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P25859

- CKB11_ARATH

UniProt

P25859 - CKB11_ARATH

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Protein
Cyclin-dependent kinase B1-1
Gene
CDKB1-1, CDC2B, At3g54180, F24B22.140
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May control G2/M (mitosis) phase progression. Plays a role in regulating seedling growth in darkness via regulation of hypocotyl cell elongation and cotyledon cell development. Plays a role in stomatal development. Required to suppress endoreduplication.5 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulationi

Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-176 activates it By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331ATP By similarity
Active sitei142 – 1421Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC
  3. cyclin-dependent protein serine/threonine kinase activity Source: TAIR
  4. protein binding Source: IntAct

GO - Biological processi

  1. DNA endoreduplication Source: TAIR
  2. cotyledon development Source: TAIR
  3. mitotic nuclear division Source: UniProtKB-KW
  4. stomatal complex formation Source: TAIR
  5. unidimensional cell growth Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G54180-MONOMER.
BRENDAi2.7.11.22. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase B1-1 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDKB1;1
Alternative name(s):
Cell division control protein 2 homolog B
Gene namesi
Name:CDKB1-1
Synonyms:CDC2B
Ordered Locus Names:At3g54180
ORF Names:F24B22.140
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G54180.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: TAIR
  2. nucleus Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi14 – 141T → A: Decreased kinase activity and disturbed cell cycle; when associated with F-15. 1 Publication
Mutagenesisi15 – 151Y → F: Decreased kinase activity and disturbed cell cycle; when associated with A-14. 1 Publication
Mutagenesisi161 – 1611D → N: Dominant negative. Decreased kinase activity and disturbed cell cycle. 3 Publications
Mutagenesisi171 – 1711P → L: Decreased kinase activity and disturbed cell cycle. 1 Publication
Mutagenesisi181 – 1811T → I: Decreased kinase activity and disturbed cell cycle. 1 Publication
Mutagenesisi249 – 2513Missing: Decreased kinase activity and disturbed cell cycle. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309Cyclin-dependent kinase B1-1
PRO_0000085750Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei15 – 151Phosphotyrosine By similarity
Modified residuei176 – 1761Phosphothreonine; by CAK By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP25859.
PRIDEiP25859.

Expressioni

Tissue specificityi

Highly expressed in guard cells and stomatal precursor cells of cotyledons. Expressed in roots, stems, flowers and siliques.2 Publications

Developmental stagei

Preferentially expressed in the S and G2 phases. Expressed in actively dividing cells: root and shoot apical meristems, leaf primordia and emerging lateral root meristem. Expressed in light-grown seedlings from 1 up to 7 days after germination with a peak at 2 and 3 days.3 Publications

Gene expression databases

GenevestigatoriP25859.

Interactioni

Subunit structurei

Interacts with CKS1 and CYCU3-1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CKS1O232497EBI-1253311,EBI-1253127

Protein-protein interaction databases

BioGridi9902. 47 interactions.
IntActiP25859. 22 interactions.
STRINGi3702.AT3G54180.1-P.

Structurei

3D structure databases

ProteinModelPortaliP25859.
SMRiP25859. Positions 1-306.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 301298Protein kinase
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiP25859.
KOiK07760.
OMAiRDWHEFP.
PhylomeDBiP25859.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25859-1 [UniParc]FASTAAdd to Basket

« Hide

MEKYEKLEKV GEGTYGKVYK AMEKGTGKLV ALKKTRLEMD EEGIPPTALR    50
EISLLQMLST SIYVVRLLCV EHVHQPSTKS QSTKSNLYLV FEYLDTDLKK 100
FIDSYRKGPN PKPLEPFLIQ KLMFQLCKGV AHCHSHGVLH RDLKPQNLLL 150
VKDKELLKIA DLGLGRAFTV PLKSYTHEIV TLWYRAPEVL LGSTHYSTGV 200
DMWSVGCIFA EMVRRQALFP GDSEFQQLLH IFRLLGTPTE QQWPGVSTLR 250
DWHVYPKWEP QDLTLAVPSL SPQGVDLLTK MLKYNPAERI SAKTALDHPY 300
FDSLDKSQF 309
Length:309
Mass (Da):35,318
Last modified:November 1, 1995 - v2
Checksum:i8FFCD006A9A4A75B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti226 – 2261Q → R in CAA40972. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10851 Genomic DNA. Translation: BAA01624.1.
AL132957 Genomic DNA. Translation: CAB70992.1.
CP002686 Genomic DNA. Translation: AEE79196.1.
BT026079 mRNA. Translation: ABG48435.1.
AY084810 mRNA. Translation: AAM61376.1.
X57840 mRNA. Translation: CAA40972.1.
PIRiS23096.
RefSeqiNP_190986.1. NM_115278.2.
UniGeneiAt.10.

Genome annotation databases

EnsemblPlantsiAT3G54180.1; AT3G54180.1; AT3G54180.
GeneIDi824585.
KEGGiath:AT3G54180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10851 Genomic DNA. Translation: BAA01624.1 .
AL132957 Genomic DNA. Translation: CAB70992.1 .
CP002686 Genomic DNA. Translation: AEE79196.1 .
BT026079 mRNA. Translation: ABG48435.1 .
AY084810 mRNA. Translation: AAM61376.1 .
X57840 mRNA. Translation: CAA40972.1 .
PIRi S23096.
RefSeqi NP_190986.1. NM_115278.2.
UniGenei At.10.

3D structure databases

ProteinModelPortali P25859.
SMRi P25859. Positions 1-306.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 9902. 47 interactions.
IntActi P25859. 22 interactions.
STRINGi 3702.AT3G54180.1-P.

Proteomic databases

PaxDbi P25859.
PRIDEi P25859.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G54180.1 ; AT3G54180.1 ; AT3G54180 .
GeneIDi 824585.
KEGGi ath:AT3G54180.

Organism-specific databases

GeneFarmi 2951. 107.
TAIRi AT3G54180.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.
InParanoidi P25859.
KOi K07760.
OMAi RDWHEFP.
PhylomeDBi P25859.

Enzyme and pathway databases

BioCyci ARA:AT3G54180-MONOMER.
BRENDAi 2.7.11.22. 399.

Miscellaneous databases

PROi P25859.

Gene expression databases

Genevestigatori P25859.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Exon-intron organization of the Arabidopsis thaliana protein kinase genes CDC2a and CDC2b."
    Imajuku Y., Hirayama T., Endoh H., Oka A.
    FEBS Lett. 304:73-77(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Arabidopsis ORF clones."
    Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Identification of two cell-cycle-controlling cdc2 gene homologs in Arabidopsis thaliana."
    Hirayama T., Imajuku Y., Anai T., Matsui M., Oka A.
    Gene 105:159-165(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 140-309.
    Strain: cv. Columbia.
  7. "The Arabidopsis cyclin-dependent kinase gene cdc2bAt is preferentially expressed during S and G2 phases of the cell cycle."
    Segers G., Gadisseur I., Bergounioux C., de Almeida Engler J., Jacqmard A., van Montagu M., Inze D.
    Plant J. 10:601-612(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  8. "The Arabidopsis Cks1At protein binds the cyclin-dependent kinases Cdc2aAt and Cdc2bAt."
    de Veylder L., Segers G., Glab N., Casteels P., van Montagu M., Inze D.
    FEBS Lett. 412:446-452(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CKS1.
  9. "Mutational analysis of two Arabidopsis thaliana cyclin-dependent kinases in fission yeast."
    Porceddu A., de Veylder L., Hayles J., van Montagu M., Inze D., Mironov V.
    FEBS Lett. 446:182-188(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF THR-14; TYR-15; ASP-161; PRO-171; THR-181 AND 249-LEU--ASP-251.
  10. "An Arabidopsis cell cycle-dependent kinase-related gene, CDC2b, plays a role in regulating seedling growth in darkness."
    Yoshizumi T., Nagata N., Shimada H., Matsui M.
    Plant Cell 11:1883-1896(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.
  11. "An upstream region of the Arabidopsis thaliana CDKA;1 (CDC2aAt) gene directs transcription during trichome development."
    Imajuku Y., Ohashi Y., Aoyama T., Goto K., Oka A.
    Plant Mol. Biol. 46:205-213(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "A plant-specific cyclin-dependent kinase is involved in the control of G2/M progression in plants."
    Porceddu A., Stals H., Reichheld J.-P., Segers G., de Veylder L., Barroco R.M., Casteels P., van Montagu M., Inze D., Mironov V.
    J. Biol. Chem. 276:36354-36360(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, MUTAGENESIS OF ASP-161.
  13. "CKS1At overexpression in Arabidopsis thaliana inhibits growth by reducing meristem size and inhibiting cell-cycle progression."
    de Veylder L., Beemster G.T.S., Beeckman T., Inze D.
    Plant J. 25:617-626(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CKS1.
  14. "Genome-wide analysis of core cell cycle genes in Arabidopsis."
    Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D.
    Plant Cell 14:903-916(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  15. "Molecular characterization of Arabidopsis PHO80-like proteins, a novel class of CDKA;1-interacting cyclins."
    Torres Acosta J.A., de Almeida Engler J., Raes J., Magyar Z., de Groodt R., Inze D., de Veylder L.
    Cell. Mol. Life Sci. 61:1485-1497(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CYCU3-1.
  16. "B1-type cyclin-dependent kinases are essential for the formation of stomatal complexes in Arabidopsis thaliana."
    Boudolf V., Barroco R.M., de Almeida Engler J., Verkest A., Beeckman T., Naudts M., Inze D., de Veylder L.
    Plant Cell 16:945-955(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF ASP-161.
  17. "The plant-specific cyclin-dependent kinase CDKB1;1 and transcription factor E2Fa-DPa control the balance of mitotically dividing and endoreduplicating cells in Arabidopsis."
    Boudolf V., Vlieghe K., Beemster G.T.S., Magyar Z., Torres Acosta J.A., Maes S., Van Der Schueren E., Inze D., De Veylder L.
    Plant Cell 16:2683-2692(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  18. "Cell cycle regulation in plant development."
    Inze D., de Veylder L.
    Annu. Rev. Genet. 40:77-105(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiCKB11_ARATH
AccessioniPrimary (citable) accession number: P25859
Secondary accession number(s): Q147L4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: November 1, 1995
Last modified: September 3, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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