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Protein

Glyceraldehyde-3-phosphate dehydrogenase GAPA1, chloroplastic

Gene

GAPA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the photosynthetic reductive pentose phosphate pathway (Calvin-Benson cycle). Catalyzes the reduction of 1,3-diphosphoglycerate by NADPH (By similarity).By similarity

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NADP+ = 3-phospho-D-glyceroyl phosphate + NADPH.

Pathwayi: Calvin cycle

This protein is involved in the pathway Calvin cycle, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway Calvin cycle and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei95NADP2 Publications1
Binding sitei140NADP; via carbonyl oxygen2 Publications1
Active sitei213NucleophilePROSITE-ProRule annotation1
Sitei240Activates thiol group during catalysisBy similarity1
Binding sitei243Glyceraldehyde 3-phosphateBy similarity1
Binding sitei258Glyceraldehyde 3-phosphateBy similarity1
Binding sitei294Glyceraldehyde 3-phosphateBy similarity1
Binding sitei376NADP2 Publications1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi71 – 72NADP2 Publications2

GO - Molecular functioni

GO - Biological processi

  • glucose metabolic process Source: InterPro
  • reductive pentose-phosphate cycle Source: TAIR
  • response to cold Source: TAIR
  • response to light stimulus Source: TAIR
  • response to sucrose Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Calvin cycle

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciARA:AT3G26650-MONOMER.
MetaCyc:AT3G26650-MONOMER.
BRENDAi1.2.1.13. 399.
UniPathwayiUPA00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase GAPA1, chloroplastic (EC:1.2.1.13)
Alternative name(s):
NADP-dependent glyceraldehydephosphate dehydrogenase A subunit 1
Gene namesi
Name:GAPA1
Synonyms:GAPA
Ordered Locus Names:At3g26650
ORF Names:MLJ15.4, MLJ15_5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G26650.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast membrane Source: UniProtKB-SubCell
  • chloroplast stroma Source: TAIR
  • chloroplast thylakoid membrane Source: TAIR
  • membrane Source: TAIR
  • stromule Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 60ChloroplastBy similarityAdd BLAST60
ChainiPRO_000001041661 – 396Glyceraldehyde-3-phosphate dehydrogenase GAPA1, chloroplasticAdd BLAST336

Proteomic databases

PaxDbiP25856.
PRIDEiP25856.

2D gel databases

SWISS-2DPAGEP25856.
World-2DPAGE0003:P25856.

PTM databases

iPTMnetiP25856.

Expressioni

Tissue specificityi

Expressed in leaves and stems.1 Publication

Inductioni

Repressed by darkness and sucrose.1 Publication

Gene expression databases

GenevisibleiP25856. AT.

Interactioni

Subunit structurei

Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CP12-2Q9LZP94EBI-1554434,EBI-449218

Protein-protein interaction databases

BioGridi7607. 7 interactors.
IntActiP25856. 2 interactors.
STRINGi3702.AT3G26650.1.

Structurei

Secondary structure

1396
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi62 – 68Combined sources7
Helixi71 – 81Combined sources11
Beta strandi87 – 94Combined sources8
Helixi99 – 107Combined sources9
Turni110 – 112Combined sources3
Beta strandi119 – 129Combined sources11
Beta strandi132 – 137Combined sources6
Helixi142 – 144Combined sources3
Helixi147 – 150Combined sources4
Beta strandi154 – 157Combined sources4
Beta strandi159 – 161Combined sources3
Helixi165 – 173Combined sources9
Beta strandi177 – 183Combined sources7
Beta strandi186 – 188Combined sources3
Turni194 – 196Combined sources3
Helixi198 – 200Combined sources3
Beta strandi206 – 209Combined sources4
Helixi213 – 229Combined sources17
Beta strandi231 – 241Combined sources11
Beta strandi248 – 250Combined sources3
Turni256 – 259Combined sources4
Helixi262 – 264Combined sources3
Beta strandi267 – 269Combined sources3
Helixi273 – 280Combined sources8
Helixi282 – 284Combined sources3
Turni285 – 287Combined sources3
Beta strandi288 – 296Combined sources9
Beta strandi301 – 311Combined sources11
Helixi315 – 327Combined sources13
Turni328 – 333Combined sources6
Beta strandi334 – 337Combined sources4
Helixi343 – 346Combined sources4
Beta strandi352 – 356Combined sources5
Helixi357 – 359Combined sources3
Beta strandi361 – 363Combined sources3
Turni364 – 366Combined sources3
Beta strandi367 – 374Combined sources8
Helixi378 – 393Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3K2BX-ray2.60A/B/C/D/E/F/G/H/O/Q60-396[»]
3QV1X-ray2.00A/B/C/D/E/F60-396[»]
3RVDX-ray2.70A/B/C/D/E/F/G/H/O/Q61-396[»]
ProteinModelPortaliP25856.
SMRiP25856.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25856.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni212 – 214Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni271 – 272Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
HOGENOMiHOG000071679.
InParanoidiP25856.
KOiK05298.
OMAiDFNHTEH.
OrthoDBiEOG09360CQC.
PhylomeDBiP25856.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25856-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVTFSVPK GFTEFSGLRS SSASLPFGKK LSSDEFVSIV SFQTSAMGSS
60 70 80 90 100
GGYRKGVTEA KLKVAINGFG RIGRNFLRCW HGRKDSPLDI IAINDTGGVK
110 120 130 140 150
QASHLLKYDS TLGIFDADVK PSGETAISVD GKIIQVVSNR NPSLLPWKEL
160 170 180 190 200
GIDIVIEGTG VFVDREGAGK HIEAGAKKVI ITAPGKGDIP TYVVGVNADA
210 220 230 240 250
YSHDEPIISN ASCTTNCLAP FVKVLDQKFG IIKGTMTTTH SYTGDQRLLD
260 270 280 290 300
ASHRDLRRAR AAALNIVPTS TGAAKAVALV LPNLKGKLNG IALRVPTPNV
310 320 330 340 350
SVVDLVVQVS KKTFAEEVNA AFRDSAEKEL KGILDVCDEP LVSVDFRCSD
360 370 380 390
FSTTIDSSLT MVMGDDMVKV IAWYDNEWGY SQRVVDLADI VANNWK
Length:396
Mass (Da):42,490
Last modified:May 2, 2002 - v3
Checksum:iFFD7FD662FB222FC
GO

Sequence cautioni

The sequence AAD10209 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti172I → M in AAA32793 (PubMed:1916285).Curated1
Sequence conflicti172I → M in AAD10209 (PubMed:1916285).Curated1
Sequence conflicti172I → M in CAA66816 (PubMed:8932388).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64114 mRNA. Translation: AAD10209.1. Different initiation.
M64117 Genomic DNA. Translation: AAA32793.1.
S45910 Genomic DNA. Translation: AAB23532.1.
X98130 Genomic DNA. Translation: CAA66816.1.
AB026648 Genomic DNA. Translation: BAB01730.1.
CP002686 Genomic DNA. Translation: AEE77191.1.
AY058140 mRNA. Translation: AAL25556.1.
AY058107 mRNA. Translation: AAL24215.1.
AF428431 mRNA. Translation: AAL16200.1.
AY075637 mRNA. Translation: AAL91645.1.
AY142053 mRNA. Translation: AAM98317.1.
PIRiJQ1285.
RefSeqiNP_566796.2. NM_113576.4.
UniGeneiAt.20459.
At.23357.
At.3405.

Genome annotation databases

EnsemblPlantsiAT3G26650.1; AT3G26650.1; AT3G26650.
GeneIDi822277.
GrameneiAT3G26650.1; AT3G26650.1; AT3G26650.
KEGGiath:AT3G26650.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64114 mRNA. Translation: AAD10209.1. Different initiation.
M64117 Genomic DNA. Translation: AAA32793.1.
S45910 Genomic DNA. Translation: AAB23532.1.
X98130 Genomic DNA. Translation: CAA66816.1.
AB026648 Genomic DNA. Translation: BAB01730.1.
CP002686 Genomic DNA. Translation: AEE77191.1.
AY058140 mRNA. Translation: AAL25556.1.
AY058107 mRNA. Translation: AAL24215.1.
AF428431 mRNA. Translation: AAL16200.1.
AY075637 mRNA. Translation: AAL91645.1.
AY142053 mRNA. Translation: AAM98317.1.
PIRiJQ1285.
RefSeqiNP_566796.2. NM_113576.4.
UniGeneiAt.20459.
At.23357.
At.3405.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3K2BX-ray2.60A/B/C/D/E/F/G/H/O/Q60-396[»]
3QV1X-ray2.00A/B/C/D/E/F60-396[»]
3RVDX-ray2.70A/B/C/D/E/F/G/H/O/Q61-396[»]
ProteinModelPortaliP25856.
SMRiP25856.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7607. 7 interactors.
IntActiP25856. 2 interactors.
STRINGi3702.AT3G26650.1.

PTM databases

iPTMnetiP25856.

2D gel databases

SWISS-2DPAGEP25856.
World-2DPAGE0003:P25856.

Proteomic databases

PaxDbiP25856.
PRIDEiP25856.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G26650.1; AT3G26650.1; AT3G26650.
GeneIDi822277.
GrameneiAT3G26650.1; AT3G26650.1; AT3G26650.
KEGGiath:AT3G26650.

Organism-specific databases

TAIRiAT3G26650.

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
HOGENOMiHOG000071679.
InParanoidiP25856.
KOiK05298.
OMAiDFNHTEH.
OrthoDBiEOG09360CQC.
PhylomeDBiP25856.

Enzyme and pathway databases

UniPathwayiUPA00116.
BioCyciARA:AT3G26650-MONOMER.
MetaCyc:AT3G26650-MONOMER.
BRENDAi1.2.1.13. 399.

Miscellaneous databases

EvolutionaryTraceiP25856.
PROiP25856.

Gene expression databases

GenevisibleiP25856. AT.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3PA1_ARATH
AccessioniPrimary (citable) accession number: P25856
Secondary accession number(s): Q41184, Q9LSE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 2, 2002
Last modified: November 30, 2016
This is version 164 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants contain three types of GAPDH: NAD-dependent cytosolic forms which participate in glycolysis, NAD-dependent chloroplastic forms which participate in plastidic glycolysis and NADP-dependent chloroplastic forms which participate in the photosynthetic reductive pentose phosphate pathway (Calvin-Benson cycle). All the forms are encoded by distinct genes.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.