Reviewed, UniProtKB/Swiss-Prot P25857 (G3PB_ARATH)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic
    EC=1.2.1.13
Alternative name(s):
    NADP-dependent glyceraldehydephosphate dehydrogenase subunit B

Gene names

Name:	GAPB
Ordered Locus Names:	At1g42970
ORF Names:	F13A11.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NADP+ = 3-phospho-D-glyceroyl phosphate + NADPH.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subunit structure	Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1) By similarity.
Subcellular location	Plastid › chloroplast membrane; Peripheral membrane protein. Plastid › chloroplast stroma. Ref.5
Miscellaneous	Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Calvin cycle
Cellular component	Chloroplast Membrane Plastid
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW reductive pentose-phosphate cycle Non-traceable author statement. Source: TAIR response to cadmium ion Inferred from expression pattern. Source: TAIR response to cold Inferred from expression pattern. Source: TAIR response to light stimulus Inferred from expression pattern. Source: TAIR response to sucrose stimulus Inferred from expression pattern. Source: TAIR
Cellular component	apoplast Inferred from direct assay. Source: TAIR chloroplast membrane Inferred from electronic annotation. Source: UniProtKB-SubCell chloroplast stroma Inferred from direct assay. Source: TAIR chloroplast thylakoid membrane Inferred from direct assay. Source: TAIR
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
At2g42580	Q9SIN1	1	EBI-2294858,EBI-2292882

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 80	80	Chloroplast By similarity
	Chain	81 – 447	367	Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic		PRO_0000010417
Regions
	Nucleotide binding	91 – 92	2	NADP By similarity
	Region	234 – 236	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	293 – 294	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	235	1	Nucleophile By similarity
	Binding site	115	1	NADP By similarity
	Binding site	160	1	NADP; via carbonyl oxygen By similarity
	Binding site	265	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	280	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	316	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	399	1	NADP By similarity
	Site	262	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P25857-1 [UniParc]. Last modified May 2, 2002. Version 2. Checksum: E7DBD59915560EF3	FASTA	447	47,660
	10 20 30 40 50 60 MATHAALAVS RIPVTQRLQS KSAIHSFPAQ CSSKRLEVAE FSGLRMSSIG GEASFFDAVA 70 80 90 100 110 120 AQIIPKAVTT STPVRGETVA KLKVAINGFG RIGRNFLRCW HGRKDSPLEV VVLNDSGGVK 130 140 150 160 170 180 NASHLLKYDS MLGTFKAEVK IVDNETISVD GKLIKVVSNR DPLKLPWAEL GIDIVIEGTG 190 200 210 220 230 240 VFVDGPGAGK HIQAGASKVI ITAPAKGADI PTYVMGVNEQ DYGHDVANII SNASCTTNCL 250 260 270 280 290 300 APFAKVLDEE FGIVKGTMTT THSYTGDQRL LDASHRDLRR ARAAALNIVP TSTGAAKAVS 310 320 330 340 350 360 LVLPQLKGKL NGIALRVPTP NVSVVDLVIN VEKKGLTAED VNEAFRKAAN GPMKGILDVC 370 380 390 400 410 420 DAPLVSVDFR CSDVSTTIDS SLTMVMGDDM VKVVAWYDNE WGYSQRVVDL AHLVASKWPG 430 440 AEAVGSGDPL EDFCKTNPAD EECKVYD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and chromosomal mapping of nuclear genes encoding chloroplast and cytosolic glyceraldehyde-3-phosphate-dehydrogenase from Arabidopsis thaliana." Shih M.-C., Heinrich P., Goodman H.M. Gene 104:133-138(1991) [PubMed: 1916285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Tissue: Leaf.
[2]	Erratum Shih M.-C., Heinrich P., Goodman H.M. Gene 119:317-319(1992) [PubMed: 1398114] [Abstract]
[3]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana." Ferro M., Salvi D., Brugiere S., Miras S., Kowalski S., Louwagie M., Garin J., Joyard J., Rolland N. Mol. Cell. Proteomics 2:325-345(2003) [PubMed: 12766230] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M64115 mRNA. Translation: AAD10210.1. M64118 Genomic DNA. Translation: AAA32795.1. AC068324 Genomic DNA. Translation: AAG51517.1. AY039961 mRNA. Translation: AAK64065.1. AY039539 mRNA. Translation: AAK62594.1. AY079402 mRNA. Translation: AAL85133.1. AY095991 mRNA. Translation: AAM19948.1. AY140091 mRNA. Translation: AAM98232.1. BT002267 mRNA. Translation: AAN72278.1.
IPI	IPI00541680.
PIR	C96497. JQ1286.
RefSeq	NP_174996.1.
UniGene	At.21749 At.67261
3D structure databases
HSSP	HSSP built from PDB template 1NBO based on UniProtKB P19866.
SMR	P25857. Positions 81-418.
ModBase	Search...
2-D gel databases
SWISS-2DPAGE	P25857.
Proteomic databases
PRIDE	P25857.
ProMEX	P25857.
Genome annotation databases
GeneID	840895.
GenomeReviews	Gene locus AT1G42970 in contig CT485782_GR.
KEGG	ath:AT1G42970.
Organism-specific databases
TAIR	At1g42970.
Phylogenomic databases
OMA	P25857. YDSTFGV.
Enzyme and pathway databases
BRENDA	1.2.1.13. 302.
Gene expression databases
ArrayExpress	P25857.
GermOnline	AT1G42970. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DH. IPR006424. Glyceraldehyde-3-P_DH_1. IPR003823. Unk_CP12. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02672. CP12. 1 hit. PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3PB_ARATH

Accession

Primary (citable) accession number: P25857
Secondary accession number(s): Q9C7S2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 2, 2002
Last modified:	June 16, 2009
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)