Cyclin-dependent kinase B1-1 - Arabidopsis thaliana (Mouse-ear cress)

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P25859 (CKB11_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 112. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase B1-1
Short name=CDKB1;1
EC=2.7.11.22
EC=2.7.11.23

Alternative name(s):
Cell division control protein 2 homolog B

Gene names

Name:	CDKB1-1
Synonyms:	CDC2B
Ordered Locus Names:	At3g54180
ORF Names:	F24B22.140

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May control G2/M (mitosis) phase progression. Plays a role in regulating seedling growth in darkness via regulation of hypocotyl cell elongation and cotyledon cell development. Plays a role in stomatal development. Required to suppress endoreduplication. Ref.10 Ref.11 Ref.12 Ref.16 Ref.17
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Enzyme regulation	Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-176 activates it By similarity.
Subunit structure	Interacts with CKS1 and CYCU3-1. Ref.8 Ref.13 Ref.15
Tissue specificity	Highly expressed in guard cells and stomatal precursor cells of cotyledons. Expressed in roots, stems, flowers and siliques. Ref.7 Ref.16
Developmental stage	Preferentially expressed in the S and G2 phases. Expressed in actively dividing cells: root and shoot apical meristems, leaf primordia and emerging lateral root meristem. Expressed in light-grown seedlings from 1 up to 7 days after germination with a peak at 2 and 3 days. Ref.7 Ref.10 Ref.12
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA endoreduplication Traceable author statement PubMed 15863515. Source: TAIR cell division Inferred from electronic annotation. Source: UniProtKB-KW cotyledon development Inferred from expression pattern Ref.10. Source: TAIR mitosis Inferred from electronic annotation. Source: UniProtKB-KW stomatal complex formation Inferred from expression pattern Ref.16. Source: TAIR unidimensional cell growth Inferred from mutant phenotype Ref.10. Source: TAIR
Cellular_component	cytoplasm Inferred from direct assay PubMed 20018602. Source: TAIR nucleus Inferred from direct assay PubMed 20018602. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase activity Inferred from sequence or structural similarity Ref.1. Source: TAIR
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CKS1	O23249	7	EBI-1253311,EBI-1253127

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 309

309

Cyclin-dependent kinase B1-1

PRO_0000085750

Regions

Domain

4 – 301

298

Protein kinase

Nucleotide binding

10 – 18

ATP By similarity

Sites

Active site

142

Proton acceptor By similarity

Binding site

ATP By similarity

Amino acid modifications

Modified residue

Phosphothreonine By similarity

Modified residue

Phosphotyrosine By similarity

Modified residue

176

Phosphothreonine; by CAK By similarity

Experimental info

Mutagenesis

T → A: Decreased kinase activity and disturbed cell cycle; when associated with F-15. Ref.9

Mutagenesis

Y → F: Decreased kinase activity and disturbed cell cycle; when associated with A-14. Ref.9

Mutagenesis

161

D → N: Dominant negative. Decreased kinase activity and disturbed cell cycle. Ref.9 Ref.12 Ref.16

Mutagenesis

171

P → L: Decreased kinase activity and disturbed cell cycle. Ref.9

Mutagenesis

181

T → I: Decreased kinase activity and disturbed cell cycle. Ref.9

Mutagenesis

249 – 251

Missing: Decreased kinase activity and disturbed cell cycle. Ref.9

Sequence conflict

226

Q → R in CAA40972. Ref.6

Sequences

Sequence

Length

Mass (Da)

Tools

P25859 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 8FFCD006A9A4A75B
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FASTA

309

35,318

        10         20         30         40         50         60 
MEKYEKLEKV GEGTYGKVYK AMEKGTGKLV ALKKTRLEMD EEGIPPTALR EISLLQMLST 

        70         80         90        100        110        120 
SIYVVRLLCV EHVHQPSTKS QSTKSNLYLV FEYLDTDLKK FIDSYRKGPN PKPLEPFLIQ 

       130        140        150        160        170        180 
KLMFQLCKGV AHCHSHGVLH RDLKPQNLLL VKDKELLKIA DLGLGRAFTV PLKSYTHEIV 

       190        200        210        220        230        240 
TLWYRAPEVL LGSTHYSTGV DMWSVGCIFA EMVRRQALFP GDSEFQQLLH IFRLLGTPTE 

       250        260        270        280        290        300 
QQWPGVSTLR DWHVYPKWEP QDLTLAVPSL SPQGVDLLTK MLKYNPAERI SAKTALDHPY 


FDSLDKSQF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Exon-intron organization of the Arabidopsis thaliana protein kinase genes CDC2a and CDC2b." Imajuku Y., Hirayama T., Endoh H., Oka A. FEBS Lett. 304:73-77(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. Tabata S. Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Arabidopsis ORF clones." Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Identification of two cell-cycle-controlling cdc2 gene homologs in Arabidopsis thaliana." Hirayama T., Imajuku Y., Anai T., Matsui M., Oka A. Gene 105:159-165(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 140-309. Strain: cv. Columbia.
[7]	"The Arabidopsis cyclin-dependent kinase gene cdc2bAt is preferentially expressed during S and G2 phases of the cell cycle." Segers G., Gadisseur I., Bergounioux C., de Almeida Engler J., Jacqmard A., van Montagu M., Inze D. Plant J. 10:601-612(1996) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[8]	"The Arabidopsis Cks1At protein binds the cyclin-dependent kinases Cdc2aAt and Cdc2bAt." de Veylder L., Segers G., Glab N., Casteels P., van Montagu M., Inze D. FEBS Lett. 412:446-452(1997) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CKS1.
[9]	"Mutational analysis of two Arabidopsis thaliana cyclin-dependent kinases in fission yeast." Porceddu A., de Veylder L., Hayles J., van Montagu M., Inze D., Mironov V. FEBS Lett. 446:182-188(1999) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF THR-14; TYR-15; ASP-161; PRO-171; THR-181 AND 249-LEU--ASP-251.
[10]	"An Arabidopsis cell cycle-dependent kinase-related gene, CDC2b, plays a role in regulating seedling growth in darkness." Yoshizumi T., Nagata N., Shimada H., Matsui M. Plant Cell 11:1883-1896(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE.
[11]	"An upstream region of the Arabidopsis thaliana CDKA;1 (CDC2aAt) gene directs transcription during trichome development." Imajuku Y., Ohashi Y., Aoyama T., Goto K., Oka A. Plant Mol. Biol. 46:205-213(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[12]	"A plant-specific cyclin-dependent kinase is involved in the control of G2/M progression in plants." Porceddu A., Stals H., Reichheld J.-P., Segers G., de Veylder L., Barroco R.M., Casteels P., van Montagu M., Inze D., Mironov V. J. Biol. Chem. 276:36354-36360(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE, MUTAGENESIS OF ASP-161.
[13]	"CKS1At overexpression in Arabidopsis thaliana inhibits growth by reducing meristem size and inhibiting cell-cycle progression." de Veylder L., Beemster G.T.S., Beeckman T., Inze D. Plant J. 25:617-626(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CKS1.
[14]	"Genome-wide analysis of core cell cycle genes in Arabidopsis." Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D. Plant Cell 14:903-916(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[15]	"Molecular characterization of Arabidopsis PHO80-like proteins, a novel class of CDKA;1-interacting cyclins." Torres Acosta J.A., de Almeida Engler J., Raes J., Magyar Z., de Groodt R., Inze D., de Veylder L. Cell. Mol. Life Sci. 61:1485-1497(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CYCU3-1.
[16]	"B1-type cyclin-dependent kinases are essential for the formation of stomatal complexes in Arabidopsis thaliana." Boudolf V., Barroco R.M., de Almeida Engler J., Verkest A., Beeckman T., Naudts M., Inze D., de Veylder L. Plant Cell 16:945-955(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF ASP-161.
[17]	"The plant-specific cyclin-dependent kinase CDKB1;1 and transcription factor E2Fa-DPa control the balance of mitotically dividing and endoreduplicating cells in Arabidopsis." Boudolf V., Vlieghe K., Beemster G.T.S., Magyar Z., Torres Acosta J.A., Maes S., Van Der Schueren E., Inze D., De Veylder L. Plant Cell 16:2683-2692(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[18]	"Cell cycle regulation in plant development." Inze D., de Veylder L. Annu. Rev. Genet. 40:77-105(2006) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10851 Genomic DNA. Translation: BAA01624.1. AL132957 Genomic DNA. Translation: CAB70992.1. CP002686 Genomic DNA. Translation: AEE79196.1. BT026079 mRNA. Translation: ABG48435.1. AY084810 mRNA. Translation: AAM61376.1. X57840 mRNA. Translation: CAA40972.1.
IPI	IPI00544568.
PIR	S23096.
RefSeq	NP_190986.1. NM_115278.2.
UniGene	At.10.
3D structure databases
ProteinModelPortal	P25859.
SMR	P25859. Positions 1-301.
ModBase	Search...
Protein-protein interaction databases
IntAct	P25859. 22 interactions.
STRING	3702.AT3G54180.1-P.
Proteomic databases
PaxDb	P25859.
PRIDE	P25859.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G54180.1; AT3G54180.1; AT3G54180.
GeneID	824585.
KEGG	ath:AT3G54180.
Organism-specific databases
GeneFarm	2951. 107.
TAIR	At3g54180.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000233024.
InParanoid	P25859.
KO	K07760.
OMA	RDWHEFP.
PhylomeDB	P25859.
ProtClustDB	CLSN2685082.
Enzyme and pathway databases
BRENDA	2.7.11.22. 399.
Gene expression databases
Genevestigator	P25859.
GermOnline	AT3G54180. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CKB11_ARATH

Accession

Primary (citable) accession number: P25859
Secondary accession number(s): Q147L4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 112 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents