Reviewed, UniProtKB/Swiss-Prot P25892 (RIP9_MAIZE)

Names and origin

Protein names

Recommended name:
    Ribosome-inactivating protein 9
    EC=3.2.2.22
Alternative name(s):
    rRNA N-glycosidase
    B-32 protein

Gene names

Name:

CRIP9

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Possesses features of some constitutive defense agent. The coordinate Opaque-2-controlled synthesis of this protein and the major seed storage proteins (zeins) may provide the germinating seedling with both nutritional benefits and protection against pathogen invasion of the surrounding endosperm.
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Tissue specificity	Accumulates to high levels in seeds.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Cytoplasm
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	Ribosome-inactivating protein 9		PRO_0000221405
Regions
	Compositional bias	183 – 189	7	Poly-Ala
	Compositional bias	287 – 295	9	Poly-Ala
Sites
	Active site	208	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P25892-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 978789A2DD2BBF3C	FASTA	304	33,514
	10 20 30 40 50 60 MAETNPELSD LMAQTNKKIV PKFTEIFPVE DVNYPYSAFI ASVRKDVIKH CTDHKGIFQP 70 80 90 100 110 120 VLPPEKKVPE LWFYTELKTR TSSITLAIRM DNLYLVGFRT PGGVWWEFGK AGDTHLLGDN 130 140 150 160 170 180 PRWLGFGGRY QDLIGNKGLE TVTMGRAEMT RAVNDLAKKK KMATLEEEEV QMQMQMPEAA 190 200 210 220 230 240 ELAAAAAAAD PQADTKSKLV KLVVMVCEGL RFNTVSRTVD AGFNSQHGVT LTVTQGKQVQ 250 260 270 280 290 300 KWDRISKAAF EWADHPTAVI PDMQKLGIKD KNEAARIVAL VKNQTTAAAA AATAASADND DDEA

References

[1]

"A maize ribosome-inactivating protein is controlled by the transcriptional activator Opaque-2." Bass H.W., Webster C., Obrian G.R., Roberts J.K.M., Boston R.S. Plant Cell 4:225-234(1992) [PubMed: 1633495] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 282-301. Strain: cv. Wisconsin 64A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M83927 mRNA. Translation: AAA33454.1.
3D structure databases
SMR	P25892. Positions 22-285.
ModBase	Search...
Organism-specific databases
Gramene	P25892.
MaizeGDB	30000.
Enzyme and pathway databases
BRENDA	3.2.2.22. 289.
Family and domain databases
InterPro	IPR001574. Ribosome_inactivat_prot. IPR017988. Ribosome_inactivat_prot_CS. IPR016138. Ribosome_inactivat_prot_sub1. IPR017989. Ribosome_inactivat_prot_subgr. [Graphical view]
Gene3D	G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit.
Pfam	PF00161. RIP. 1 hit. [Graphical view]
PRINTS	PR00396. SHIGARICIN.
PROSITE	PS00275. SHIGA_RICIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIP9_MAIZE

Accession

Primary (citable) accession number: P25892

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	January 19, 2010
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)