Regulatory protein AfsR - Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Regulatory protein AfsR

Gene names

Name:	afsR
Synonyms:	afsB
Ordered Locus Names:	SCO4426
ORF Names:	SCD6.04c

Organism

Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) [Reference proteome] [HAMAP]

Taxonomic identifier

100226 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces ›

Protein attributes

Sequence length	993 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Global regulatory protein for secondary metabolite formation.
Domain	The N-terminal and the C-terminal regions are independently capable of directing actinorhodin production.
Post-translational modification	Phosphorylated by AfsK.
Sequence similarities	Belongs to the AfsR/DnrI/RedD regulatory family. Contains 1 NB-ARC domain. Contains 3 TPR repeats.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis Transcription Transcription regulation Two-component regulatory system
Domain	Repeat TPR repeat
Ligand	ATP-binding DNA-binding Nucleotide-binding Pigment
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: InterPro intracellular signal transduction Inferred from electronic annotation. Source: GOC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	ADP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW phosphorelay response regulator activity Inferred from electronic annotation. Source: InterPro pigment binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 993

993

Regulatory protein AfsR

PRO_0000110004

Regions

Domain

280 – 579

300

NB-ARC

Repeat

823 – 856

34

TPR 1

Repeat

863 – 896

34

TPR 2

Repeat

942 – 975

34

TPR 3

Nucleotide binding

330 – 337

8

ATP Potential

DNA binding

796 – 811

16

H-T-H motif Potential

DNA binding

974 – 988

15

H-T-H motif Potential

Experimental info

Sequence conflict

256

1

Q → R in BAA14186. Ref.1

Sequence conflict

318

1

A → P in BAA14186. Ref.1

Sequence conflict

751 – 766

16

DVLLV…AEEEA → MCSWCPVASSMPRKRH Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

P25941 [UniParc].

Last modified October 18, 2001. Version 2.
Checksum: 8793CF27CEA7254C
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FASTA

993

105,716

        10         20         30         40         50         60 
MDGGPRVPEQ RRPGFPAEEE ESGALRFGVL GPVRAWRDGE TLATGSPQQR ALLAALLLRE 

        70         80         90        100        110        120 
GRTATAGELI DALWGEEPPS QALAAVRTYA SRLRKVLDPG VLVSESGGYA VRGLAEGALD 

       130        140        150        160        170        180 
LARAQDLASA AEKARSAGDL CHARDLLRRA LDLWDGEVLA GVPGPYAQTQ RVRLGEWRLQ 

       190        200        210        220        230        240 
LLETRLDMDL DQGCHAEAVS ELTALTAAHP LRERLRELLM LALYRSGRQA EALAVYADTR 

       250        260        270        280        290        300 
RLLADELGVD PRPGLQELQQ RILQADPALA ELSAATAETA TATLRPAQLP ATVSDFTGRA 

       310        320        330        340        350        360 
AFVRELSDVL SAASGESASG RVMAVSALAG IGGVGKTTLA VHVAHRARAA FPDGQLYVDL 

       370        380        390        400        410        420 
QGAGARPAEP ETVLGSFLRA LGTADSAIPD SLEERAALYR SVLDGRRVLV LLDNARDAAQ 

       430        440        450        460        470        480 
VRPLLPGTDG CAALVTARVR MVDLAGAHLV DLDVMAPEEA LALFTKIVGE ERVASERQAA 

       490        500        510        520        530        540 
LDVVGACGFL PLAIRIAASR LAARRTWTVS VLAAKLADER RRLDELQAGD QAVEATFELG 

       550        560        570        580        590        600 
YGQLEPAQAR AFRLLGLADG PDISLAAAAA VLDLPAQDTE DLLESLVDTS LLESAAPGRY 

       610        620        630        640        650        660 
RFHDLVRLYA RACAERTERD GNAPSERGAA LSRLLDFYLA TAAGVYAIER PGDRLVDGLE 

       670        680        690        700        710        720 
PTEYPGLTFT EGSAALDWLY TEAAPLLACV RQSAGTARLR RAVDLLWAAK DLTESGANSH 

       730        740        750        760        770        780 
QYEATARAMC DATGSAADTR AEGRARTVLS DVLLVSGRIE HAEEEARLAM RLAGSAEDSA 

       790        800        810        820        830        840 
AVSWVANNRG LVCLHQRRYA EGKGLFHQAI AGFRATDNRA GEASALSNLS RAQLGMGNVA 

       850        860        870        880        890        900 
EAVDIARQGL AVYAELGRTM RLANGHFALG VALTRAGRHE EALGEFAEAL DLFGDHRQRL 

       910        920        930        940        950        960 
WEGATNFRLA EVHLAAGRPS SAAQHAEQAL ALGCIGGDRM RGNVLALLGR ALSALGQADR 

       970        980        990 
ARACWREALS LYEQHDAQEV GEVRALLARS VAR

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure of AfsR, a global regulatory protein for secondary metabolite formation in Streptomyces coelicolor A3(2)." Horinouchi S., Kito M., Nishiyama M., Furuya K., Hong S.-K., Miyake K., Beppu T. Gene 95:49-56(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A3(2) / NRRL B-16638.
[2]	"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. Hopwood D.A. Nature 417:141-147(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.
[3]	"Nucleotide sequence of afsB, a pleiotropic gene involved in secondary metabolism in Streptomyces coelicolor A3(2) and 'Streptomyces lividans'." Horinouchi S., Suzuki H., Beppu T. J. Bacteriol. 168:257-269(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 751-993. Strain: A3(2) / NRRL B-16638.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D90155 Genomic DNA. Translation: BAA14186.1. AL939120 Genomic DNA. Translation: CAB88433.1. M16011 Genomic DNA. Translation: AAA26694.1.
PIR	A25037. JQ0488.
RefSeq	NP_628593.1. NC_003888.3.
3D structure databases
ProteinModelPortal	P25941.
ModBase	Search...
Protein-protein interaction databases
STRING	100226.SCO4426.
PTM databases
PhosSite	P010470.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAB88433; CAB88433; CAB88433.
GeneID	1099866.
KEGG	sco:SCO4426.
PATRIC	23738662. VBIStrCoe124346_4495.
Phylogenomic databases
eggNOG	COG3629.
HOGENOM	HOG000253135.
OMA	MMEREEG.
ProtClustDB	CLSK635966.
Family and domain databases
Gene3D	1.10.10.10. 1 hit. 1.25.40.10. 2 hits.
InterPro	IPR005158. BTAD. IPR000767. Disease_R. IPR002182. NB-ARC. IPR001867. Sig_transdc_resp-reg_C. IPR016032. Sig_transdc_resp-reg_C-effctor. IPR013026. TPR-contain_dom. IPR011990. TPR-like_helical. IPR013105. TPR_2. IPR019734. TPR_repeat. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF03704. BTAD. 1 hit. PF00931. NB-ARC. 1 hit. PF07719. TPR_2. 1 hit. PF00486. Trans_reg_C. 1 hit. [Graphical view]
PRINTS	PR00364. DISEASERSIST.
SMART	SM01043. BTAD. 1 hit. SM00028. TPR. 3 hits. SM00862. Trans_reg_C. 1 hit. [Graphical view]
SUPFAM	SSF46894. Bipartite_resp_reg_C-effector. 1 hit.
PROSITE	PS50005. TPR. 3 hits. PS50293. TPR_REGION. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AFSR_STRCO

Accession

Primary (citable) accession number: P25941
Secondary accession number(s): P08467, Q9L000

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	October 18, 2001
Last modified:	May 1, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents