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P25869

- UBC_ASFM2

UniProt

P25869 - UBC_ASFM2

Protein

Ubiquitin-conjugating enzyme E2

Gene

UBC

Organism
African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 May 1992)
      Previous versions | rss
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    • Comment

    Functioni

    Performs the second step in the ubiquitination reaction that targets specifically a protein for degradation via the proteasome.By similarity

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei85 – 851Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein ubiquitination Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein
    Ubiquitin-protein ligase
    Gene namesi
    Name:UBC
    Ordered Locus Names:Mal-151
    OrganismiAfrican swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV)
    Taxonomic identifieri10500 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAsfarviridaeAsfivirus
    Virus hostiOrnithodoros (relapsing fever ticks) [TaxID: 6937]
    Phacochoerus aethiopicus (Warthog) [TaxID: 85517]
    Phacochoerus africanus (Warthog) [TaxID: 41426]
    Potamochoerus larvatus (Bushpig) [TaxID: 273792]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000000860: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 213213Ubiquitin-conjugating enzyme E2PRO_0000082592Add
    BLAST

    Expressioni

    Inductioni

    Mainly late in infection.

    Structurei

    3D structure databases

    ProteinModelPortaliP25869.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi183 – 21331Asp/Glu-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25869-1 [UniParc]FASTAAdd to Basket

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    MVSSFLLAEY KNLIVNPSEH FKISVNEDNL TEWDVILKGP PDTLYEGGLF    50
    KAKIVFPPKY PYEPPRLTFT SEMWHPNIYS DGKLCISILH GDNAEEQGMT 100
    WSPAQKIDTV LLSVISLLNE PNPDSPANVD AAKSYRKYLY KEDLESYPME 150
    VKKTVKKSLD ECSAEDIEYF KNVPVNVLPV PSDDYEDEEM EDGTYILTYD 200
    DEDEEEDEEM DDE 213
    Length:213
    Mass (Da):24,468
    Last modified:May 1, 1992 - v1
    Checksum:iF9671BC7385D6DCE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62440 Genomic DNA. Translation: CAA44305.1.
    X71982 Genomic DNA. Translation: CAA50851.1.
    AY261361 Genomic DNA. No translation available.
    PIRiS19158.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62440 Genomic DNA. Translation: CAA44305.1 .
    X71982 Genomic DNA. Translation: CAA50851.1 .
    AY261361 Genomic DNA. No translation available.
    PIRi S19158.

    3D structure databases

    ProteinModelPortali P25869.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A ubiquitin conjugating enzyme encoded by African swine fever virus."
      Hingamp P.M., Arnold J.E., Mayer R.J., Dixon L.K.
      EMBO J. 11:361-366(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "African swine fever virus genomes."
      Kutish G.F., Rock D.L.
      Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiUBC_ASFM2
    AccessioniPrimary (citable) accession number: P25869
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3