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P25941

- AFSR_STRCO

UniProt

P25941 - AFSR_STRCO

Protein

Regulatory protein AfsR

Gene

afsR

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (18 Oct 2001)
      Previous versions | rss
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    Functioni

    Global regulatory protein for secondary metabolite formation.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi330 – 3378ATPSequence Analysis
    DNA bindingi796 – 81116H-T-H motifSequence AnalysisAdd
    BLAST
    DNA bindingi974 – 98815H-T-H motifSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. ADP binding Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. DNA binding Source: UniProtKB-KW
    4. phosphorelay response regulator activity Source: InterPro
    5. pigment binding Source: UniProtKB-KW

    GO - Biological processi

    1. antibiotic biosynthetic process Source: UniProtKB-KW
    2. defense response Source: InterPro
    3. regulation of transcription, DNA-templated Source: UniProtKB-KW
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Antibiotic biosynthesis, Transcription, Transcription regulation, Two-component regulatory system

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding, Pigment

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Regulatory protein AfsR
    Gene namesi
    Name:afsR
    Synonyms:afsB
    Ordered Locus Names:SCO4426
    ORF Names:SCD6.04c
    OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
    Taxonomic identifieri100226 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
    ProteomesiUP000001973: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 993993Regulatory protein AfsRPRO_0000110004Add
    BLAST

    Post-translational modificationi

    Phosphorylated by AfsK.

    Keywords - PTMi

    Phosphoprotein

    PTM databases

    PhosSiteiP010470.

    Interactioni

    Protein-protein interaction databases

    STRINGi100226.SCO4426.

    Structurei

    3D structure databases

    ProteinModelPortaliP25941.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini280 – 579300NB-ARCAdd
    BLAST
    Repeati823 – 85634TPR 1Add
    BLAST
    Repeati863 – 89634TPR 2Add
    BLAST
    Repeati942 – 97534TPR 3Add
    BLAST

    Domaini

    The N-terminal and the C-terminal regions are independently capable of directing actinorhodin production.

    Sequence similaritiesi

    Belongs to the AfsR/DnrI/RedD regulatory family.Curated
    Contains 1 NB-ARC domain.Curated
    Contains 3 TPR repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, TPR repeat

    Phylogenomic databases

    eggNOGiCOG3629.
    HOGENOMiHOG000253135.
    OMAiMMEREEG.
    OrthoDBiEOG6PP9HC.
    PhylomeDBiP25941.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    1.25.40.10. 2 hits.
    3.40.50.300. 1 hit.
    InterProiIPR005158. BTAD.
    IPR000767. Disease_R.
    IPR002182. NB-ARC.
    IPR027417. P-loop_NTPase.
    IPR001867. Sig_transdc_resp-reg_C.
    IPR016032. Sig_transdc_resp-reg_C-effctor.
    IPR013026. TPR-contain_dom.
    IPR011990. TPR-like_helical.
    IPR013105. TPR_2.
    IPR019734. TPR_repeat.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF03704. BTAD. 1 hit.
    PF00931. NB-ARC. 1 hit.
    PF07719. TPR_2. 1 hit.
    PF00486. Trans_reg_C. 1 hit.
    [Graphical view]
    PRINTSiPR00364. DISEASERSIST.
    SMARTiSM01043. BTAD. 1 hit.
    SM00028. TPR. 3 hits.
    SM00862. Trans_reg_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF46894. SSF46894. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS50005. TPR. 3 hits.
    PS50293. TPR_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25941-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDGGPRVPEQ RRPGFPAEEE ESGALRFGVL GPVRAWRDGE TLATGSPQQR    50
    ALLAALLLRE GRTATAGELI DALWGEEPPS QALAAVRTYA SRLRKVLDPG 100
    VLVSESGGYA VRGLAEGALD LARAQDLASA AEKARSAGDL CHARDLLRRA 150
    LDLWDGEVLA GVPGPYAQTQ RVRLGEWRLQ LLETRLDMDL DQGCHAEAVS 200
    ELTALTAAHP LRERLRELLM LALYRSGRQA EALAVYADTR RLLADELGVD 250
    PRPGLQELQQ RILQADPALA ELSAATAETA TATLRPAQLP ATVSDFTGRA 300
    AFVRELSDVL SAASGESASG RVMAVSALAG IGGVGKTTLA VHVAHRARAA 350
    FPDGQLYVDL QGAGARPAEP ETVLGSFLRA LGTADSAIPD SLEERAALYR 400
    SVLDGRRVLV LLDNARDAAQ VRPLLPGTDG CAALVTARVR MVDLAGAHLV 450
    DLDVMAPEEA LALFTKIVGE ERVASERQAA LDVVGACGFL PLAIRIAASR 500
    LAARRTWTVS VLAAKLADER RRLDELQAGD QAVEATFELG YGQLEPAQAR 550
    AFRLLGLADG PDISLAAAAA VLDLPAQDTE DLLESLVDTS LLESAAPGRY 600
    RFHDLVRLYA RACAERTERD GNAPSERGAA LSRLLDFYLA TAAGVYAIER 650
    PGDRLVDGLE PTEYPGLTFT EGSAALDWLY TEAAPLLACV RQSAGTARLR 700
    RAVDLLWAAK DLTESGANSH QYEATARAMC DATGSAADTR AEGRARTVLS 750
    DVLLVSGRIE HAEEEARLAM RLAGSAEDSA AVSWVANNRG LVCLHQRRYA 800
    EGKGLFHQAI AGFRATDNRA GEASALSNLS RAQLGMGNVA EAVDIARQGL 850
    AVYAELGRTM RLANGHFALG VALTRAGRHE EALGEFAEAL DLFGDHRQRL 900
    WEGATNFRLA EVHLAAGRPS SAAQHAEQAL ALGCIGGDRM RGNVLALLGR 950
    ALSALGQADR ARACWREALS LYEQHDAQEV GEVRALLARS VAR 993
    Length:993
    Mass (Da):105,716
    Last modified:October 18, 2001 - v2
    Checksum:i8793CF27CEA7254C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti256 – 2561Q → R in BAA14186. (PubMed:2253887)Curated
    Sequence conflicti318 – 3181A → P in BAA14186. (PubMed:2253887)Curated
    Sequence conflicti751 – 76616DVLLV…AEEEA → MCSWCPVASSMPRKRH(PubMed:2428809)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D90155 Genomic DNA. Translation: BAA14186.1.
    AL939120 Genomic DNA. Translation: CAB88433.1.
    M16011 Genomic DNA. Translation: AAA26694.1.
    PIRiA25037.
    JQ0488.
    RefSeqiNP_628593.1. NC_003888.3.
    WP_011029645.1. NC_003888.3.

    Genome annotation databases

    EnsemblBacteriaiCAB88433; CAB88433; CAB88433.
    GeneIDi1099866.
    KEGGisco:SCO4426.
    PATRICi23738662. VBIStrCoe124346_4495.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D90155 Genomic DNA. Translation: BAA14186.1 .
    AL939120 Genomic DNA. Translation: CAB88433.1 .
    M16011 Genomic DNA. Translation: AAA26694.1 .
    PIRi A25037.
    JQ0488.
    RefSeqi NP_628593.1. NC_003888.3.
    WP_011029645.1. NC_003888.3.

    3D structure databases

    ProteinModelPortali P25941.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 100226.SCO4426.

    PTM databases

    PhosSitei P010470.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB88433 ; CAB88433 ; CAB88433 .
    GeneIDi 1099866.
    KEGGi sco:SCO4426.
    PATRICi 23738662. VBIStrCoe124346_4495.

    Phylogenomic databases

    eggNOGi COG3629.
    HOGENOMi HOG000253135.
    OMAi MMEREEG.
    OrthoDBi EOG6PP9HC.
    PhylomeDBi P25941.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    1.25.40.10. 2 hits.
    3.40.50.300. 1 hit.
    InterProi IPR005158. BTAD.
    IPR000767. Disease_R.
    IPR002182. NB-ARC.
    IPR027417. P-loop_NTPase.
    IPR001867. Sig_transdc_resp-reg_C.
    IPR016032. Sig_transdc_resp-reg_C-effctor.
    IPR013026. TPR-contain_dom.
    IPR011990. TPR-like_helical.
    IPR013105. TPR_2.
    IPR019734. TPR_repeat.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF03704. BTAD. 1 hit.
    PF00931. NB-ARC. 1 hit.
    PF07719. TPR_2. 1 hit.
    PF00486. Trans_reg_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00364. DISEASERSIST.
    SMARTi SM01043. BTAD. 1 hit.
    SM00028. TPR. 3 hits.
    SM00862. Trans_reg_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46894. SSF46894. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS50005. TPR. 3 hits.
    PS50293. TPR_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of AfsR, a global regulatory protein for secondary metabolite formation in Streptomyces coelicolor A3(2)."
      Horinouchi S., Kito M., Nishiyama M., Furuya K., Hong S.-K., Miyake K., Beppu T.
      Gene 95:49-56(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: A3(2) / NRRL B-16638.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-471 / A3(2) / M145.
    3. "Nucleotide sequence of afsB, a pleiotropic gene involved in secondary metabolism in Streptomyces coelicolor A3(2) and 'Streptomyces lividans'."
      Horinouchi S., Suzuki H., Beppu T.
      J. Bacteriol. 168:257-269(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 751-993.
      Strain: A3(2) / NRRL B-16638.

    Entry informationi

    Entry nameiAFSR_STRCO
    AccessioniPrimary (citable) accession number: P25941
    Secondary accession number(s): P08467, Q9L000
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: October 18, 2001
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3