Skip Header

Contribute Send feedback
Read comments (?) or add your own

P25857 (G3PB_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic
EC=1.2.1.13
Alternative name(s):
NADP-dependent glyceraldehydephosphate dehydrogenase subunit B
Gene names
Name:GAPB
Ordered Locus Names:At1g42970
ORF Names:F13A11.3
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length447 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

D-glyceraldehyde 3-phosphate + phosphate + NADP+ = 3-phospho-D-glyceroyl phosphate + NADPH.

Pathway

Carbohydrate biosynthesis; Calvin cycle.

Subunit structure

Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1) By similarity.

Subcellular location

Plastid › chloroplast membrane; Peripheral membrane protein. Plastid › chloroplast stroma Ref.6.

Miscellaneous

Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes.

Sequence similarities

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 8080Chloroplast By similarity
Chain81 – 447367Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic
PRO_0000010417

Regions

Nucleotide binding91 – 922NADP By similarity
Region234 – 2363Glyceraldehyde 3-phosphate binding By similarity
Region293 – 2942Glyceraldehyde 3-phosphate binding By similarity

Sites

Active site2351Nucleophile By similarity
Binding site1151NADP By similarity
Binding site1601NADP; via carbonyl oxygen By similarity
Binding site2651Glyceraldehyde 3-phosphate By similarity
Binding site2801Glyceraldehyde 3-phosphate By similarity
Binding site3161Glyceraldehyde 3-phosphate By similarity
Binding site3991NADP By similarity
Site2621Activates thiol group during catalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
P25857 [UniParc].

Last modified May 2, 2002. Version 2.
Checksum: E7DBD59915560EF3

FASTA44747,660
        10         20         30         40         50         60 
MATHAALAVS RIPVTQRLQS KSAIHSFPAQ CSSKRLEVAE FSGLRMSSIG GEASFFDAVA 

        70         80         90        100        110        120 
AQIIPKAVTT STPVRGETVA KLKVAINGFG RIGRNFLRCW HGRKDSPLEV VVLNDSGGVK 

       130        140        150        160        170        180 
NASHLLKYDS MLGTFKAEVK IVDNETISVD GKLIKVVSNR DPLKLPWAEL GIDIVIEGTG 

       190        200        210        220        230        240 
VFVDGPGAGK HIQAGASKVI ITAPAKGADI PTYVMGVNEQ DYGHDVANII SNASCTTNCL 

       250        260        270        280        290        300 
APFAKVLDEE FGIVKGTMTT THSYTGDQRL LDASHRDLRR ARAAALNIVP TSTGAAKAVS 

       310        320        330        340        350        360 
LVLPQLKGKL NGIALRVPTP NVSVVDLVIN VEKKGLTAED VNEAFRKAAN GPMKGILDVC 

       370        380        390        400        410        420 
DAPLVSVDFR CSDVSTTIDS SLTMVMGDDM VKVVAWYDNE WGYSQRVVDL AHLVASKWPG 

       430        440 
AEAVGSGDPL EDFCKTNPAD EECKVYD

« Hide

References

« Hide 'large scale' references
[1]"Cloning and chromosomal mapping of nuclear genes encoding chloroplast and cytosolic glyceraldehyde-3-phosphate-dehydrogenase from Arabidopsis thaliana."
Shih M.-C., Heinrich P., Goodman H.M.
Gene 104:133-138(1991) [PubMed: 1916285] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Tissue: Leaf.
[2]Erratum
Shih M.-C., Heinrich P., Goodman H.M.
Gene 119:317-319(1992) [PubMed: 1398114] [Abstract]
[3]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana."
Ferro M., Salvi D., Brugiere S., Miras S., Kowalski S., Louwagie M., Garin J., Joyard J., Rolland N.
Mol. Cell. Proteomics 2:325-345(2003) [PubMed: 12766230] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
Strain: cv. Wassilewskija.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M64115 mRNA. Translation: AAD10210.1.
M64118 Genomic DNA. Translation: AAA32795.1.
AC068324 Genomic DNA. Translation: AAG51517.1.
CP002684 Genomic DNA. Translation: AEE31933.1.
AY039961 mRNA. Translation: AAK64065.1.
AY039539 mRNA. Translation: AAK62594.1.
AY079402 mRNA. Translation: AAL85133.1.
AY095991 mRNA. Translation: AAM19948.1.
AY140091 mRNA. Translation: AAM98232.1.
BT002267 mRNA. Translation: AAN72278.1.
IPIIPI00541680.
PIRC96497.
JQ1286.
RefSeqNP_174996.1. NM_103456.3.
UniGeneAt.21749.
At.67261.

3D structure databases

ProteinModelPortalP25857.
SMRP25857. Positions 81-422.
ModBaseSearch...

Protein-protein interaction databases

IntActP25857. 2 interactions.
STRINGP25857.

2D gel databases

SWISS-2DPAGEP25857.

Proteomic databases

PRIDEP25857.
ProMEXP25857.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G42970.1; AT1G42970.1; AT1G42970.
GeneID840895.
GenomeReviewsGene locus AT1G42970 in contig CT485782_GR.
KEGGath:AT1G42970.

Organism-specific databases

TAIRAt1g42970.

Phylogenomic databases

eggNOGKOG0657.
HOGENOMHBG571736.
InParanoidP25857.
OMAIGRNAFK.
PhylomeDBP25857.
ProtClustDBPLN02237.

Enzyme and pathway databases

BRENDA1.2.1.13. 399.

Gene expression databases

ArrayExpressP25857.
GenevestigatorP25857.
GermOnlineAT1G42970. Arabidopsis thaliana.

Family and domain databases

InterProIPR003823. DUF_CP12.
IPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK05298.
PANTHERPTHR10836. GAP_DH. 1 hit.
PfamPF02672. CP12. 1 hit.
PF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PRINTSPR00078. G3PDHDRGNASE.
SMARTSM00846. Gp_dh_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01534. GAPDH-I. 1 hit.
PROSITEPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG3PB_ARATH
AccessionPrimary (citable) accession number: P25857
Secondary accession number(s): Q9C7S2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 2, 2002
Last modified: January 25, 2012
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents