Reviewed, UniProtKB/Swiss-Prot P25910 (BLAB_BACFR)

Names and origin

Protein names

Recommended name:
    Beta-lactamase type II
    EC=3.5.2.6
Alternative name(s):
    Penicillinase
    Cephalosporinase
    Imipenem-cefoxitin hydrolyzing enzyme

Gene names

Name:	ccrA
Synonyms:	cfiA

Organism

Bacteroides fragilis

Taxonomic identifier

817 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Can hydrolyze carbapenem compounds.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Cofactor	Binds 2 zinc ions per subunit.
Subunit structure	Monomer.
Sequence similarities	Belongs to the class-B beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 17

17

Chain

18 – 249

232

Beta-lactamase type II

PRO_0000016945

Sites

Metal binding

99

1

Zinc 1 Ref.3

Metal binding

101

1

Zinc 1 Ref.3

Metal binding

103

1

Zinc 2

Metal binding

162

1

Zinc 1 Ref.3

Metal binding

181

1

Zinc 2

Metal binding

223

1

Zinc 2

Secondary structure

1

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249

Helix Strand Turn

Details...

Beta strand

25 – 32

8

Beta strand

34 – 46

13

Turn

47 – 49

3

Beta strand

50 – 61

12

Beta strand

64 – 69

6

Helix

74 – 88

15

Beta strand

91 – 96

6

Beta strand

98 – 101

4

Helix

102 – 105

4

Helix

108 – 113

6

Beta strand

117 – 121

5

Helix

122 – 131

10

Beta strand

137 – 148

12

Beta strand

151 – 156

6

Beta strand

161 – 163

3

Beta strand

168 – 170

3

Turn

172 – 174

3

Beta strand

176 – 180

5

Turn

199 – 201

3

Helix

202 – 212

11

Beta strand

217 – 224

8

Helix

230 – 246

17

Sequences

	Sequence		Length	Mass (Da)
	P25910-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 972B5A594DF5D40D	FASTA	249	27,257
	10 20 30 40 50 60 MKTVFILISM LFPVAVMAQK SVKISDDISI TQLSDKVYTY VSLAEIEGWG MVPSNGMIVI 70 80 90 100 110 120 NNHQAALLDT PINDAQTEML VNWVTDSLHA KVTTFIPNHW HGDCIGGLGY LQRKGVQSYA 130 140 150 160 170 180 NQMTIDLAKE KGLPVPEHGF TDSLTVSLDG MPLQCYYLGG GHATDNIVVW LPTENILFGG 190 200 210 220 230 240 CMLKDNQATS IGNISDADVT AWPKTLDKVK AKFPSARYVV PGHGDYGGTE LIEHTKQIVN QYIESTSKP

References

[1]	"Cloning and sequencing of the class B beta-lactamase gene (ccrA) from Bacteroides fragilis TAL3636." Rasmussen B.A., Gluzman Y., Tally F.P. Antimicrob. Agents Chemother. 34:1590-1592(1990) [PubMed: 2121094] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: TAL3636.
[2]	"Escherichia coli chromosomal mutations that permit direct cloning of the Bacteroides fragilis metallo-beta-lactamase gene, ccrA." Rasmussen B.A., Gluzman Y., Tally F.P. Mol. Microbiol. 5:1211-1219(1991) [PubMed: 1956298] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: TAL3636.
[3]	"Sequencing the gene for an imipenem-cefoxitin-hydrolyzing enzyme (CfiA) from Bacteroides fragilis TAL2480 reveals strong similarity between CfiA and Bacillus cereus beta-lactamase II." Thompson J.S., Malamy M.H. J. Bacteriol. 172:2584-2593(1990) [PubMed: 2110145] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: TAL2480.
[4]	"Crystal structure of the wide-spectrum binuclear zinc beta-lactamase from Bacteroides fragilis." Concha N.O., Rasmusssen B.A., Bush K., Herzberg O. Structure 4:823-836(1996) [PubMed: 8805566] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS).
[5]	"Crystal structures of the cadmium- and mercury-substituted metallo-beta-lactamase from Bacteroides fragilis." Concha N.O., Rasmussen B.A., Bush K., Herzberg O. Protein Sci. 6:2671-2676(1997) [PubMed: 9416622] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS).
[6]	"X-ray structure of the ZnII beta-Lactamase from Bacteroides fragilis in an orthorhombic crystal form." Carfi A., Duee E., Soto R.-P., Galleni M., Frere J.-M., Dideberg O. Acta Crystallogr. D 54:47-57(1998) [PubMed: 9761816] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS). Strain: TAL2480.
[7]	"Unanticipated inhibition of the metallo-beta-lactamase from Bacteroides fragilis by 4-morpholineethanesulfonic acid (MES): a crystallographic study at 1.85-A resolution." Fitzgerald P.M., Wu J.K., Toney J.H. Biochemistry 37:6791-6800(1998) [PubMed: 9578564] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS).
[8]	"Structural consequences of the active site substitution Cys181 --> Ser in metallo-beta-lactamase from Bacteroides fragilis." Li Z., Rasmussen B.A., Herzberg O. Protein Sci. 8:249-252(1999) [PubMed: 10210203] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M63556 Genomic DNA. Translation: AAA22904.1.
M34831 Genomic DNA. Translation: AAA22907.1.

PIR

A35263. A44999.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1A7T	X-ray	1.85	A/B	18-249	[»]
1A8T	X-ray	2.55	A/B	18-249	[»]
1HLK	X-ray	2.50	A/B	21-247	[»]
1KR3	X-ray	2.50	A/B	18-249	[»]
1ZNB	X-ray	1.85	A/B	18-249	[»]
2BMI	X-ray	2.00	A/B	18-249	[»]
2ZNB	X-ray	2.15	A/B	18-249	[»]
3ZNB	X-ray	2.70	A/B	18-249	[»]
4ZNB	X-ray	2.65	A/B	18-249	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

3.5.2.6. 868.

Family and domain databases

InterPro

IPR001018. Beta-lactamase_class-B_CS.
IPR001279. Blactmase-like.
[Graphical view]

Pfam

PF00753. Lactamase_B. 1 hit.
[Graphical view]

PROSITE

PS00743. BETA_LACTAMASE_B_1. 1 hit.
PS00744. BETA_LACTAMASE_B_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

BLAB_BACFR

Accession

Primary (citable) accession number: P25910

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	June 16, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)