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Protein

Parathyroid hormone/parathyroid hormone-related peptide receptor

Gene

Pth1r

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

This is a receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.

GO - Molecular functioni

  • parathyroid hormone receptor activity Source: BHF-UCL
  • peptide hormone binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiREACT_287445. G alpha (s) signalling events.
REACT_323768. Class B/2 (Secretin family receptors).

Names & Taxonomyi

Protein namesi
Recommended name:
Parathyroid hormone/parathyroid hormone-related peptide receptor
Alternative name(s):
PTH/PTHrP type I receptor
Short name:
PTH/PTHr receptor
Parathyroid hormone 1 receptor
Short name:
PTH1 receptor
Gene namesi
Name:Pth1r
Synonyms:Pthr, Pthr1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi3442. Pth1r.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini27 – 188162ExtracellularSequence AnalysisAdd
BLAST
Transmembranei189 – 21224Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini213 – 2197CytoplasmicSequence Analysis
Transmembranei220 – 23920Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini240 – 28243ExtracellularSequence AnalysisAdd
BLAST
Transmembranei283 – 30624Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini307 – 32014CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei321 – 34222Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini343 – 36119ExtracellularSequence AnalysisAdd
BLAST
Transmembranei362 – 38221Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini383 – 40927CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei410 – 42819Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini429 – 44012ExtracellularSequence AnalysisAdd
BLAST
Transmembranei441 – 46323Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini464 – 591128CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • apical plasma membrane Source: BHF-UCL
  • basolateral plasma membrane Source: BHF-UCL
  • brush border membrane Source: RGD
  • cytoplasm Source: RGD
  • cytosol Source: GOC
  • extracellular exosome Source: Ensembl
  • integral component of plasma membrane Source: Ensembl
  • nucleus Source: RGD
  • plasma membrane Source: RGD
  • receptor complex Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 591565Parathyroid hormone/parathyroid hormone-related peptide receptorPRO_0000012848Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi48 ↔ 117By similarity
Disulfide bondi108 ↔ 148By similarity
Disulfide bondi131 ↔ 170By similarity
Glycosylationi151 – 1511N-linked (GlcNAc...)Sequence Analysis
Glycosylationi161 – 1611N-linked (GlcNAc...)Sequence Analysis
Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence Analysis
Glycosylationi176 – 1761N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP25961.

PTM databases

PhosphoSiteiP25961.

Expressioni

Gene expression databases

ExpressionAtlasiP25961. baseline.
GenevisibleiP25961. RN.

Interactioni

Subunit structurei

Interacts (via N-terminal extracellular domain) with PTHLH and PTH. Homodimer in the absence of bound ligand. Peptide hormone binding leads to dissociation of the homodimer. Interacts (via C-terminus) with the heterodimer formed by GNG2 and GNB1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi248587. 2 interactions.
DIPiDIP-46025N.
STRINGi10116.ENSRNOP00000028435.

Structurei

3D structure databases

ProteinModelPortaliP25961.
SMRiP25961. Positions 24-175.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi474 – 4774Important for interaction with G proteinsBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG293423.
GeneTreeiENSGT00760000118800.
HOGENOMiHOG000008248.
HOVERGENiHBG008318.
InParanoidiP25961.
KOiK04585.
OMAiYAGCRVA.
OrthoDBiEOG7TF78W.
PhylomeDBiP25961.
TreeFamiTF315710.

Family and domain databases

InterProiIPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR002170. GPCR_2_parathyroid_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view]
PANTHERiPTHR12011:SF24. PTHR12011:SF24. 1 hit.
PfamiPF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view]
PRINTSiPR00249. GPCRSECRETIN.
PR00393. PTRHORMONER.
SMARTiSM00008. HormR. 1 hit.
[Graphical view]
PROSITEiPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25961-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAARIAPSL ALLLCCPVLS SAYALVDADD VFTKEEQIFL LHRAQAQCDK
60 70 80 90 100
LLKEVLHTAA NIMESDKGWT PASTSGKPRK EKASGKFYPE SKENKDVPTG
110 120 130 140 150
SRRRGRPCLP EWDNIVCWPL GAPGEVVAVP CPDYIYDFNH KGHAYRRCDR
160 170 180 190 200
NGSWEVVPGH NRTWANYSEC LKFMTNETRE REVFDRLGMI YTVGYSMSLA
210 220 230 240 250
SLTVAVLILA YFRRLHCTRN YIHMHMFLSF MLRAASIFVK DAVLYSGFTL
260 270 280 290 300
DEAERLTEEE LHIIAQVPPP PAAAAVGYAG CRVAVTFFLY FLATNYYWIL
310 320 330 340 350
VEGLYLHSLI FMAFFSEKKY LWGFTIFGWG LPAVFVAVWV GVRATLANTG
360 370 380 390 400
CWDLSSGHKK WIIQVPILAS VVLNFILFIN IIRVLATKLR ETNAGRCDTR
410 420 430 440 450
QQYRKLLRST LVLVPLFGVH YTVFMALPYT EVSGTLWQIQ MHYEMLFNSF
460 470 480 490 500
QGFFVAIIYC FCNGEVQAEI RKSWSRWTLA LDFKRKARSG SSSYSYGPMV
510 520 530 540 550
SHTSVTNVGP RAGLSLPLSP RLPPATTNGH SQLPGHAKPG APATETETLP
560 570 580 590
VTMAVPKDDG FLNGSCSGLD EEASGSARPP PLLQEEWETV M
Length:591
Mass (Da):66,261
Last modified:May 1, 1992 - v1
Checksum:i21944F3051B9E9C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77184 mRNA. Translation: AAA41811.1.
L19475 mRNA. Translation: AAA68098.1.
PIRiI54195.
RefSeqiNP_064458.1. NM_020073.2.
XP_006244060.1. XM_006243998.2.
XP_006244061.1. XM_006243999.2.
UniGeneiRn.48025.

Genome annotation databases

EnsembliENSRNOT00000028435; ENSRNOP00000028435; ENSRNOG00000020948.
GeneIDi56813.
KEGGirno:56813.
UCSCiRGD:3442. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77184 mRNA. Translation: AAA41811.1.
L19475 mRNA. Translation: AAA68098.1.
PIRiI54195.
RefSeqiNP_064458.1. NM_020073.2.
XP_006244060.1. XM_006243998.2.
XP_006244061.1. XM_006243999.2.
UniGeneiRn.48025.

3D structure databases

ProteinModelPortaliP25961.
SMRiP25961. Positions 24-175.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248587. 2 interactions.
DIPiDIP-46025N.
STRINGi10116.ENSRNOP00000028435.

Chemistry

BindingDBiP25961.
ChEMBLiCHEMBL6038.
GuidetoPHARMACOLOGYi331.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP25961.

Proteomic databases

PaxDbiP25961.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000028435; ENSRNOP00000028435; ENSRNOG00000020948.
GeneIDi56813.
KEGGirno:56813.
UCSCiRGD:3442. rat.

Organism-specific databases

CTDi5745.
RGDi3442. Pth1r.

Phylogenomic databases

eggNOGiNOG293423.
GeneTreeiENSGT00760000118800.
HOGENOMiHOG000008248.
HOVERGENiHBG008318.
InParanoidiP25961.
KOiK04585.
OMAiYAGCRVA.
OrthoDBiEOG7TF78W.
PhylomeDBiP25961.
TreeFamiTF315710.

Enzyme and pathway databases

ReactomeiREACT_287445. G alpha (s) signalling events.
REACT_323768. Class B/2 (Secretin family receptors).

Miscellaneous databases

NextBioi611215.
PROiP25961.

Gene expression databases

ExpressionAtlasiP25961. baseline.
GenevisibleiP25961. RN.

Family and domain databases

InterProiIPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR002170. GPCR_2_parathyroid_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view]
PANTHERiPTHR12011:SF24. PTHR12011:SF24. 1 hit.
PfamiPF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view]
PRINTSiPR00249. GPCRSECRETIN.
PR00393. PTRHORMONER.
SMARTiSM00008. HormR. 1 hit.
[Graphical view]
PROSITEiPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Expression cloning of a common receptor for parathyroid hormone and parathyroid hormone-related peptide from rat osteoblast-like cells: a single receptor stimulates intracellular accumulation of both cAMP and inositol trisphosphates and increases intracellular free calcium."
    Abou-Samra A.-B., Jueppner H., Force T., Freeman M.W., Kong X.-F., Schipani E., Urena P., Richards J., Bonventre J.V., Potts J.T. Jr., Kronenberg H.M., Segre G.V.
    Proc. Natl. Acad. Sci. U.S.A. 89:2732-2736(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Bone.
  2. "Cloning of a parathyroid hormone/parathyroid hormone-related peptide receptor (PTHR) cDNA from a rat osteosarcoma (UMR 106) cell line: chromosomal assignment of the gene in the human, mouse, and rat genomes."
    Pausova Z., Bourdon J., Clayton D., Mattei M.-G., Seldin M.F., Janicic N., Riviere M., Szpirer J., Levan G., Szpirer C.
    Genomics 20:20-26(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiPTH1R_RAT
AccessioniPrimary (citable) accession number: P25961
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 24, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.