P25853 (BAM5_ARATH) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 107.
History...
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Beta-amylase 5 Short name=AtBeta-Amy EC=3.2.1.2 Alternative name(s): 1,4-alpha-D-glucan maltohydrolase Protein REDUCED BETA AMYLASE 1 | ||||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) [Reference proteome] | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis![]() |
Protein attributes
| Sequence length | 498 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Beta-amylase activity. Major cytosolic beta-amylase isoform in rosette leaves and inflorescences stems. Ref.10 |
| Catalytic activity | Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains. |
| Subcellular location | Cytoplasm. Note: Present in the continuous phloem cytoplasm. Ref.9 |
| Tissue specificity | Detected in phloem sieve elements. Ref.9 |
| Induction | Circadian-regulated, with a peak in expression just before the light period in short day conditions. |
| Disruption phenotype | Almost complete loss of beta-amylase activity in rosette leaves and inflorescences (stems). Ref.10 |
| Sequence similarities | Belongs to the glycosyl hydrolase 14 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Carbohydrate metabolism Polysaccharide degradation |
| Cellular component | Cytoplasm |
| Coding sequence diversity | Alternative splicing |
| Molecular function | Glycosidase Hydrolase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | response to herbivore Inferred from expression pattern PubMed 19251652. Source: TAIR starch catabolic processInferred from direct assay Ref.2. Source: TAIR |
| Cellular_component | cytosol Inferred from direct assay PubMed 21166475. Source: TAIR |
| Molecular_function | beta-amylase activity Inferred from direct assay Ref.2. Source: TAIR |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: P25853-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: P25853-2) The sequence of this isoform differs from the canonical sequence as follows: 411-420: KPKLRMYGFT → LLWRRSEVRA 421-498: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 498 | 498 | Beta-amylase 5 | PRO_0000153932 | |||||
Regions | |||||||||
| Region | 384 – 385 | 2 | Substrate binding By similarity | ||||||
Sites | |||||||||
| Active site | 189 | 1 | Proton donor By similarity | ||||||
| Active site | 383 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 56 | 1 | Substrate By similarity | ||||||
| Binding site | 96 | 1 | Substrate By similarity | ||||||
| Binding site | 104 | 1 | Substrate By similarity | ||||||
| Binding site | 298 | 1 | Substrate By similarity | ||||||
| Binding site | 303 | 1 | Substrate By similarity | ||||||
| Binding site | 345 | 1 | Substrate By similarity | ||||||
| Binding site | 423 | 1 | Substrate By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 411 – 420 | 10 | KPKLRMYGFT → LLWRRSEVRA in isoform 2. | VSP_038978 | |||||
| Alternative sequence | 421 – 498 | 78 | Missing in isoform 2. | VSP_038979 | |||||
Experimental info | |||||||||
| Sequence conflict | 155 | 1 | Q → QV in CAB10300. Ref.3 | ||||||
| Sequence conflict | 155 | 1 | Q → QV in CAB78563. Ref.4 | ||||||
| Sequence conflict | 486 | 1 | F → L in BAA07842. Ref.2 | ||||||
| Sequence conflict | 486 | 1 | F → L in AAB34026. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Nucleotide sequence of a cDNA clone encoding a beta-amylase from Arabidopsis thaliana." Monroe J.D., Salminen M.D., Preiss J. Plant Physiol. 97:1599-1601(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). |
| [2] | "Sugar-inducible expression of a gene for beta-amylase in Arabidopsis thaliana." Mita S., Suzuki-Fujii K., Nakamura K. Plant Physiol. 107:895-904(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Landsberg erecta. |
| [3] | "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana." Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C., Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P., Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E. Chalwatzis N.Nature 391:485-488(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [4] | "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. McCombie W.R.Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [5] | The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia. |
| [6] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia. |
| [7] | "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs." Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K. DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia. |
| [8] | "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. Shinozaki K.Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia. |
| [9] | "Identification and characterization of a phloem-specific beta-amylase." Wang Q., Monroe J., Sjoelund R.D. Plant Physiol. 109:743-750(1995) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY. |
| [10] | "The ram1 mutant of Arabidopsis exhibits severely decreased beta-amylase activity." Laby R.J., Kim D., Gibson S.I. Plant Physiol. 127:1798-1807(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE. |
| [11] | "Beta-AMYLASE4, a noncatalytic protein required for starch breakdown, acts upstream of three active beta-amylases in Arabidopsis chloroplasts." Fulton D.C., Stettler M., Mettler T., Vaughan C.K., Li J., Francisco P., Gil M., Reinhold H., Eicke S., Messerli G., Dorken G., Halliday K., Smith A.M., Smith S.M., Zeeman S.C. Plant Cell 20:1040-1058(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | M73467 mRNA. Translation: AAA32737.1. D43783 Genomic DNA. Translation: BAA07842.1. S77076 Genomic DNA. Translation: AAB34026.1. Z97338 Genomic DNA. Translation: CAB10300.1. AL161540 Genomic DNA. Translation: CAB78563.1. CP002687 Genomic DNA. Translation: AEE83568.1. CP002687 Genomic DNA. Translation: AEE83569.1. AF424573 mRNA. Translation: AAL11567.1. AY142024 mRNA. Translation: AAM98288.1. AK316869 mRNA. Translation: BAH19577.1. AK226353 mRNA. Translation: BAE98501.1. |
| IPI | IPI00517770. IPI00532891. |
| PIR | S36094. B71416. |
| RefSeq | NP_567460.1. NM_117609.2. NP_849389.1. NM_179058.1. |
| UniGene | At.25187. |
3D structure databases | |
| ProteinModelPortal | P25853. |
| SMR | P25853. Positions 9-498. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | 3702.AT4G15210.1-P. |
Protein family/group databases | |
| CAZy | GH14. Glycoside Hydrolase Family 14. |
Proteomic databases | |
| PaxDb | P25853. |
| PRIDE | P25853. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblPlants | AT4G15210.1; AT4G15210.1; AT4G15210. |
| GeneID | 827185. |
| KEGG | ath:AT4G15210. |
Organism-specific databases | |
| TAIR | At4g15210. |
Phylogenomic databases | |
| eggNOG | NOG322510. |
| HOGENOM | HOG000238755. |
| InParanoid | P25853. |
| OMA | RYPSYPQ. |
| PhylomeDB | P25853. |
| ProtClustDB | PLN02801. |
Gene expression databases | |
| Genevestigator | P25853. |
Family and domain databases | |
| Gene3D | 3.20.20.80. 1 hit. |
| InterPro | IPR001554. Glyco_hydro_14. IPR018238. Glyco_hydro_14_CS. IPR001371. Glyco_hydro_14B_pln. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view] |
| Pfam | PF01373. Glyco_hydro_14. 1 hit. [Graphical view] |
| PRINTS | PR00750. BETAAMYLASE. PR00842. GLHYDLASE14B. |
| SUPFAM | SSF51445. Glyco_hydro_cat. 1 hit. |
| PROSITE | PS00506. BETA_AMYLASE_1. 1 hit. PS00679. BETA_AMYLASE_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | BAM5_ARATH | ||||||||
| Accession | Primary (citable) accession number: P25853 Secondary accession number(s): O23375, Q0WWJ7, Q3EA19 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with
