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P25859 (CKB11_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-dependent kinase B1-1

Short name=CDKB1;1
EC=2.7.11.22
EC=2.7.11.23
Alternative name(s):
Cell division control protein 2 homolog B
Gene names
Name:CDKB1-1
Synonyms:CDC2B
Ordered Locus Names:At3g54180
ORF Names:F24B22.140
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May control G2/M (mitosis) phase progression. Plays a role in regulating seedling growth in darkness via regulation of hypocotyl cell elongation and cotyledon cell development. Plays a role in stomatal development. Required to suppress endoreduplication. Ref.10 Ref.11 Ref.12 Ref.16 Ref.17

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulation

Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-176 activates it By similarity.

Subunit structure

Interacts with CKS1 and CYCU3-1. Ref.8 Ref.13 Ref.15

Tissue specificity

Highly expressed in guard cells and stomatal precursor cells of cotyledons. Expressed in roots, stems, flowers and siliques. Ref.7 Ref.16

Developmental stage

Preferentially expressed in the S and G2 phases. Expressed in actively dividing cells: root and shoot apical meristems, leaf primordia and emerging lateral root meristem. Expressed in light-grown seedlings from 1 up to 7 days after germination with a peak at 2 and 3 days. Ref.7 Ref.10 Ref.12

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CKS1O232497EBI-1253311,EBI-1253127

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 309309Cyclin-dependent kinase B1-1
PRO_0000085750

Regions

Domain4 – 301298Protein kinase
Nucleotide binding10 – 189ATP By similarity

Sites

Active site1421Proton acceptor By similarity
Binding site331ATP By similarity

Amino acid modifications

Modified residue151Phosphotyrosine By similarity
Modified residue1761Phosphothreonine; by CAK By similarity

Experimental info

Mutagenesis141T → A: Decreased kinase activity and disturbed cell cycle; when associated with F-15. Ref.9
Mutagenesis151Y → F: Decreased kinase activity and disturbed cell cycle; when associated with A-14. Ref.9
Mutagenesis1611D → N: Dominant negative. Decreased kinase activity and disturbed cell cycle. Ref.9 Ref.12 Ref.16
Mutagenesis1711P → L: Decreased kinase activity and disturbed cell cycle. Ref.9
Mutagenesis1811T → I: Decreased kinase activity and disturbed cell cycle. Ref.9
Mutagenesis249 – 2513Missing: Decreased kinase activity and disturbed cell cycle. Ref.9
Sequence conflict2261Q → R in CAA40972. Ref.6

Sequences

Sequence LengthMass (Da)Tools
P25859 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 8FFCD006A9A4A75B

FASTA30935,318
        10         20         30         40         50         60 
MEKYEKLEKV GEGTYGKVYK AMEKGTGKLV ALKKTRLEMD EEGIPPTALR EISLLQMLST 

        70         80         90        100        110        120 
SIYVVRLLCV EHVHQPSTKS QSTKSNLYLV FEYLDTDLKK FIDSYRKGPN PKPLEPFLIQ 

       130        140        150        160        170        180 
KLMFQLCKGV AHCHSHGVLH RDLKPQNLLL VKDKELLKIA DLGLGRAFTV PLKSYTHEIV 

       190        200        210        220        230        240 
TLWYRAPEVL LGSTHYSTGV DMWSVGCIFA EMVRRQALFP GDSEFQQLLH IFRLLGTPTE 

       250        260        270        280        290        300 
QQWPGVSTLR DWHVYPKWEP QDLTLAVPSL SPQGVDLLTK MLKYNPAERI SAKTALDHPY 


FDSLDKSQF 

« Hide

References

« Hide 'large scale' references
[1]"Exon-intron organization of the Arabidopsis thaliana protein kinase genes CDC2a and CDC2b."
Imajuku Y., Hirayama T., Endoh H., Oka A.
FEBS Lett. 304:73-77(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"Identification of two cell-cycle-controlling cdc2 gene homologs in Arabidopsis thaliana."
Hirayama T., Imajuku Y., Anai T., Matsui M., Oka A.
Gene 105:159-165(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 140-309.
Strain: cv. Columbia.
[7]"The Arabidopsis cyclin-dependent kinase gene cdc2bAt is preferentially expressed during S and G2 phases of the cell cycle."
Segers G., Gadisseur I., Bergounioux C., de Almeida Engler J., Jacqmard A., van Montagu M., Inze D.
Plant J. 10:601-612(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[8]"The Arabidopsis Cks1At protein binds the cyclin-dependent kinases Cdc2aAt and Cdc2bAt."
de Veylder L., Segers G., Glab N., Casteels P., van Montagu M., Inze D.
FEBS Lett. 412:446-452(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CKS1.
[9]"Mutational analysis of two Arabidopsis thaliana cyclin-dependent kinases in fission yeast."
Porceddu A., de Veylder L., Hayles J., van Montagu M., Inze D., Mironov V.
FEBS Lett. 446:182-188(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF THR-14; TYR-15; ASP-161; PRO-171; THR-181 AND 249-LEU--ASP-251.
[10]"An Arabidopsis cell cycle-dependent kinase-related gene, CDC2b, plays a role in regulating seedling growth in darkness."
Yoshizumi T., Nagata N., Shimada H., Matsui M.
Plant Cell 11:1883-1896(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE.
[11]"An upstream region of the Arabidopsis thaliana CDKA;1 (CDC2aAt) gene directs transcription during trichome development."
Imajuku Y., Ohashi Y., Aoyama T., Goto K., Oka A.
Plant Mol. Biol. 46:205-213(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"A plant-specific cyclin-dependent kinase is involved in the control of G2/M progression in plants."
Porceddu A., Stals H., Reichheld J.-P., Segers G., de Veylder L., Barroco R.M., Casteels P., van Montagu M., Inze D., Mironov V.
J. Biol. Chem. 276:36354-36360(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE, MUTAGENESIS OF ASP-161.
[13]"CKS1At overexpression in Arabidopsis thaliana inhibits growth by reducing meristem size and inhibiting cell-cycle progression."
de Veylder L., Beemster G.T.S., Beeckman T., Inze D.
Plant J. 25:617-626(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CKS1.
[14]"Genome-wide analysis of core cell cycle genes in Arabidopsis."
Vandepoele K., Raes J., de Veylder L., Rouze P., Rombauts S., Inze D.
Plant Cell 14:903-916(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[15]"Molecular characterization of Arabidopsis PHO80-like proteins, a novel class of CDKA;1-interacting cyclins."
Torres Acosta J.A., de Almeida Engler J., Raes J., Magyar Z., de Groodt R., Inze D., de Veylder L.
Cell. Mol. Life Sci. 61:1485-1497(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CYCU3-1.
[16]"B1-type cyclin-dependent kinases are essential for the formation of stomatal complexes in Arabidopsis thaliana."
Boudolf V., Barroco R.M., de Almeida Engler J., Verkest A., Beeckman T., Naudts M., Inze D., de Veylder L.
Plant Cell 16:945-955(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF ASP-161.
[17]"The plant-specific cyclin-dependent kinase CDKB1;1 and transcription factor E2Fa-DPa control the balance of mitotically dividing and endoreduplicating cells in Arabidopsis."
Boudolf V., Vlieghe K., Beemster G.T.S., Magyar Z., Torres Acosta J.A., Maes S., Van Der Schueren E., Inze D., De Veylder L.
Plant Cell 16:2683-2692(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[18]"Cell cycle regulation in plant development."
Inze D., de Veylder L.
Annu. Rev. Genet. 40:77-105(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D10851 Genomic DNA. Translation: BAA01624.1.
AL132957 Genomic DNA. Translation: CAB70992.1.
CP002686 Genomic DNA. Translation: AEE79196.1.
BT026079 mRNA. Translation: ABG48435.1.
AY084810 mRNA. Translation: AAM61376.1.
X57840 mRNA. Translation: CAA40972.1.
PIRS23096.
RefSeqNP_190986.1. NM_115278.2.
UniGeneAt.10.

3D structure databases

ProteinModelPortalP25859.
SMRP25859. Positions 1-306.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid9902. 47 interactions.
IntActP25859. 22 interactions.
STRING3702.AT3G54180.1-P.

Proteomic databases

PaxDbP25859.
PRIDEP25859.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G54180.1; AT3G54180.1; AT3G54180.
GeneID824585.
KEGGath:AT3G54180.

Organism-specific databases

GeneFarm2951. 107.
TAIRAT3G54180.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
InParanoidP25859.
KOK07760.
OMARDWHEFP.
PhylomeDBP25859.

Enzyme and pathway databases

BioCycARA:AT3G54180-MONOMER.
BRENDA2.7.11.22. 399.

Gene expression databases

GenevestigatorP25859.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP25859.

Entry information

Entry nameCKB11_ARATH
AccessionPrimary (citable) accession number: P25859
Secondary accession number(s): Q147L4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names