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Protein

Glyceraldehyde-3-phosphate dehydrogenase GAPB, chloroplastic

Gene

GAPB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the photosynthetic reductive pentose phosphate pathway (Calvin-Benson cycle). Catalyzes the reduction of 1,3-diphosphoglycerate by NADPH (By similarity).By similarity

Miscellaneous

Plants contain three types of GAPDH: NAD-dependent cytosolic forms which participate in glycolysis, NAD-dependent chloroplastic forms which participate in plastidic glycolysis and NADP-dependent chloroplastic forms which participate in the photosynthetic reductive pentose phosphate pathway (Calvin-Benson cycle). All the forms are encoded by distinct genes.

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NADP+ = 3-phospho-D-glyceroyl phosphate + NADPH.

Pathwayi: Calvin cycle

This protein is involved in the pathway Calvin cycle, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway Calvin cycle and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei115NADPBy similarity1
Binding sitei160NADP; via carbonyl oxygenBy similarity1
Active sitei235NucleophilePROSITE-ProRule annotation1
Sitei262Activates thiol group during catalysisBy similarity1
Binding sitei265Glyceraldehyde 3-phosphateBy similarity1
Binding sitei280Glyceraldehyde 3-phosphateBy similarity1
Binding sitei316Glyceraldehyde 3-phosphateBy similarity1
Binding sitei399NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi91 – 92NADPBy similarity2

GO - Molecular functioni

GO - Biological processi

  • glucose metabolic process Source: InterPro
  • reductive pentose-phosphate cycle Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to cold Source: TAIR
  • response to light stimulus Source: TAIR
  • response to sucrose Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processCalvin cycle
LigandNADP

Enzyme and pathway databases

BRENDAi1.2.1.13. 399.
UniPathwayiUPA00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase GAPB, chloroplastic (EC:1.2.1.13)
Alternative name(s):
NADP-dependent glyceraldehydephosphate dehydrogenase B
Gene namesi
Name:GAPB
Ordered Locus Names:At1g42970
ORF Names:F13A11.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRilocus:2009864. AT1G42970.

Subcellular locationi

  • Plastidchloroplast membrane By similarity; Peripheral membrane protein By similarity
  • Plastidchloroplast stroma By similarity

GO - Cellular componenti

  • apoplast Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast membrane Source: UniProtKB-SubCell
  • chloroplast stroma Source: TAIR
  • chloroplast thylakoid membrane Source: TAIR
  • membrane Source: TAIR
  • stromule Source: TAIR

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 80ChloroplastBy similarityAdd BLAST80
ChainiPRO_000001041781 – 447Glyceraldehyde-3-phosphate dehydrogenase GAPB, chloroplasticAdd BLAST367

Proteomic databases

PaxDbiP25857.
PRIDEiP25857.

2D gel databases

SWISS-2DPAGEiP25857.

PTM databases

iPTMnetiP25857.

Expressioni

Tissue specificityi

Expressed in leaves and stems.1 Publication

Inductioni

Repressed by darkness and sucrose.1 Publication

Gene expression databases

GenevisibleiP25857. AT.

Interactioni

Subunit structurei

Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1).By similarity

Protein-protein interaction databases

BioGridi26120. 7 interactors.
IntActiP25857. 2 interactors.
STRINGi3702.AT1G42970.1.

Structurei

3D structure databases

ProteinModelPortaliP25857.
SMRiP25857.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni234 – 236Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni293 – 294Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
HOGENOMiHOG000071678.
InParanoidiP25857.
KOiK05298.
OMAiCSDVSTT.
OrthoDBiEOG09360CQC.
PhylomeDBiP25857.

Family and domain databases

InterProiView protein in InterPro
IPR003823. DUF_CP12.
IPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
PfamiView protein in Pfam
PF02672. CP12. 1 hit.
PF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiView protein in SMART
SM00846. Gp_dh_N. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiView protein in PROSITE
PS00071. GAPDH. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25857-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATHAALAVS RIPVTQRLQS KSAIHSFPAQ CSSKRLEVAE FSGLRMSSIG
60 70 80 90 100
GEASFFDAVA AQIIPKAVTT STPVRGETVA KLKVAINGFG RIGRNFLRCW
110 120 130 140 150
HGRKDSPLEV VVLNDSGGVK NASHLLKYDS MLGTFKAEVK IVDNETISVD
160 170 180 190 200
GKLIKVVSNR DPLKLPWAEL GIDIVIEGTG VFVDGPGAGK HIQAGASKVI
210 220 230 240 250
ITAPAKGADI PTYVMGVNEQ DYGHDVANII SNASCTTNCL APFAKVLDEE
260 270 280 290 300
FGIVKGTMTT THSYTGDQRL LDASHRDLRR ARAAALNIVP TSTGAAKAVS
310 320 330 340 350
LVLPQLKGKL NGIALRVPTP NVSVVDLVIN VEKKGLTAED VNEAFRKAAN
360 370 380 390 400
GPMKGILDVC DAPLVSVDFR CSDVSTTIDS SLTMVMGDDM VKVVAWYDNE
410 420 430 440
WGYSQRVVDL AHLVASKWPG AEAVGSGDPL EDFCKTNPAD EECKVYD
Length:447
Mass (Da):47,660
Last modified:May 2, 2002 - v2
Checksum:iE7DBD59915560EF3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64115 mRNA. Translation: AAD10210.1.
M64118 Genomic DNA. Translation: AAA32795.1.
AC068324 Genomic DNA. Translation: AAG51517.1.
CP002684 Genomic DNA. Translation: AEE31933.1.
AY039961 mRNA. Translation: AAK64065.1.
AY039539 mRNA. Translation: AAK62594.1.
AY079402 mRNA. Translation: AAL85133.1.
AY095991 mRNA. Translation: AAM19948.1.
AY140091 mRNA. Translation: AAM98232.1.
BT002267 mRNA. Translation: AAN72278.1.
AK230314 mRNA. Translation: BAF02115.1.
PIRiC96497.
JQ1286.
RefSeqiNP_174996.1. NM_103456.4.
UniGeneiAt.21749.
At.67261.

Genome annotation databases

EnsemblPlantsiAT1G42970.1; AT1G42970.1; AT1G42970.
GeneIDi840895.
GrameneiAT1G42970.1; AT1G42970.1; AT1G42970.
KEGGiath:AT1G42970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64115 mRNA. Translation: AAD10210.1.
M64118 Genomic DNA. Translation: AAA32795.1.
AC068324 Genomic DNA. Translation: AAG51517.1.
CP002684 Genomic DNA. Translation: AEE31933.1.
AY039961 mRNA. Translation: AAK64065.1.
AY039539 mRNA. Translation: AAK62594.1.
AY079402 mRNA. Translation: AAL85133.1.
AY095991 mRNA. Translation: AAM19948.1.
AY140091 mRNA. Translation: AAM98232.1.
BT002267 mRNA. Translation: AAN72278.1.
AK230314 mRNA. Translation: BAF02115.1.
PIRiC96497.
JQ1286.
RefSeqiNP_174996.1. NM_103456.4.
UniGeneiAt.21749.
At.67261.

3D structure databases

ProteinModelPortaliP25857.
SMRiP25857.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi26120. 7 interactors.
IntActiP25857. 2 interactors.
STRINGi3702.AT1G42970.1.

PTM databases

iPTMnetiP25857.

2D gel databases

SWISS-2DPAGEiP25857.

Proteomic databases

PaxDbiP25857.
PRIDEiP25857.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G42970.1; AT1G42970.1; AT1G42970.
GeneIDi840895.
GrameneiAT1G42970.1; AT1G42970.1; AT1G42970.
KEGGiath:AT1G42970.

Organism-specific databases

AraportiAT1G42970.
TAIRilocus:2009864. AT1G42970.

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
HOGENOMiHOG000071678.
InParanoidiP25857.
KOiK05298.
OMAiCSDVSTT.
OrthoDBiEOG09360CQC.
PhylomeDBiP25857.

Enzyme and pathway databases

UniPathwayiUPA00116.
BRENDAi1.2.1.13. 399.

Miscellaneous databases

PROiPR:P25857.

Gene expression databases

GenevisibleiP25857. AT.

Family and domain databases

InterProiView protein in InterPro
IPR003823. DUF_CP12.
IPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
PfamiView protein in Pfam
PF02672. CP12. 1 hit.
PF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiView protein in SMART
SM00846. Gp_dh_N. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiView protein in PROSITE
PS00071. GAPDH. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiG3PB_ARATH
AccessioniPrimary (citable) accession number: P25857
Secondary accession number(s): Q0WL92, Q9C7S2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 2, 2002
Last modified: May 10, 2017
This is version 163 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.