Reviewed, UniProtKB/Swiss-Prot P25858 (G3PC_ARATH)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase, cytosolic
    EC=1.2.1.12

Gene names

Name:	GAPC
Ordered Locus Names:	At3g04120
ORF Names:	T6K12.26

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Miscellaneous	Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Traceable author statement. Source: TAIR glycolysis Inferred from direct assay. Source: TAIR oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to cadmium ion Inferred from expression pattern. Source: TAIR response to heat Inferred from expression pattern. Source: TAIR response to hydrogen peroxide Inferred from direct assay. Source: TAIR response to salt stress Inferred from expression pattern. Source: TAIR response to sucrose stimulus Inferred from expression pattern. Source: TAIR seed development Inferred from mutant phenotype. Source: TAIR
Cellular component	apoplast Inferred from direct assay. Source: TAIR chloroplast Inferred from direct assay. Source: TAIR cytosol Traceable author statement. Source: TAIR mitochondrial envelope Inferred from direct assay. Source: TAIR nucleus Inferred from direct assay. Source: TAIR plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Traceable author statement. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Glyceraldehyde-3-phosphate dehydrogenase, cytosolic		PRO_0000145594
Regions
	Nucleotide binding	15 – 16	2	NAD By similarity
	Region	155 – 157	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	215 – 216	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	156	1	Nucleophile By similarity
	Binding site	37	1	NAD By similarity
	Binding site	84	1	NAD; via carbonyl oxygen By similarity
	Binding site	186	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	238	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	320	1	NAD By similarity
	Site	183	1	Activates thiol group during catalysis By similarity
Experimental info
	Sequence conflict	127	1	A → E in AAA32794. Ref.1
	Sequence conflict	127	1	A → E in AAA32796. Ref.1
	Sequence conflict	260	1	D → E in AAA32794. Ref.1
	Sequence conflict	260	1	D → E in AAA32796. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P25858-1 [UniParc]. Last modified May 2, 2002. Version 2. Checksum: 4186F65E1F1EE96F	FASTA	338	36,914
	10 20 30 40 50 60 MADKKIRIGI NGFGRIGRLV ARVVLQRDDV ELVAVNDPFI TTEYMTYMFK YDSVHGQWKH 70 80 90 100 110 120 NELKIKDEKT LLFGEKPVTV FGIRNPEDIP WAEAGADYVV ESTGVFTDKD KAAAHLKGGA 130 140 150 160 170 180 KKVVISAPSK DAPMFVVGVN EHEYKSDLDI VSNASCTTNC LAPLAKVIND RFGIVEGLMT 190 200 210 220 230 240 TVHSITATQK TVDGPSMKDW RGGRAASFNI IPSSTGAAKA VGKVLPALNG KLTGMSFRVP 250 260 270 280 290 300 TVDVSVVDLT VRLEKAATYD EIKKAIKEES EGKLKGILGY TEDDVVSTDF VGDNRSSIFD 310 320 330 AKAGIALSDK FVKLVSWYDN EWGYSSRVVD LIVHMSKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and chromosomal mapping of nuclear genes encoding chloroplast and cytosolic glyceraldehyde-3-phosphate-dehydrogenase from Arabidopsis thaliana." Shih M.-C., Heinrich P., Goodman H.M. Gene 104:133-138(1991) [PubMed: 1916285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	Erratum Shih M.-C., Heinrich P., Goodman H.M. Gene 119:317-319(1992) [PubMed: 1398114] [Abstract]
[3]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non-redundant ESTs." Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C., Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R., Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J. , Fleck J., Clement B., Philipps G., Herve C., Bardet C., Tremousaygue D., Lescure B., Lacomme C., Roby D., Jourjon M.-F., Chabrier P., Charpenteau J.-L., Desprez T., Amselem J., Chiapello H., Hoefte H. Plant J. 9:101-124(1996) [PubMed: 8580968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 181-321. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M64116 mRNA. Translation: AAA32794.1. M64119 Genomic DNA. Translation: AAA32796.1. AC016829 Genomic DNA. Translation: AAF26801.1. AY052267 mRNA. Translation: AAK97737.1. AY060521 mRNA. Translation: AAL31134.1. AY140084 mRNA. Translation: AAM98225.1. F20074 mRNA. Translation: CAA23391.1.
IPI	IPI00527494.
PIR	JQ1287.
RefSeq	NP_187062.1.
UniGene	At.22963 At.24406 At.71328 Rra.2758 Rsa.1405 Rsa.25743
3D structure databases
SMR	P25858. Positions 7-337.
ModBase	Search...
Protein-protein interaction databases
STRING	P25858.
Proteomic databases
PRIDE	P25858.
Genome annotation databases
GeneID	819567.
GenomeReviews	Gene locus AT3G04120 in contig BA000014_GR.
KEGG	ath:AT3G04120.
Organism-specific databases
TAIR	At3g04120.
Phylogenomic databases
eggNOG	KOG0657.
HOGENOM	HBG571736.
InParanoid	P25858.
OMA	DADACIS.
PhylomeDB	P25858.
Enzyme and pathway databases
BRENDA	1.2.1.12. 302.
Gene expression databases
ArrayExpress	P25858.
Genevestigator	P25858.
GermOnline	AT3G04120. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR020830. GlycerAld_3-P_DH_AS. IPR020829. GlycerAld_3-P_DH_cat. IPR020832. GlycerAld_3-P_DH_cat_sub. IPR020831. GlycerAld_3-P_DH_family. IPR020828. GlycerAld_3-P_DH_NAD(P)_bd. IPR000173. GlycerAld_3-P_DH_subfam. IPR006424. Glyceraldehyde-3-P_DH_1. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
SMART	SM00846. Gp_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3PC_ARATH

Accession

Primary (citable) accession number: P25858
Secondary accession number(s): Q42352, Q9M8W8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 2, 2002
Last modified:	February 9, 2010
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)