Reviewed, UniProtKB/Swiss-Prot P25865 (UBC1_ARATH)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 1
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-conjugating enzyme E2-17 kDa 1
    Ubiquitin-protein ligase 1
    Ubiquitin carrier protein 1

Gene names

Name:	UBC1
Ordered Locus Names:	At1g14400
ORF Names:	F14L17.17, F14L17_35

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Ref.3 Ref.9
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Tissue specificity	Ubiquitously expressed. Ref.3 Ref.10
Induction	Not induced by heat shock. Ref.10
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	histone H2B ubiquitination Inferred from genetic interaction. Source: TAIR leaf morphogenesis Inferred from genetic interaction. Source: TAIR negative regulation of flower development Inferred from genetic interaction. Source: TAIR regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro ubiquitin-dependent protein catabolic process Ref.3 Inferred from direct assay. Source: TAIR vegetative to reproductive phase transition Inferred from genetic interaction. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Ref.3 Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 152

152

Ubiquitin-conjugating enzyme E2 1

PRO_0000082570

Sites

Active site

88

1

Glycyl thioester intermediate

Experimental info

Mutagenesis

88

1

C → S: Stabilization of the ester bond with the ubiquitin. Ref.9

Secondary structure

1

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152

Helix Strand Turn

Details...

Helix

4 – 18

15

Beta strand

24 – 29

6

Beta strand

32 – 41

10

Turn

47 – 50

4

Beta strand

52 – 58

7

Turn

61 – 65

5

Beta strand

69 – 74

6

Beta strand

85 – 87

3

Helix

90 – 92

3

Helix

102 – 113

12

Helix

124 – 132

9

Helix

134 – 148

15

Sequences

	Sequence		Length	Mass (Da)
	P25865-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 896D911930C39045	FASTA	152	17,281
	10 20 30 40 50 60 MSTPARKRLM RDFKRLQQDP PAGISGAPQD NNIMLWNAVI FGPDDTPWDG GTFKLSLQFS 70 80 90 100 110 120 EDYPNKPPTV RFVSRMFHPN IYADGSICLD ILQNQWSPIY DVAAILTSIQ SLLCDPNPNS 130 140 150 PANSEAARMY SESKREYNRR VRDVVEQSWT AD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a 16-kDa ubiquitin carrier protein from wheat and Arabidopsis thaliana. Identification of functional domains by in vitro mutagenesis." Sullivan M.L., Vierstra R.D. J. Biol. Chem. 266:23878-23885(1991) [PubMed: 1660887] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Homologues of wheat ubiquitin-conjugating enzymes -- TaUBC1 and TaUBC4 are encoded by small multigene families in Arabidopsis thaliana." Sullivan M.L., Carpenter T.B., Vierstra R.D. Plant Mol. Biol. 24:651-661(1994) [PubMed: 8155884] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Genome analysis and functional characterization of the E2 and RING-type E3 ligase ubiquitination enzymes of Arabidopsis." Kraft E., Stone S.L., Ma L., Su N., Gao Y., Lau O.-S., Deng X.-W., Callis J. Plant Physiol. 139:1597-1611(2005) [PubMed: 16339806] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
[4]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[8]	"Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non-redundant ESTs." Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C., Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R., Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J. , Fleck J., Clement B., Philipps G., Herve C., Bardet C., Tremousaygue D., Lescure B., Lacomme C., Roby D., Jourjon M.-F., Chabrier P., Charpenteau J.-L., Desprez T., Amselem J., Chiapello H., Hoefte H. Plant J. 9:101-124(1996) [PubMed: 8580968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-98. Strain: cv. Columbia. Tissue: Dry seed.
[9]	"Formation of a stable adduct between ubiquitin and the Arabidopsis ubiquitin-conjugating enzyme, AtUBC1+." Sullivan M.L., Vierstra R.D. J. Biol. Chem. 268:8777-8780(1993) [PubMed: 8386169] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF CYS-88.
[10]	"Members of two gene families encoding ubiquitin-conjugating enzymes, AtUBC1-3 and AtUBC4-6, from Arabidopsis thaliana are differentially expressed." Thoma S., Sullivan M.L., Vierstra R.D. Plant Mol. Biol. 31:493-505(1996) [PubMed: 8790283] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION.
[11]	"Three-dimensional structure of a ubiquitin-conjugating enzyme (E2)." Cook W.J., Jeffrey L.C., Sullivan M.L., Vierstra R.D. J. Biol. Chem. 267:15116-15121(1992) [PubMed: 1321826] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).

Web resources

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Cross-references

Sequence databases

EMBLGenBankDDBJ

M62721 mRNA. Translation: AAA32903.1.
L19351 Genomic DNA. Translation: AAA32897.1.
DQ027016 mRNA. Translation: AAY44842.1.
AC012188 Genomic DNA. Translation: AAF43940.1.
AF332451 mRNA. Translation: AAG48814.1.
AY070074 mRNA. Translation: AAL49769.1.
AY091330 mRNA. Translation: AAM14269.1.
AY085783 mRNA. Translation: AAM63000.1.
AK226391 mRNA. Translation: BAE98537.1.
Z27262 mRNA. Translation: CAA81773.1.

IPI

IPI00524846.

PIR

S43781.

RefSeq

NP_563951.1.
NP_973825.1.

UniGene

At.331

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2AAK	X-ray	2.40	A	1-152	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P25865. 2 interactions.

Proteomic databases

PRIDE

P25865.

Genome annotation databases

GeneID

838002.

GenomeReviews

Gene locus AT1G14400 in contig CT485782_GR.

KEGG

ath:AT1G14400.

Organism-specific databases

GeneFarm

4743. 464.

TAIR

At1g14400.

Phylogenomic databases

OMA

P25865. EQSWVDS.

Enzyme and pathway databases

BRENDA

6.3.2.19. 302.

Gene expression databases

ArrayExpress

P25865.

Family and domain databases

InterPro

IPR016135. UBQ-conjugat/RWD-like.
IPR000608. UBQ-conjugat_E2.
[Graphical view]

Gene3D

G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.

Pfam

PF00179. UQ_con. 1 hit.
[Graphical view]

ProDom

PD000461. UBQ_conjugat. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00212. UBCc. 1 hit.
[Graphical view]

PROSITE

PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

UBC1_ARATH

Accession

Primary (citable) accession number: P25865
Secondary accession number(s): Q4TZ08

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	June 16, 2009
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)