##gff-version 3
P25847	UniProtKB	Chain	1	964	.	.	.	ID=PRO_0000115191;Note=DNA mismatch repair protein MSH2	
P25847	UniProtKB	Region	851	964	.	.	.	Note=Interaction with MSH6	
P25847	UniProtKB	Binding site	688	695	.	.	.	Ontology_term=ECO:0000255;evidence=ECO:0000255	
P25847	UniProtKB	Mutagenesis	42	42	.	.	.	Note=Moderately reduced activity in a mismatch repair assay. Y->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11641390;Dbxref=PMID:11641390	
P25847	UniProtKB	Mutagenesis	65	65	.	.	.	Note=Defective in a mismatch repair assay. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11641390;Dbxref=PMID:11641390	
P25847	UniProtKB	Mutagenesis	317	317	.	.	.	Note=Partially defective in a mismatch repair assay. G->D;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:10469597,ECO:0000269|PubMed:11555625;Dbxref=PMID:10469597,PMID:11555625	
P25847	UniProtKB	Mutagenesis	402	402	.	.	.	Note=Partially defective in a mismatch repair assay. L->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10469597;Dbxref=PMID:10469597	
P25847	UniProtKB	Mutagenesis	430	430	.	.	.	Note=Partially defective in a mismatch repair assay. Q->K;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10469597;Dbxref=PMID:10469597	
P25847	UniProtKB	Mutagenesis	518	518	.	.	.	Note=Defective in MMR%2C but not in NHTR. A->P;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10523644;Dbxref=PMID:10523644	
P25847	UniProtKB	Mutagenesis	524	524	.	.	.	Note=Partially defective in a mismatch repair assay. D->Y;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10469597;Dbxref=PMID:10469597	
P25847	UniProtKB	Mutagenesis	542	542	.	.	.	Note=Defective in a mismatch repair assay. R->P;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10469597;Dbxref=PMID:10469597	
P25847	UniProtKB	Mutagenesis	561	561	.	.	.	Note=Causes strong defects in MutS alpha mismatch binding. Defective in MMR%2C but not in NHTR. S->P;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12875840;Dbxref=PMID:12875840	
P25847	UniProtKB	Mutagenesis	564	564	.	.	.	Note=Causes strong defects in MutS alpha mismatch binding. Defective in MMR%2C but not in NHTR. K->E;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12875840;Dbxref=PMID:12875840	
P25847	UniProtKB	Mutagenesis	566	566	.	.	.	Note=Defective in MMR%2C but not in NHTR. G->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12875840;Dbxref=PMID:12875840	
P25847	UniProtKB	Mutagenesis	574	574	.	.	.	Note=Defective in MMR and in NHTR. L->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10523644;Dbxref=PMID:10523644	
P25847	UniProtKB	Mutagenesis	584	584	.	.	.	Note=Defective in MMR and in NHTR. L->P;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10523644;Dbxref=PMID:10523644	
P25847	UniProtKB	Mutagenesis	640	640	.	.	.	Note=Defective in a mismatch repair assay. P->L;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:10469597,ECO:0000269|PubMed:11555625;Dbxref=PMID:10469597,PMID:11555625	
P25847	UniProtKB	Mutagenesis	656	656	.	.	.	Note=Causes defects in ATP-dependent dissociation of MutS alpha from mismatch DNA and in interactions between MutS alpha and MutL alpha. Defective in MMR%2C but not in NHTR. S->P;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12875840;Dbxref=PMID:12875840	
P25847	UniProtKB	Mutagenesis	658	658	.	.	.	Note=Fully functional in a mismatch repair assay. H->Y;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11555625;Dbxref=PMID:11555625	
P25847	UniProtKB	Mutagenesis	688	688	.	.	.	Note=Moderately reduced activity in a mismatch repair assay. G->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10077621;Dbxref=PMID:10077621	
P25847	UniProtKB	Mutagenesis	693	693	.	.	.	Note=Has a dominant negative mutator effect. G->A%2CS;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:10077621,ECO:0000269|PubMed:9819445;Dbxref=PMID:10077621,PMID:9819445	
P25847	UniProtKB	Mutagenesis	693	693	.	.	.	Note=Has no defect in mismatch DNA binding%2C but lacks ATP-induced conformational change. Defective in MMR and in NHTR. G->D;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:10077621,ECO:0000269|PubMed:9819445;Dbxref=PMID:10077621,PMID:9819445	
P25847	UniProtKB	Mutagenesis	694	694	.	.	.	Note=Impairs ATP binding%3B reduces catalytic activity 1.6-fold for ATP hydrolysis. Has a dominant negative mutator effect. K->A;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:10077621,ECO:0000269|PubMed:16214425,ECO:0000269|PubMed:16600868;Dbxref=PMID:10077621,PMID:16214425,PMID:16600868	
P25847	UniProtKB	Mutagenesis	694	694	.	.	.	Note=Defective in MMR and in NHTR. K->R;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:10077621,ECO:0000269|PubMed:16214425,ECO:0000269|PubMed:16600868;Dbxref=PMID:10077621,PMID:16214425,PMID:16600868	
P25847	UniProtKB	Mutagenesis	695	695	.	.	.	Note=Has a dominant negative mutator effect. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10077621;Dbxref=PMID:10077621	
P25847	UniProtKB	Mutagenesis	716	716	.	.	.	Note=Defective in a mismatch repair assay. C->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10469597;Dbxref=PMID:10469597	
P25847	UniProtKB	Mutagenesis	730	730	.	.	.	Note=Disrupts MutS alpha ATPase activity%2C but does not affect ATP binding or interactions with MutL alpha. Defective in MMR%2C but not in NHTR. R->W;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12875840;Dbxref=PMID:12875840	
P25847	UniProtKB	Mutagenesis	742	742	.	.	.	Note=Defective in MMR%2C but not in NHTR. S->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10523644;Dbxref=PMID:10523644	
P25847	UniProtKB	Mutagenesis	742	742	.	.	.	Note=Defective in MMR and in NHTR. S->P;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10523644;Dbxref=PMID:10523644	
P25847	UniProtKB	Mutagenesis	768	768	.	.	.	Note=Reduces catalytic activity 50-fold for ATP hydrolysis. E->A;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:12509278,ECO:0000269|PubMed:15513922,ECO:0000269|PubMed:16214425;Dbxref=PMID:12509278,PMID:15513922,PMID:16214425	
P25847	UniProtKB	Mutagenesis	773	773	.	.	.	Note=Defective in MMR and in NHTR. T->I;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10523644;Dbxref=PMID:10523644	
P25847	UniProtKB	Mutagenesis	855	855	.	.	.	Note=Defective in MMR%2C but not in NHTR. G->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10523644;Dbxref=PMID:10523644	
P25847	UniProtKB	Mutagenesis	859	859	.	.	.	Note=Defective in MMR%2C but not in NHTR. A->E;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10523644;Dbxref=PMID:10523644	
P25847	UniProtKB	Mutagenesis	862	862	.	.	.	Note=Defective in MMR%2C but not in NHTR. V->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10523644;Dbxref=PMID:10523644	
P25847	UniProtKB	Mutagenesis	863	868	.	.	.	Note=Defective in MMR and in NHTR. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10523644;Dbxref=PMID:10523644	
P25847	UniProtKB	Sequence conflict	957	964	.	.	.	Note=KYIKALLL->EIYKSPCCYN;Ontology_term=ECO:0000305;evidence=ECO:0000305