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Protein

Glycine cleavage system H protein 1, mitochondrial

Gene

GDH1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The glycine decarboxylase (GDC) or glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.

Cofactori

(R)-lipoateNote: Binds 1 lipoyl cofactor covalently.

Enzyme regulationi

Inhibited by harpin, S-nitrosoglutathione (GSNO), nitric oxide, N-ethylmaleimide and 5,5'-dithiobis-(2-nitrobenzoic acid).1 Publication

GO - Biological processi

Enzyme and pathway databases

BioCyciARA:AT2G35370-MONOMER.
MetaCyc:AT2G35370-MONOMER.
BRENDAi1.4.4.2. 399.
ReactomeiR-ATH-389661. Glyoxylate metabolism and glycine degradation.
R-ATH-6783984. Glycine degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine cleavage system H protein 1, mitochondrial
Gene namesi
Name:GDH1
Synonyms:GCDH, GDCH, GDCSH
Ordered Locus Names:At2g35370
ORF Names:T32F12.25
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G35370.
TAIRilocus:2062435. AT2G35370.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 34Mitochondrion1 PublicationAdd BLAST34
ChainiPRO_000001072935 – 165Glycine cleavage system H protein 1, mitochondrialAdd BLAST131

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei97N6-lipoyllysinePROSITE-ProRule annotationBy similarity1
Modified residuei140PhosphoserineCombined sources1

Post-translational modificationi

S-nitrosylated and/or glutathionylated at unknown positions in response to nitric oxide.1 Publication

Keywords - PTMi

Glutathionylation, Phosphoprotein, S-nitrosylation

Proteomic databases

PaxDbiP25855.
PRIDEiP25855.

PTM databases

iPTMnetiP25855.

Expressioni

Tissue specificityi

Highly expressed in leaves, lower expression in green veins, petioles, and stems, and detected in roots.1 Publication

Inductioni

Up-regulated by light.1 Publication

Gene expression databases

ExpressionAtlasiP25855. baseline and differential.
GenevisibleiP25855. AT.

Interactioni

Subunit structurei

The glycine cleavage system is composed of four proteins: P, T, L and H.

Protein-protein interaction databases

BioGridi3450. 1 interactor.
IntActiP25855. 1 interactor.
STRINGi3702.AT2G35370.1.

Structurei

3D structure databases

ProteinModelPortaliP25855.
SMRiP25855.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini56 – 138Lipoyl-bindingPROSITE-ProRule annotationAdd BLAST83

Sequence similaritiesi

Belongs to the GcvH family.Curated

Keywords - Domaini

Lipoyl, Transit peptide

Phylogenomic databases

eggNOGiKOG3373. Eukaryota.
COG0509. LUCA.
HOGENOMiHOG000239392.
KOiK02437.
OMAiMGAKEYT.
OrthoDBiEOG09360PSV.
PhylomeDBiP25855.

Family and domain databases

CDDicd06848. GCS_H. 1 hit.
HAMAPiMF_00272. GcvH. 1 hit.
InterProiView protein in InterPro
IPR003016. 2-oxoA_DH_lipoyl-BS.
IPR000089. Biotin_lipoyl.
IPR002930. GCV_H.
IPR033753. GCV_H/Fam206.
IPR017453. GCV_H_sub.
IPR011053. Single_hybrid_motif.
PANTHERiPTHR11715. PTHR11715. 1 hit.
PfamiView protein in Pfam
PF01597. GCV_H. 1 hit.
SUPFAMiSSF51230. SSF51230. 1 hit.
TIGRFAMsiTIGR00527. gcvH. 1 hit.
PROSITEiView protein in PROSITE
PS50968. BIOTINYL_LIPOYL. 1 hit.
PS00189. LIPOYL. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25855-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALRMWASST ANALKLSSSV SKSHLSPFSF SRCFSTVLEG LKYANSHEWV
60 70 80 90 100
KHEGSVATIG ITAHAQDHLG EVVFVELPED NTSVSKEKSF GAVESVKATS
110 120 130 140 150
EILSPISGEI IEVNKKLTES PGLINSSPYE DGWMIKVKPS SPAELESLMG
160
PKEYTKFCEE EDAAH
Length:165
Mass (Da):17,947
Last modified:May 1, 1992 - v1
Checksum:i48309650C88F9BE2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27144 Genomic DNA. Translation: AAA87942.1.
M82921 mRNA. Translation: AAA32802.1.
AC005314 Genomic DNA. Translation: AAC36184.1.
CP002685 Genomic DNA. Translation: AEC09100.1.
AY050446 mRNA. Translation: AAK91461.1.
AY058854 mRNA. Translation: AAL24242.1.
AY097349 mRNA. Translation: AAM19865.1.
AK228462 mRNA. Translation: BAF00389.1.
AY086345 mRNA. Translation: AAM64413.1.
PIRiG84767.
RefSeqiNP_181080.1. NM_129089.2.
UniGeneiAt.24936.

Genome annotation databases

EnsemblPlantsiAT2G35370.1; AT2G35370.1; AT2G35370.
GeneIDi818104.
GrameneiAT2G35370.1; AT2G35370.1; AT2G35370.
KEGGiath:AT2G35370.

Similar proteinsi

Entry informationi

Entry nameiGCSH1_ARATH
AccessioniPrimary (citable) accession number: P25855
Secondary accession number(s): Q0WR60
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: August 30, 2017
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families