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P25941 (AFSR_STRCO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Regulatory protein AfsR
Gene names
Name:afsR
Synonyms:afsB
Ordered Locus Names:SCO4426
ORF Names:SCD6.04c
OrganismStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) [Reference proteome] [HAMAP]
Taxonomic identifier100226 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length993 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Global regulatory protein for secondary metabolite formation.

Domain

The N-terminal and the C-terminal regions are independently capable of directing actinorhodin production.

Post-translational modification

Phosphorylated by AfsK.

Sequence similarities

Belongs to the AfsR/DnrI/RedD regulatory family.

Contains 1 NB-ARC domain.

Contains 3 TPR repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 993993Regulatory protein AfsR
PRO_0000110004

Regions

Domain280 – 579300NB-ARC
Repeat823 – 85634TPR 1
Repeat863 – 89634TPR 2
Repeat942 – 97534TPR 3
Nucleotide binding330 – 3378ATP Potential
DNA binding796 – 81116H-T-H motif Potential
DNA binding974 – 98815H-T-H motif Potential

Experimental info

Sequence conflict2561Q → R in BAA14186. Ref.1
Sequence conflict3181A → P in BAA14186. Ref.1
Sequence conflict751 – 76616DVLLV…AEEEA → MCSWCPVASSMPRKRH Ref.3

Sequences

Sequence LengthMass (Da)Tools
P25941 [UniParc].

Last modified October 18, 2001. Version 2.
Checksum: 8793CF27CEA7254C

FASTA993105,716
        10         20         30         40         50         60 
MDGGPRVPEQ RRPGFPAEEE ESGALRFGVL GPVRAWRDGE TLATGSPQQR ALLAALLLRE 

        70         80         90        100        110        120 
GRTATAGELI DALWGEEPPS QALAAVRTYA SRLRKVLDPG VLVSESGGYA VRGLAEGALD 

       130        140        150        160        170        180 
LARAQDLASA AEKARSAGDL CHARDLLRRA LDLWDGEVLA GVPGPYAQTQ RVRLGEWRLQ 

       190        200        210        220        230        240 
LLETRLDMDL DQGCHAEAVS ELTALTAAHP LRERLRELLM LALYRSGRQA EALAVYADTR 

       250        260        270        280        290        300 
RLLADELGVD PRPGLQELQQ RILQADPALA ELSAATAETA TATLRPAQLP ATVSDFTGRA 

       310        320        330        340        350        360 
AFVRELSDVL SAASGESASG RVMAVSALAG IGGVGKTTLA VHVAHRARAA FPDGQLYVDL 

       370        380        390        400        410        420 
QGAGARPAEP ETVLGSFLRA LGTADSAIPD SLEERAALYR SVLDGRRVLV LLDNARDAAQ 

       430        440        450        460        470        480 
VRPLLPGTDG CAALVTARVR MVDLAGAHLV DLDVMAPEEA LALFTKIVGE ERVASERQAA 

       490        500        510        520        530        540 
LDVVGACGFL PLAIRIAASR LAARRTWTVS VLAAKLADER RRLDELQAGD QAVEATFELG 

       550        560        570        580        590        600 
YGQLEPAQAR AFRLLGLADG PDISLAAAAA VLDLPAQDTE DLLESLVDTS LLESAAPGRY 

       610        620        630        640        650        660 
RFHDLVRLYA RACAERTERD GNAPSERGAA LSRLLDFYLA TAAGVYAIER PGDRLVDGLE 

       670        680        690        700        710        720 
PTEYPGLTFT EGSAALDWLY TEAAPLLACV RQSAGTARLR RAVDLLWAAK DLTESGANSH 

       730        740        750        760        770        780 
QYEATARAMC DATGSAADTR AEGRARTVLS DVLLVSGRIE HAEEEARLAM RLAGSAEDSA 

       790        800        810        820        830        840 
AVSWVANNRG LVCLHQRRYA EGKGLFHQAI AGFRATDNRA GEASALSNLS RAQLGMGNVA 

       850        860        870        880        890        900 
EAVDIARQGL AVYAELGRTM RLANGHFALG VALTRAGRHE EALGEFAEAL DLFGDHRQRL 

       910        920        930        940        950        960 
WEGATNFRLA EVHLAAGRPS SAAQHAEQAL ALGCIGGDRM RGNVLALLGR ALSALGQADR 

       970        980        990 
ARACWREALS LYEQHDAQEV GEVRALLARS VAR 

« Hide

References

« Hide 'large scale' references
[1]"Primary structure of AfsR, a global regulatory protein for secondary metabolite formation in Streptomyces coelicolor A3(2)."
Horinouchi S., Kito M., Nishiyama M., Furuya K., Hong S.-K., Miyake K., Beppu T.
Gene 95:49-56(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: A3(2) / NRRL B-16638.
[2]"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)."
Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. expand/collapse author list , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.
Nature 417:141-147(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-471 / A3(2) / M145.
[3]"Nucleotide sequence of afsB, a pleiotropic gene involved in secondary metabolism in Streptomyces coelicolor A3(2) and 'Streptomyces lividans'."
Horinouchi S., Suzuki H., Beppu T.
J. Bacteriol. 168:257-269(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 751-993.
Strain: A3(2) / NRRL B-16638.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D90155 Genomic DNA. Translation: BAA14186.1.
AL939120 Genomic DNA. Translation: CAB88433.1.
M16011 Genomic DNA. Translation: AAA26694.1.
PIRA25037.
JQ0488.
RefSeqNP_628593.1. NC_003888.3.

3D structure databases

ProteinModelPortalP25941.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING100226.SCO4426.

PTM databases

PhosSiteP010470.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB88433; CAB88433; CAB88433.
GeneID1099866.
KEGGsco:SCO4426.
PATRIC23738662. VBIStrCoe124346_4495.

Phylogenomic databases

eggNOGCOG3629.
HOGENOMHOG000253135.
OMAMMEREEG.
OrthoDBEOG6PP9HC.
PhylomeDBP25941.
ProtClustDBCLSK635966.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
1.25.40.10. 2 hits.
3.40.50.300. 1 hit.
InterProIPR005158. BTAD.
IPR000767. Disease_R.
IPR002182. NB-ARC.
IPR027417. P-loop_NTPase.
IPR001867. Sig_transdc_resp-reg_C.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical.
IPR013105. TPR_2.
IPR019734. TPR_repeat.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF03704. BTAD. 1 hit.
PF00931. NB-ARC. 1 hit.
PF07719. TPR_2. 1 hit.
PF00486. Trans_reg_C. 1 hit.
[Graphical view]
PRINTSPR00364. DISEASERSIST.
SMARTSM01043. BTAD. 1 hit.
SM00028. TPR. 3 hits.
SM00862. Trans_reg_C. 1 hit.
[Graphical view]
SUPFAMSSF46894. SSF46894. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEPS50005. TPR. 3 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAFSR_STRCO
AccessionPrimary (citable) accession number: P25941
Secondary accession number(s): P08467, Q9L000
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: October 18, 2001
Last modified: April 16, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families