Reviewed, UniProtKB/Swiss-Prot P25855 (GCSH1_ARATH)

Names and origin

Protein names

Recommended name:
    Glycine cleavage system H protein 1, mitochondrial

Gene names

Name:	GDCSH
Synonyms:	GCDH
Ordered Locus Names:	At2g35370
ORF Names:	T32F12.25

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	165 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.
Cofactor	Binds 1 lipoyl cofactor covalently.
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the gcvH family. Contains 1 lipoyl-binding domain.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Lipoyl Transit peptide
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from direct assay. Source: TAIR glycine cleavage complex Inferred from electronic annotation. Source: InterPro mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay. Source: TAIR thylakoid Inferred from direct assay. Source: TAIR
Molecular function	lipoic acid binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TRX3	Q42403	1	EBI-449563,EBI-449157

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 34	34	Mitochondrion By similarity
	Chain	35 – 165	131	Glycine cleavage system H protein 1, mitochondrial		PRO_0000010729
Amino acid modifications
	Modified residue	97	1	N6-lipoyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P25855-1 [UniParc]. Last modified May 1, 1992. Version 1. Checksum: 48309650C88F9BE2	FASTA	165	17,947
	10 20 30 40 50 60 MALRMWASST ANALKLSSSV SKSHLSPFSF SRCFSTVLEG LKYANSHEWV KHEGSVATIG 70 80 90 100 110 120 ITAHAQDHLG EVVFVELPED NTSVSKEKSF GAVESVKATS EILSPISGEI IEVNKKLTES 130 140 150 160 PGLINSSPYE DGWMIKVKPS SPAELESLMG PKEYTKFCEE EDAAH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Light-dependent and tissue-specific expression of the H-protein of the glycine decarboxylase complex." Srinivasan R., Oliver D.J. Plant Physiol. 109:161-168(1995) [PubMed: 7480320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U27144 Genomic DNA. Translation: AAA87942.1. M82921 mRNA. Translation: AAA32802.1. AC005314 Genomic DNA. Translation: AAC36184.1. AY050446 mRNA. Translation: AAK91461.1. AY058854 mRNA. Translation: AAL24242.1. AY097349 mRNA. Translation: AAM19865.1. AY086345 mRNA. Translation: AAM64413.1.
IPI	IPI00536157.
PIR	G84767.
RefSeq	NP_181080.1.
UniGene	At.24936
3D structure databases
HSSP	HSSP built from PDB template 1HTP based on UniProtKB P16048.
SMR	P25855. Positions 35-165.
ModBase	Search...
Protein-protein interaction databases
IntAct	P25855. 1 interaction.
Proteomic databases
PRIDE	P25855.
ProMEX	P25855.
Genome annotation databases
GeneID	818104.
GenomeReviews	Gene locus AT2G35370 in contig CT485783_GR.
KEGG	ath:AT2G35370.
Organism-specific databases
TAIR	At2g35370.
Phylogenomic databases
OMA	P25855. ITKYAAD.
Gene expression databases
ArrayExpress	P25855.
GermOnline	AT2G35370. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003016. 2-oxoA_DH_lipoyl-BS. IPR002930. GCV_H. IPR017453. GCV_H_sub. [Graphical view]
PANTHER	PTHR11715. GCV_H. 1 hit.
Pfam	PF01597. GCV_H. 1 hit. [Graphical view]
TIGRFAMs	TIGR00527. gcvH. 1 hit.
PROSITE	PS00189. LIPOYL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCSH1_ARATH

Accession

Primary (citable) accession number: P25855

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	June 16, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)