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Protein

Ubiquitin-conjugating enzyme E2 1

Gene

UBC1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accepts the ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins.2 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei88Glycyl thioester intermediate1

GO - Molecular functioni

GO - Biological processi

  • DNA repair Source: GO_Central
  • histone H2B ubiquitination Source: TAIR
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
  • protein polyubiquitination Source: GO_Central
  • ubiquitin-dependent protein catabolic process Source: TAIR
  • vegetative to reproductive phase transition of meristem Source: TAIR

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 1 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 1
Ubiquitin carrier protein 1
Ubiquitin-conjugating enzyme E2-17 kDa 1
Ubiquitin-protein ligase 1
Gene namesi
Name:UBC1
Ordered Locus Names:At1g14400
ORF Names:F14L17.17, F14L17_35
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G14400
TAIRilocus:2012622 AT1G14400

Subcellular locationi

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi88C → S: Stabilization of the ester bond with the ubiquitin. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825701 – 152Ubiquitin-conjugating enzyme E2 1Add BLAST152

Proteomic databases

PaxDbiP25865
PRIDEiP25865

Expressioni

Tissue specificityi

Ubiquitously expressed.2 Publications

Inductioni

Not induced by heat shock.1 Publication

Gene expression databases

ExpressionAtlasiP25865 baseline and differential
GenevisibleiP25865 AT

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi23242, 3 interactors
IntActiP25865, 2 interactors
STRINGi3702.AT1G14400.1

Structurei

Secondary structure

1152
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 18Combined sources15
Beta strandi24 – 29Combined sources6
Beta strandi32 – 41Combined sources10
Turni47 – 50Combined sources4
Beta strandi52 – 58Combined sources7
Turni61 – 65Combined sources5
Beta strandi69 – 74Combined sources6
Beta strandi85 – 87Combined sources3
Helixi90 – 92Combined sources3
Helixi102 – 113Combined sources12
Helixi124 – 132Combined sources9
Helixi134 – 148Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AAKX-ray2.40A1-152[»]
ProteinModelPortaliP25865
SMRiP25865
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25865

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0419 Eukaryota
COG5078 LUCA
HOGENOMiHOG000233454
InParanoidiP25865
KOiK10573
OMAiDFKXPEG
OrthoDBiEOG09360PJJ
PhylomeDBiP25865

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

P25865-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPARKRLM RDFKRLQQDP PAGISGAPQD NNIMLWNAVI FGPDDTPWDG
60 70 80 90 100
GTFKLSLQFS EDYPNKPPTV RFVSRMFHPN IYADGSICLD ILQNQWSPIY
110 120 130 140 150
DVAAILTSIQ SLLCDPNPNS PANSEAARMY SESKREYNRR VRDVVEQSWT

AD
Length:152
Mass (Da):17,281
Last modified:May 1, 1992 - v1
Checksum:i896D911930C39045
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62721 mRNA Translation: AAA32903.1
L19351 Genomic DNA Translation: AAA32897.1
DQ027016 mRNA Translation: AAY44842.1
AC012188 Genomic DNA Translation: AAF43940.1
CP002684 Genomic DNA Translation: AEE29158.1
CP002684 Genomic DNA Translation: AEE29159.1
AF332451 mRNA Translation: AAG48814.1
AY070074 mRNA Translation: AAL49769.1
AY091330 mRNA Translation: AAM14269.1
AY085783 mRNA Translation: AAM63000.1
AK226391 mRNA Translation: BAE98537.1
Z27262 mRNA Translation: CAA81773.1
PIRiS43781
RefSeqiNP_563951.1, NM_101307.5
NP_973825.1, NM_202096.2
UniGeneiAt.331

Genome annotation databases

EnsemblPlantsiAT1G14400.1; AT1G14400.1; AT1G14400
AT1G14400.2; AT1G14400.2; AT1G14400
GeneIDi838002
GrameneiAT1G14400.1; AT1G14400.1; AT1G14400
AT1G14400.2; AT1G14400.2; AT1G14400
KEGGiath:AT1G14400

Similar proteinsi

Entry informationi

Entry nameiUBC1_ARATH
AccessioniPrimary (citable) accession number: P25865
Secondary accession number(s): Q4TZ08
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: May 23, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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