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Protein

Transcriptional regulatory protein ZraR

Gene

zraR

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system ZraS/ZraR. When activated by ZraS it acts in conjunction with sigma-54 to regulate the expression of zraP. Positively autoregulates the expression of the zraSR operon (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi169 – 1768ATPPROSITE-ProRule annotation
Nucleotide bindingi232 – 24110ATPPROSITE-ProRule annotation
DNA bindingi421 – 44020H-T-H motifBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-3616-MONOMER.
SENT99287:GCTI-4204-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein ZraR
Gene namesi
Name:zraR
Synonyms:hydG
Ordered Locus Names:STM4174
ORF Names:STMF1.27
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 441441Transcriptional regulatory protein ZraRPRO_0000081283Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei56 – 5614-aspartylphosphatePROSITE-ProRule annotation

Post-translational modificationi

Phosphorylated by ZraS.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP25852.
PRIDEiP25852.

Interactioni

Protein-protein interaction databases

STRINGi99287.STM4174.

Structurei

Secondary structure

1
441
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi146 – 15813Combined sources
Beta strandi165 – 1695Combined sources
Helixi175 – 18511Combined sources
Beta strandi189 – 1913Combined sources
Beta strandi195 – 1973Combined sources
Helixi203 – 2108Combined sources
Helixi228 – 2325Combined sources
Beta strandi235 – 2417Combined sources
Helixi247 – 25812Combined sources
Beta strandi259 – 2613Combined sources
Beta strandi276 – 2838Combined sources
Helixi285 – 2917Combined sources
Helixi296 – 3027Combined sources
Beta strandi303 – 3086Combined sources
Helixi313 – 3186Combined sources
Helixi319 – 33315Combined sources
Helixi343 – 3519Combined sources
Helixi357 – 37115Combined sources
Beta strandi374 – 3774Combined sources
Helixi379 – 3813Combined sources
Helixi384 – 3863Combined sources
Helixi402 – 41514Combined sources
Turni416 – 4194Combined sources
Helixi421 – 4288Combined sources
Helixi432 – 4387Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OJLX-ray3.00A/B/C/D/E/F141-441[»]
ProteinModelPortaliP25852.
SMRiP25852. Positions 141-441.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP25852.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 121115Response regulatoryPROSITE-ProRule annotationAdd
BLAST
Domaini141 – 370230Sigma-54 factor interactionPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 response regulatory domain.PROSITE-ProRule annotation
Contains 1 sigma-54 factor interaction domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105C1W. Bacteria.
COG2204. LUCA.
HOGENOMiHOG000058489.
KOiK07713.
OMAiVATDSRW.
OrthoDBiEOG6WHNMG.
PhylomeDBiP25852.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011006. CheY-like_superfamily.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR027417. P-loop_NTPase.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
[Graphical view]
PfamiPF02954. HTH_8. 1 hit.
PF00072. Response_reg. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
PRINTSiPR01590. HTHFIS.
SMARTiSM00382. AAA. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
PS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P25852-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRGKIDILV VDDDVSHCTI LQALLRGWGY NVALAYSGHD ALAQVREKVF
60 70 80 90 100
DLVLCDVRMA EMDGIATLKE IKALNPAIPI LIMTAFSSVE TAVEALKAGA
110 120 130 140 150
LDYLIKPLDF DRLQETLEKA LAHTRETGAE LPSASAAQFG MIGSSPAMQH
160 170 180 190 200
LLNEIAMVAP SDATVLIHGD SGTGKELVAR ALHACSARSD RPLVTLNCAA
210 220 230 240 250
LNESLLESEL FGHEKGAFTG ADKRREGRFV EADGGTLFLD EIGDISPLMQ
260 270 280 290 300
VRLLRAIQER EVQRVGSNQT ISVDVRLIAA THRDLAEEVS AGRFRQDLYY
310 320 330 340 350
RLNVVAIEMP SLRQRREDIP LLADHFLRRF AERNRKVVKG FTPQAMDLLI
360 370 380 390 400
HYDWPGNIRE LENAIERAVV LLTGEYISER ELPLAIAATP IKTEYSGEIQ
410 420 430 440
PLVDVEKEVI LAALEKTGGN KTEAARQLGI TRKTLLAKLS R
Length:441
Mass (Da):48,590
Last modified:January 23, 2002 - v2
Checksum:i5753C4E54F8C52F5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti159 – 1591A → R in AAA27149 (PubMed:1756170).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64988 mRNA. Translation: AAA27149.1.
AF170176 Genomic DNA. Translation: AAF33506.1.
AE006468 Genomic DNA. Translation: AAL23002.1.
PIRiS19606.
RefSeqiNP_463043.1. NC_003197.1.
WP_000617942.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL23002; AAL23002; STM4174.
GeneIDi1255700.
KEGGistm:STM4174.
PATRICi32387233. VBISalEnt20916_4388.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64988 mRNA. Translation: AAA27149.1.
AF170176 Genomic DNA. Translation: AAF33506.1.
AE006468 Genomic DNA. Translation: AAL23002.1.
PIRiS19606.
RefSeqiNP_463043.1. NC_003197.1.
WP_000617942.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OJLX-ray3.00A/B/C/D/E/F141-441[»]
ProteinModelPortaliP25852.
SMRiP25852. Positions 141-441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM4174.

Proteomic databases

PaxDbiP25852.
PRIDEiP25852.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL23002; AAL23002; STM4174.
GeneIDi1255700.
KEGGistm:STM4174.
PATRICi32387233. VBISalEnt20916_4388.

Phylogenomic databases

eggNOGiENOG4105C1W. Bacteria.
COG2204. LUCA.
HOGENOMiHOG000058489.
KOiK07713.
OMAiVATDSRW.
OrthoDBiEOG6WHNMG.
PhylomeDBiP25852.

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-3616-MONOMER.
SENT99287:GCTI-4204-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP25852.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011006. CheY-like_superfamily.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR027417. P-loop_NTPase.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
[Graphical view]
PfamiPF02954. HTH_8. 1 hit.
PF00072. Response_reg. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
PRINTSiPR01590. HTHFIS.
SMARTiSM00382. AAA. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
PS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequence analysis of hydrogenase regulatory genes (hydHG) from Salmonella typhimurium."
    Chopra A.K., Peterson J.W., Prasad R.
    Biochim. Biophys. Acta 1129:115-118(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.

Entry informationi

Entry nameiZRAR_SALTY
AccessioniPrimary (citable) accession number: P25852
Secondary accession number(s): Q9L9H8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: January 23, 2002
Last modified: November 11, 2015
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be involved in the regulation of the labile hydrogenase activity.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.