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P25867

- UBCD1_DROME

UniProt

P25867 - UBCD1_DROME

Protein

Ubiquitin-conjugating enzyme E2-17 kDa

Gene

eff

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
  1. Functioni

    Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Required for proper telomere behavior during cell divisions and possibly for ubiquitination of proteins involved in postmeiotic stages of spermatogenesis. Deletion mutations are lethal in homozygotes.1 PublicationPROSITE-ProRule annotation

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei85 – 851Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. protein binding Source: UniProtKB
    4. ubiquitin-protein transferase activity Source: FlyBase

    GO - Biological processi

    1. chromosome organization Source: FlyBase
    2. compound eye morphogenesis Source: FlyBase
    3. compound eye photoreceptor cell differentiation Source: FlyBase
    4. cyclin catabolic process Source: FlyBase
    5. female germ-line stem cell asymmetric division Source: FlyBase
    6. germ-line stem cell maintenance Source: FlyBase
    7. male meiosis Source: FlyBase
    8. mitotic nuclear division Source: FlyBase
    9. neurogenesis Source: FlyBase
    10. neuron remodeling Source: FlyBase
    11. oogenesis Source: FlyBase
    12. protein ubiquitination Source: FlyBase
    13. regulation of protein stability Source: FlyBase
    14. regulation of R7 cell differentiation Source: FlyBase
    15. spermatid development Source: FlyBase
    16. ubiquitin-dependent protein catabolic process Source: FlyBase

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Cell cycle, Cell division, Meiosis, Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_181662. Separation of Sister Chromatids.
    REACT_181669. Downregulation of SMAD2/3:SMAD4 transcriptional activity.
    REACT_182058. Senescence-Associated Secretory Phenotype (SASP).
    REACT_210493. Inactivation of APC/C via direct inhibition of the APC/C complex.
    REACT_224351. Regulation of APC/C activators between G1/S and early anaphase.
    REACT_33104. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_92044. APC/C:Cdc20 mediated degradation of Securin.
    SignaLinkiP25867.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2-17 kDa (EC:6.3.2.19)
    Alternative name(s):
    Protein effete
    Ubiquitin carrier protein
    Ubiquitin-protein ligase
    Gene namesi
    Name:eff
    Synonyms:UbcD1
    ORF Names:CG7425
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3R

    Organism-specific databases

    FlyBaseiFBgn0011217. eff.

    Subcellular locationi

    GO - Cellular componenti

    1. microtubule associated complex Source: FlyBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 147147Ubiquitin-conjugating enzyme E2-17 kDaPRO_0000082519Add
    BLAST

    Proteomic databases

    PaxDbiP25867.
    PRIDEiP25867.

    Expressioni

    Gene expression databases

    BgeeiP25867.

    Interactioni

    Protein-protein interaction databases

    BioGridi66859. 28 interactions.
    DIPiDIP-18953N.
    IntActiP25867. 6 interactions.
    MINTiMINT-1014718.
    STRINGi7227.FBpp0082477.

    Structurei

    3D structure databases

    ProteinModelPortaliP25867.
    SMRiP25867. Positions 1-147.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    GeneTreeiENSGT00740000115162.
    InParanoidiP25867.
    KOiK06689.
    OMAiNDLGRDP.
    OrthoDBiEOG7PCJGX.
    PhylomeDBiP25867.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25867-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALKRINKEL QDLGRDPPAQ CSAGPVGDDL FHWQATIMGP PDSPYQGGVF    50
    FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS 100
    KVLLSICSLL CDPNPDDPLV PEIARIYKTD REKYNELARE WTRKYAM 147
    Length:147
    Mass (Da):16,678
    Last modified:May 1, 1992 - v1
    Checksum:i82E0CE24F925E2EE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62575 mRNA. Translation: CAA44453.1.
    AE014297 Genomic DNA. Translation: AAF55093.1.
    AY060304 mRNA. Translation: AAL25343.1.
    U68298 mRNA. Translation: AAB39622.1.
    PIRiS19157.
    RefSeqiNP_001262578.1. NM_001275649.1.
    NP_001262579.1. NM_001275650.1.
    NP_731941.1. NM_169601.2.
    UniGeneiDm.4048.

    Genome annotation databases

    EnsemblMetazoaiFBtr0083018; FBpp0082477; FBgn0011217.
    FBtr0310554; FBpp0302691; FBgn0011217.
    GeneIDi41785.
    KEGGidme:Dmel_CG7425.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X62575 mRNA. Translation: CAA44453.1 .
    AE014297 Genomic DNA. Translation: AAF55093.1 .
    AY060304 mRNA. Translation: AAL25343.1 .
    U68298 mRNA. Translation: AAB39622.1 .
    PIRi S19157.
    RefSeqi NP_001262578.1. NM_001275649.1.
    NP_001262579.1. NM_001275650.1.
    NP_731941.1. NM_169601.2.
    UniGenei Dm.4048.

    3D structure databases

    ProteinModelPortali P25867.
    SMRi P25867. Positions 1-147.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 66859. 28 interactions.
    DIPi DIP-18953N.
    IntActi P25867. 6 interactions.
    MINTi MINT-1014718.
    STRINGi 7227.FBpp0082477.

    Proteomic databases

    PaxDbi P25867.
    PRIDEi P25867.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0083018 ; FBpp0082477 ; FBgn0011217 .
    FBtr0310554 ; FBpp0302691 ; FBgn0011217 .
    GeneIDi 41785.
    KEGGi dme:Dmel_CG7425.

    Organism-specific databases

    CTDi 41785.
    FlyBasei FBgn0011217. eff.

    Phylogenomic databases

    eggNOGi COG5078.
    GeneTreei ENSGT00740000115162.
    InParanoidi P25867.
    KOi K06689.
    OMAi NDLGRDP.
    OrthoDBi EOG7PCJGX.
    PhylomeDBi P25867.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_181662. Separation of Sister Chromatids.
    REACT_181669. Downregulation of SMAD2/3:SMAD4 transcriptional activity.
    REACT_182058. Senescence-Associated Secretory Phenotype (SASP).
    REACT_210493. Inactivation of APC/C via direct inhibition of the APC/C complex.
    REACT_224351. Regulation of APC/C activators between G1/S and early anaphase.
    REACT_33104. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_92044. APC/C:Cdc20 mediated degradation of Securin.
    SignaLinki P25867.

    Miscellaneous databases

    ChiTaRSi eff. drosophila.
    GenomeRNAii 41785.
    NextBioi 825564.

    Gene expression databases

    Bgeei P25867.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Drosophila UbcD1 encodes a highly conserved ubiquitin-conjugating enzyme involved in selective protein degradation."
      Treier M., Seufert W., Jentsch S.
      EMBO J. 11:367-372(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Oregon-P2.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Head.
    5. "UbcD1, a Drosophila ubiquitin-conjugating enzyme required for proper telomere behavior."
      Cenci G., Rawson R.B., Belloni G., Castrillon D.H., Tudor M., Petrucci R., Goldberg M.L., Wasserman S.A., Gatti M.
      Genes Dev. 11:863-875(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-11, FUNCTION.

    Entry informationi

    Entry nameiUBCD1_DROME
    AccessioniPrimary (citable) accession number: P25867
    Secondary accession number(s): Q9VFG6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3