P25888 (RHLE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent RNA helicase rhlE
EC=3.6.4.13

Gene names

Name:	rhlE
Ordered Locus Names:	b0797, JW0781

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as an ATP-dependent RNA helicase, able to unwind ds-RNA. Ref.9
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Interacts with pcnB. Ref.7
Induction	Transiently induced by cold shock in a PNPase-dependent fashion. Ref.8
Sequence similarities	Belongs to the DEAD box helicase family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent RNA helicase activity Inferred from direct assay Ref.9. Source: EcoCyc RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	ATP-dependent RNA helicase rhlE		PRO_0000055108
Regions
	Domain	32 – 208	177	Helicase ATP-binding
	Domain	219 – 381	163	Helicase C-terminal
	Nucleotide binding	45 – 52	8	ATP By similarity
	Motif	1 – 29	29	Q motif
	Motif	156 – 159	4	DEAD box
Experimental info
	Sequence conflict	55	1	F → G Ref.6
	Sequence conflict	230	1	K → E Ref.6
	Sequence conflict	258	1	A → G Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P25888 [UniParc]. Last modified June 1, 1994. Version 3. Checksum: 7576B35B7901671F	FASTA	454	49,989
	10 20 30 40 50 60 MSFDSLGLSP DILRAVAEQG YREPTPIQQQ AIPAVLEGRD LMASAQTGTG KTAGFTLPLL 70 80 90 100 110 120 QHLITRQPHA KGRRPVRALI LTPTRELAAQ IGENVRDYSK YLNIRSLVVF GGVSINPQMM 130 140 150 160 170 180 KLRGGVDVLV ATPGRLLDLE HQNAVKLDQV EILVLDEADR MLDMGFIHDI RRVLTKLPAK 190 200 210 220 230 240 RQNLLFSATF SDDIKALAEK LLHNPLEIEV ARRNTASDQV TQHVHFVDKK RKRELLSHMI 250 260 270 280 290 300 GKGNWQQVLV FTRTKHGANH LAEQLNKDGI RSAAIHGNKS QGARTRALAD FKSGDIRVLV 310 320 330 340 350 360 ATDIAARGLD IEELPHVVNY ELPNVPEDYV HRIGRTGRAA ATGEALSLVC VDEHKLLRDI 370 380 390 400 410 420 EKLLKKEIPR IAIPGYEPDP SIKAEPIQNG RQQRGGGGRG QGGGRGQQQP RRGEGGAKSA 430 440 450 SAKPAEKPSR RLGDAKPAGE QQRRRRPRKP AAAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of the rhlE gene of Escherichia coli." Ohmori H. Jpn. J. Genet. 69:1-12(1994) [PubMed: 8037924] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	Erratum Ohmori H. Jpn. J. Genet. 69:425-425(1994) Cited for: SEQUENCE REVISION TO C-TERMINUS.
[3]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"rhlB, a new Escherichia coli K-12 gene with an RNA helicase-like protein sequence motif, one of at least five such possible genes in a prokaryote." Kalman M., Murphy H., Cashel M. New Biol. 3:886-895(1991) [PubMed: 1931833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 50-335. Strain: K12.
[7]	"Poly(A) polymerase I of Escherichia coli: characterization of the catalytic domain, an RNA binding site and regions for the interaction with proteins involved in mRNA degradation." Raynal L.C., Carpousis A.J. Mol. Microbiol. 32:765-775(1999) [PubMed: 10361280] [Abstract] Cited for: INTERACTION WITH PCNB.
[8]	"Changes in Escherichia coli transcriptome during acclimatization at low temperature." Polissi A., De Laurentis W., Zangrossi S., Briani F., Longhi V., Pesole G., Deho G. Res. Microbiol. 154:573-580(2003) [PubMed: 14527658] [Abstract] Cited for: INDUCTION BY COLD SHOCK. Strain: K12 / MG1655 / ATCC 47076.
[9]	"Studies on three E. coli DEAD-box helicases point to an unwinding mechanism different from that of model DNA helicases." Bizebard T., Ferlenghi I., Iost I., Dreyfus M. Biochemistry 43:7857-7866(2004) [PubMed: 15196029] [Abstract] Cited for: FUNCTION AS RNA-HELICASE, FUNCTION AS ATPASE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L02123 Genomic DNA. Translation: AAA53653.1. U00096 Genomic DNA. Translation: AAC73884.1. AP009048 Genomic DNA. Translation: BAA35457.1. X56307 Genomic DNA. No translation available.
PIR	E64816.
RefSeq	NP_415318.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P25888.
SMR	P25888. Positions 1-372.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10697N.
IntAct	P25888. 34 interactions.
MINT	MINT-1313387.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000003075; EBESCP00000003075; EBESCG00000002519. EBESCT00000017437; EBESCP00000016728; EBESCG00000016493.
GeneID	945425.
GenomeReviews	Gene locus JW0781 in contig AP009048_GR. Gene locus b0797 in contig U00096_GR.
KEGG	ecj:JW0781. eco:b0797.
PATRIC	32116795. VBIEscCol129921_0823.
Organism-specific databases
EchoBASE	EB1217.
EcoGene	EG11235. rhlE.
Phylogenomic databases
eggNOG	COG0513.
GeneTree	EBGT00050000008320.
HOGENOM	HBG737336.
OMA	GINPQID.
PhylomeDB	P25888.
ProtClustDB	PRK10590.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11235-MONOMER.
Gene expression databases
Genevestigator	P25888.
Family and domain databases
InterPro	IPR014001. DEAD-like_helicase. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR001650. Helicase_C. IPR000629. RNA-helicase_DEAD-box_CS. IPR014014. RNA_helicase_DEAD_Q_motif. [Graphical view]
KO	K11927.
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00039. DEAD_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS51195. Q_MOTIF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RHLE_ECOLI

Accession

Primary (citable) accession number: P25888

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	June 1, 1994
Last modified:	January 25, 2012
This is version 101 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program