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Protein

Type 4 prepilin-like proteins leader peptide-processing enzyme

Gene

gspO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue.By similarity

Catalytic activityi

Typically cleaves a -Gly-|-Phe- bond to release an N-terminal, basic peptide of 5-8 residues from type IV prepilin, and then N-methylates the new N-terminal amino group, the methyl donor being S-adenosyl-L-methionine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Transferase

Keywords - Biological processi

Transport

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG11359-MONOMER.
ECOL316407:JW3297-MONOMER.
MetaCyc:EG11359-MONOMER.

Protein family/group databases

MEROPSiA24.A10.

Names & Taxonomyi

Protein namesi
Recommended name:
Type 4 prepilin-like proteins leader peptide-processing enzyme
Including the following 2 domains:
Leader peptidase (EC:3.4.23.43)
Alternative name(s):
Prepilin peptidase
N-methyltransferase (EC:2.1.1.-)
Gene namesi
Name:gspO
Synonyms:hofD, hopD, hopO, yheC
Ordered Locus Names:b3335, JW3297
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11359. gspO.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 22PeriplasmicSequence analysis
Transmembranei3 – 2321HelicalSequence analysisAdd
BLAST
Topological domaini24 – 6744CytoplasmicSequence analysisAdd
BLAST
Transmembranei68 – 8821HelicalSequence analysisAdd
BLAST
Topological domaini89 – 10315PeriplasmicSequence analysisAdd
BLAST
Transmembranei104 – 12421HelicalSequence analysisAdd
BLAST
Topological domaini125 – 1273CytoplasmicSequence analysis
Transmembranei128 – 14821HelicalSequence analysisAdd
BLAST
Topological domaini149 – 17426PeriplasmicSequence analysisAdd
BLAST
Transmembranei175 – 19521HelicalSequence analysisAdd
BLAST
Topological domaini196 – 2027CytoplasmicSequence analysis
Transmembranei203 – 22321HelicalSequence analysisAdd
BLAST
Topological domaini224 – 2252PeriplasmicSequence analysis

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 225225Type 4 prepilin-like proteins leader peptide-processing enzymePRO_0000192632Add
BLAST

Proteomic databases

PaxDbiP25960.

Expressioni

Inductioni

Silenced by the DNA-binding protein H-NS under standard growth conditions.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4262465. 210 interactions.
STRINGi511145.b3335.

Structurei

3D structure databases

ProteinModelPortaliP25960.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A24 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EHH. Bacteria.
COG1989. LUCA.
HOGENOMiHOG000248584.
InParanoidiP25960.
KOiK02464.
OMAiNIMITMA.
OrthoDBiEOG6F55M8.
PhylomeDBiP25960.

Family and domain databases

InterProiIPR014032. Peptidase_A24A_bac.
IPR000045. Prepilin_IV_endopep_pep.
[Graphical view]
PfamiPF01478. Peptidase_A24. 1 hit.
[Graphical view]
PRINTSiPR00864. PREPILNPTASE.

Sequencei

Sequence statusi: Complete.

P25960-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMLLPLFIL VGFIADYFVN AIAYHLSPLE DKTALTFRQV LVHFRQKKYA
60 70 80 90 100
WHDTVPLILC VAAAIACALA PFTPIVTGAL FLYFCFVLTL SVIDFRTQLL
110 120 130 140 150
PDKLTLPLLW LGLVFNAQYG LIDLHDAVYG AVAGYGVLWC VYWGVWLVCH
160 170 180 190 200
KEGLGYGDFK LLAAAGAWCG WQTLPMILLI ASLGGIGYAI VSQLLQRRTI
210 220
TTIAFGPWLA LGSMINLGYL AWISY
Length:225
Mass (Da):24,957
Last modified:June 1, 1994 - v2
Checksum:i90297BB7E6592EDD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti131 – 1311A → P in AAC13988 (PubMed:2661540).Curated
Sequence conflicti208 – 2092WL → CV in AAA58132 (PubMed:9278503).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28106 Genomic DNA. Translation: AAC36928.1.
U18997 Genomic DNA. Translation: AAA58132.1.
U00096 Genomic DNA. Translation: AAC76360.1.
AP009048 Genomic DNA. Translation: BAE77956.1.
M27176 mRNA. Translation: AAC13988.1.
PIRiB65127.
RefSeqiNP_417794.1. NC_000913.3.
WP_000178154.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76360; AAC76360; b3335.
BAE77956; BAE77956; BAE77956.
GeneIDi947840.
KEGGiecj:JW3297.
eco:b3335.
PATRICi32122102. VBIEscCol129921_3428.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28106 Genomic DNA. Translation: AAC36928.1.
U18997 Genomic DNA. Translation: AAA58132.1.
U00096 Genomic DNA. Translation: AAC76360.1.
AP009048 Genomic DNA. Translation: BAE77956.1.
M27176 mRNA. Translation: AAC13988.1.
PIRiB65127.
RefSeqiNP_417794.1. NC_000913.3.
WP_000178154.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP25960.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262465. 210 interactions.
STRINGi511145.b3335.

Protein family/group databases

MEROPSiA24.A10.

Proteomic databases

PaxDbiP25960.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76360; AAC76360; b3335.
BAE77956; BAE77956; BAE77956.
GeneIDi947840.
KEGGiecj:JW3297.
eco:b3335.
PATRICi32122102. VBIEscCol129921_3428.

Organism-specific databases

EchoBASEiEB1334.
EcoGeneiEG11359. gspO.

Phylogenomic databases

eggNOGiENOG4105EHH. Bacteria.
COG1989. LUCA.
HOGENOMiHOG000248584.
InParanoidiP25960.
KOiK02464.
OMAiNIMITMA.
OrthoDBiEOG6F55M8.
PhylomeDBiP25960.

Enzyme and pathway databases

BioCyciEcoCyc:EG11359-MONOMER.
ECOL316407:JW3297-MONOMER.
MetaCyc:EG11359-MONOMER.

Miscellaneous databases

PROiP25960.

Family and domain databases

InterProiIPR014032. Peptidase_A24A_bac.
IPR000045. Prepilin_IV_endopep_pep.
[Graphical view]
PfamiPF01478. Peptidase_A24. 1 hit.
[Graphical view]
PRINTSiPR00864. PREPILNPTASE.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Escherichia coli contains a set of genes homologous to those involved in protein secretion, DNA uptake and the assembly of type-4 fimbriae in other bacteria."
    Whitchurch C.B., Mattick J.S.
    Gene 150:9-15(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Cloning, sequencing, and mapping of the bacterioferritin gene (bfr) of Escherichia coli K-12."
    Andrews S.C., Harrison P.M., Guest J.R.
    J. Bacteriol. 171:3940-3947(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 116-225.
    Strain: K12.
  5. "Characterisation of a Pseudomonas aeruginosa twitching motility gene and evidence for a specialised protein export system widespread in eubacteria."
    Whitchurch C.B., Hobbs M., Livingston S.P., Krishnapillai V., Mattick J.S.
    Gene 101:33-44(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  6. "The cryptic general secretory pathway (gsp) operon of Escherichia coli K-12 encodes functional proteins."
    Francetic O., Pugsley A.P.
    J. Bacteriol. 178:3544-3549(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: LACK OF EXPRESSION, GENE NAME.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  7. "Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion."
    Francetic O., Belin D., Badaut C., Pugsley A.P.
    EMBO J. 19:6697-6703(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: LACK OF EXPRESSION, TRANSCRIPTIONAL REGULATION.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  8. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiLEP4_ECOLI
AccessioniPrimary (citable) accession number: P25960
Secondary accession number(s): Q2M700
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: June 1, 1994
Last modified: January 20, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Part of a cryptic operon that encodes proteins involved in type II secretion machinery in other organisms, but is not expressed in strain K12. However, GspO is functional when expressed from a stronger promoter (PubMed:8655552).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.