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Protein

Beta-3 adrenergic receptor

Gene

Adrb3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei114 – 1141Agonist or antagonistBy similarity
Binding sitei119 – 1191Agonist or antagonistBy similarity

GO - Molecular functioni

GO - Biological processi

  • activation of adenylate cyclase activity Source: HGNC
  • adenylate cyclase-activating adrenergic receptor signaling pathway Source: GO_Central
  • adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: MGI
  • aging Source: Ensembl
  • brown fat cell differentiation Source: MGI
  • cell-cell signaling Source: GO_Central
  • diet induced thermogenesis Source: MGI
  • eating behavior Source: Ensembl
  • heat generation Source: MGI
  • negative regulation of multicellular organism growth Source: MGI
  • positive regulation of MAPK cascade Source: HGNC
  • response to antibiotic Source: Ensembl
  • response to cold Source: MGI
  • vasodilation by norepinephrine-epinephrine involved in regulation of systemic arterial blood pressure Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-390696. Adrenoceptors.
R-MMU-418555. G alpha (s) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-3 adrenergic receptor
Alternative name(s):
Beta-3 adrenoreceptor
Short name:
Beta-3 adrenoceptor
Gene namesi
Name:Adrb3
Synonyms:Adrb3r, B3bar
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:87939. Adrb3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3636ExtracellularBy similarityAdd
BLAST
Transmembranei37 – 6024Helical; Name=1By similarityAdd
BLAST
Topological domaini61 – 699CytoplasmicBy similarity
Transmembranei70 – 8819Helical; Name=2By similarityAdd
BLAST
Topological domaini89 – 10820ExtracellularBy similarityAdd
BLAST
Transmembranei109 – 13022Helical; Name=3By similarityAdd
BLAST
Topological domaini131 – 15222CytoplasmicBy similarityAdd
BLAST
Transmembranei153 – 17523Helical; Name=4By similarityAdd
BLAST
Topological domaini176 – 20025ExtracellularBy similarityAdd
BLAST
Transmembranei201 – 22222Helical; Name=5By similarityAdd
BLAST
Topological domaini223 – 28967CytoplasmicBy similarityAdd
BLAST
Transmembranei290 – 31122Helical; Name=6By similarityAdd
BLAST
Topological domaini312 – 32312ExtracellularBy similarityAdd
BLAST
Transmembranei324 – 34421Helical; Name=7By similarityAdd
BLAST
Topological domaini345 – 40056CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • membrane Source: MGI
  • nucleus Source: Ensembl
  • receptor complex Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2111395.
GuidetoPHARMACOLOGYi30.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400Beta-3 adrenergic receptorPRO_0000069146Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi8 – 81N-linked (GlcNAc...)Sequence analysis
Glycosylationi26 – 261N-linked (GlcNAc...)Sequence analysis
Disulfide bondi107 ↔ 193PROSITE-ProRule annotation
Disulfide bondi186 ↔ 192PROSITE-ProRule annotation
Lipidationi358 – 3581S-palmitoyl cysteineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP25962.
PeptideAtlasiP25962.
PRIDEiP25962.

PTM databases

iPTMnetiP25962.

Expressioni

Tissue specificityi

White and brown adipose tissues, and digestive tract. Isoform B highest in brain.

Gene expression databases

BgeeiP25962.
CleanExiMM_ADRB3.
ExpressionAtlasiP25962. baseline and differential.
GenevisibleiP25962. MM.

Interactioni

Subunit structurei

Interacts with ARRDC3.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198008. 1 interaction.
IntActiP25962. 1 interaction.
MINTiMINT-146553.
STRINGi10090.ENSMUSP00000080162.

Chemistry

BindingDBiP25962.

Structurei

3D structure databases

ProteinModelPortaliP25962.
SMRiP25962. Positions 34-385.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni195 – 20915Agonist and antagonist bindingBy similarityAdd
BLAST
Regioni302 – 3098Agonist and antagonist bindingBy similarity
Regioni329 – 3335Agonist and antagonist bindingBy similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB3 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118774.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP25962.
KOiK04143.
OMAiAPIMSKW.
OrthoDBiEOG7BS4BS.
PhylomeDBiP25962.
TreeFamiTF316350.

Family and domain databases

InterProiIPR002233. ADR_fam.
IPR000681. ADRB3_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01103. ADRENERGICR.
PR00563. ADRENRGCB3AR.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P25962-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPWPHRNGS LALWSDAPTL DPSAANTSGL PGVPWAAALA GALLALATVG
60 70 80 90 100
GNLLVIIAIA RTPRLQTITN VFVTSLAAAD LVVGLLVMPP GATLALTGHW
110 120 130 140 150
PLGETGCELW TSVDVLCVTA SIETLCALAV DRYLAVTNPL RYGTLVTKRR
160 170 180 190 200
ARAAVVLVWI VSAAVSFAPI MSQWWRVGAD AEAQECHSNP RCCSFASNMP
210 220 230 240 250
YALLSSSVSF YLPLLVMLFV YARVFVVAKR QRHLLRRELG RFSPEESPPS
260 270 280 290 300
PSRSPSPATG GTPAAPDGVP PCGRRPARLL PLREHRALRT LGLIMGIFSL
310 320 330 340 350
CWLPFFLANV LRALAGPSLV PSGVFIALNW LGYANSAFNP VIYCRSPDFR
360 370 380 390 400
DAFRRLLCSY GGRGPEEPRA VTFPASPVEA RQSPPLNRFD GYEGARPFPT
Length:400
Mass (Da):43,006
Last modified:February 1, 1994 - v2
Checksum:i374E072C01DFA32E
GO
Isoform B (identifier: P25962-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     388-400: RFDGYEGARPFPT → SSLLREPRHLYTCLGYP

Show »
Length:404
Mass (Da):43,499
Checksum:i06E3D91241B63894
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei388 – 40013RFDGY…RPFPT → SSLLREPRHLYTCLGYP in isoform B. 2 PublicationsVSP_001864Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72862 Genomic DNA. Translation: CAA51384.1.
X60438 Genomic DNA. Translation: CAA42966.1.
AF193027 mRNA. Translation: AAF05768.1.
BC132000 mRNA. Translation: AAI32001.1.
S53290 mRNA. Translation: AAB24836.1.
CCDSiCCDS22213.1. [P25962-1]
PIRiI77910.
S32804.
RefSeqiNP_038490.2. NM_013462.3. [P25962-1]
XP_006509050.1. XM_006508987.2. [P25962-1]
XP_006509051.1. XM_006508988.2. [P25962-1]
XP_011240418.1. XM_011242116.1. [P25962-2]
XP_011240419.1. XM_011242117.1. [P25962-2]
UniGeneiMm.278475.

Genome annotation databases

EnsembliENSMUST00000081438; ENSMUSP00000080162; ENSMUSG00000031489. [P25962-1]
ENSMUST00000117565; ENSMUSP00000113732; ENSMUSG00000031489. [P25962-2]
ENSMUST00000121838; ENSMUSP00000113006; ENSMUSG00000031489. [P25962-1]
GeneIDi11556.
KEGGimmu:11556.
UCSCiuc009lie.1. mouse. [P25962-1]
uc009lig.1. mouse. [P25962-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72862 Genomic DNA. Translation: CAA51384.1.
X60438 Genomic DNA. Translation: CAA42966.1.
AF193027 mRNA. Translation: AAF05768.1.
BC132000 mRNA. Translation: AAI32001.1.
S53290 mRNA. Translation: AAB24836.1.
CCDSiCCDS22213.1. [P25962-1]
PIRiI77910.
S32804.
RefSeqiNP_038490.2. NM_013462.3. [P25962-1]
XP_006509050.1. XM_006508987.2. [P25962-1]
XP_006509051.1. XM_006508988.2. [P25962-1]
XP_011240418.1. XM_011242116.1. [P25962-2]
XP_011240419.1. XM_011242117.1. [P25962-2]
UniGeneiMm.278475.

3D structure databases

ProteinModelPortaliP25962.
SMRiP25962. Positions 34-385.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198008. 1 interaction.
IntActiP25962. 1 interaction.
MINTiMINT-146553.
STRINGi10090.ENSMUSP00000080162.

Chemistry

BindingDBiP25962.
ChEMBLiCHEMBL2111395.
GuidetoPHARMACOLOGYi30.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP25962.

Proteomic databases

PaxDbiP25962.
PeptideAtlasiP25962.
PRIDEiP25962.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000081438; ENSMUSP00000080162; ENSMUSG00000031489. [P25962-1]
ENSMUST00000117565; ENSMUSP00000113732; ENSMUSG00000031489. [P25962-2]
ENSMUST00000121838; ENSMUSP00000113006; ENSMUSG00000031489. [P25962-1]
GeneIDi11556.
KEGGimmu:11556.
UCSCiuc009lie.1. mouse. [P25962-1]
uc009lig.1. mouse. [P25962-2]

Organism-specific databases

CTDi155.
MGIiMGI:87939. Adrb3.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118774.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP25962.
KOiK04143.
OMAiAPIMSKW.
OrthoDBiEOG7BS4BS.
PhylomeDBiP25962.
TreeFamiTF316350.

Enzyme and pathway databases

ReactomeiR-MMU-390696. Adrenoceptors.
R-MMU-418555. G alpha (s) signalling events.

Miscellaneous databases

PROiP25962.
SOURCEiSearch...

Gene expression databases

BgeeiP25962.
CleanExiMM_ADRB3.
ExpressionAtlasiP25962. baseline and differential.
GenevisibleiP25962. MM.

Family and domain databases

InterProiIPR002233. ADR_fam.
IPR000681. ADRB3_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01103. ADRENERGICR.
PR00563. ADRENRGCB3AR.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of the mouse beta 3-adrenergic receptor: relationship with the atypical receptor of adipocytes."
    Nahmias C., Blin N., Elalouf J.-M., Mattei M.-G., Strosberg A.D., Emorine L.J.
    EMBO J. 10:3721-3727(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SWR/J.
  2. "The promoter and intron/exon structure of the human and mouse beta 3-adrenergic-receptor genes."
    van Spronsen A., Nahmias C., Krief S., Briend-Sutren M.-M., Strosberg A.D., Emorine L.J.
    Eur. J. Biochem. 213:1117-1124(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION.
  3. "Alternative splicing generates two isoforms of the beta3-adrenoceptor which are differentially expressed in mouse tissues."
    Evans B.A., Papaioannou M., Hamilton S., Summers R.J.
    Br. J. Pharmacol. 127:1525-1531(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
    Strain: C57BL/6J.
    Tissue: Brown adipose tissue.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
    Tissue: Brain.
  5. "Rodent and human beta 3-adrenergic receptor genes contain an intron within the protein-coding block."
    Granneman J.G., Lahners K.N., Rao D.D.
    Mol. Pharmacol. 42:964-970(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 378-400.
    Tissue: Adipose tissue.

Entry informationi

Entry nameiADRB3_MOUSE
AccessioniPrimary (citable) accession number: P25962
Secondary accession number(s): A2RS74, Q9QZ98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: February 1, 1994
Last modified: July 6, 2016
This is version 158 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.