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Protein

Polycomb complex protein BMI-1

Gene

Bmi1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2 (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri18 – 5740RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • chromatin binding Source: MGI
  • sequence-specific DNA binding Source: MGI
  • ubiquitin-protein transferase activator activity Source: MGI
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • brain development Source: MGI
  • cellular process Source: MGI
  • DNA methylation Source: MGI
  • embryonic skeletal system development Source: MGI
  • embryonic skeletal system morphogenesis Source: MGI
  • hematopoietic stem cell homeostasis Source: MGI
  • histone acetylation Source: MGI
  • histone ubiquitination Source: MGI
  • humoral immune response Source: MGI
  • in utero embryonic development Source: MGI
  • negative regulation of apoptotic signaling pathway Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of B cell proliferation Source: HGNC
  • positive regulation of immature T cell proliferation in thymus Source: HGNC
  • positive regulation of ubiquitin-protein transferase activity Source: UniProtKB
  • rostrocaudal neural tube patterning Source: MGI
  • skeletal system development Source: MGI
  • somatic stem cell division Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_305463. Oxidative Stress Induced Senescence.
REACT_360954. SUMOylation of DNA damage response and repair proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Polycomb complex protein BMI-1
Alternative name(s):
Polycomb group RING finger protein 4
Gene namesi
Name:Bmi1
Synonyms:Bmi-1, Pcgf4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:88174. Bmi1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • heterochromatin Source: MGI
  • nuclear body Source: MGI
  • nucleus Source: MGI
  • PcG protein complex Source: MGI
  • ubiquitin ligase complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 324324Polycomb complex protein BMI-1PRO_0000055988Add
BLAST

Post-translational modificationi

May be polyubiquitinated; which does not lead to proteasomal degradation (By similarity). Monoubiquitinated.By similarity1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiP25916.
PaxDbiP25916.
PRIDEiP25916.

PTM databases

PhosphoSiteiP25916.

Expressioni

Tissue specificityi

Detected in most organs with high expression levels in thymus, heart, brain and testis.

Gene expression databases

BgeeiP25916.
CleanExiMM_BMI1.
ExpressionAtlasiP25916. baseline and differential.
GenevisibleiP25916. MM.

Interactioni

Subunit structurei

Component of a PRC1-like complex. Interacts with RING1 and RING2. Interacts vwith CBX7 and CBX8. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Cbx8Q9QXV13EBI-927401,EBI-1216641
PHC2Q8IXK02EBI-927401,EBI-713786From a different organism.
Phc2Q9QWH13EBI-927401,EBI-642357
Ring1O357304EBI-927401,EBI-929310
Rnf2Q9CQJ411EBI-927401,EBI-927321

Protein-protein interaction databases

BioGridi198359. 27 interactions.
DIPiDIP-132N.
IntActiP25916. 17 interactions.
STRINGi10090.ENSMUSP00000028071.

Structurei

Secondary structure

1
324
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 124Combined sources
Helixi13 – 153Combined sources
Turni19 – 213Combined sources
Beta strandi22 – 243Combined sources
Beta strandi26 – 316Combined sources
Turni32 – 343Combined sources
Beta strandi37 – 393Combined sources
Helixi40 – 478Combined sources
Turni54 – 563Combined sources
Helixi65 – 684Combined sources
Beta strandi69 – 713Combined sources
Helixi73 – 8210Combined sources
Helixi86 – 10015Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CKLX-ray2.00A1-108[»]
ProteinModelPortaliP25916.
SMRiP25916. Positions 6-103.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25916.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni162 – 22665Interaction with E4F1By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi81 – 9515Nuclear localization signalSequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi249 – 32476Pro/Ser-richAdd
BLAST

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri18 – 5740RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG304672.
HOGENOMiHOG000231945.
HOVERGENiHBG052826.
InParanoidiP25916.
KOiK11459.
OMAiNKRYLQC.
OrthoDBiEOG77T14W.
PhylomeDBiP25916.
TreeFamiTF324206.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00097. zf-C3HC4. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25916-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHRTTRIKIT ELNPHLMCVL CGGYFIDATT IIECLHSFCK TCIVRYLETS
60 70 80 90 100
KYCPICDVQV HKTRPLLNIR SDKTLQDIVY KLVPGLFKNE MKRRRDFYAA
110 120 130 140 150
HPSADAANGS NEDRGEVADE EKRIITDDEI ISLSIEFFDQ SRLDRKVNKE
160 170 180 190 200
KPKEEVNDKR YLRCPAAMTV MHLRKFLRSK MDIPNTFQID VMYEEEPLKD
210 220 230 240 250
YYTLMDIAYI YTWRRNGPLP LKYRVRPTCK RMKMSHQRDG LTNAGELESD
260 270 280 290 300
SGSDKANSPA GGVPSTSSCL PSPSTPVQSP HPQFPHISST MNGTSNSPSA
310 320
NHQSSFASRP RKSSLNGSSA TSSG
Length:324
Mass (Da):36,708
Last modified:May 1, 1992 - v1
Checksum:iAD7DECDB6B29DCE5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64067 Genomic DNA. Translation: AAA37299.1.
M64068 Genomic DNA. Translation: AAA37301.1. Sequence problems.
M64279 mRNA. Translation: AAA37300.1.
BC053708 mRNA. Translation: AAH53708.1.
BC056384 mRNA. Translation: AAH56384.1.
CCDSiCCDS15711.1.
PIRiA39523.
RefSeqiNP_031578.2. NM_007552.4.
XP_006497374.1. XM_006497311.2.
XP_006497375.1. XM_006497312.2.
UniGeneiMm.289584.

Genome annotation databases

EnsembliENSMUST00000028071; ENSMUSP00000028071; ENSMUSG00000026739.
ENSMUST00000051929; ENSMUSP00000110300; ENSMUSG00000026739.
GeneIDi12151.
KEGGimmu:12151.
UCSCiuc008ily.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64067 Genomic DNA. Translation: AAA37299.1.
M64068 Genomic DNA. Translation: AAA37301.1. Sequence problems.
M64279 mRNA. Translation: AAA37300.1.
BC053708 mRNA. Translation: AAH53708.1.
BC056384 mRNA. Translation: AAH56384.1.
CCDSiCCDS15711.1.
PIRiA39523.
RefSeqiNP_031578.2. NM_007552.4.
XP_006497374.1. XM_006497311.2.
XP_006497375.1. XM_006497312.2.
UniGeneiMm.289584.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CKLX-ray2.00A1-108[»]
ProteinModelPortaliP25916.
SMRiP25916. Positions 6-103.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198359. 27 interactions.
DIPiDIP-132N.
IntActiP25916. 17 interactions.
STRINGi10090.ENSMUSP00000028071.

PTM databases

PhosphoSiteiP25916.

Proteomic databases

MaxQBiP25916.
PaxDbiP25916.
PRIDEiP25916.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028071; ENSMUSP00000028071; ENSMUSG00000026739.
ENSMUST00000051929; ENSMUSP00000110300; ENSMUSG00000026739.
GeneIDi12151.
KEGGimmu:12151.
UCSCiuc008ily.1. mouse.

Organism-specific databases

CTDi648.
MGIiMGI:88174. Bmi1.

Phylogenomic databases

eggNOGiNOG304672.
HOGENOMiHOG000231945.
HOVERGENiHBG052826.
InParanoidiP25916.
KOiK11459.
OMAiNKRYLQC.
OrthoDBiEOG77T14W.
PhylomeDBiP25916.
TreeFamiTF324206.

Enzyme and pathway databases

ReactomeiREACT_305463. Oxidative Stress Induced Senescence.
REACT_360954. SUMOylation of DNA damage response and repair proteins.

Miscellaneous databases

EvolutionaryTraceiP25916.
NextBioi280483.
PROiP25916.
SOURCEiSearch...

Gene expression databases

BgeeiP25916.
CleanExiMM_BMI1.
ExpressionAtlasiP25916. baseline and differential.
GenevisibleiP25916. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00097. zf-C3HC4. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Novel zinc finger gene implicated as myc collaborator by retrovirally accelerated lymphomagenesis in E mu-myc transgenic mice."
    Haupt Y., Alexander W.S., Barri G., Klinken S.P., Adams J.M.
    Cell 65:753-763(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Identification of cooperating oncogenes in E mu-myc transgenic mice by provirus tagging."
    van Lohuiizen M., Verbbeek S., Scheijen B., Wientjens E., Gulden H., Berns A.
    Cell 65:737-752(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain and Hematopoietic.
  4. "Role of Bmi-1 and Ring1A in H2A ubiquitylation and Hox gene silencing."
    Cao R., Tsukada Y., Zhang Y.
    Mol. Cell 20:845-854(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: RECONSTITUTION OF A PRC1-LIKE COMPLEX, INTERACTION WITH RING1; RNF2; CBX8 AND PHC2.
  5. "Structure and E3-ligase activity of the Ring-Ring complex of polycomb proteins Bmi1 and Ring1b."
    Buchwald G., van der Stoop P., Weichenrieder O., Perrakis A., van Lohuizen M., Sixma T.K.
    EMBO J. 25:2465-2474(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-108 IN COMPLEX WITH RNF2 AND ZINC IONS, FUNCTION, INTERACTION WITH RING1, MONOUBIQUITINATION, POLYUBIQUITINATION IN THE PRESENCE OF UBE2D3 AND RING2, SUBUNIT.

Entry informationi

Entry nameiBMI1_MOUSE
AccessioniPrimary (citable) accession number: P25916
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: June 24, 2015
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.