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Protein

Cyclin-dependent kinase B1-1

Gene

CDKB1-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May control G2/M (mitosis) phase progression. Plays a role in regulating seedling growth in darkness via regulation of hypocotyl cell elongation and cotyledon cell development. Plays a role in stomatal development. Required to suppress endoreduplication.5 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulationi

Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-176 activates it.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33ATPPROSITE-ProRule annotation1
Active sitei142Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • cotyledon development Source: TAIR
  • DNA endoreduplication Source: TAIR
  • mitotic nuclear division Source: UniProtKB-KW
  • stomatal complex formation Source: TAIR
  • unidimensional cell growth Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase B1-1 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDKB1;1
Alternative name(s):
Cell division control protein 2 homolog B
Gene namesi
Name:CDKB1-1
Synonyms:CDC2B
Ordered Locus Names:At3g54180
ORF Names:F24B22.140
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G54180.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • nucleus Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi14T → A: Decreased kinase activity and disturbed cell cycle; when associated with F-15. 1 Publication1
Mutagenesisi15Y → F: Decreased kinase activity and disturbed cell cycle; when associated with A-14. 1 Publication1
Mutagenesisi161D → N: Dominant negative. Decreased kinase activity and disturbed cell cycle. 3 Publications1
Mutagenesisi171P → L: Decreased kinase activity and disturbed cell cycle. 1 Publication1
Mutagenesisi181T → I: Decreased kinase activity and disturbed cell cycle. 1 Publication1
Mutagenesisi249 – 251Missing : Decreased kinase activity and disturbed cell cycle. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000857501 – 309Cyclin-dependent kinase B1-1Add BLAST309

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphotyrosineBy similarity1
Modified residuei176Phosphothreonine; by CAKBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP25859.
PRIDEiP25859.

PTM databases

iPTMnetiP25859.

Expressioni

Tissue specificityi

Highly expressed in guard cells and stomatal precursor cells of cotyledons. Expressed in roots, stems, flowers and siliques.2 Publications

Developmental stagei

Preferentially expressed in the S and G2 phases. Expressed in actively dividing cells: root and shoot apical meristems, leaf primordia and emerging lateral root meristem. Expressed in light-grown seedlings from 1 up to 7 days after germination with a peak at 2 and 3 days.3 Publications

Gene expression databases

GenevisibleiP25859. AT.

Interactioni

Subunit structurei

Interacts with CKS1 (PubMed:9276444, PubMed:11319029). Interacts with CYCU3-1 (PubMed:15197472). Interacts with SIM, SMR1 and SMR2 (PubMed:20706207).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CKS1O232497EBI-1253311,EBI-1253127

Protein-protein interaction databases

BioGridi9902. 50 interactors.
IntActiP25859. 22 interactors.
STRINGi3702.AT3G54180.1.

Structurei

3D structure databases

ProteinModelPortaliP25859.
SMRiP25859.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 301Protein kinasePROSITE-ProRule annotationAdd BLAST298

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0594. Eukaryota.
ENOG410XPP3. LUCA.
HOGENOMiHOG000233024.
InParanoidiP25859.
KOiK07760.
OMAiMLSTSIY.
OrthoDBiEOG09360F64.
PhylomeDBiP25859.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25859-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKYEKLEKV GEGTYGKVYK AMEKGTGKLV ALKKTRLEMD EEGIPPTALR
60 70 80 90 100
EISLLQMLST SIYVVRLLCV EHVHQPSTKS QSTKSNLYLV FEYLDTDLKK
110 120 130 140 150
FIDSYRKGPN PKPLEPFLIQ KLMFQLCKGV AHCHSHGVLH RDLKPQNLLL
160 170 180 190 200
VKDKELLKIA DLGLGRAFTV PLKSYTHEIV TLWYRAPEVL LGSTHYSTGV
210 220 230 240 250
DMWSVGCIFA EMVRRQALFP GDSEFQQLLH IFRLLGTPTE QQWPGVSTLR
260 270 280 290 300
DWHVYPKWEP QDLTLAVPSL SPQGVDLLTK MLKYNPAERI SAKTALDHPY

FDSLDKSQF
Length:309
Mass (Da):35,318
Last modified:November 1, 1995 - v2
Checksum:i8FFCD006A9A4A75B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti226Q → R in CAA40972 (PubMed:1937013).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10851 Genomic DNA. Translation: BAA01624.1.
AL132957 Genomic DNA. Translation: CAB70992.1.
CP002686 Genomic DNA. Translation: AEE79196.1.
BT026079 mRNA. Translation: ABG48435.1.
AY084810 mRNA. Translation: AAM61376.1.
X57840 mRNA. Translation: CAA40972.1.
PIRiS23096.
RefSeqiNP_190986.1. NM_115278.3.
UniGeneiAt.10.

Genome annotation databases

EnsemblPlantsiAT3G54180.1; AT3G54180.1; AT3G54180.
GeneIDi824585.
GrameneiAT3G54180.1; AT3G54180.1; AT3G54180.
KEGGiath:AT3G54180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10851 Genomic DNA. Translation: BAA01624.1.
AL132957 Genomic DNA. Translation: CAB70992.1.
CP002686 Genomic DNA. Translation: AEE79196.1.
BT026079 mRNA. Translation: ABG48435.1.
AY084810 mRNA. Translation: AAM61376.1.
X57840 mRNA. Translation: CAA40972.1.
PIRiS23096.
RefSeqiNP_190986.1. NM_115278.3.
UniGeneiAt.10.

3D structure databases

ProteinModelPortaliP25859.
SMRiP25859.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9902. 50 interactors.
IntActiP25859. 22 interactors.
STRINGi3702.AT3G54180.1.

PTM databases

iPTMnetiP25859.

Proteomic databases

PaxDbiP25859.
PRIDEiP25859.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G54180.1; AT3G54180.1; AT3G54180.
GeneIDi824585.
GrameneiAT3G54180.1; AT3G54180.1; AT3G54180.
KEGGiath:AT3G54180.

Organism-specific databases

TAIRiAT3G54180.

Phylogenomic databases

eggNOGiKOG0594. Eukaryota.
ENOG410XPP3. LUCA.
HOGENOMiHOG000233024.
InParanoidiP25859.
KOiK07760.
OMAiMLSTSIY.
OrthoDBiEOG09360F64.
PhylomeDBiP25859.

Enzyme and pathway databases

BRENDAi2.7.11.22. 399.

Miscellaneous databases

PROiP25859.

Gene expression databases

GenevisibleiP25859. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCKB11_ARATH
AccessioniPrimary (citable) accession number: P25859
Secondary accession number(s): Q147L4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.