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Protein

NF-kappa-B inhibitor alpha

Gene

NFKBIA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.1 Publication

GO - Molecular functioni

  • enzyme binding Source: UniProtKB
  • heat shock protein binding Source: Ensembl
  • identical protein binding Source: IntAct
  • NF-kappaB binding Source: MGI
  • nuclear localization sequence binding Source: UniProtKB
  • transcription factor binding Source: ProtInc
  • ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processHost-virus interaction

Enzyme and pathway databases

ReactomeiR-HSA-1169091 Activation of NF-kappaB in B cells
R-HSA-1810476 RIP-mediated NFkB activation via ZBP1
R-HSA-202424 Downstream TCR signaling
R-HSA-209560 NF-kB is activated and signals survival
R-HSA-2871837 FCERI mediated NF-kB activation
R-HSA-445989 TAK1 activates NFkB by phosphorylation and activation of IKKs complex
R-HSA-5603027 IKBKG deficiency causes anhidrotic ectodermal dysplasia with immunodeficiency (EDA-ID) (via TLR)
R-HSA-5603029 IkBA variant leads to EDA-ID
R-HSA-5607764 CLEC7A (Dectin-1) signaling
R-HSA-5689880 Ub-specific processing proteases
R-HSA-9020702 Interleukin-1 signaling
R-HSA-933542 TRAF6 mediated NF-kB activation
SABIO-RKiP25963
SignaLinkiP25963
SIGNORiP25963

Names & Taxonomyi

Protein namesi
Recommended name:
NF-kappa-B inhibitor alpha
Alternative name(s):
I-kappa-B-alpha
Short name:
IkB-alpha
Short name:
IkappaBalpha
Major histocompatibility complex enhancer-binding protein MAD3
Gene namesi
Name:NFKBIA
Synonyms:IKBA, MAD3, NFKBI
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000100906.10
HGNCiHGNC:7797 NFKBIA
MIMi164008 gene
neXtProtiNX_P25963

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant (ADEDAID)2 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA form of ectoderma dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. This form of ectodermal dysplasia is associated with decreased production of pro-inflammatory cytokines and certain interferons, rendering patients susceptible to infection.
See also OMIM:612132
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03487132S → I in ADEDAID. 1 PublicationCorresponds to variant dbSNP:rs28933100Ensembl.1

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi21K → R: Little change in Tax-stimulated transactivation. No sumoylation. Greatly reduced Tax- or cytokine-stimulated transactivation and decrease in ubiquitination and degradation; when associated with R-22. 4 Publications1
Mutagenesisi22K → R: Little change in Tax-stimulated transactivation. No sumoylation. Greatly reduced Tax- or cytokine-stimulated transactivation and decrease in ubiquitination and degradation; when associated with R-21. 4 Publications1
Mutagenesisi31D → A: Loss of phosphorylation; when associated with A-35. 1 Publication1
Mutagenesisi32S → A: Loss of phosphorylation, ubiquitination and degradation; when associated with A-36. 2 Publications1
Mutagenesisi32S → T: Decrease in phosphorylation and degradation; when associated with T-36. 2 Publications1
Mutagenesisi35D → A: Loss in phosphorylation; when associated with A-31. 1 Publication1
Mutagenesisi35D → G: No change neither in phosphorylation, nor on degradation. 1 Publication1
Mutagenesisi36S → A: Loss of phosphorylation, ubiquitination, and degradation; when associated with A-32. 2 Publications1
Mutagenesisi36S → T: Decrease in phosphorylation and degradation; when associated with T-32. 2 Publications1
Mutagenesisi38K → R: No change in Tax-stimulated transactivation. No change in Tax-stimulated transactivation; when associated with R-47. 1 Publication1
Mutagenesisi42Y → F: No phosphorylation. 1 Publication1
Mutagenesisi45 – 52MVKELQEI → AAKEAQEA: No nuclear export. 1 Publication8
Mutagenesisi47K → R: Little change in Tax-stimulated transactivation. No change in Tax-stimulated transactivation; when associated with R-38. 1 Publication1
Mutagenesisi115 – 120LHLAVI → AHAAVA: Greatly reduced nuclear localization. Great reduction in its ability to inhibit DNA binding of RELA. 1 Publication6
Mutagenesisi210N → A: Almost abolished ability to inhibit NF-kappa-B DNA-binding activity; when associated with A-244. 1 Publication1
Mutagenesisi234S → A: No inducible ubiquitination nor protein degradation. 1 Publication1
Mutagenesisi244N → A: Almost abolished ability to inhibit NF-kappa-B DNA-binding activity; when associated with A-210. 1 Publication1
Mutagenesisi262S → A: No inducible ubiquitination nor protein degradation. 1 Publication1
Mutagenesisi263T → A: No inducible ubiquitination nor protein degradation. 1 Publication1

Keywords - Diseasei

Disease mutation, Ectodermal dysplasia

Organism-specific databases

DisGeNETi4792
MalaCardsiNFKBIA
MIMi612132 phenotype
OpenTargetsiENSG00000100906
Orphaneti251579 Giant cell glioblastoma
251576 Gliosarcoma
98813 Hypohidrotic ectodermal dysplasia with immunodeficiency
PharmGKBiPA31601

Chemistry databases

ChEMBLiCHEMBL2898
DrugBankiDB00945 Acetylsalicylic acid

Polymorphism and mutation databases

BioMutaiNFKBIA
DMDMi126682

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000669991 – 317NF-kappa-B inhibitor alphaAdd BLAST317

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki21Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO or ubiquitin); alternate2 Publications
Cross-linki21Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Cross-linki22Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)2 Publications
Modified residuei32Phosphoserine; by IKKA and IKKE2 Publications1
Modified residuei36Phosphoserine; by IKKA, IKKB, IKKE and TBK12 Publications1
Modified residuei42Phosphotyrosine; by Tyr-kinases1 Publication1
Modified residuei210(3S)-3-hydroxyasparagine; by HIF1AN; partial1 Publication1
Modified residuei244(3S)-3-hydroxyasparagine; by HIF1AN; partial1 Publication1
Modified residuei283Phosphoserine; by CK22 Publications1
Modified residuei288Phosphoserine; by CK21 Publication1
Modified residuei291Phosphothreonine; by CK22 Publications1
Modified residuei293Phosphoserine; by CK21 Publication1
Modified residuei299Phosphothreonine; by CK21 Publication1

Post-translational modificationi

Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.3 Publications
Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.3 Publications
Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.5 Publications
Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.

Keywords - PTMi

Hydroxylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP25963
MaxQBiP25963
PaxDbiP25963
PeptideAtlasiP25963
PRIDEiP25963

PTM databases

iPTMnetiP25963
PhosphoSitePlusiP25963

Miscellaneous databases

PMAP-CutDBiP25963

Expressioni

Inductioni

Induced in adherent monocytes.

Gene expression databases

BgeeiENSG00000100906
CleanExiHS_NFKBIA
ExpressionAtlasiP25963 baseline and differential
GenevisibleiP25963 HS

Organism-specific databases

HPAiCAB003815
HPA029207

Interactioni

Subunit structurei

Interacts with RELA; the interaction requires the nuclear import signal. Interacts with NKIRAS1 and NKIRAS2. Part of a 70-90 kDa complex at least consisting of CHUK, IKBKB, NFKBIA, RELA, ELP1 and MAP3K14. Interacts with HBV protein X. Interacts with isoform 1 and isoform 2 of RWDD3; the interaction enhances sumoylation. Interacts (when phosphorylated at the 2 serine residues in the destruction motif D-S-G-X(2,3,4)-S) with BTRC. Associates with the SCF(BTRC) complex, composed of SKP1, CUL1 and BTRC; the association is mediated via interaction with BTRC. Part of a SCF(BTRC)-like complex lacking CUL1, which is associated with RELA; RELA interacts directly with NFKBIA. Interacts with PRMT2. Interacts with PRKACA in platelets; this interaction is disrupted by thrombin and collagen. Interacts with HIF1AN. Interacts with MEFV. Interacts with DDRGK1; positively regulates NFKBIA phosphorylation and degradation.10 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • enzyme binding Source: UniProtKB
  • heat shock protein binding Source: Ensembl
  • identical protein binding Source: IntAct
  • NF-kappaB binding Source: MGI
  • transcription factor binding Source: ProtInc
  • ubiquitin protein ligase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi110859, 147 interactors
CORUMiP25963
DIPiDIP-139N
ELMiP25963
IntActiP25963, 94 interactors
MINTiP25963
STRINGi9606.ENSP00000216797

Chemistry databases

BindingDBiP25963

Structurei

Secondary structure

1317
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni72 – 74Combined sources3
Helixi79 – 83Combined sources5
Beta strandi87 – 91Combined sources5
Helixi101 – 104Combined sources4
Helixi114 – 120Combined sources7
Helixi124 – 128Combined sources5
Helixi147 – 154Combined sources8
Helixi157 – 165Combined sources9
Turni167 – 170Combined sources4
Beta strandi171 – 173Combined sources3
Helixi175 – 177Combined sources3
Helixi186 – 192Combined sources7
Helixi196 – 205Combined sources10
Turni214 – 216Combined sources3
Helixi220 – 226Combined sources7
Helixi230 – 237Combined sources8
Turni238 – 240Combined sources3
Helixi253 – 256Combined sources4
Helixi263 – 270Combined sources8
Helixi275 – 277Combined sources3
Turni285 – 287Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IKNX-ray2.30D67-302[»]
1NFIX-ray2.70E/F70-282[»]
DisProtiDP00468
ProteinModelPortaliP25963
SMRiP25963
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25963

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati73 – 103ANK 1Add BLAST31
Repeati110 – 139ANK 2Add BLAST30
Repeati143 – 172ANK 3Add BLAST30
Repeati182 – 211ANK 4Add BLAST30
Repeati216 – 245ANK 5Add BLAST30

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi30 – 36Destruction motif7
Motifi45 – 54Nuclear export signal10
Motifi110 – 120Nuclear import signalAdd BLAST11

Sequence similaritiesi

Belongs to the NF-kappa-B inhibitor family.Curated

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0504 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00550000074527
HOGENOMiHOG000059576
HOVERGENiHBG018875
InParanoidiP25963
KOiK04734
OMAiGHNCLHL
OrthoDBiEOG091G0CSV
PhylomeDBiP25963
TreeFamiTF320166

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
PfamiView protein in Pfam
PF00023 Ank, 1 hit
PF12796 Ank_2, 1 hit
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 5 hits
SUPFAMiSSF48403 SSF48403, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 3 hits

Sequencei

Sequence statusi: Complete.

P25963-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFQAAERPQE WAMEGPRDGL KKERLLDDRH DSGLDSMKDE EYEQMVKELQ
60 70 80 90 100
EIRLEPQEVP RGSEPWKQQL TEDGDSFLHL AIIHEEKALT MEVIRQVKGD
110 120 130 140 150
LAFLNFQNNL QQTPLHLAVI TNQPEIAEAL LGAGCDPELR DFRGNTPLHL
160 170 180 190 200
ACEQGCLASV GVLTQSCTTP HLHSILKATN YNGHTCLHLA SIHGYLGIVE
210 220 230 240 250
LLVSLGADVN AQEPCNGRTA LHLAVDLQNP DLVSLLLKCG ADVNRVTYQG
260 270 280 290 300
YSPYQLTWGR PSTRIQQQLG QLTLENLQML PESEDEESYD TESEFTEFTE
310
DELPYDDCVF GGQRLTL
Length:317
Mass (Da):35,609
Last modified:May 1, 1992 - v1
Checksum:i088B313226786395
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03487132S → I in ADEDAID. 1 PublicationCorresponds to variant dbSNP:rs28933100Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M69043 mRNA Translation: AAA16489.1
AJ249294
, AJ249295, AJ249283, AJ249284, AJ249285, AJ249286 Genomic DNA Translation: CAB65556.2
AY033600 mRNA Translation: AAK51149.1
BT007091 mRNA Translation: AAP35754.1
AY496422 Genomic DNA Translation: AAR29599.1
AK313421 mRNA Translation: BAG36213.1
AL133163 Genomic DNA No translation available.
CH471078 Genomic DNA Translation: EAW65875.1
BC002601 mRNA Translation: AAH02601.1
BC004983 mRNA Translation: AAH04983.1
CCDSiCCDS9656.1
PIRiA39935
RefSeqiNP_065390.1, NM_020529.2
UniGeneiHs.81328

Genome annotation databases

EnsembliENST00000216797; ENSP00000216797; ENSG00000100906
GeneIDi4792
KEGGihsa:4792
UCSCiuc001wtf.5 human

Similar proteinsi

Entry informationi

Entry nameiIKBA_HUMAN
AccessioniPrimary (citable) accession number: P25963
Secondary accession number(s): B2R8L6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 25, 2018
This is version 203 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health