P25961 (PTH1R_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Parathyroid hormone/parathyroid hormone-related peptide receptor

Alternative name(s):
PTH/PTHrP type I receptor
Short name=PTH/PTHr receptor
Parathyroid hormone 1 receptor
Short name=PTH1 receptor

Gene names

Name:	Pth1r
Synonyms:	Pthr, Pthr1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This is a receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.
Subunit structure	Interacts (via N-terminal extracellular domain) with PTHLH and PTH. Homodimer in the absence of bound ligand. Peptide hormone binding leads to dissociation of the homodimer. Interacts (via C-terminus) with the heterodimer formed by GNG2 and GNB1 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 2 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adenylate cyclase-activating G-protein coupled receptor signaling pathway Inferred from direct assay PubMed 19608645. Source: BHF-UCL aging Inferred from expression pattern PubMed 12647298. Source: RGD bone mineralization Inferred from electronic annotation. Source: Compara bone resorption Inferred from electronic annotation. Source: Compara cell maturation Inferred from electronic annotation. Source: Compara chondrocyte differentiation Inferred from electronic annotation. Source: Compara elevation of cytosolic calcium ion concentration Inferred from direct assay Ref.1. Source: RGD negative regulation of cell proliferation Inferred from electronic annotation. Source: Compara osteoblast development Inferred from electronic annotation. Source: Compara phospholipase C-activating G-protein coupled receptor signaling pathway Inferred by curator PubMed 19608645. Source: BHF-UCL positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of inositol phosphate biosynthetic process Inferred from direct assay PubMed 19608645. Source: BHF-UCL
Cellular_component	apical plasma membrane Inferred from direct assay PubMed 19608645. Source: BHF-UCL basolateral plasma membrane Inferred from direct assay PubMed 19608645. Source: BHF-UCL brush border membrane Inferred from direct assay PubMed 12647298. Source: RGD cytoplasm Inferred from direct assay PubMed 12647298. Source: RGD integral to plasma membrane Inferred from electronic annotation. Source: Compara nucleus Inferred from direct assay PubMed 12647298. Source: RGD receptor complex Inferred from direct assay PubMed 19608645. Source: BHF-UCL
Molecular_function	parathyroid hormone receptor activity Inferred from direct assay PubMed 19608645. Source: BHF-UCL peptide hormone binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 591	565	Parathyroid hormone/parathyroid hormone-related peptide receptor		PRO_0000012848
Regions
	Topological domain	27 – 188	162	Extracellular Potential
	Transmembrane	189 – 212	24	Helical; Name=1; Potential
	Topological domain	213 – 219	7	Cytoplasmic Potential
	Transmembrane	220 – 239	20	Helical; Name=2; Potential
	Topological domain	240 – 282	43	Extracellular Potential
	Transmembrane	283 – 306	24	Helical; Name=3; Potential
	Topological domain	307 – 320	14	Cytoplasmic Potential
	Transmembrane	321 – 342	22	Helical; Name=4; Potential
	Topological domain	343 – 361	19	Extracellular Potential
	Transmembrane	362 – 382	21	Helical; Name=5; Potential
	Topological domain	383 – 409	27	Cytoplasmic Potential
	Transmembrane	410 – 428	19	Helical; Name=6; Potential
	Topological domain	429 – 440	12	Extracellular Potential
	Transmembrane	441 – 463	23	Helical; Name=7; Potential
	Topological domain	464 – 591	128	Cytoplasmic Potential
	Motif	474 – 477	4	Important for interaction with G proteins By similarity
Amino acid modifications
	Glycosylation	151	1	N-linked (GlcNAc...) Potential
	Glycosylation	161	1	N-linked (GlcNAc...) Potential
	Glycosylation	166	1	N-linked (GlcNAc...) Potential
	Glycosylation	176	1	N-linked (GlcNAc...) Potential
	Disulfide bond	48 ↔ 117		By similarity
	Disulfide bond	108 ↔ 148		By similarity
	Disulfide bond	131 ↔ 170		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P25961 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 21944F3051B9E9C1	FASTA	591	66,261
	10 20 30 40 50 60 MGAARIAPSL ALLLCCPVLS SAYALVDADD VFTKEEQIFL LHRAQAQCDK LLKEVLHTAA 70 80 90 100 110 120 NIMESDKGWT PASTSGKPRK EKASGKFYPE SKENKDVPTG SRRRGRPCLP EWDNIVCWPL 130 140 150 160 170 180 GAPGEVVAVP CPDYIYDFNH KGHAYRRCDR NGSWEVVPGH NRTWANYSEC LKFMTNETRE 190 200 210 220 230 240 REVFDRLGMI YTVGYSMSLA SLTVAVLILA YFRRLHCTRN YIHMHMFLSF MLRAASIFVK 250 260 270 280 290 300 DAVLYSGFTL DEAERLTEEE LHIIAQVPPP PAAAAVGYAG CRVAVTFFLY FLATNYYWIL 310 320 330 340 350 360 VEGLYLHSLI FMAFFSEKKY LWGFTIFGWG LPAVFVAVWV GVRATLANTG CWDLSSGHKK 370 380 390 400 410 420 WIIQVPILAS VVLNFILFIN IIRVLATKLR ETNAGRCDTR QQYRKLLRST LVLVPLFGVH 430 440 450 460 470 480 YTVFMALPYT EVSGTLWQIQ MHYEMLFNSF QGFFVAIIYC FCNGEVQAEI RKSWSRWTLA 490 500 510 520 530 540 LDFKRKARSG SSSYSYGPMV SHTSVTNVGP RAGLSLPLSP RLPPATTNGH SQLPGHAKPG 550 560 570 580 590 APATETETLP VTMAVPKDDG FLNGSCSGLD EEASGSARPP PLLQEEWETV M

References

[1]	"Expression cloning of a common receptor for parathyroid hormone and parathyroid hormone-related peptide from rat osteoblast-like cells: a single receptor stimulates intracellular accumulation of both cAMP and inositol trisphosphates and increases intracellular free calcium." Abou-Samra A.-B., Jueppner H., Force T., Freeman M.W., Kong X.-F., Schipani E., Urena P., Richards J., Bonventre J.V., Potts J.T. Jr., Kronenberg H.M., Segre G.V. Proc. Natl. Acad. Sci. U.S.A. 89:2732-2736(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Bone.
[2]	"Cloning of a parathyroid hormone/parathyroid hormone-related peptide receptor (PTHR) cDNA from a rat osteosarcoma (UMR 106) cell line: chromosomal assignment of the gene in the human, mouse, and rat genomes." Pausova Z., Bourdon J., Clayton D., Mattei M.-G., Seldin M.F., Janicic N., Riviere M., Szpirer J., Levan G., Szpirer C. Genomics 20:20-26(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M77184 mRNA. Translation: AAA41811.1. L19475 mRNA. Translation: AAA68098.1.
IPI	IPI00194506.
PIR	I54195.
RefSeq	NP_064458.1. NM_020073.2.
UniGene	Rn.48025.
3D structure databases
ProteinModelPortal	P25961.
SMR	P25961. Positions 24-175.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-46025N.
STRING	10116.ENSRNOP00000028435.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P25961.
Proteomic databases
PaxDb	P25961.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000028435; ENSRNOP00000028435; ENSRNOG00000020948.
GeneID	56813.
KEGG	rno:56813.
UCSC	RGD:3442. rat.
Organism-specific databases
CTD	5745.
RGD	3442. Pth1r.
Phylogenomic databases
eggNOG	NOG293423.
GeneTree	ENSGT00650000093116.
HOGENOM	HOG000008248.
HOVERGEN	HBG008318.
InParanoid	P25961.
KO	K04585.
OMA	WIIQVPI.
OrthoDB	EOG4XWFXR.
Gene expression databases
ArrayExpress	P25961.
Genevestigator	P25961.
GermOnline	ENSRNOG00000020948. Rattus norvegicus.
Family and domain databases
InterPro	IPR017981. GPCR_2-like. IPR001879. GPCR_2_extracellular_dom. IPR002170. GPCR_2_parathyroid_rcpt. IPR000832. GPCR_2_secretin-like. IPR017983. GPCR_2_secretin-like_CS. [Graphical view]
PANTHER	PTHR12011:SF24. PTHR12011:SF24. 1 hit.
Pfam	PF00002. 7tm_2. 1 hit. PF02793. HRM. 1 hit. [Graphical view]
PRINTS	PR00249. GPCRSECRETIN. PR00393. PTRHORMONER.
SMART	SM00008. HormR. 1 hit. [Graphical view]
PROSITE	PS00649. G_PROTEIN_RECEP_F2_1. 1 hit. PS00650. G_PROTEIN_RECEP_F2_2. 1 hit. PS50227. G_PROTEIN_RECEP_F2_3. 1 hit. PS50261. G_PROTEIN_RECEP_F2_4. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P25961.
ChEMBL	CHEMBL6038.
NextBio	611215.

Entry information

Entry name

PTH1R_RAT

Accession

Primary (citable) accession number: P25961

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	April 3, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program