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Reviewed, UniProtKB/Swiss-Prot P25851 (F16P1_ARATH)

Last modified May 5, 2009. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Fructose-1,6-bisphosphatase, chloroplastic
      Short name=FBPase
    EC=3.1.3.11
Alternative name(s):
    D-fructose-1,6-bisphosphate 1-phosphohydrolase
Gene names
Name: FBP
Ordered Locus Names: At3g54050
ORF Names: F24B22.10
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length417 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.

Cofactor

Binds 3 magnesium ions per subunit By similarity.

Pathway

Carbohydrate biosynthesis; Calvin cycle.

Subunit structure

Homotetramer By similarity.

Subcellular location

Plastid › chloroplast.

Induction

Light activation through pH changes, Mg2+ levels and also by light-modulated reduction of essential disulfide groups via the ferredoxin-thioredoxin f system By similarity.

Miscellaneous

In plants there are two FBPase isozymes: one in the cytosol and the other in the chloroplast.

Sequence similarities

Belongs to the FBPase class 1 family.

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Ontologies

Keywords
   Biological processCalvin cycle
Carbohydrate metabolism
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandMagnesium
Metal-binding
   Molecular functionHydrolase
   PTMDisulfide bond
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processreductive pentose-phosphate cycle

Inferred from electronic annotation. Source: UniProtKB-KW

response to cold

Inferred from expression pattern. Source: TAIR

   Cellular componentapoplast

Inferred from direct assay. Source: TAIR

chloroplast stroma

Inferred from direct assay. Source: TAIR

   Molecular functionfructose 1,6-bisphosphate 1-phosphatase activity

Inferred from electronic annotation. Source: EC

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5959Chloroplast Potential
Chain60 – 417358Fructose-1,6-bisphosphatase, chloroplastic
PRO_0000008814

Regions

Region191 – 1944Substrate binding By similarity

Sites

Metal binding1381Magnesium 1 By similarity
Metal binding1671Magnesium 1 By similarity
Metal binding1671Magnesium 2 By similarity
Metal binding1881Magnesium 2 By similarity
Metal binding1881Magnesium 3 By similarity
Metal binding1901Magnesium 2; via carbonyl oxygen By similarity
Metal binding1911Magnesium 3 By similarity
Metal binding3651Magnesium 3 By similarity
Binding site2971Substrate By similarity
Binding site3291Substrate By similarity
Binding site3471Substrate By similarity
Binding site3491Substrate By similarity
Binding site3591Substrate By similarity

Amino acid modifications

Disulfide bond233 ↔ 238Redox-active (light-modulated) By similarity

Experimental info

Sequence conflict41T → S in CAA41154. Ref.1
Sequence conflict661A → S in CAA41154. Ref.1
Sequence conflict1331V → I in CAA41154. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P25851-1 [UniParc].

Last modified June 6, 2002. Version 2.
Checksum: CEB17F65468746D7

FASTA41745,162
        10         20         30         40         50         60 
MAATAATTTS SHLLLSSSRH VASSSQPSIL SPRSLFSNNG KRAPTGVRNH QYASGVRCMA 

        70         80         90        100        110        120 
VAADAAETKT AARKKSGYEL QTLTGWLLRQ EMKGEIDAEL TIVMSSISLA CKQIASLVQR 

       130        140        150        160        170        180 
AGISNLTGVQ GAVNIQGEDQ KKLDVISNEV FSNCLRSSGR TGIIASEEED VPVAVEESYS 

       190        200        210        220        230        240 
GNYVVVFDPL DGSSNIDAAV STGSIFGIYS PNDECIVDDS DDISALGSEE QRCIVNVCQP 

       250        260        270        280        290        300 
GNNLLAAGYC MYSSSVIFVL TLGKGVFSFT LDPMYGEFVL TQENIEIPKA GRIYSFNEGN 

       310        320        330        340        350        360 
YQMWDDKLKK YIDDLKDPGP TGKPYSARYI GSLVGDFHRT LLYGGIYGYP RDAKSKNGKL 

       370        380        390        400        410 
RLLYECAPMS FIVEQAGGKG SDGHSRVLDI QPTEIHQRVP LYIGSTEEVE KLEKYLA

« Hide

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References

« Hide 'large scale' references
[1]"Nucleotide sequence of a cDNA clone encoding chloroplast fructose-1,6-bisphosphatase from Arabidopsis thaliana."
Horsnell P.R., Raines C.A.
Plant Mol. Biol. 17:185-186(1991) [PubMed: 1651131] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed: 11130713] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.

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Cross-references

Sequence databases

X58148 mRNA. Translation: CAA41154.1.
AL132957 Genomic DNA. Translation: CAB70979.1.
AF428326 mRNA. Translation: AAL16256.1.
AY039934 mRNA. Translation: AAK64038.1.
AY150450 mRNA. Translation: AAN12891.1.
BT000743 mRNA. Translation: AAN31884.1.
IPIIPI00530130.
PIRS16582.
T47564.
RefSeqNP_190973.1.
UniGeneAt.22619

3D structure databases

HSSPHSSP built from PDB template 1DBZ based on UniProtKB P46275.
SMRP25851. Positions 78-417.
ModBaseSearch...

Proteomic databases

PRIDEP25851.
ProMEXP25851.

Genome annotation databases

GeneID824572.
GenomeReviewsGene locus AT3G54050 in contig BA000014_GR.
KEGGath:AT3G54050.
NMPDRfig|3702.1.peg.16704.

Organism-specific databases

TAIRAt3g54050.

Phylogenomic databases

OMAP25851. IDAAVST.

Enzyme and pathway databases

BRENDA3.1.3.11. 302.

Gene expression databases

ArrayExpressP25851.
GermOnlineAT3G54050. Arabidopsis thaliana.

Family and domain databases

InterProIPR000146. Fructose_bisphosphatase.
IPR017955. IMPase/FBPase.
[Graphical view]
PANTHERPTHR11556. In_FB_phphtase. 1 hit.
PfamPF00316. FBPase. 1 hit.
[Graphical view]
PRINTSPR00115. FBPHPHTASE.
PR00377. INFBPHPHTASE.
ProDomPD001491. In_FB_phphtase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS00124. FBPASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameF16P1_ARATH
AccessionPrimary (citable) accession number: P25851
Secondary accession number(s): Q9M398
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: June 6, 2002
Last modified: May 5, 2009
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents