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P25867

- UBCD1_DROME

UniProt

P25867 - UBCD1_DROME

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Protein

Ubiquitin-conjugating enzyme E2-17 kDa

Gene

eff

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Required for proper telomere behavior during cell divisions and possibly for ubiquitination of proteins involved in postmeiotic stages of spermatogenesis. Deletion mutations are lethal in homozygotes.1 PublicationPROSITE-ProRule annotation

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. ubiquitin-protein transferase activity Source: FlyBase

GO - Biological processi

  1. chromosome organization Source: FlyBase
  2. compound eye morphogenesis Source: FlyBase
  3. compound eye photoreceptor cell differentiation Source: FlyBase
  4. cyclin catabolic process Source: FlyBase
  5. female germ-line stem cell asymmetric division Source: FlyBase
  6. germ-line stem cell maintenance Source: FlyBase
  7. male meiosis Source: FlyBase
  8. mitotic nuclear division Source: FlyBase
  9. neurogenesis Source: FlyBase
  10. neuron remodeling Source: FlyBase
  11. oogenesis Source: FlyBase
  12. protein polyubiquitination Source: FlyBase
  13. protein ubiquitination Source: FlyBase
  14. regulation of protein stability Source: FlyBase
  15. regulation of R7 cell differentiation Source: FlyBase
  16. spermatid development Source: FlyBase
  17. ubiquitin-dependent protein catabolic process Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Meiosis, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_181662. Separation of Sister Chromatids.
REACT_181669. Downregulation of SMAD2/3:SMAD4 transcriptional activity.
REACT_182058. Senescence-Associated Secretory Phenotype (SASP).
REACT_210493. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_224351. Regulation of APC/C activators between G1/S and early anaphase.
REACT_33104. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_92044. APC/C:Cdc20 mediated degradation of Securin.
SignaLinkiP25867.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2-17 kDa (EC:6.3.2.19)
Alternative name(s):
Protein effete
Ubiquitin carrier protein
Ubiquitin-protein ligase
Gene namesi
Name:eff
Synonyms:UbcD1
ORF Names:CG7425
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0011217. eff.

Subcellular locationi

GO - Cellular componenti

  1. microtubule associated complex Source: FlyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 147147Ubiquitin-conjugating enzyme E2-17 kDaPRO_0000082519Add
BLAST

Proteomic databases

PaxDbiP25867.
PRIDEiP25867.

Expressioni

Gene expression databases

BgeeiP25867.

Interactioni

Protein-protein interaction databases

BioGridi66859. 28 interactions.
DIPiDIP-18953N.
IntActiP25867. 6 interactions.
MINTiMINT-1014718.
STRINGi7227.FBpp0082477.

Structurei

3D structure databases

ProteinModelPortaliP25867.
SMRiP25867. Positions 1-147.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00760000119012.
InParanoidiP25867.
KOiK06689.
OMAiNDLGRDP.
OrthoDBiEOG7PCJGX.
PhylomeDBiP25867.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25867-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALKRINKEL QDLGRDPPAQ CSAGPVGDDL FHWQATIMGP PDSPYQGGVF
60 70 80 90 100
FLTIHFPTDY PFKPPKVAFT TRIYHPNINS NGSICLDILR SQWSPALTIS
110 120 130 140
KVLLSICSLL CDPNPDDPLV PEIARIYKTD REKYNELARE WTRKYAM
Length:147
Mass (Da):16,678
Last modified:May 1, 1992 - v1
Checksum:i82E0CE24F925E2EE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X62575 mRNA. Translation: CAA44453.1.
AE014297 Genomic DNA. Translation: AAF55093.1.
AY060304 mRNA. Translation: AAL25343.1.
U68298 mRNA. Translation: AAB39622.1.
PIRiS19157.
RefSeqiNP_001262578.1. NM_001275649.1.
NP_001262579.1. NM_001275650.1.
NP_731941.1. NM_169601.2.
UniGeneiDm.4048.

Genome annotation databases

EnsemblMetazoaiFBtr0083018; FBpp0082477; FBgn0011217.
FBtr0310554; FBpp0302691; FBgn0011217.
GeneIDi41785.
KEGGidme:Dmel_CG7425.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X62575 mRNA. Translation: CAA44453.1 .
AE014297 Genomic DNA. Translation: AAF55093.1 .
AY060304 mRNA. Translation: AAL25343.1 .
U68298 mRNA. Translation: AAB39622.1 .
PIRi S19157.
RefSeqi NP_001262578.1. NM_001275649.1.
NP_001262579.1. NM_001275650.1.
NP_731941.1. NM_169601.2.
UniGenei Dm.4048.

3D structure databases

ProteinModelPortali P25867.
SMRi P25867. Positions 1-147.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 66859. 28 interactions.
DIPi DIP-18953N.
IntActi P25867. 6 interactions.
MINTi MINT-1014718.
STRINGi 7227.FBpp0082477.

Proteomic databases

PaxDbi P25867.
PRIDEi P25867.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0083018 ; FBpp0082477 ; FBgn0011217 .
FBtr0310554 ; FBpp0302691 ; FBgn0011217 .
GeneIDi 41785.
KEGGi dme:Dmel_CG7425.

Organism-specific databases

CTDi 41785.
FlyBasei FBgn0011217. eff.

Phylogenomic databases

eggNOGi COG5078.
GeneTreei ENSGT00760000119012.
InParanoidi P25867.
KOi K06689.
OMAi NDLGRDP.
OrthoDBi EOG7PCJGX.
PhylomeDBi P25867.

Enzyme and pathway databases

UniPathwayi UPA00143 .
Reactomei REACT_181662. Separation of Sister Chromatids.
REACT_181669. Downregulation of SMAD2/3:SMAD4 transcriptional activity.
REACT_182058. Senescence-Associated Secretory Phenotype (SASP).
REACT_210493. Inactivation of APC/C via direct inhibition of the APC/C complex.
REACT_224351. Regulation of APC/C activators between G1/S and early anaphase.
REACT_33104. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_92044. APC/C:Cdc20 mediated degradation of Securin.
SignaLinki P25867.

Miscellaneous databases

ChiTaRSi eff. drosophila.
GenomeRNAii 41785.
NextBioi 825564.

Gene expression databases

Bgeei P25867.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Drosophila UbcD1 encodes a highly conserved ubiquitin-conjugating enzyme involved in selective protein degradation."
    Treier M., Seufert W., Jentsch S.
    EMBO J. 11:367-372(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Oregon-P2.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Head.
  5. "UbcD1, a Drosophila ubiquitin-conjugating enzyme required for proper telomere behavior."
    Cenci G., Rawson R.B., Belloni G., Castrillon D.H., Tudor M., Petrucci R., Goldberg M.L., Wasserman S.A., Gatti M.
    Genes Dev. 11:863-875(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-11, FUNCTION.

Entry informationi

Entry nameiUBCD1_DROME
AccessioniPrimary (citable) accession number: P25867
Secondary accession number(s): Q9VFG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: October 29, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3