ID UBC1_ARATH Reviewed; 152 AA. AC P25865; Q4TZ08; DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot. DT 01-MAY-1992, sequence version 1. DT 16-JUN-2009, entry version 85. DE RecName: Full=Ubiquitin-conjugating enzyme E2 1; DE EC=6.3.2.19; DE AltName: Full=Ubiquitin-conjugating enzyme E2-17 kDa 1; DE AltName: Full=Ubiquitin-protein ligase 1; DE AltName: Full=Ubiquitin carrier protein 1; GN Name=UBC1; OrderedLocusNames=At1g14400; ORFNames=F14L17.17, F14L17_35; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; OC rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RC STRAIN=cv. Columbia; RX MEDLINE=92084684; PubMed=1660887; RA Sullivan M.L., Vierstra R.D.; RT "Cloning of a 16-kDa ubiquitin carrier protein from wheat and RT Arabidopsis thaliana. Identification of functional domains by in vitro RT mutagenesis."; RL J. Biol. Chem. 266:23878-23885(1991). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=cv. Columbia; RX MEDLINE=94207190; PubMed=8155884; DOI=10.1007/BF00023561; RA Sullivan M.L., Carpenter T.B., Vierstra R.D.; RT "Homologues of wheat ubiquitin-conjugating enzymes -- TaUBC1 and RT TaUBC4 are encoded by small multigene families in Arabidopsis RT thaliana."; RL Plant Mol. Biol. 24:651-661(1994). RN [3] RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, GENE FAMILY, RP AND NOMENCLATURE. RX PubMed=16339806; DOI=10.1104/pp.105.067983; RA Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., RA Callis J.; RT "Genome analysis and functional characterization of the E2 and RING- RT type E3 ligase ubiquitination enzymes of Arabidopsis."; RL Plant Physiol. 139:1597-1611(2005). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX MEDLINE=21016719; PubMed=11130712; DOI=10.1038/35048500; RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., RA White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., RA Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., RA Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., RA Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., RA Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., RA Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., RA Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., RA Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., RA Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., RA Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., RA Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., RA Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., RA Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., RA Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.; RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis RT thaliana."; RL Nature 408:816-820(2000). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RX MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305; RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., RA Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., RA Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., RA Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., RA Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., RA Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., RA Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., RA Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., RA Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., RA Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., RA Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., RA Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.; RT "Empirical analysis of transcriptional activity in the Arabidopsis RT genome."; RL Science 302:842-846(2003). RN [6] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., RA Feldmann K.A.; RT "Full-length cDNA from Arabidopsis thaliana."; RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases. RN [7] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K., RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., RA Hayashizaki Y., Shinozaki K.; RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."; RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases. RN [8] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-98. RC STRAIN=cv. Columbia; TISSUE=Dry seed; RX MEDLINE=96158348; PubMed=8580968; RX DOI=10.1046/j.1365-313X.1996.09010101.x; RA Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C., RA Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R., RA Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., RA Marbach J., Fleck J., Clement B., Philipps G., Herve C., Bardet C., RA Tremousaygue D., Lescure B., Lacomme C., Roby D., Jourjon M.-F., RA Chabrier P., Charpenteau J.-L., Desprez T., Amselem J., Chiapello H., RA Hoefte H.; RT "Further progress towards a catalogue of all Arabidopsis genes: RT analysis of a set of 5000 non-redundant ESTs."; RL Plant J. 9:101-124(1996). RN [9] RP FUNCTION, AND MUTAGENESIS OF CYS-88. RX PubMed=8386169; RA Sullivan M.L., Vierstra R.D.; RT "Formation of a stable adduct between ubiquitin and the Arabidopsis RT ubiquitin-conjugating enzyme, AtUBC1+."; RL J. Biol. Chem. 268:8777-8780(1993). RN [10] RP TISSUE SPECIFICITY, AND INDUCTION. RX PubMed=8790283; DOI=10.1007/BF00042223; RA Thoma S., Sullivan M.L., Vierstra R.D.; RT "Members of two gene families encoding ubiquitin-conjugating enzymes, RT AtUBC1-3 and AtUBC4-6, from Arabidopsis thaliana are differentially RT expressed."; RL Plant Mol. Biol. 31:493-505(1996). RN [11] RP X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS). RX MEDLINE=92340563; PubMed=1321826; RA Cook W.J., Jeffrey L.C., Sullivan M.L., Vierstra R.D.; RT "Three-dimensional structure of a ubiquitin-conjugating enzyme (E2)."; RL J. Biol. Chem. 267:15116-15121(1992). CC -!- FUNCTION: Catalyzes the covalent attachment of ubiquitin to other CC proteins. CC -!- CATALYTIC ACTIVITY: ATP + ubiquitin + protein lysine = AMP + CC diphosphate + protein N-ubiquityllysine. CC -!- PATHWAY: Protein modification; protein ubiquitination. CC -!- TISSUE SPECIFICITY: Ubiquitously expressed. CC -!- INDUCTION: Not induced by heat shock. CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. CC -!- WEB RESOURCE: Name=PlantsUBQ; Note=A functional genomics database CC for the ubiquitin/26S proteasome proteolytic pathway in plants; CC URL="http://plantsubq.genomics.purdue.edu/"; CC ----------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution-NoDerivs License CC ----------------------------------------------------------------------- DR EMBL; M62721; AAA32903.1; -; mRNA. DR EMBL; L19351; AAA32897.1; -; Genomic_DNA. DR EMBL; DQ027016; AAY44842.1; -; mRNA. DR EMBL; AC012188; AAF43940.1; -; Genomic_DNA. DR EMBL; AF332451; AAG48814.1; -; mRNA. DR EMBL; AY070074; AAL49769.1; -; mRNA. DR EMBL; AY091330; AAM14269.1; -; mRNA. DR EMBL; AY085783; AAM63000.1; -; mRNA. DR EMBL; AK226391; BAE98537.1; -; mRNA. DR EMBL; Z27262; CAA81773.1; -; mRNA. DR IPI; IPI00524846; -. DR PIR; S43781; S43781. DR RefSeq; NP_563951.1; -. DR RefSeq; NP_973825.1; -. DR UniGene; At.331; -. DR PDB; 2AAK; X-ray; 2.40 A; A=1-152. DR PDBsum; 2AAK; -. DR IntAct; P25865; 2. DR PRIDE; P25865; -. DR GeneID; 838002; -. DR GenomeReviews; CT485782_GR; AT1G14400. DR KEGG; ath:AT1G14400; -. DR GeneFarm; 4743; 464. DR TAIR; At1g14400; -. DR OMA; P25865; EQSWVDS. DR BRENDA; 6.3.2.19; 302. DR ArrayExpress; P25865; -. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0004842; F:ubiquitin-protein ligase activity; IDA:TAIR. DR GO; GO:0033523; P:histone H2B ubiquitination; IGI:TAIR. DR GO; GO:0009965; P:leaf morphogenesis; IGI:TAIR. DR GO; GO:0009910; P:negative regulation of flower development; IGI:TAIR. DR GO; GO:0051246; P:regulation of protein metabolic process; IEA:InterPro. DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IDA:TAIR. DR GO; GO:0010228; P:vegetative to reproductive phase transition; IGI:TAIR. DR InterPro; IPR016135; UBQ-conjugat/RWD-like. DR InterPro; IPR000608; UBQ-conjugat_E2. DR Gene3D; G3DSA:3.10.110.10; UBQ-conjugat_E2; 1. DR Pfam; PF00179; UQ_con; 1. DR ProDom; PD000461; UBQ_conjugat; 1. DR SMART; SM00212; UBCc; 1. DR PROSITE; PS00183; UBIQUITIN_CONJUGAT_1; 1. DR PROSITE; PS50127; UBIQUITIN_CONJUGAT_2; 1. PE 1: Evidence at protein level; KW 3D-structure; ATP-binding; Complete proteome; Ligase; KW Nucleotide-binding; Ubl conjugation pathway. FT CHAIN 1 152 Ubiquitin-conjugating enzyme E2 1. FT /FTId=PRO_0000082570. FT ACT_SITE 88 88 Glycyl thioester intermediate. FT MUTAGEN 88 88 C->S: Stabilization of the ester bond FT with the ubiquitin. FT HELIX 4 18 FT STRAND 24 29 FT STRAND 32 41 FT TURN 47 50 FT STRAND 52 58 FT TURN 61 65 FT STRAND 69 74 FT STRAND 85 87 FT HELIX 90 92 FT HELIX 102 113 FT HELIX 124 132 FT HELIX 134 148 SQ SEQUENCE 152 AA; 17281 MW; 896D911930C39045 CRC64; MSTPARKRLM RDFKRLQQDP PAGISGAPQD NNIMLWNAVI FGPDDTPWDG GTFKLSLQFS EDYPNKPPTV RFVSRMFHPN IYADGSICLD ILQNQWSPIY DVAAILTSIQ SLLCDPNPNS PANSEAARMY SESKREYNRR VRDVVEQSWT AD //