Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic precursor

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P25856 (G3PA_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 135. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic
EC=1.2.1.13

Alternative name(s):
NADP-dependent glyceraldehydephosphate dehydrogenase subunit A

Gene names

Name:	GAPA
Ordered Locus Names:	At3g26650
ORF Names:	MLJ15.4, MLJ15_5

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NADP⁺ = 3-phospho-D-glyceroyl phosphate + NADPH.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subunit structure	Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1). Ref.8 Ref.9
Subcellular location	Plastid › chloroplast membrane; Peripheral membrane protein. Plastid › chloroplast stroma Ref.7.
Miscellaneous	Plants contain two types of GAPDH: cytosolic forms which participate in glycolysis and chloroplast forms which participate in photosynthesis. All the forms are encoded by distinct genes.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
Sequence caution	The sequence AAD10209.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Calvin cycle
Cellular component	Chloroplast Membrane Plastid
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glucose metabolic process Inferred from electronic annotation. Source: InterPro reductive pentose-phosphate cycle Non-traceable author statement PubMed 15533878. Source: TAIR response to cold Inferred from expression pattern PubMed 16923014. Source: TAIR response to light stimulus Inferred from expression pattern PubMed 15533878. Source: TAIR response to sucrose stimulus Inferred from expression pattern PubMed 15533878. Source: TAIR
Cellular_component	apoplast Inferred from direct assay PubMed 18538804. Source: TAIR chloroplast envelope Inferred from direct assay PubMed 20061580. Source: TAIR chloroplast membrane Inferred from electronic annotation. Source: UniProtKB-SubCell chloroplast stroma Inferred from direct assay PubMed 16207701 PubMed 18633119 PubMed 20061580. Source: TAIR chloroplast thylakoid membrane Inferred from direct assay PubMed 15322131. Source: TAIR stromule Inferred from direct assay PubMed 16923014. Source: TAIR
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CP12-2	Q9LZP9	4	EBI-1554434,EBI-449218

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 60

Chloroplast By similarity

Chain

61 – 396

336

Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic

PRO_0000010416

Regions

Nucleotide binding

71 – 72

NADP

Region

212 – 214

Glyceraldehyde 3-phosphate binding By similarity

Region

271 – 272

Glyceraldehyde 3-phosphate binding By similarity

Sites

Active site

213

Nucleophile By similarity

Binding site

NADP

Binding site

140

NADP; via carbonyl oxygen

Binding site

243

Glyceraldehyde 3-phosphate By similarity

Binding site

258

Glyceraldehyde 3-phosphate By similarity

Binding site

294

Glyceraldehyde 3-phosphate By similarity

Binding site

376

NADP

Site

240

Activates thiol group during catalysis By similarity

Experimental info

Sequence conflict

172

I → M in AAA32793. Ref.1

Sequence conflict

172

I → M in AAD10209. Ref.1

Sequence conflict

172

I → M in CAA66816. Ref.3

Secondary structure

396

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P25856 [UniParc].

Last modified May 2, 2002. Version 3.
Checksum: FFD7FD662FB222FC
Show »

FASTA

396

42,490

        10         20         30         40         50         60 
MASVTFSVPK GFTEFSGLRS SSASLPFGKK LSSDEFVSIV SFQTSAMGSS GGYRKGVTEA 

        70         80         90        100        110        120 
KLKVAINGFG RIGRNFLRCW HGRKDSPLDI IAINDTGGVK QASHLLKYDS TLGIFDADVK 

       130        140        150        160        170        180 
PSGETAISVD GKIIQVVSNR NPSLLPWKEL GIDIVIEGTG VFVDREGAGK HIEAGAKKVI 

       190        200        210        220        230        240 
ITAPGKGDIP TYVVGVNADA YSHDEPIISN ASCTTNCLAP FVKVLDQKFG IIKGTMTTTH 

       250        260        270        280        290        300 
SYTGDQRLLD ASHRDLRRAR AAALNIVPTS TGAAKAVALV LPNLKGKLNG IALRVPTPNV 

       310        320        330        340        350        360 
SVVDLVVQVS KKTFAEEVNA AFRDSAEKEL KGILDVCDEP LVSVDFRCSD FSTTIDSSLT 

       370        380        390 
MVMGDDMVKV IAWYDNEWGY SQRVVDLADI VANNWK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and chromosomal mapping of nuclear genes encoding chloroplast and cytosolic glyceraldehyde-3-phosphate-dehydrogenase from Arabidopsis thaliana." Shih M.-C., Heinrich P., Goodman H.M. Gene 104:133-138(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	Erratum Shih M.-C., Heinrich P., Goodman H.M. Gene 119:317-319(1992) [PubMed] [Europe PMC] [Abstract]
[3]	"Sequence analysis of an 81 kb contig from Arabidopsis thaliana chromosome III." Quigley F., Dao P., Cottet A., Mache R. Nucleic Acids Res. 24:4313-4318(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. Columbia. Tissue: Shoot.
[4]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[6]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[7]	"Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana." Ferro M., Salvi D., Brugiere S., Miras S., Kowalski S., Louwagie M., Garin J., Joyard J., Rolland N. Mol. Cell. Proteomics 2:325-345(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: cv. Wassilewskija.
[8]	"Structure of photosynthetic glyceraldehyde-3-phosphate dehydrogenase (isoform A4) from Arabidopsis thaliana in complex with NAD." Fermani S., Sparla F., Marri L., Thumiger A., Pupillo P., Falini G., Trost P. Acta Crystallogr. F 66:621-626(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) OF 60-396 IN COMPLEX WITH NAD, SUBUNIT.
[9]	"Conformational selection and folding-upon-binding of intrinsically disordered protein CP12 regulate photosynthetic enzymes assembly." Fermani S., Trivelli X., Sparla F., Thumiger A., Calvaresi M., Marri L., Falini G., Zerbetto F., Trost P. J. Biol. Chem. 287:21372-21383(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 60-396 IN COMPLEX WITH NAD, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M64114 mRNA. Translation: AAD10209.1. Different initiation.
M64117 Genomic DNA. Translation: AAA32793.1.
S45910 Genomic DNA. Translation: AAB23532.1.
X98130 Genomic DNA. Translation: CAA66816.1.
AB026648 Genomic DNA. Translation: BAB01730.1.
CP002686 Genomic DNA. Translation: AEE77191.1.
AY058140 mRNA. Translation: AAL25556.1.
AY058107 mRNA. Translation: AAL24215.1.
AF428431 mRNA. Translation: AAL16200.1.
AY075637 mRNA. Translation: AAL91645.1.
AY142053 mRNA. Translation: AAM98317.1.

IPI

IPI00537303.

PIR

JQ1285.

RefSeq

NP_566796.2. NM_113576.3.

UniGene

At.20459.
At.23357.
At.3405.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3K2B	X-ray	2.60	A/B/C/D/E/F/G/H/O/Q	60-396	[»]
3QV1	X-ray	2.00	A/B/C/D/E/F	60-396	[»]
3RVD	X-ray	2.70	A/B/C/D/E/F/G/H/O/Q	60-396	[»]

ProteinModelPortal

P25856.

SMR

P25856. Positions 60-396.

ModBase

Search...

Protein-protein interaction databases

IntAct

P25856. 1 interaction.

2D gel databases

SWISS-2DPAGE

P25856.

World-2DPAGE

0003:P25856.

Proteomic databases

PaxDb

P25856.

PRIDE

P25856.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

AT3G26650.1; AT3G26650.1; AT3G26650.

GeneID

822277.

KEGG

ath:AT3G26650.

Organism-specific databases

TAIR

At3g26650.

Phylogenomic databases

eggNOG

COG0057.

HOGENOM

HOG000071679.

InParanoid

P25856.

K05298.

OMA

TDEKASM.

PhylomeDB

P25856.

ProtClustDB

PLN03096.

Enzyme and pathway databases

BioCyc

ARA:AT3G26650-MONOMER.
MetaCyc:AT3G26650-MONOMER.

BRENDA

1.2.1.13. 399.

UniPathway

UPA00116.

Gene expression databases

ArrayExpress

P25856.

Genevestigator

P25856.

GermOnline

AT3G26650. Arabidopsis thaliana.

Family and domain databases

Gene3D

3.40.50.720. 1 hit.

InterPro

IPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]

PANTHER

PTHR10836. PTHR10836. 1 hit.

Pfam

PF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]

PIRSF

PIRSF000149. GAP_DH. 1 hit.

PRINTS

PR00078. G3PDHDRGNASE.

SMART

SM00846. Gp_dh_N. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01534. GAPDH-I. 1 hit.

PROSITE

PS00071. GAPDH. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P25856.

Entry information

Entry name

G3PA_ARATH

Accession

Primary (citable) accession number: P25856
Secondary accession number(s): Q41184, Q9LSE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 2, 2002
Last modified:	May 1, 2013
This is version 135 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents