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Reviewed, UniProtKB/Swiss-Prot P25856 (G3PA_ARATH)

Last modified June 16, 2009. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic
    EC=1.2.1.13
Alternative name(s):
    NADP-dependent glyceraldehydephosphate dehydrogenase subunit A
Gene names
Name: GAPA
Ordered Locus Names: At3g26650
ORF Names: MLJ15.4, MLJ15_5
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidseurosids IIBrassicalesBrassicaceaeArabidopsis

Protein attributes

Sequence length396 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

D-glyceraldehyde 3-phosphate + phosphate + NADP+ = 3-phospho-D-glyceroyl phosphate + NADPH.

Pathway

Carbohydrate biosynthesis; Calvin cycle.

Subunit structure

Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1) By similarity.

Subcellular location

Plastidchloroplast membrane; Peripheral membrane protein. Plastidchloroplast stroma. Ref.6

Miscellaneous

Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes.

Sequence similarities

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 6060Chloroplast By similarity
Chain61 – 396336Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic
PRO_0000010416

Regions

Nucleotide binding71 – 722NADP By similarity
Region212 – 2143Glyceraldehyde 3-phosphate binding By similarity
Region271 – 2722Glyceraldehyde 3-phosphate binding By similarity

Sites

Active site2131Nucleophile By similarity
Binding site951NADP By similarity
Binding site1401NADP; via carbonyl oxygen By similarity
Binding site2431Glyceraldehyde 3-phosphate By similarity
Binding site2581Glyceraldehyde 3-phosphate By similarity
Binding site2941Glyceraldehyde 3-phosphate By similarity
Binding site3761NADP By similarity
Site2401Activates thiol group during catalysis By similarity

Experimental info

Sequence conflict1721I → M Ref.1
Sequence conflict1721I → M Ref.3

Sequences

Sequence LengthMass (Da)Tools
P25856-1 [UniParc].

Last modified May 2, 2002. Version 3.
Checksum: FFD7FD662FB222FC

FASTA39642,490
        10         20         30         40         50         60 
MASVTFSVPK GFTEFSGLRS SSASLPFGKK LSSDEFVSIV SFQTSAMGSS GGYRKGVTEA 

        70         80         90        100        110        120 
KLKVAINGFG RIGRNFLRCW HGRKDSPLDI IAINDTGGVK QASHLLKYDS TLGIFDADVK 

       130        140        150        160        170        180 
PSGETAISVD GKIIQVVSNR NPSLLPWKEL GIDIVIEGTG VFVDREGAGK HIEAGAKKVI 

       190        200        210        220        230        240 
ITAPGKGDIP TYVVGVNADA YSHDEPIISN ASCTTNCLAP FVKVLDQKFG IIKGTMTTTH 

       250        260        270        280        290        300 
SYTGDQRLLD ASHRDLRRAR AAALNIVPTS TGAAKAVALV LPNLKGKLNG IALRVPTPNV 

       310        320        330        340        350        360 
SVVDLVVQVS KKTFAEEVNA AFRDSAEKEL KGILDVCDEP LVSVDFRCSD FSTTIDSSLT 

       370        380        390 
MVMGDDMVKV IAWYDNEWGY SQRVVDLADI VANNWK 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and chromosomal mapping of nuclear genes encoding chloroplast and cytosolic glyceraldehyde-3-phosphate-dehydrogenase from Arabidopsis thaliana."
Shih M.-C., Heinrich P., Goodman H.M.
Gene 104:133-138(1991) [PubMed: 1916285] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]Erratum
Shih M.-C., Heinrich P., Goodman H.M.
Gene 119:317-319(1992) [PubMed: 1398114] [Abstract]
[3]"Sequence analysis of an 81 kb contig from Arabidopsis thaliana chromosome III."
Quigley F., Dao P., Cottet A., Mache R.
Nucleic Acids Res. 24:4313-4318(1996) [PubMed: 8932388] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: cv. Columbia.
Tissue: Shoot.
[4]"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana."
Ferro M., Salvi D., Brugiere S., Miras S., Kowalski S., Louwagie M., Garin J., Joyard J., Rolland N.
Mol. Cell. Proteomics 2:325-345(2003) [PubMed: 12766230] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases

M64114 mRNA. Translation: AAD10209.1. Different initiation.
M64117 Genomic DNA. Translation: AAA32793.1.
S45910 Genomic DNA. Translation: AAB23532.1.
X98130 Genomic DNA. Translation: CAA66816.1.
AB026648 Genomic DNA. Translation: BAB01730.1.
AY058140 mRNA. Translation: AAL25556.1.
AY058107 mRNA. Translation: AAL24215.1.
AF428431 mRNA. Translation: AAL16200.1.
AY075637 mRNA. Translation: AAL91645.1.
AY142053 mRNA. Translation: AAM98317.1.
IPIIPI00537303.
PIRJQ1285.
RefSeqNP_566796.2.
UniGeneAt.20459
At.23357
At.71545
At.71588

3D structure databases

HSSPHSSP built from PDB template 1NBO based on UniProtKB P19866.
SMRP25856. Positions 61-396.
ModBaseSearch...

2-D gel databases

SWISS-2DPAGEP25856.

Proteomic databases

PRIDEP25856.
ProMEXP25856.

Genome annotation databases

GeneID822277.
GenomeReviewsGene locus AT3G26650 in contig BA000014_GR.
KEGGath:AT3G26650.
NMPDRfig|3702.1.peg.14942.

Organism-specific databases

TAIRAt3g26650.

Phylogenomic databases

OMAP25856. EAFKVEN.

Enzyme and pathway databases

BioCycMetaCyc:AT3G26650-MON.
BRENDA1.2.1.13. 302.

Gene expression databases

ArrayExpressP25856.
GermOnlineAT3G26650. Arabidopsis thaliana.

Family and domain databases

InterProIPR000173. GlycerAld_3-P_DH.
IPR006424. Glyceraldehyde-3-P_DH_1.
[Graphical view]
PANTHERPTHR10836. GAP_DH. 1 hit.
PfamPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000149. GAP_DH. 1 hit.
PRINTSPR00078. G3PDHDRGNASE.
TIGRFAMsTIGR01534. GAPDH-I. 1 hit.
PROSITEPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG3PA_ARATH
AccessionPrimary (citable) accession number: P25856
Secondary accession number(s): Q41184, Q9LSE6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 2, 2002
Last modified: June 16, 2009
This is version 94 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents