genomic DNA. Translation: CAB13429.1 .
PIRi I39845.
RefSeqi NP_389438.1. NC_000964.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1DBT X-ray 2.40 A/B/C 1-239 [» ]
ProteinModelPortali P25971.
SMRi P25971. Positions 1-237.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P25971. 1 interaction.
MINTi MINT-8366633.
STRINGi 224308.BSU15550.

Proteomic databases

PaxDbi P25971.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB13429 ; CAB13429 ; BSU15550 .
GeneIDi 935960.
KEGGi bsu:BSU15550.
PATRICi 18974917. VBIBacSub10457_1650.

Organism-specific databases

GenoListi BSU15550. [Micado ]

Phylogenomic databases

eggNOGi COG0284.
HOGENOMi HOG000226071.
InParanoidi P25971.
KOi K01591.
OMAi RPITQSA.
OrthoDBi EOG6N6815.
PhylomeDBi P25971.

Enzyme and pathway databases

UniPathwayi UPA00070 ; UER00120 .
BioCyci BSUB:BSU15550-MONOMER.

Miscellaneous databases

EvolutionaryTracei P25971.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01200_B. OMPdecase_type1_B.
InterProi IPR013785. Aldolase_TIM.
IPR014732. OMPdecase.
IPR018089. OMPdecase_AS.
IPR001754. OMPdeCOase_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view ]
Pfami PF00215. OMPdecase. 1 hit.
[Graphical view ]
SMARTi SM00934. OMPdecase. 1 hit.
[Graphical view ]
SUPFAMi SSF51366. SSF51366. 1 hit.
TIGRFAMsi TIGR01740. pyrF. 1 hit.
PROSITEi PS00156. OMPDECASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Functional organization and nucleotide sequence of the Bacillus subtilis pyrimidine biosynthetic operon."
    Quinn C.L., Stephenson B.T., Switzer R.L.
    J. Biol. Chem. 266:9113-9127(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "The crystal structure and mechanism of orotidine 5'-monophosphate decarboxylase."
    Appleby T.C., Kinsland C., Begley T.P., Ealick S.E.
    Proc. Natl. Acad. Sci. U.S.A. 97:2005-2010(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) IN COMPLEX WITH UMP, SUBUNIT.

Entry informationi

Entry nameiPYRF_BACSU
AccessioniPrimary (citable) accession number: P25971
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: October 29, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3