Reviewed, UniProtKB/Swiss-Prot P25950 (MCEL_RFVKA)

Names and origin

Protein names

Recommended name:
    mRNA-capping enzyme large subunit
Including the following 2 domains:
    1- Recommended name:
            Polynucleotide 5'-triphosphatase
              EC=3.1.3.33
        Alternative name(s):
            mRNA 5'-triphosphatase
              Short name=TPase
    2- Recommended name:
            mRNA guanylyltransferase
              EC=2.7.7.50
        Alternative name(s):
            GTP--RNA guanylyltransferase
              Short name=GTase

Gene names

ORF Names:

D3R

Organism

Rabbit fibroma virus (strain Kasza) (RFV) (Shope fibroma virus (strain Kasza))

Taxonomic identifier

10272 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Leporipoxvirus

Virus host

Oryctolagus cuniculus (Rabbit) [TaxID: 9986]

Protein attributes

Sequence length	836 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the first two reactions in the mRNA cap formation pathway.
Catalytic activity	A 5'-phosphopolynucleotide + H2O = a polynucleotide + phosphate. GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
Subunit structure	Heterodimer of a large and a small subunit.
Sequence similarities	Belongs to the viral GTase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase Nucleotidyltransferase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA guanylyltransferase activity Inferred from electronic annotation. Source: EC polynucleotide 5'-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 836	836	mRNA-capping enzyme large subunit		PRO_0000210131
Sites
	Active site	256	1	N6-GMP-lysine intermediate Potential

Sequences

	Sequence		Length	Mass (Da)
	P25950-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 7375FDA548AE1730	FASTA	836	97,018
	10 20 30 40 50 60 MDDSKKKRGT DYIEELILLY EDVPNPVQTD DMNHEVELTF IQPPVITLST LLPFATSQES 70 80 90 100 110 120 YILFTVTNKG VKIRNRINLS KIHGLDLKNI QLVDSIDNII WEKKTLVKEH KIDSVALVKY 130 140 150 160 170 180 STEEKYIFLD YKKYLSAIKL ELVNVVQVKV KHVTVDFKFK YFLGSGAQAK SSLLHVLNHP 190 200 210 220 230 240 KSKPNPSLEF EIITTDEKID SASLRKELIA LFKLVFMASP SNIILDVVFK NPVQTILLKK 250 260 270 280 290 300 NELPGIDLTN LYVTTKTDGV GVLITVTNKG IYCFFTHLQY TIRYDTTFES NESVTLYGEA 310 320 330 340 350 360 VKQNNVWQIY LIKLITPKVS DRFKEKEYVE ERLQNICDRM TFKVKKYEGP FESHSEIIDL 370 380 390 400 410 420 LTTYLPSQPE GVVLFYSDQR NQPDYKIKLD NTTDHMINII YRYMSSEPVI FGENSTFLEY 430 440 450 460 470 480 KKFSDDKGFP KDYGTGKLML TDNVRYLNNI YCIAFTNVYE DVGIKNVVVP IKFISEFSAT 490 500 510 520 530 540 GELIKPRIDK TFKYLYKEYY GNQYQIVVAH IRDQNIKIGD VLDEDKLSDV GQHYANDKYR 550 560 570 580 590 600 LNPDVSYFTN KRTRGPLGIL SNYVKTLLIS LYCSKTFLDN SNKRKVLAID FGNGADLEKY 610 620 630 640 650 660 FYGEISSLVA TDPDKEAIGR CIERYNSLNS GIKSKYYKFD YIQETIRSVT YVSSVREVFF 670 680 690 700 710 720 FGKFDLVDWQ FAIHYSFHPK HYATVMNNLT ELTASGGKVL ITTMDGDLLS QLTDKKTFVI 730 740 750 760 770 780 HKNLPSSENY MSVEKIHEDQ ILVYNPSSMS RPMQEYIVKR VNLTKIFSEY GFELIDCVHF 790 800 810 820 830 DTIIERSKRF INSVSKMEER KSTKNFFELN REALKHEGTD IDDLLRYYIV YVFSKR

References

[1]	"Identification and DNA sequence of the large subunit of the capping enzyme from Shope fibroma virus." Upton C., Stuart D., McFadden G. Virology 183:773-777(1991) [PubMed: 1649507] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M63902 Genomic DNA. Translation: AAA47224.1.
PIR	QQVZRA. A40478.
RefSeq	NP_051965.1.
3D structure databases
SMR	P25950. Positions 537-836.
ModBase	Search...
Genome annotation databases
GeneID	1486919.
Family and domain databases
InterPro	IPR019602. mRNA_cap_ATPase/GuylTrfase_vir. IPR004971. Pox_MCEL. [Graphical view]
Pfam	PF10640. Pox_ATPase-GT. 1 hit. PF03291. Pox_MCEL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCEL_RFVKA

Accession

Primary (citable) accession number: P25950

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	January 19, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)