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P25859

- CKB11_ARATH

UniProt

P25859 - CKB11_ARATH

Protein

Cyclin-dependent kinase B1-1

Gene

CDKB1-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 2 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    May control G2/M (mitosis) phase progression. Plays a role in regulating seedling growth in darkness via regulation of hypocotyl cell elongation and cotyledon cell development. Plays a role in stomatal development. Required to suppress endoreduplication.5 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

    Enzyme regulationi

    Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-176 activates it.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331ATPPROSITE-ProRule annotation
    Active sitei142 – 1421Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: TAIR
    3. protein binding Source: IntAct
    4. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cotyledon development Source: TAIR
    2. DNA endoreduplication Source: TAIR
    3. mitotic nuclear division Source: UniProtKB-KW
    4. regulation of cell cycle Source: GOC
    5. stomatal complex formation Source: TAIR
    6. unidimensional cell growth Source: TAIR

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT3G54180-MONOMER.
    BRENDAi2.7.11.22. 399.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase B1-1 (EC:2.7.11.22, EC:2.7.11.23)
    Short name:
    CDKB1;1
    Alternative name(s):
    Cell division control protein 2 homolog B
    Gene namesi
    Name:CDKB1-1
    Synonyms:CDC2B
    Ordered Locus Names:At3g54180
    ORF Names:F24B22.140
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G54180.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: TAIR
    2. nucleus Source: TAIR

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi14 – 141T → A: Decreased kinase activity and disturbed cell cycle; when associated with F-15. 1 Publication
    Mutagenesisi15 – 151Y → F: Decreased kinase activity and disturbed cell cycle; when associated with A-14. 1 Publication
    Mutagenesisi161 – 1611D → N: Dominant negative. Decreased kinase activity and disturbed cell cycle. 3 Publications
    Mutagenesisi171 – 1711P → L: Decreased kinase activity and disturbed cell cycle. 1 Publication
    Mutagenesisi181 – 1811T → I: Decreased kinase activity and disturbed cell cycle. 1 Publication
    Mutagenesisi249 – 2513Missing: Decreased kinase activity and disturbed cell cycle.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 309309Cyclin-dependent kinase B1-1PRO_0000085750Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei15 – 151PhosphotyrosineBy similarity
    Modified residuei176 – 1761Phosphothreonine; by CAKBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP25859.
    PRIDEiP25859.

    Expressioni

    Tissue specificityi

    Highly expressed in guard cells and stomatal precursor cells of cotyledons. Expressed in roots, stems, flowers and siliques.2 Publications

    Developmental stagei

    Preferentially expressed in the S and G2 phases. Expressed in actively dividing cells: root and shoot apical meristems, leaf primordia and emerging lateral root meristem. Expressed in light-grown seedlings from 1 up to 7 days after germination with a peak at 2 and 3 days.3 Publications

    Gene expression databases

    GenevestigatoriP25859.

    Interactioni

    Subunit structurei

    Interacts with CKS1 and CYCU3-1.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CKS1O232497EBI-1253311,EBI-1253127

    Protein-protein interaction databases

    BioGridi9902. 47 interactions.
    IntActiP25859. 22 interactions.
    STRINGi3702.AT3G54180.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliP25859.
    SMRiP25859. Positions 1-306.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 301298Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    InParanoidiP25859.
    KOiK07760.
    OMAiRDWHEFP.
    PhylomeDBiP25859.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P25859-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEKYEKLEKV GEGTYGKVYK AMEKGTGKLV ALKKTRLEMD EEGIPPTALR    50
    EISLLQMLST SIYVVRLLCV EHVHQPSTKS QSTKSNLYLV FEYLDTDLKK 100
    FIDSYRKGPN PKPLEPFLIQ KLMFQLCKGV AHCHSHGVLH RDLKPQNLLL 150
    VKDKELLKIA DLGLGRAFTV PLKSYTHEIV TLWYRAPEVL LGSTHYSTGV 200
    DMWSVGCIFA EMVRRQALFP GDSEFQQLLH IFRLLGTPTE QQWPGVSTLR 250
    DWHVYPKWEP QDLTLAVPSL SPQGVDLLTK MLKYNPAERI SAKTALDHPY 300
    FDSLDKSQF 309
    Length:309
    Mass (Da):35,318
    Last modified:November 1, 1995 - v2
    Checksum:i8FFCD006A9A4A75B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti226 – 2261Q → R in CAA40972. (PubMed:1937013)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10851 Genomic DNA. Translation: BAA01624.1.
    AL132957 Genomic DNA. Translation: CAB70992.1.
    CP002686 Genomic DNA. Translation: AEE79196.1.
    BT026079 mRNA. Translation: ABG48435.1.
    AY084810 mRNA. Translation: AAM61376.1.
    X57840 mRNA. Translation: CAA40972.1.
    PIRiS23096.
    RefSeqiNP_190986.1. NM_115278.2.
    UniGeneiAt.10.

    Genome annotation databases

    EnsemblPlantsiAT3G54180.1; AT3G54180.1; AT3G54180.
    GeneIDi824585.
    KEGGiath:AT3G54180.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10851 Genomic DNA. Translation: BAA01624.1 .
    AL132957 Genomic DNA. Translation: CAB70992.1 .
    CP002686 Genomic DNA. Translation: AEE79196.1 .
    BT026079 mRNA. Translation: ABG48435.1 .
    AY084810 mRNA. Translation: AAM61376.1 .
    X57840 mRNA. Translation: CAA40972.1 .
    PIRi S23096.
    RefSeqi NP_190986.1. NM_115278.2.
    UniGenei At.10.

    3D structure databases

    ProteinModelPortali P25859.
    SMRi P25859. Positions 1-306.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 9902. 47 interactions.
    IntActi P25859. 22 interactions.
    STRINGi 3702.AT3G54180.1-P.

    Proteomic databases

    PaxDbi P25859.
    PRIDEi P25859.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G54180.1 ; AT3G54180.1 ; AT3G54180 .
    GeneIDi 824585.
    KEGGi ath:AT3G54180.

    Organism-specific databases

    GeneFarmi 2951. 107.
    TAIRi AT3G54180.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    InParanoidi P25859.
    KOi K07760.
    OMAi RDWHEFP.
    PhylomeDBi P25859.

    Enzyme and pathway databases

    BioCyci ARA:AT3G54180-MONOMER.
    BRENDAi 2.7.11.22. 399.

    Miscellaneous databases

    PROi P25859.

    Gene expression databases

    Genevestigatori P25859.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Exon-intron organization of the Arabidopsis thaliana protein kinase genes CDC2a and CDC2b."
      Imajuku Y., Hirayama T., Endoh H., Oka A.
      FEBS Lett. 304:73-77(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Arabidopsis ORF clones."
      Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "Identification of two cell-cycle-controlling cdc2 gene homologs in Arabidopsis thaliana."
      Hirayama T., Imajuku Y., Anai T., Matsui M., Oka A.
      Gene 105:159-165(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 140-309.
      Strain: cv. Columbia.
    7. "The Arabidopsis cyclin-dependent kinase gene cdc2bAt is preferentially expressed during S and G2 phases of the cell cycle."
      Segers G., Gadisseur I., Bergounioux C., de Almeida Engler J., Jacqmard A., van Montagu M., Inze D.
      Plant J. 10:601-612(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    8. "The Arabidopsis Cks1At protein binds the cyclin-dependent kinases Cdc2aAt and Cdc2bAt."
      de Veylder L., Segers G., Glab N., Casteels P., van Montagu M., Inze D.
      FEBS Lett. 412:446-452(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CKS1.
    9. "Mutational analysis of two Arabidopsis thaliana cyclin-dependent kinases in fission yeast."
      Porceddu A., de Veylder L., Hayles J., van Montagu M., Inze D., Mironov V.
      FEBS Lett. 446:182-188(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF THR-14; TYR-15; ASP-161; PRO-171; THR-181 AND 249-LEU--ASP-251.
    10. "An Arabidopsis cell cycle-dependent kinase-related gene, CDC2b, plays a role in regulating seedling growth in darkness."
      Yoshizumi T., Nagata N., Shimada H., Matsui M.
      Plant Cell 11:1883-1896(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE.
    11. "An upstream region of the Arabidopsis thaliana CDKA;1 (CDC2aAt) gene directs transcription during trichome development."
      Imajuku Y., Ohashi Y., Aoyama T., Goto K., Oka A.
      Plant Mol. Biol. 46:205-213(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "A plant-specific cyclin-dependent kinase is involved in the control of G2/M progression in plants."
      Porceddu A., Stals H., Reichheld J.-P., Segers G., de Veylder L., Barroco R.M., Casteels P., van Montagu M., Inze D., Mironov V.
      J. Biol. Chem. 276:36354-36360(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE, MUTAGENESIS OF ASP-161.
    13. "CKS1At overexpression in Arabidopsis thaliana inhibits growth by reducing meristem size and inhibiting cell-cycle progression."
      de Veylder L., Beemster G.T.S., Beeckman T., Inze D.
      Plant J. 25:617-626(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CKS1.
    14. "Genome-wide analysis of core cell cycle genes in Arabidopsis."
      Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D.
      Plant Cell 14:903-916(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.
    15. "Molecular characterization of Arabidopsis PHO80-like proteins, a novel class of CDKA;1-interacting cyclins."
      Torres Acosta J.A., de Almeida Engler J., Raes J., Magyar Z., de Groodt R., Inze D., de Veylder L.
      Cell. Mol. Life Sci. 61:1485-1497(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CYCU3-1.
    16. "B1-type cyclin-dependent kinases are essential for the formation of stomatal complexes in Arabidopsis thaliana."
      Boudolf V., Barroco R.M., de Almeida Engler J., Verkest A., Beeckman T., Naudts M., Inze D., de Veylder L.
      Plant Cell 16:945-955(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF ASP-161.
    17. "The plant-specific cyclin-dependent kinase CDKB1;1 and transcription factor E2Fa-DPa control the balance of mitotically dividing and endoreduplicating cells in Arabidopsis."
      Boudolf V., Vlieghe K., Beemster G.T.S., Magyar Z., Torres Acosta J.A., Maes S., Van Der Schueren E., Inze D., De Veylder L.
      Plant Cell 16:2683-2692(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    18. "Cell cycle regulation in plant development."
      Inze D., de Veylder L.
      Annu. Rev. Genet. 40:77-105(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiCKB11_ARATH
    AccessioniPrimary (citable) accession number: P25859
    Secondary accession number(s): Q147L4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 1992
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 124 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3