Reviewed, UniProtKB/Swiss-Prot P25891 (RIP3_MAIZE)

Names and origin

Protein names

Recommended name:
    Ribosome-inactivating protein 3
Alternative name(s):
    rRNA N-glycosidase
    EC=3.2.2.22
    B-32 protein

Gene names

Name:

CRIP3

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Possesses features of some constitutive defense agent. The coordinate Opaque-2-controlled synthesis of this protein and the major seed storage proteins (zeins) may provide the germinating seedling with both nutritional benefits and protection against pathogen invasion of the surrounding endosperm.
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Tissue specificity	Accumulates to high levels in seeds.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Cytoplasm
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
Technical term	3D-structure
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Ribosome-inactivating protein 3		PRO_0000221404
Regions
	Compositional bias	183 – 188	6	Poly-Ala
Sites
	Active site	207	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P25891-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 6877DBE944F7A127	FASTA	300	33,257
	10 20 30 40 50 60 MAEITLEPSD LMAQTNKRIV PKFTEIFPVE DANYPYSAFI ASVRKDVIKH CTDHKGIFQP 70 80 90 100 110 120 VLPPEKKVPE LWLYTELKTR TSSITLAIRM DNLYLVGFRT PGGVWWEFGK DGDTHLLGDN 130 140 150 160 170 180 PRWLGFGGRY QDLIGNKGLE TVTMGRAEMT RAVNDLAKKK KMATLEEEEV QMQMQMPEAA 190 200 210 220 230 240 DLAAAAAADP QADTKSKLVK LVVMVCEGLR FNTVSRTVDA GFNSQHGVTL TVTQGKQVQK 250 260 270 280 290 300 WDRISKAAFE WADHPTAVIP DMQKLGIKDK NEAARIVALV KNQTTACATA ASADNDDDEA

References

[1]	"A maize ribosome-inactivating protein is controlled by the transcriptional activator Opaque-2." Bass H.W., Webster C., Obrian G.R., Roberts J.K.M., Boston R.S. Plant Cell 4:225-234(1992) [PubMed: 1633495] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M83926 mRNA. Translation: AAA33453.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PQG	X-ray	2.38	A/B	22-286	[»]

ModBase

Search...

Organism-specific databases

Gramene

P25891.

MaizeGDB

30000.

Enzyme and pathway databases

BRENDA

3.2.2.22. 289.

Family and domain databases

InterPro

IPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR017989. Ribosome_inactivat_prot_subgr.
[Graphical view]

Gene3D

G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit.

Pfam

PF00161. RIP. 1 hit.
[Graphical view]

PRINTS

PR00396. SHIGARICIN.

PROSITE

PS00275. SHIGA_RICIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RIP3_MAIZE

Accession

Primary (citable) accession number: P25891

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)