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Protein

Pyridoxine 4-dehydrogenase

Gene

pdxI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NAD(P)H-dependent reduction of pyridoxal to pyridoxine in vitro. Is not able to reduce 4-pyridoxate, and to oxidize pyridoxine or pyridoxamine (PubMed:27941785). Has Kemp eliminase activity towards the non-physiological substrate 5-nitrobenzisoxazole, producing 4-nitro-2-cyanophenol; this activity is not considered to be physiologically relevant (PubMed:21332126).2 Publications

Catalytic activityi

Pyridoxine + NADP+ = pyridoxal + NADPH.1 Publication
Pyridoxine + NAD+ = pyridoxal + NADH.1 Publication

Kineticsi

kcat is 0.11 sec(-1) for the Kemp eliminase activity towards 5-nitrobenzisoxazole.1 Publication
  1. KM=270 µM for 5-nitrobenzisoxazole1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei59Proton donorBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi210 – 218NADPBy similarity9

    GO - Molecular functioni

    Keywordsi

    Molecular functionOxidoreductase
    LigandNAD, NADP

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11309-MONOMER.
    MetaCyc:EG11309-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyridoxine 4-dehydrogenase1 Publication (EC:1.1.1.651 Publication)
    Gene namesi
    Name:pdxI1 Publication
    Synonyms:ydbCImported
    Ordered Locus Names:b1406, JW1403
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11309. ydbC.

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: EcoCyc

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000703931 – 286Pyridoxine 4-dehydrogenaseAdd BLAST286

    Proteomic databases

    PaxDbiP25906.
    PRIDEiP25906.

    2D gel databases

    SWISS-2DPAGEiP25906.

    Interactioni

    Protein-protein interaction databases

    BioGridi4263019. 9 interactors.
    DIPiDIP-11632N.
    IntActiP25906. 4 interactors.
    STRINGi316385.ECDH10B_1532.

    Structurei

    3D structure databases

    ProteinModelPortaliP25906.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4107S5I. Bacteria.
    ENOG410XRV3. LUCA.
    HOGENOMiHOG000250284.
    InParanoidiP25906.
    PhylomeDBiP25906.

    Family and domain databases

    CDDicd06660. Aldo_ket_red. 1 hit.
    Gene3Di3.20.20.100. 1 hit.
    InterProiView protein in InterPro
    IPR023210. NADP_OxRdtase_dom.
    PfamiView protein in Pfam
    PF00248. Aldo_ket_red. 1 hit.
    SUPFAMiSSF51430. SSF51430. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P25906-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSSNTFTLGT KSVNRLGYGA MQLAGPGVFG PPRDRHVAIT VLREALALGV
    60 70 80 90 100
    NHIDTSDFYG PHVTNQIIRE ALYPYSDDLT IVTKIGARRG EDASWLPAFS
    110 120 130 140 150
    PAELQKAVHD NLRNLGLDVL DVVNLRVMMG DGHGPAEGSI EASLTVLAEM
    160 170 180 190 200
    QQQGLVKHIG LSNVTPTQVA EARKIAEIVC VQNEYNIAHR ADDAMIDALA
    210 220 230 240 250
    HDGIAYVPFF PLGGFTPLQS STLSDVAASL GATPMQVALA WLLQRSPNIL
    260 270 280
    LIPGTSSVAH LRENMAAEKL HLSEEVLSTL DGISRE
    Length:286
    Mass (Da):30,706
    Last modified:May 1, 1992 - v1
    Checksum:i82B587AEA9115EF9
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X62680 Genomic DNA. Translation: CAA44553.1.
    U00096 Genomic DNA. Translation: AAC74488.1.
    AP009048 Genomic DNA. Translation: BAA15021.1.
    X97452 Genomic DNA. Translation: CAA66103.1.
    PIRiA48399.
    RefSeqiNP_415924.1. NC_000913.3.
    WP_000097801.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74488; AAC74488; b1406.
    BAA15021; BAA15021; BAA15021.
    GeneIDi945980.
    KEGGiecj:JW1403.
    eco:b1406.
    PATRICifig|1411691.4.peg.865.

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

    Entry informationi

    Entry nameiPDXI_ECOLI
    AccessioniPrimary (citable) accession number: P25906
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: July 5, 2017
    This is version 124 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families