Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic precursor

Contribute

Send feedback

Read comments (1) or add your own

Reviewed, UniProtKB/Swiss-Prot P25856 (G3PA_ARATH)

Last modified June 16, 2009. Version 94. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic
    EC=1.2.1.13
Alternative name(s):
    NADP-dependent glyceraldehydephosphate dehydrogenase subunit A

Gene names

Name:	GAPA
Ordered Locus Names:	At3g26650
ORF Names:	MLJ15.4, MLJ15_5

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Hide | Top

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

Hide | Top

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NADP⁺ = 3-phospho-D-glyceroyl phosphate + NADPH.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subunit structure	Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1) By similarity.
Subcellular location	Plastid › chloroplast membrane; Peripheral membrane protein. Plastid › chloroplast stroma. Ref.6
Miscellaneous	Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Hide | Top

Ontologies

Keywords
Biological process	Calvin cycle
Cellular component	Chloroplast Membrane Plastid
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW reductive pentose-phosphate cycle Non-traceable author statement. Source: TAIR response to cold Inferred from expression pattern. Source: TAIR response to light stimulus Inferred from expression pattern. Source: TAIR response to sucrose stimulus Inferred from expression pattern. Source: TAIR
Cellular component	apoplast Inferred from direct assay. Source: TAIR chloroplast membrane Inferred from electronic annotation. Source: UniProtKB-SubCell chloroplast stroma Inferred from direct assay. Source: TAIR chloroplast thylakoid membrane Inferred from direct assay. Source: TAIR
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: TAIR
Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 60

Chloroplast By similarity

Chain

61 – 396

336

Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic

PRO_0000010416

Regions

Nucleotide binding

71 – 72

NADP By similarity

Region

212 – 214

Glyceraldehyde 3-phosphate binding By similarity

Region

271 – 272

Glyceraldehyde 3-phosphate binding By similarity

Sites

Active site

213

Nucleophile By similarity

Binding site

NADP By similarity

Binding site

140

NADP; via carbonyl oxygen By similarity

Binding site

243

Glyceraldehyde 3-phosphate By similarity

Binding site

258

Glyceraldehyde 3-phosphate By similarity

Binding site

294

Glyceraldehyde 3-phosphate By similarity

Binding site

376

NADP By similarity

Site

240

Activates thiol group during catalysis By similarity

Experimental info

Sequence conflict

172

I → M Ref.1

Sequence conflict

172

I → M Ref.3

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

P25856-1 [UniParc].

Last modified May 2, 2002. Version 3.
Checksum: FFD7FD662FB222FC
Show »

FASTA

396

42,490

        10         20         30         40         50         60 
MASVTFSVPK GFTEFSGLRS SSASLPFGKK LSSDEFVSIV SFQTSAMGSS GGYRKGVTEA 

        70         80         90        100        110        120 
KLKVAINGFG RIGRNFLRCW HGRKDSPLDI IAINDTGGVK QASHLLKYDS TLGIFDADVK 

       130        140        150        160        170        180 
PSGETAISVD GKIIQVVSNR NPSLLPWKEL GIDIVIEGTG VFVDREGAGK HIEAGAKKVI 

       190        200        210        220        230        240 
ITAPGKGDIP TYVVGVNADA YSHDEPIISN ASCTTNCLAP FVKVLDQKFG IIKGTMTTTH 

       250        260        270        280        290        300 
SYTGDQRLLD ASHRDLRRAR AAALNIVPTS TGAAKAVALV LPNLKGKLNG IALRVPTPNV 

       310        320        330        340        350        360 
SVVDLVVQVS KKTFAEEVNA AFRDSAEKEL KGILDVCDEP LVSVDFRCSD FSTTIDSSLT 

       370        380        390 
MVMGDDMVKV IAWYDNEWGY SQRVVDLADI VANNWK

« Hide

Hide | Top

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and chromosomal mapping of nuclear genes encoding chloroplast and cytosolic glyceraldehyde-3-phosphate-dehydrogenase from Arabidopsis thaliana." Shih M.-C., Heinrich P., Goodman H.M. Gene 104:133-138(1991) [PubMed: 1916285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	Erratum Shih M.-C., Heinrich P., Goodman H.M. Gene 119:317-319(1992) [PubMed: 1398114] [Abstract]
[3]	"Sequence analysis of an 81 kb contig from Arabidopsis thaliana chromosome III." Quigley F., Dao P., Cottet A., Mache R. Nucleic Acids Res. 24:4313-4318(1996) [PubMed: 8932388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. Columbia. Tissue: Shoot.
[4]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana." Ferro M., Salvi D., Brugiere S., Miras S., Kowalski S., Louwagie M., Garin J., Joyard J., Rolland N. Mol. Cell. Proteomics 2:325-345(2003) [PubMed: 12766230] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Hide | Top

Cross-references

Sequence databases
	M64114 mRNA. Translation: AAD10209.1. Different initiation. M64117 Genomic DNA. Translation: AAA32793.1. S45910 Genomic DNA. Translation: AAB23532.1. X98130 Genomic DNA. Translation: CAA66816.1. AB026648 Genomic DNA. Translation: BAB01730.1. AY058140 mRNA. Translation: AAL25556.1. AY058107 mRNA. Translation: AAL24215.1. AF428431 mRNA. Translation: AAL16200.1. AY075637 mRNA. Translation: AAL91645.1. AY142053 mRNA. Translation: AAM98317.1.
IPI	IPI00537303.
PIR	JQ1285.
RefSeq	NP_566796.2.
UniGene	At.20459 At.23357 At.71545 At.71588
3D structure databases
HSSP	HSSP built from PDB template 1NBO based on UniProtKB P19866.
SMR	P25856. Positions 61-396.
ModBase	Search...
2-D gel databases
SWISS-2DPAGE	P25856.
Proteomic databases
PRIDE	P25856.
ProMEX	P25856.
Genome annotation databases
GeneID	822277.
GenomeReviews	Gene locus AT3G26650 in contig BA000014_GR.
KEGG	ath:AT3G26650.
NMPDR	fig\|3702.1.peg.14942.
Organism-specific databases
TAIR	At3g26650.
Phylogenomic databases
OMA	P25856. EAFKVEN.
Enzyme and pathway databases
BioCyc	MetaCyc:AT3G26650-MON.
BRENDA	1.2.1.13. 302.
Gene expression databases
ArrayExpress	P25856.
GermOnline	AT3G26650. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DH. IPR006424. Glyceraldehyde-3-P_DH_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Hide | Top

Entry information

Entry name

G3PA_ARATH

Accession

Primary (citable) accession number: P25856
Secondary accession number(s): Q41184, Q9LSE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 2, 2002
Last modified:	June 16, 2009
This is version 94 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

Hide | Top

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

2-D gel databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents