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P25941

- AFSR_STRCO

UniProt

P25941 - AFSR_STRCO

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Protein

Regulatory protein AfsR

Gene

afsR

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Global regulatory protein for secondary metabolite formation.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi330 – 3378ATPSequence Analysis
DNA bindingi796 – 81116H-T-H motifSequence AnalysisAdd
BLAST
DNA bindingi974 – 98815H-T-H motifSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. ADP binding Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. DNA binding Source: UniProtKB-KW
  4. pigment binding Source: UniProtKB-KW

GO - Biological processi

  1. antibiotic biosynthetic process Source: UniProtKB-KW
  2. phosphorelay signal transduction system Source: UniProtKB-KW
  3. regulation of transcription, DNA-templated Source: UniProtKB-KW
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Antibiotic biosynthesis, Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding, Pigment

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein AfsR
Gene namesi
Name:afsR
Synonyms:afsB
Ordered Locus Names:SCO4426
ORF Names:SCD6.04c
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 993993Regulatory protein AfsRPRO_0000110004Add
BLAST

Post-translational modificationi

Phosphorylated by AfsK.

Keywords - PTMi

Phosphoprotein

PTM databases

PhosSiteiP010470.

Interactioni

Protein-protein interaction databases

STRINGi100226.SCO4426.

Structurei

3D structure databases

ProteinModelPortaliP25941.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini280 – 579300NB-ARCAdd
BLAST
Repeati823 – 85634TPR 1Add
BLAST
Repeati863 – 89634TPR 2Add
BLAST
Repeati942 – 97534TPR 3Add
BLAST

Domaini

The N-terminal and the C-terminal regions are independently capable of directing actinorhodin production.

Sequence similaritiesi

Belongs to the AfsR/DnrI/RedD regulatory family.Curated
Contains 1 NB-ARC domain.Curated
Contains 3 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiCOG3629.
HOGENOMiHOG000253135.
InParanoidiP25941.
OMAiMMEREEG.
OrthoDBiEOG6PP9HC.
PhylomeDBiP25941.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.25.40.10. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR005158. BTAD.
IPR002182. NB-ARC.
IPR027417. P-loop_NTPase.
IPR001867. Sig_transdc_resp-reg_C.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR013105. TPR_2.
IPR019734. TPR_repeat.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF03704. BTAD. 1 hit.
PF00931. NB-ARC. 1 hit.
PF07719. TPR_2. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
SMARTiSM01043. BTAD. 1 hit.
SM00028. TPR. 3 hits.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50005. TPR. 3 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25941-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDGGPRVPEQ RRPGFPAEEE ESGALRFGVL GPVRAWRDGE TLATGSPQQR
60 70 80 90 100
ALLAALLLRE GRTATAGELI DALWGEEPPS QALAAVRTYA SRLRKVLDPG
110 120 130 140 150
VLVSESGGYA VRGLAEGALD LARAQDLASA AEKARSAGDL CHARDLLRRA
160 170 180 190 200
LDLWDGEVLA GVPGPYAQTQ RVRLGEWRLQ LLETRLDMDL DQGCHAEAVS
210 220 230 240 250
ELTALTAAHP LRERLRELLM LALYRSGRQA EALAVYADTR RLLADELGVD
260 270 280 290 300
PRPGLQELQQ RILQADPALA ELSAATAETA TATLRPAQLP ATVSDFTGRA
310 320 330 340 350
AFVRELSDVL SAASGESASG RVMAVSALAG IGGVGKTTLA VHVAHRARAA
360 370 380 390 400
FPDGQLYVDL QGAGARPAEP ETVLGSFLRA LGTADSAIPD SLEERAALYR
410 420 430 440 450
SVLDGRRVLV LLDNARDAAQ VRPLLPGTDG CAALVTARVR MVDLAGAHLV
460 470 480 490 500
DLDVMAPEEA LALFTKIVGE ERVASERQAA LDVVGACGFL PLAIRIAASR
510 520 530 540 550
LAARRTWTVS VLAAKLADER RRLDELQAGD QAVEATFELG YGQLEPAQAR
560 570 580 590 600
AFRLLGLADG PDISLAAAAA VLDLPAQDTE DLLESLVDTS LLESAAPGRY
610 620 630 640 650
RFHDLVRLYA RACAERTERD GNAPSERGAA LSRLLDFYLA TAAGVYAIER
660 670 680 690 700
PGDRLVDGLE PTEYPGLTFT EGSAALDWLY TEAAPLLACV RQSAGTARLR
710 720 730 740 750
RAVDLLWAAK DLTESGANSH QYEATARAMC DATGSAADTR AEGRARTVLS
760 770 780 790 800
DVLLVSGRIE HAEEEARLAM RLAGSAEDSA AVSWVANNRG LVCLHQRRYA
810 820 830 840 850
EGKGLFHQAI AGFRATDNRA GEASALSNLS RAQLGMGNVA EAVDIARQGL
860 870 880 890 900
AVYAELGRTM RLANGHFALG VALTRAGRHE EALGEFAEAL DLFGDHRQRL
910 920 930 940 950
WEGATNFRLA EVHLAAGRPS SAAQHAEQAL ALGCIGGDRM RGNVLALLGR
960 970 980 990
ALSALGQADR ARACWREALS LYEQHDAQEV GEVRALLARS VAR
Length:993
Mass (Da):105,716
Last modified:October 18, 2001 - v2
Checksum:i8793CF27CEA7254C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti256 – 2561Q → R in BAA14186. (PubMed:2253887)Curated
Sequence conflicti318 – 3181A → P in BAA14186. (PubMed:2253887)Curated
Sequence conflicti751 – 76616DVLLV…AEEEA → MCSWCPVASSMPRKRH(PubMed:2428809)CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90155 Genomic DNA. Translation: BAA14186.1.
AL939120 Genomic DNA. Translation: CAB88433.1.
M16011 Genomic DNA. Translation: AAA26694.1.
PIRiA25037.
JQ0488.
RefSeqiNP_628593.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAB88433; CAB88433; CAB88433.
GeneIDi1099866.
KEGGisco:SCO4426.
PATRICi23738662. VBIStrCoe124346_4495.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90155 Genomic DNA. Translation: BAA14186.1 .
AL939120 Genomic DNA. Translation: CAB88433.1 .
M16011 Genomic DNA. Translation: AAA26694.1 .
PIRi A25037.
JQ0488.
RefSeqi NP_628593.1. NC_003888.3.

3D structure databases

ProteinModelPortali P25941.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 100226.SCO4426.

PTM databases

PhosSitei P010470.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB88433 ; CAB88433 ; CAB88433 .
GeneIDi 1099866.
KEGGi sco:SCO4426.
PATRICi 23738662. VBIStrCoe124346_4495.

Phylogenomic databases

eggNOGi COG3629.
HOGENOMi HOG000253135.
InParanoidi P25941.
OMAi MMEREEG.
OrthoDBi EOG6PP9HC.
PhylomeDBi P25941.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
1.25.40.10. 2 hits.
3.40.50.300. 1 hit.
InterProi IPR005158. BTAD.
IPR002182. NB-ARC.
IPR027417. P-loop_NTPase.
IPR001867. Sig_transdc_resp-reg_C.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR013105. TPR_2.
IPR019734. TPR_repeat.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF03704. BTAD. 1 hit.
PF00931. NB-ARC. 1 hit.
PF07719. TPR_2. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view ]
SMARTi SM01043. BTAD. 1 hit.
SM00028. TPR. 3 hits.
SM00862. Trans_reg_C. 1 hit.
[Graphical view ]
SUPFAMi SSF46894. SSF46894. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEi PS50005. TPR. 3 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Primary structure of AfsR, a global regulatory protein for secondary metabolite formation in Streptomyces coelicolor A3(2)."
    Horinouchi S., Kito M., Nishiyama M., Furuya K., Hong S.-K., Miyake K., Beppu T.
    Gene 95:49-56(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: A3(2) / NRRL B-16638.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.
  3. "Nucleotide sequence of afsB, a pleiotropic gene involved in secondary metabolism in Streptomyces coelicolor A3(2) and 'Streptomyces lividans'."
    Horinouchi S., Suzuki H., Beppu T.
    J. Bacteriol. 168:257-269(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 751-993.
    Strain: A3(2) / NRRL B-16638.

Entry informationi

Entry nameiAFSR_STRCO
AccessioniPrimary (citable) accession number: P25941
Secondary accession number(s): P08467, Q9L000
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: October 18, 2001
Last modified: November 26, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3