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P25961 (PTH1R_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Parathyroid hormone/parathyroid hormone-related peptide receptor
Alternative name(s):
PTH/PTHrP type I receptor
Short name=PTH/PTHr receptor
Parathyroid hormone 1 receptor
Short name=PTH1 receptor
Gene names
Name:Pth1r
Synonyms:Pthr, Pthr1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length591 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This is a receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.

Subunit structure

Interacts (via N-terminal extracellular domain) with PTHLH and PTH. Homodimer in the absence of bound ligand. Peptide hormone binding leads to dissociation of the homodimer. Interacts (via C-terminus) with the heterodimer formed by GNG2 and GNB1 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the G-protein coupled receptor 2 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger

Inferred from mutant phenotype PubMed 8662729. Source: RGD

adenylate cyclase-activating G-protein coupled receptor signaling pathway

Inferred from direct assay PubMed 19608645. Source: BHF-UCL

adenylate cyclase-modulating G-protein coupled receptor signaling pathway

Inferred from direct assay PubMed 19608645. Source: BHF-UCL

aging

Inferred from expression pattern PubMed 12647298. Source: RGD

bone mineralization

Inferred from electronic annotation. Source: Ensembl

bone resorption

Inferred from electronic annotation. Source: Ensembl

cell maturation

Inferred from electronic annotation. Source: Ensembl

chondrocyte differentiation

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

osteoblast development

Inferred from electronic annotation. Source: Ensembl

phospholipase C-activating G-protein coupled receptor signaling pathway

Inferred by curator PubMed 19608645. Source: BHF-UCL

positive regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of cytosolic calcium ion concentration

Inferred from direct assay Ref.1. Source: RGD

positive regulation of inositol phosphate biosynthetic process

Inferred from direct assay PubMed 19608645. Source: BHF-UCL

   Cellular_componentapical plasma membrane

Inferred from direct assay PubMed 19608645. Source: BHF-UCL

basolateral plasma membrane

Inferred from direct assay PubMed 19608645. Source: BHF-UCL

brush border membrane

Inferred from direct assay PubMed 12647298. Source: RGD

cytoplasm

Inferred from direct assay PubMed 12647298. Source: RGD

integral component of plasma membrane

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from direct assay PubMed 12647298. Source: RGD

plasma membrane

Inferred from mutant phenotype PubMed 8662729. Source: RGD

receptor complex

Inferred from direct assay PubMed 19608645. Source: BHF-UCL

   Molecular_functionparathyroid hormone receptor activity

Inferred from direct assay PubMed 19608645. Source: BHF-UCL

peptide hormone binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 591565Parathyroid hormone/parathyroid hormone-related peptide receptor
PRO_0000012848

Regions

Topological domain27 – 188162Extracellular Potential
Transmembrane189 – 21224Helical; Name=1; Potential
Topological domain213 – 2197Cytoplasmic Potential
Transmembrane220 – 23920Helical; Name=2; Potential
Topological domain240 – 28243Extracellular Potential
Transmembrane283 – 30624Helical; Name=3; Potential
Topological domain307 – 32014Cytoplasmic Potential
Transmembrane321 – 34222Helical; Name=4; Potential
Topological domain343 – 36119Extracellular Potential
Transmembrane362 – 38221Helical; Name=5; Potential
Topological domain383 – 40927Cytoplasmic Potential
Transmembrane410 – 42819Helical; Name=6; Potential
Topological domain429 – 44012Extracellular Potential
Transmembrane441 – 46323Helical; Name=7; Potential
Topological domain464 – 591128Cytoplasmic Potential
Motif474 – 4774Important for interaction with G proteins By similarity

Amino acid modifications

Glycosylation1511N-linked (GlcNAc...) Potential
Glycosylation1611N-linked (GlcNAc...) Potential
Glycosylation1661N-linked (GlcNAc...) Potential
Glycosylation1761N-linked (GlcNAc...) Potential
Disulfide bond48 ↔ 117 By similarity
Disulfide bond108 ↔ 148 By similarity
Disulfide bond131 ↔ 170 By similarity

Sequences

Sequence LengthMass (Da)Tools
P25961 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: 21944F3051B9E9C1

FASTA59166,261
        10         20         30         40         50         60 
MGAARIAPSL ALLLCCPVLS SAYALVDADD VFTKEEQIFL LHRAQAQCDK LLKEVLHTAA 

        70         80         90        100        110        120 
NIMESDKGWT PASTSGKPRK EKASGKFYPE SKENKDVPTG SRRRGRPCLP EWDNIVCWPL 

       130        140        150        160        170        180 
GAPGEVVAVP CPDYIYDFNH KGHAYRRCDR NGSWEVVPGH NRTWANYSEC LKFMTNETRE 

       190        200        210        220        230        240 
REVFDRLGMI YTVGYSMSLA SLTVAVLILA YFRRLHCTRN YIHMHMFLSF MLRAASIFVK 

       250        260        270        280        290        300 
DAVLYSGFTL DEAERLTEEE LHIIAQVPPP PAAAAVGYAG CRVAVTFFLY FLATNYYWIL 

       310        320        330        340        350        360 
VEGLYLHSLI FMAFFSEKKY LWGFTIFGWG LPAVFVAVWV GVRATLANTG CWDLSSGHKK 

       370        380        390        400        410        420 
WIIQVPILAS VVLNFILFIN IIRVLATKLR ETNAGRCDTR QQYRKLLRST LVLVPLFGVH 

       430        440        450        460        470        480 
YTVFMALPYT EVSGTLWQIQ MHYEMLFNSF QGFFVAIIYC FCNGEVQAEI RKSWSRWTLA 

       490        500        510        520        530        540 
LDFKRKARSG SSSYSYGPMV SHTSVTNVGP RAGLSLPLSP RLPPATTNGH SQLPGHAKPG 

       550        560        570        580        590 
APATETETLP VTMAVPKDDG FLNGSCSGLD EEASGSARPP PLLQEEWETV M 

« Hide

References

[1]"Expression cloning of a common receptor for parathyroid hormone and parathyroid hormone-related peptide from rat osteoblast-like cells: a single receptor stimulates intracellular accumulation of both cAMP and inositol trisphosphates and increases intracellular free calcium."
Abou-Samra A.-B., Jueppner H., Force T., Freeman M.W., Kong X.-F., Schipani E., Urena P., Richards J., Bonventre J.V., Potts J.T. Jr., Kronenberg H.M., Segre G.V.
Proc. Natl. Acad. Sci. U.S.A. 89:2732-2736(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Bone.
[2]"Cloning of a parathyroid hormone/parathyroid hormone-related peptide receptor (PTHR) cDNA from a rat osteosarcoma (UMR 106) cell line: chromosomal assignment of the gene in the human, mouse, and rat genomes."
Pausova Z., Bourdon J., Clayton D., Mattei M.-G., Seldin M.F., Janicic N., Riviere M., Szpirer J., Levan G., Szpirer C.
Genomics 20:20-26(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M77184 mRNA. Translation: AAA41811.1.
L19475 mRNA. Translation: AAA68098.1.
PIRI54195.
RefSeqNP_064458.1. NM_020073.2.
XP_006244060.1. XM_006243998.1.
XP_006244061.1. XM_006243999.1.
UniGeneRn.48025.

3D structure databases

ProteinModelPortalP25961.
SMRP25961. Positions 24-175.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid248587. 2 interactions.
DIPDIP-46025N.
STRING10116.ENSRNOP00000028435.

Chemistry

BindingDBP25961.
ChEMBLCHEMBL6038.
GuidetoPHARMACOLOGY331.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP25961.

Proteomic databases

PaxDbP25961.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000028435; ENSRNOP00000028435; ENSRNOG00000020948.
GeneID56813.
KEGGrno:56813.
UCSCRGD:3442. rat.

Organism-specific databases

CTD5745.
RGD3442. Pth1r.

Phylogenomic databases

eggNOGNOG293423.
GeneTreeENSGT00750000117453.
HOGENOMHOG000008248.
HOVERGENHBG008318.
InParanoidP25961.
KOK04585.
OMAWIIQVPI.
OrthoDBEOG7TF78W.
PhylomeDBP25961.
TreeFamTF315710.

Gene expression databases

GenevestigatorP25961.

Family and domain databases

InterProIPR017981. GPCR_2-like.
IPR001879. GPCR_2_extracellular_dom.
IPR002170. GPCR_2_parathyroid_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
[Graphical view]
PANTHERPTHR12011:SF24. PTHR12011:SF24. 1 hit.
PfamPF00002. 7tm_2. 1 hit.
PF02793. HRM. 1 hit.
[Graphical view]
PRINTSPR00249. GPCRSECRETIN.
PR00393. PTRHORMONER.
SMARTSM00008. HormR. 1 hit.
[Graphical view]
PROSITEPS00649. G_PROTEIN_RECEP_F2_1. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio611215.
PROP25961.

Entry information

Entry namePTH1R_RAT
AccessionPrimary (citable) accession number: P25961
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries