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P25916

- BMI1_MOUSE

UniProt

P25916 - BMI1_MOUSE

Protein

Polycomb complex protein BMI-1

Gene

Bmi1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    Functioni

    Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2 By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri18 – 5740RING-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. protein binding Source: IntAct
    3. sequence-specific DNA binding Source: MGI
    4. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. brain development Source: MGI
    2. cellular process Source: MGI
    3. DNA methylation Source: MGI
    4. embryonic skeletal system development Source: MGI
    5. embryonic skeletal system morphogenesis Source: MGI
    6. histone acetylation Source: MGI
    7. histone ubiquitination Source: MGI
    8. humoral immune response Source: MGI
    9. in utero embryonic development Source: MGI
    10. negative regulation of apoptotic signaling pathway Source: MGI
    11. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    12. positive regulation of B cell proliferation Source: HGNC
    13. positive regulation of immature T cell proliferation in thymus Source: HGNC
    14. positive regulation of ubiquitin-protein transferase activity Source: UniProtKB
    15. rostrocaudal neural tube patterning Source: MGI
    16. skeletal system development Source: MGI
    17. somatic stem cell division Source: MGI
    18. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chromatin regulator, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_188970. Oxidative Stress Induced Senescence.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polycomb complex protein BMI-1
    Alternative name(s):
    Polycomb group RING finger protein 4
    Gene namesi
    Name:Bmi1
    Synonyms:Bmi-1, Pcgf4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:88174. Bmi1.

    Subcellular locationi

    Nucleus. Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. heterochromatin Source: MGI
    3. nuclear body Source: MGI
    4. nucleus Source: MGI
    5. PcG protein complex Source: MGI
    6. ubiquitin ligase complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Keywords - Diseasei

    Proto-oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 324324Polycomb complex protein BMI-1PRO_0000055988Add
    BLAST

    Post-translational modificationi

    May be polyubiquitinated; which does not lead to proteasomal degradation By similarity. Monoubiquitinated.By similarity1 Publication

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    PaxDbiP25916.
    PRIDEiP25916.

    PTM databases

    PhosphoSiteiP25916.

    Expressioni

    Tissue specificityi

    Detected in most organs with high expression levels in thymus, heart, brain and testis.

    Gene expression databases

    ArrayExpressiP25916.
    BgeeiP25916.
    CleanExiMM_BMI1.
    GenevestigatoriP25916.

    Interactioni

    Subunit structurei

    Component of a PRC1-like complex. Interacts with RING1 and RING2. Interacts vwith CBX7 and CBX8. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Cbx8Q9QXV13EBI-927401,EBI-1216641
    PHC2Q8IXK02EBI-927401,EBI-713786From a different organism.
    Phc2Q9QWH13EBI-927401,EBI-642357
    Ring1O357304EBI-927401,EBI-929310
    Rnf2Q9CQJ411EBI-927401,EBI-927321

    Protein-protein interaction databases

    BioGridi198359. 27 interactions.
    DIPiDIP-132N.
    IntActiP25916. 17 interactions.

    Structurei

    Secondary structure

    1
    324
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi9 – 124
    Helixi13 – 153
    Turni19 – 213
    Beta strandi22 – 243
    Beta strandi26 – 316
    Turni32 – 343
    Beta strandi37 – 393
    Helixi40 – 478
    Turni54 – 563
    Helixi65 – 684
    Beta strandi69 – 713
    Helixi73 – 8210
    Helixi86 – 10015

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2CKLX-ray2.00A1-108[»]
    ProteinModelPortaliP25916.
    SMRiP25916. Positions 6-103.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP25916.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni162 – 22665Interaction with E4F1By similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi81 – 9515Nuclear localization signalSequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi249 – 32476Pro/Ser-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri18 – 5740RING-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG304672.
    HOGENOMiHOG000231945.
    HOVERGENiHBG052826.
    InParanoidiP25916.
    KOiK11459.
    OMAiRVRPNCK.
    OrthoDBiEOG77T14W.
    PhylomeDBiP25916.
    TreeFamiTF324206.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR018957. Znf_C3HC4_RING-type.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    [Graphical view]
    PfamiPF00097. zf-C3HC4. 1 hit.
    [Graphical view]
    SMARTiSM00184. RING. 1 hit.
    [Graphical view]
    PROSITEiPS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25916-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHRTTRIKIT ELNPHLMCVL CGGYFIDATT IIECLHSFCK TCIVRYLETS    50
    KYCPICDVQV HKTRPLLNIR SDKTLQDIVY KLVPGLFKNE MKRRRDFYAA 100
    HPSADAANGS NEDRGEVADE EKRIITDDEI ISLSIEFFDQ SRLDRKVNKE 150
    KPKEEVNDKR YLRCPAAMTV MHLRKFLRSK MDIPNTFQID VMYEEEPLKD 200
    YYTLMDIAYI YTWRRNGPLP LKYRVRPTCK RMKMSHQRDG LTNAGELESD 250
    SGSDKANSPA GGVPSTSSCL PSPSTPVQSP HPQFPHISST MNGTSNSPSA 300
    NHQSSFASRP RKSSLNGSSA TSSG 324
    Length:324
    Mass (Da):36,708
    Last modified:May 1, 1992 - v1
    Checksum:iAD7DECDB6B29DCE5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64067 Genomic DNA. Translation: AAA37299.1.
    M64068 Genomic DNA. Translation: AAA37301.1. Sequence problems.
    M64279 mRNA. Translation: AAA37300.1.
    BC053708 mRNA. Translation: AAH53708.1.
    BC056384 mRNA. Translation: AAH56384.1.
    CCDSiCCDS15711.1.
    PIRiA39523.
    RefSeqiNP_031578.2. NM_007552.4.
    XP_006497374.1. XM_006497311.1.
    XP_006497375.1. XM_006497312.1.
    UniGeneiMm.289584.

    Genome annotation databases

    EnsembliENSMUST00000028071; ENSMUSP00000028071; ENSMUSG00000026739.
    ENSMUST00000051929; ENSMUSP00000110300; ENSMUSG00000026739.
    GeneIDi12151.
    KEGGimmu:12151.
    UCSCiuc008ily.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64067 Genomic DNA. Translation: AAA37299.1 .
    M64068 Genomic DNA. Translation: AAA37301.1 . Sequence problems.
    M64279 mRNA. Translation: AAA37300.1 .
    BC053708 mRNA. Translation: AAH53708.1 .
    BC056384 mRNA. Translation: AAH56384.1 .
    CCDSi CCDS15711.1.
    PIRi A39523.
    RefSeqi NP_031578.2. NM_007552.4.
    XP_006497374.1. XM_006497311.1.
    XP_006497375.1. XM_006497312.1.
    UniGenei Mm.289584.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2CKL X-ray 2.00 A 1-108 [» ]
    ProteinModelPortali P25916.
    SMRi P25916. Positions 6-103.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198359. 27 interactions.
    DIPi DIP-132N.
    IntActi P25916. 17 interactions.

    PTM databases

    PhosphoSitei P25916.

    Proteomic databases

    PaxDbi P25916.
    PRIDEi P25916.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028071 ; ENSMUSP00000028071 ; ENSMUSG00000026739 .
    ENSMUST00000051929 ; ENSMUSP00000110300 ; ENSMUSG00000026739 .
    GeneIDi 12151.
    KEGGi mmu:12151.
    UCSCi uc008ily.1. mouse.

    Organism-specific databases

    CTDi 648.
    MGIi MGI:88174. Bmi1.

    Phylogenomic databases

    eggNOGi NOG304672.
    HOGENOMi HOG000231945.
    HOVERGENi HBG052826.
    InParanoidi P25916.
    KOi K11459.
    OMAi RVRPNCK.
    OrthoDBi EOG77T14W.
    PhylomeDBi P25916.
    TreeFami TF324206.

    Enzyme and pathway databases

    Reactomei REACT_188970. Oxidative Stress Induced Senescence.

    Miscellaneous databases

    EvolutionaryTracei P25916.
    NextBioi 280483.
    PROi P25916.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P25916.
    Bgeei P25916.
    CleanExi MM_BMI1.
    Genevestigatori P25916.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR018957. Znf_C3HC4_RING-type.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR017907. Znf_RING_CS.
    [Graphical view ]
    Pfami PF00097. zf-C3HC4. 1 hit.
    [Graphical view ]
    SMARTi SM00184. RING. 1 hit.
    [Graphical view ]
    PROSITEi PS00518. ZF_RING_1. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Novel zinc finger gene implicated as myc collaborator by retrovirally accelerated lymphomagenesis in E mu-myc transgenic mice."
      Haupt Y., Alexander W.S., Barri G., Klinken S.P., Adams J.M.
      Cell 65:753-763(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Identification of cooperating oncogenes in E mu-myc transgenic mice by provirus tagging."
      van Lohuiizen M., Verbbeek S., Scheijen B., Wientjens E., Gulden H., Berns A.
      Cell 65:737-752(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain and Hematopoietic.
    4. "Role of Bmi-1 and Ring1A in H2A ubiquitylation and Hox gene silencing."
      Cao R., Tsukada Y., Zhang Y.
      Mol. Cell 20:845-854(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: RECONSTITUTION OF A PRC1-LIKE COMPLEX, INTERACTION WITH RING1; RNF2; CBX8 AND PHC2.
    5. "Structure and E3-ligase activity of the Ring-Ring complex of polycomb proteins Bmi1 and Ring1b."
      Buchwald G., van der Stoop P., Weichenrieder O., Perrakis A., van Lohuizen M., Sixma T.K.
      EMBO J. 25:2465-2474(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-108 IN COMPLEX WITH RNF2 AND ZINC IONS, FUNCTION, INTERACTION WITH RING1, MONOUBIQUITINATION, POLYUBIQUITINATION IN THE PRESENCE OF UBE2D3 AND RING2, SUBUNIT.

    Entry informationi

    Entry nameiBMI1_MOUSE
    AccessioniPrimary (citable) accession number: P25916
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 139 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3