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P25916

- BMI1_MOUSE

UniProt

P25916 - BMI1_MOUSE

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Protein

Polycomb complex protein BMI-1

Gene

Bmi1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2 (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri18 – 5740RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: MGI
  2. sequence-specific DNA binding Source: MGI
  3. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. brain development Source: MGI
  2. cellular process Source: MGI
  3. DNA methylation Source: MGI
  4. embryonic skeletal system development Source: MGI
  5. embryonic skeletal system morphogenesis Source: MGI
  6. histone acetylation Source: MGI
  7. histone ubiquitination Source: MGI
  8. humoral immune response Source: MGI
  9. in utero embryonic development Source: MGI
  10. negative regulation of apoptotic signaling pathway Source: MGI
  11. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  12. positive regulation of B cell proliferation Source: HGNC
  13. positive regulation of immature T cell proliferation in thymus Source: HGNC
  14. positive regulation of ubiquitin-protein transferase activity Source: UniProtKB
  15. rostrocaudal neural tube patterning Source: MGI
  16. skeletal system development Source: MGI
  17. somatic stem cell division Source: MGI
  18. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_188970. Oxidative Stress Induced Senescence.

Names & Taxonomyi

Protein namesi
Recommended name:
Polycomb complex protein BMI-1
Alternative name(s):
Polycomb group RING finger protein 4
Gene namesi
Name:Bmi1
Synonyms:Bmi-1, Pcgf4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:88174. Bmi1.

Subcellular locationi

Nucleus. Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. heterochromatin Source: MGI
  3. nuclear body Source: MGI
  4. nucleus Source: MGI
  5. PcG protein complex Source: MGI
  6. ubiquitin ligase complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 324324Polycomb complex protein BMI-1PRO_0000055988Add
BLAST

Post-translational modificationi

May be polyubiquitinated; which does not lead to proteasomal degradation (By similarity). Monoubiquitinated.By similarity1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiP25916.
PaxDbiP25916.
PRIDEiP25916.

PTM databases

PhosphoSiteiP25916.

Expressioni

Tissue specificityi

Detected in most organs with high expression levels in thymus, heart, brain and testis.

Gene expression databases

BgeeiP25916.
CleanExiMM_BMI1.
ExpressionAtlasiP25916. baseline and differential.
GenevestigatoriP25916.

Interactioni

Subunit structurei

Component of a PRC1-like complex. Interacts with RING1 and RING2. Interacts vwith CBX7 and CBX8. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Cbx8Q9QXV13EBI-927401,EBI-1216641
PHC2Q8IXK02EBI-927401,EBI-713786From a different organism.
Phc2Q9QWH13EBI-927401,EBI-642357
Ring1O357304EBI-927401,EBI-929310
Rnf2Q9CQJ411EBI-927401,EBI-927321

Protein-protein interaction databases

BioGridi198359. 27 interactions.
DIPiDIP-132N.
IntActiP25916. 17 interactions.

Structurei

Secondary structure

1
324
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 124
Helixi13 – 153
Turni19 – 213
Beta strandi22 – 243
Beta strandi26 – 316
Turni32 – 343
Beta strandi37 – 393
Helixi40 – 478
Turni54 – 563
Helixi65 – 684
Beta strandi69 – 713
Helixi73 – 8210
Helixi86 – 10015

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CKLX-ray2.00A1-108[»]
ProteinModelPortaliP25916.
SMRiP25916. Positions 6-103.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25916.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni162 – 22665Interaction with E4F1By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi81 – 9515Nuclear localization signalSequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi249 – 32476Pro/Ser-richAdd
BLAST

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri18 – 5740RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG304672.
HOGENOMiHOG000231945.
HOVERGENiHBG052826.
InParanoidiP25916.
KOiK11459.
OMAiRVRPNCK.
OrthoDBiEOG77T14W.
PhylomeDBiP25916.
TreeFamiTF324206.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00097. zf-C3HC4. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25916 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHRTTRIKIT ELNPHLMCVL CGGYFIDATT IIECLHSFCK TCIVRYLETS
60 70 80 90 100
KYCPICDVQV HKTRPLLNIR SDKTLQDIVY KLVPGLFKNE MKRRRDFYAA
110 120 130 140 150
HPSADAANGS NEDRGEVADE EKRIITDDEI ISLSIEFFDQ SRLDRKVNKE
160 170 180 190 200
KPKEEVNDKR YLRCPAAMTV MHLRKFLRSK MDIPNTFQID VMYEEEPLKD
210 220 230 240 250
YYTLMDIAYI YTWRRNGPLP LKYRVRPTCK RMKMSHQRDG LTNAGELESD
260 270 280 290 300
SGSDKANSPA GGVPSTSSCL PSPSTPVQSP HPQFPHISST MNGTSNSPSA
310 320
NHQSSFASRP RKSSLNGSSA TSSG
Length:324
Mass (Da):36,708
Last modified:May 1, 1992 - v1
Checksum:iAD7DECDB6B29DCE5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64067 Genomic DNA. Translation: AAA37299.1.
M64068 Genomic DNA. Translation: AAA37301.1. Sequence problems.
M64279 mRNA. Translation: AAA37300.1.
BC053708 mRNA. Translation: AAH53708.1.
BC056384 mRNA. Translation: AAH56384.1.
CCDSiCCDS15711.1.
PIRiA39523.
RefSeqiNP_031578.2. NM_007552.4.
XP_006497374.1. XM_006497311.1.
XP_006497375.1. XM_006497312.1.
UniGeneiMm.289584.

Genome annotation databases

EnsembliENSMUST00000028071; ENSMUSP00000028071; ENSMUSG00000026739.
ENSMUST00000051929; ENSMUSP00000110300; ENSMUSG00000026739.
GeneIDi12151.
KEGGimmu:12151.
UCSCiuc008ily.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64067 Genomic DNA. Translation: AAA37299.1 .
M64068 Genomic DNA. Translation: AAA37301.1 . Sequence problems.
M64279 mRNA. Translation: AAA37300.1 .
BC053708 mRNA. Translation: AAH53708.1 .
BC056384 mRNA. Translation: AAH56384.1 .
CCDSi CCDS15711.1.
PIRi A39523.
RefSeqi NP_031578.2. NM_007552.4.
XP_006497374.1. XM_006497311.1.
XP_006497375.1. XM_006497312.1.
UniGenei Mm.289584.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2CKL X-ray 2.00 A 1-108 [» ]
ProteinModelPortali P25916.
SMRi P25916. Positions 6-103.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198359. 27 interactions.
DIPi DIP-132N.
IntActi P25916. 17 interactions.

PTM databases

PhosphoSitei P25916.

Proteomic databases

MaxQBi P25916.
PaxDbi P25916.
PRIDEi P25916.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000028071 ; ENSMUSP00000028071 ; ENSMUSG00000026739 .
ENSMUST00000051929 ; ENSMUSP00000110300 ; ENSMUSG00000026739 .
GeneIDi 12151.
KEGGi mmu:12151.
UCSCi uc008ily.1. mouse.

Organism-specific databases

CTDi 648.
MGIi MGI:88174. Bmi1.

Phylogenomic databases

eggNOGi NOG304672.
HOGENOMi HOG000231945.
HOVERGENi HBG052826.
InParanoidi P25916.
KOi K11459.
OMAi RVRPNCK.
OrthoDBi EOG77T14W.
PhylomeDBi P25916.
TreeFami TF324206.

Enzyme and pathway databases

Reactomei REACT_188970. Oxidative Stress Induced Senescence.

Miscellaneous databases

EvolutionaryTracei P25916.
NextBioi 280483.
PROi P25916.
SOURCEi Search...

Gene expression databases

Bgeei P25916.
CleanExi MM_BMI1.
ExpressionAtlasi P25916. baseline and differential.
Genevestigatori P25916.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view ]
Pfami PF00097. zf-C3HC4. 1 hit.
[Graphical view ]
SMARTi SM00184. RING. 1 hit.
[Graphical view ]
PROSITEi PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Novel zinc finger gene implicated as myc collaborator by retrovirally accelerated lymphomagenesis in E mu-myc transgenic mice."
    Haupt Y., Alexander W.S., Barri G., Klinken S.P., Adams J.M.
    Cell 65:753-763(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Identification of cooperating oncogenes in E mu-myc transgenic mice by provirus tagging."
    van Lohuiizen M., Verbbeek S., Scheijen B., Wientjens E., Gulden H., Berns A.
    Cell 65:737-752(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain and Hematopoietic.
  4. "Role of Bmi-1 and Ring1A in H2A ubiquitylation and Hox gene silencing."
    Cao R., Tsukada Y., Zhang Y.
    Mol. Cell 20:845-854(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: RECONSTITUTION OF A PRC1-LIKE COMPLEX, INTERACTION WITH RING1; RNF2; CBX8 AND PHC2.
  5. "Structure and E3-ligase activity of the Ring-Ring complex of polycomb proteins Bmi1 and Ring1b."
    Buchwald G., van der Stoop P., Weichenrieder O., Perrakis A., van Lohuizen M., Sixma T.K.
    EMBO J. 25:2465-2474(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-108 IN COMPLEX WITH RNF2 AND ZINC IONS, FUNCTION, INTERACTION WITH RING1, MONOUBIQUITINATION, POLYUBIQUITINATION IN THE PRESENCE OF UBE2D3 AND RING2, SUBUNIT.

Entry informationi

Entry nameiBMI1_MOUSE
AccessioniPrimary (citable) accession number: P25916
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: October 29, 2014
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3