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Protein

Fructose-1,6-bisphosphatase 1, chloroplastic

Gene

CFBP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the irreversible reaction from fructose-1,6-bisphosphate to fructose-6-phosphate and inorganic phosphate, to regenerate the primary CO2 acceptor molecule, ribulose-1,5-bisphosphate (Probable). Involved in the regulation of photosynthetic electron flow and sucrose synthesis (PubMed:15448173, PubMed:20081115). Its activity is critical for normal plant development and important for the regulation of a wide range of metabolic processes (PubMed:25743161).1 Publication3 Publications

Miscellaneous

In plants there are two FBPase isozymes: one in the cytosol and the other in the chloroplast.Curated

Catalytic activityi

D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.Curated

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Pathwayi: Calvin cycle

This protein is involved in the pathway Calvin cycle, which is part of Carbohydrate biosynthesis.Curated
View all proteins of this organism that are known to be involved in the pathway Calvin cycle and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi138Magnesium 1By similarity1
Metal bindingi167Magnesium 1By similarity1
Metal bindingi167Magnesium 2By similarity1
Metal bindingi188Magnesium 2By similarity1
Metal bindingi188Magnesium 3By similarity1
Metal bindingi190Magnesium 2; via carbonyl oxygenBy similarity1
Metal bindingi191Magnesium 3By similarity1
Binding sitei297SubstrateBy similarity1
Binding sitei329SubstrateBy similarity1
Binding sitei347SubstrateBy similarity1
Binding sitei349SubstrateBy similarity1
Binding sitei359SubstrateBy similarity1
Metal bindingi365Magnesium 3By similarity1

GO - Molecular functioni

  • fructose 1,6-bisphosphate 1-phosphatase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • fructose 1,6-bisphosphate metabolic process Source: TAIR
  • fructose 6-phosphate metabolic process Source: GO_Central
  • fructose metabolic process Source: GO_Central
  • gluconeogenesis Source: GO_Central
  • photosynthesis Source: TAIR
  • photosynthetic electron transport in photosystem I Source: TAIR
  • reductive pentose-phosphate cycle Source: UniProtKB-UniPathway
  • response to cold Source: TAIR
  • response to cytokinin Source: TAIR
  • sucrose biosynthetic process Source: GO_Central
  • sucrose metabolic process Source: TAIR

Keywordsi

Molecular functionHydrolase
Biological processCalvin cycle, Carbohydrate metabolism
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT3G54050-MONOMER.
MetaCyc:AT3G54050-WS-MONOMER.
UniPathwayiUPA00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-1,6-bisphosphatase 1, chloroplasticCurated (EC:3.1.3.11Curated)
Short name:
FBPase1Curated
Alternative name(s):
D-fructose-1,6-bisphosphate 1-phosphohydrolaseCurated
Protein HIGH CYCLIC ELECTRON FLOW 11 Publication
Gene namesi
Name:CFBP11 Publication
Synonyms:FBP1 Publication, HCEF11 Publication
Ordered Locus Names:At3g54050
ORF Names:F24B22.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G54050.
TAIRilocus:2080225. AT3G54050.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Reduced growth rate, dwarf phenotype and delayed flowering.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi361R → K in hcef1; reduced growth rate, dwarf phenotype and delayed flowering. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 59ChloroplastSequence analysisCombined sourcesAdd BLAST59
ChainiPRO_000000881460 – 417Fructose-1,6-bisphosphatase 1, chloroplasticAdd BLAST358

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei60N-acetylalanineCombined sources1
Disulfide bondi233 ↔ 238Redox-active (light-modulated)By similarity

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

PaxDbiP25851.
PRIDEiP25851.

PTM databases

iPTMnetiP25851.

Expressioni

Inductioni

Light activation through pH changes, Mg2+ levels and also by light-modulated reduction of essential disulfide groups via the ferredoxin-thioredoxin f system.By similarity

Gene expression databases

GenevisibleiP25851. AT.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi9889. 2 interactors.
IntActiP25851. 1 interactor.
STRINGi3702.AT3G54050.1.

Structurei

3D structure databases

ProteinModelPortaliP25851.
SMRiP25851.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni191 – 194Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the FBPase class 1 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1458. Eukaryota.
COG0158. LUCA.
HOGENOMiHOG000191265.
InParanoidiP25851.
KOiK03841.
OMAiCMYSSST.
OrthoDBiEOG09360D1I.
PhylomeDBiP25851.

Family and domain databases

CDDicd00354. FBPase. 1 hit.
HAMAPiMF_01855. FBPase_class1. 1 hit.
InterProiView protein in InterPro
IPR000146. FBPase_class-1.
IPR033391. FBPase_N.
IPR028343. FBPtase.
IPR020548. Fructose_bisphosphatase_AS.
PANTHERiPTHR11556. PTHR11556. 1 hit.
PfamiView protein in Pfam
PF00316. FBPase. 1 hit.
PIRSFiPIRSF500210. FBPtase. 1 hit.
PIRSF000904. FBPtase_SBPase. 1 hit.
PRINTSiPR00115. F16BPHPHTASE.
PROSITEiView protein in PROSITE
PS00124. FBPASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25851-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATAATTTS SHLLLSSSRH VASSSQPSIL SPRSLFSNNG KRAPTGVRNH
60 70 80 90 100
QYASGVRCMA VAADAAETKT AARKKSGYEL QTLTGWLLRQ EMKGEIDAEL
110 120 130 140 150
TIVMSSISLA CKQIASLVQR AGISNLTGVQ GAVNIQGEDQ KKLDVISNEV
160 170 180 190 200
FSNCLRSSGR TGIIASEEED VPVAVEESYS GNYVVVFDPL DGSSNIDAAV
210 220 230 240 250
STGSIFGIYS PNDECIVDDS DDISALGSEE QRCIVNVCQP GNNLLAAGYC
260 270 280 290 300
MYSSSVIFVL TLGKGVFSFT LDPMYGEFVL TQENIEIPKA GRIYSFNEGN
310 320 330 340 350
YQMWDDKLKK YIDDLKDPGP TGKPYSARYI GSLVGDFHRT LLYGGIYGYP
360 370 380 390 400
RDAKSKNGKL RLLYECAPMS FIVEQAGGKG SDGHSRVLDI QPTEIHQRVP
410
LYIGSTEEVE KLEKYLA
Length:417
Mass (Da):45,162
Last modified:June 6, 2002 - v2
Checksum:iCEB17F65468746D7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4T → S in CAA41154 (PubMed:1651131).Curated1
Sequence conflicti66A → S in CAA41154 (PubMed:1651131).Curated1
Sequence conflicti133V → I in CAA41154 (PubMed:1651131).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X58148 mRNA. Translation: CAA41154.1.
AL132957 Genomic DNA. Translation: CAB70979.1.
CP002686 Genomic DNA. Translation: AEE79179.1.
CP002686 Genomic DNA. Translation: AEE79180.1.
AF428326 mRNA. Translation: AAL16256.1.
AY039934 mRNA. Translation: AAK64038.1.
AY150450 mRNA. Translation: AAN12891.1.
BT000743 mRNA. Translation: AAN31884.1.
PIRiS16582.
T47564.
RefSeqiNP_001190083.1. NM_001203154.1.
NP_190973.1. NM_115265.5.
UniGeneiAt.22619.
At.51003.

Genome annotation databases

EnsemblPlantsiAT3G54050.1; AT3G54050.1; AT3G54050.
AT3G54050.2; AT3G54050.2; AT3G54050.
GeneIDi824572.
GrameneiAT3G54050.1; AT3G54050.1; AT3G54050.
AT3G54050.2; AT3G54050.2; AT3G54050.
KEGGiath:AT3G54050.

Similar proteinsi

Entry informationi

Entry nameiF16P1_ARATH
AccessioniPrimary (citable) accession number: P25851
Secondary accession number(s): Q9M398
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: June 6, 2002
Last modified: October 25, 2017
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families