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Protein

Polycomb complex protein BMI-1

Gene

Bmi1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A. In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2 (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri18 – 57RING-typePROSITE-ProRule annotationAdd BLAST40

GO - Molecular functioni

  • chromatin binding Source: MGI
  • promoter-specific chromatin binding Source: MGI
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: MGI
  • sequence-specific DNA binding Source: MGI
  • ubiquitin-protein transferase activator activity Source: MGI
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • brain development Source: MGI
  • cellular process Source: MGI
  • cellular response to dexamethasone stimulus Source: Ensembl
  • cellular response to interleukin-1 Source: Ensembl
  • DNA methylation Source: MGI
  • embryonic skeletal system development Source: MGI
  • embryonic skeletal system morphogenesis Source: MGI
  • hematopoietic stem cell homeostasis Source: MGI
  • histone acetylation Source: MGI
  • histone H2A-K119 monoubiquitination Source: UniProtKB
  • histone ubiquitination Source: MGI
  • humoral immune response Source: MGI
  • in utero embryonic development Source: MGI
  • negative regulation of apoptotic signaling pathway Source: MGI
  • negative regulation of gene expression, epigenetic Source: UniProtKB
  • negative regulation of transcription by RNA polymerase II Source: MGI
  • positive regulation of B cell proliferation Source: HGNC
  • positive regulation of immature T cell proliferation in thymus Source: HGNC
  • positive regulation of ubiquitin-protein transferase activity Source: UniProtKB
  • regulation of adaxial/abaxial pattern formation Source: MGI
  • rostrocaudal neural tube patterning Source: MGI
  • skeletal system development Source: MGI
  • somatic stem cell division Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionChromatin regulator, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-2559580 Oxidative Stress Induced Senescence
R-MMU-3108214 SUMOylation of DNA damage response and repair proteins
R-MMU-4551638 SUMOylation of chromatin organization proteins
R-MMU-8939243 RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known
R-MMU-8943724 Regulation of PTEN gene transcription
R-MMU-8953750 Transcriptional Regulation by E2F6

Names & Taxonomyi

Protein namesi
Recommended name:
Polycomb complex protein BMI-1
Alternative name(s):
Polycomb group RING finger protein 4
Gene namesi
Name:Bmi1
Synonyms:Bmi-1, Pcgf4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:88174 Bmi1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000559881 – 324Polycomb complex protein BMI-1Add BLAST324

Post-translational modificationi

May be polyubiquitinated; which does not lead to proteasomal degradation (By similarity). Monoubiquitinated.By similarity1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

EPDiP25916
PaxDbiP25916
PeptideAtlasiP25916
PRIDEiP25916

PTM databases

iPTMnetiP25916
PhosphoSitePlusiP25916

Expressioni

Tissue specificityi

Detected in most organs with high expression levels in thymus, heart, brain and testis.

Gene expression databases

BgeeiENSMUSG00000026739
CleanExiMM_BMI1
ExpressionAtlasiP25916 baseline and differential
GenevisibleiP25916 MM

Interactioni

Subunit structurei

Component of a PRC1-like complex (PubMed:16359901). Identified in a PRC1-like HPRC-H complex with CBX2, CBX4, CBX8, PHC1, PHC2, PHC3, RING1 and RNF2 (By similarity). Interacts with RNF2/RING2 (PubMed:16359901, PubMed:16710298). Interacts with RING1 (PubMed:16359901, PubMed:16710298). Part of a complex that contains RNF2, UB2D3 and BMI1, where RNF2 and BMI1 form a tight heterodimer, and UB2D3 interacts only with RNF2. The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A. Interacts with CBX7 and CBX8 (PubMed:16359901). Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1 (By similarity). Interacts with PHC2 (PubMed:16359901).By similarity2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi198359, 26 interactors
CORUMiP25916
DIPiDIP-132N
IntActiP25916, 21 interactors
MINTiP25916
STRINGi10090.ENSMUSP00000028071

Structurei

Secondary structure

1324
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 12Combined sources4
Helixi13 – 15Combined sources3
Turni19 – 21Combined sources3
Beta strandi22 – 24Combined sources3
Beta strandi26 – 31Combined sources6
Turni32 – 34Combined sources3
Beta strandi37 – 39Combined sources3
Helixi40 – 47Combined sources8
Turni54 – 56Combined sources3
Helixi65 – 68Combined sources4
Beta strandi69 – 71Combined sources3
Helixi73 – 82Combined sources10
Helixi86 – 100Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CKLX-ray2.00A1-108[»]
ProteinModelPortaliP25916
SMRiP25916
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25916

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni160 – 180Interaction with PHC2By similarityAdd BLAST21
Regioni162 – 226Interaction with E4F1By similarityAdd BLAST65

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi81 – 95Nuclear localization signalSequence analysisAdd BLAST15

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi249 – 324Pro/Ser-richAdd BLAST76

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri18 – 57RING-typePROSITE-ProRule annotationAdd BLAST40

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2660 Eukaryota
ENOG410XPCN LUCA
GeneTreeiENSGT00550000074463
HOGENOMiHOG000231945
HOVERGENiHBG052826
InParanoidiP25916
KOiK11459
OMAiDIPNTYQ
OrthoDBiEOG091G09IB
PhylomeDBiP25916
TreeFamiTF324206

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR032443 RAWUL
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF16207 RAWUL, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

P25916-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHRTTRIKIT ELNPHLMCVL CGGYFIDATT IIECLHSFCK TCIVRYLETS
60 70 80 90 100
KYCPICDVQV HKTRPLLNIR SDKTLQDIVY KLVPGLFKNE MKRRRDFYAA
110 120 130 140 150
HPSADAANGS NEDRGEVADE EKRIITDDEI ISLSIEFFDQ SRLDRKVNKE
160 170 180 190 200
KPKEEVNDKR YLRCPAAMTV MHLRKFLRSK MDIPNTFQID VMYEEEPLKD
210 220 230 240 250
YYTLMDIAYI YTWRRNGPLP LKYRVRPTCK RMKMSHQRDG LTNAGELESD
260 270 280 290 300
SGSDKANSPA GGVPSTSSCL PSPSTPVQSP HPQFPHISST MNGTSNSPSA
310 320
NHQSSFASRP RKSSLNGSSA TSSG
Length:324
Mass (Da):36,708
Last modified:May 1, 1992 - v1
Checksum:iAD7DECDB6B29DCE5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64067 Genomic DNA Translation: AAA37299.1
M64068 Genomic DNA Translation: AAA37301.1 Sequence problems.
M64279 mRNA Translation: AAA37300.1
BC053708 mRNA Translation: AAH53708.1
BC056384 mRNA Translation: AAH56384.1
CCDSiCCDS15711.1
PIRiA39523
RefSeqiNP_031578.2, NM_007552.4
XP_006497374.1, XM_006497311.2
XP_006497375.1, XM_006497312.3
UniGeneiMm.289584

Genome annotation databases

EnsembliENSMUST00000028071; ENSMUSP00000028071; ENSMUSG00000026739
ENSMUST00000051929; ENSMUSP00000110300; ENSMUSG00000026739
GeneIDi12151
KEGGimmu:12151
UCSCiuc008ily.1 mouse

Similar proteinsi

Entry informationi

Entry nameiBMI1_MOUSE
AccessioniPrimary (citable) accession number: P25916
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: May 23, 2018
This is version 171 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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