P25857 (G3PB_ARATH) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 119.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic EC=1.2.1.13 Alternative name(s): NADP-dependent glyceraldehydephosphate dehydrogenase subunit B | ||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Protein attributes
| Sequence length | 447 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Catalytic activity | D-glyceraldehyde 3-phosphate + phosphate + NADP+ = 3-phospho-D-glyceroyl phosphate + NADPH. |
| Pathway | |
| Subunit structure | Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1) By similarity. |
| Subcellular location | Plastid › chloroplast membrane; Peripheral membrane protein. Plastid › chloroplast stroma Ref.6. |
| Miscellaneous | Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes. |
| Sequence similarities | Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 80 | 80 | Chloroplast By similarity | ||||||
| Chain | 81 – 447 | 367 | Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic | PRO_0000010417 | |||||
Regions | |||||||||
| Nucleotide binding | 91 – 92 | 2 | NADP By similarity | ||||||
| Region | 234 – 236 | 3 | Glyceraldehyde 3-phosphate binding By similarity | ||||||
| Region | 293 – 294 | 2 | Glyceraldehyde 3-phosphate binding By similarity | ||||||
Sites | |||||||||
| Active site | 235 | 1 | Nucleophile By similarity | ||||||
| Binding site | 115 | 1 | NADP By similarity | ||||||
| Binding site | 160 | 1 | NADP; via carbonyl oxygen By similarity | ||||||
| Binding site | 265 | 1 | Glyceraldehyde 3-phosphate By similarity | ||||||
| Binding site | 280 | 1 | Glyceraldehyde 3-phosphate By similarity | ||||||
| Binding site | 316 | 1 | Glyceraldehyde 3-phosphate By similarity | ||||||
| Binding site | 399 | 1 | NADP By similarity | ||||||
| Site | 262 | 1 | Activates thiol group during catalysis By similarity | ||||||
Sequences
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References
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | M64115 mRNA. Translation: AAD10210.1. M64118 Genomic DNA. Translation: AAA32795.1. AC068324 Genomic DNA. Translation: AAG51517.1. CP002684 Genomic DNA. Translation: AEE31933.1. AY039961 mRNA. Translation: AAK64065.1. AY039539 mRNA. Translation: AAK62594.1. AY079402 mRNA. Translation: AAL85133.1. AY095991 mRNA. Translation: AAM19948.1. AY140091 mRNA. Translation: AAM98232.1. BT002267 mRNA. Translation: AAN72278.1. |
| IPI | IPI00541680. |
| PIR | C96497. JQ1286. |
| RefSeq | NP_174996.1. NM_103456.3. |
| UniGene | At.21749. At.67261. |
3D structure databases | |
| ProteinModelPortal | P25857. |
| SMR | P25857. Positions 81-422. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P25857. 2 interactions. |
| STRING | P25857. |
2D gel databases | |
| SWISS-2DPAGE | P25857. |
Proteomic databases | |
| PRIDE | P25857. |
| ProMEX | P25857. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblPlants | AT1G42970.1; AT1G42970.1; AT1G42970. |
| GeneID | 840895. |
| GenomeReviews | Gene locus AT1G42970 in contig CT485782_GR. |
| KEGG | ath:AT1G42970. |
Organism-specific databases | |
| TAIR | At1g42970. |
Phylogenomic databases | |
| eggNOG | KOG0657. |
| HOGENOM | HBG571736. |
| InParanoid | P25857. |
| OMA | IGRNAFK. |
| PhylomeDB | P25857. |
| ProtClustDB | PLN02237. |
Enzyme and pathway databases | |
| BRENDA | 1.2.1.13. 399. |
Gene expression databases | |
| ArrayExpress | P25857. |
| Genevestigator | P25857. |
| GermOnline | AT1G42970. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR003823. DUF_CP12. IPR020831. GlycerAld/Erythrose_P_DH. IPR020830. GlycerAld_3-P_DH_AS. IPR020829. GlycerAld_3-P_DH_cat. IPR020828. GlycerAld_3-P_DH_NAD(P)-bd. IPR006424. Glyceraldehyde-3-P_DH_1. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| KO | K05298. |
| PANTHER | PTHR10836. GAP_DH. 1 hit. |
| Pfam | PF02672. CP12. 1 hit. PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view] |
| PRINTS | PR00078. G3PDHDRGNASE. |
| SMART | SM00846. Gp_dh_N. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01534. GAPDH-I. 1 hit. |
| PROSITE | PS00071. GAPDH. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | G3PB_ARATH | ||||||||
| Accession | Primary (citable) accession number: P25857 Secondary accession number(s): Q9C7S2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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