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Protein

DNA mismatch repair protein MSH1, mitochondrial

Gene

MSH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Important for mitochondrial DNA (mtDNA) stability and repair. Recognizes and binds to base-base and small insertion-deletion mismatches in mtDNA. ATP binding and hydrolysis is crucial for function. Binding to a mismatched base pair attenuates ATP hydrolysis. Also involved in proper sorting of mtDNA during mtDNA transmission.7 Publications

Miscellaneous

Present with 1670 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi771 – 778ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • damaged DNA binding Source: GO_Central
  • dinucleotide insertion or deletion binding Source: SGD
  • DNA-dependent ATPase activity Source: SGD
  • double-strand/single-strand DNA junction binding Source: GO_Central
  • guanine/thymine mispair binding Source: SGD
  • Y-form DNA binding Source: GO_Central

GO - Biological processi

  • DNA repair Source: SGD
  • mismatch repair Source: SGD
  • mitochondrial DNA repair Source: SGD
  • mitochondrial genome maintenance Source: SGD
  • mitochondrion organization Source: SGD

Keywordsi

Molecular functionDNA-binding
Biological processDNA damage, DNA repair
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31162-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA mismatch repair protein MSH1, mitochondrial
Alternative name(s):
MutS protein homolog 1
Gene namesi
Name:MSH1
Ordered Locus Names:YHR120W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR120W
SGDiS000001162 MSH1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi105F → A: Increases the rate of point mutations 35-fold and displays a significant decrease in respiration-competent cells during a time course. 2 Publications1
Mutagenesisi776G → D: Increases the rate of point mutations 19-fold and displays a moderate decrease in respiration-competent cells during a time course. 3 Publications1
Mutagenesisi813R → W: Slightly increases the frequency of point mutations and repeat-mediated deletion rates in mtDNA. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 21Mitochondrion1 PublicationAdd BLAST21
ChainiPRO_000011517922 – 959DNA mismatch repair protein MSH1, mitochondrialAdd BLAST938

Proteomic databases

MaxQBiP25846
PaxDbiP25846
PRIDEiP25846

PTM databases

iPTMnetiP25846

Interactioni

Subunit structurei

Homodimer.Curated

Protein-protein interaction databases

BioGridi36553, 139 interactors
DIPiDIP-6433N
IntActiP25846, 12 interactors
STRINGi4932.YHR120W

Structurei

3D structure databases

ProteinModelPortaliP25846
SMRiP25846
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA mismatch repair MutS family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000157750
InParanoidiP25846
OMAiAGFPFFQ
OrthoDBiEOG092C0EWT

Family and domain databases

Gene3Di3.30.420.110, 1 hit
3.40.1170.10, 1 hit
InterProiView protein in InterPro
IPR007695 DNA_mismatch_repair_MutS-lik_N
IPR017261 DNA_mismatch_repair_MutS/MSH
IPR000432 DNA_mismatch_repair_MutS_C
IPR007696 DNA_mismatch_repair_MutS_core
IPR016151 DNA_mismatch_repair_MutS_N
IPR036187 DNA_mismatch_repair_MutS_sf
IPR007860 DNA_mmatch_repair_MutS_con_dom
IPR036678 MutS_con_dom_sf
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF01624 MutS_I, 1 hit
PF05188 MutS_II, 1 hit
PF05192 MutS_III, 1 hit
PF00488 MutS_V, 1 hit
PIRSFiPIRSF037677 DNA_mis_repair_Msh6, 1 hit
SMARTiView protein in SMART
SM00534 MUTSac, 1 hit
SM00533 MUTSd, 1 hit
SUPFAMiSSF48334 SSF48334, 2 hits
SSF52540 SSF52540, 1 hit
SSF53150 SSF53150, 2 hits
SSF55271 SSF55271, 1 hit
PROSITEiView protein in PROSITE
PS00486 DNA_MISMATCH_REPAIR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25846-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHFFRLPTA FRPISRVSLR YSSTDTAQPK ISKLKISFNK ISESNSEKKD
60 70 80 90 100
NLGSIDTRNC LSTQQDDKLS STEPSKASLP PSLQYVRDLM DLYKDHVVLT
110 120 130 140 150
QMGSFYELYF EQAIRYAPEL NISLTNRAYS HGKVPFAGFP VHQLSRHLKM
160 170 180 190 200
LVNNCGYSVT IAEQFKKKDV ADNEANKFYR RVTRIVTPGT FIDEAFENLR
210 220 230 240 250
ENTYLLNIEF PENCMSQVAD TSLKVGICWC DVSTGEIFVQ QVYLRDLVSA
260 270 280 290 300
ITRIQPKEIL LDERLLEFHI ESGTWYPELV ELKKFFIKYQ KMPSQHRTIE
310 320 330 340 350
SFYGLFNLGG KEATERQLKI QFQTFTQKEL AALRNTLIYV SNHLPDFSIN
360 370 380 390 400
FQIPQRQLAT AIMQIDSRTS TALELHSTVR DNNKKGSLLS SIRRTVTPSG
410 420 430 440 450
TRLLSQWLSG PSLDLKEIKK RQKIVAFFKD NRDITETLRT MLKKVNDLSR
460 470 480 490 500
ILQKFSFGRG EALELIQMAR SLEVSREIRK YLLNNTSLMK ATLKSQITQL
510 520 530 540 550
TESLNFEKNL IDDILKFLNE EELAKSQDAK QNADVTRMLD IDVKDKKESN
560 570 580 590 600
KDEIFELRDF IVNPSFNTKL RKLHDTYQGV WQKKTEYNAL LKGFFVGDLG
610 620 630 640 650
AKTFTLKERQ NGEYALHVTG TASSLKKIDE LISKSTEYHG SCFHILQKSS
660 670 680 690 700
QTRWLSHKIW TDLGHELELL NLKIRNEEAN IIDLFKRKFI DRSNEVRQVA
710 720 730 740 750
TTLGYLDTLS SFAVLANERN LVCPKVDESN KLEVVNGRHL MVEEGLSARS
760 770 780 790 800
LETFTANNCE LAKDNLWVIT GPNMGGKSTF LRQNAIIVIL AQIGCFVPCS
810 820 830 840 850
KARVGIVDKL FSRVGSADDL YNEMSTFMVE MIETSFILQG ATERSLAILD
860 870 880 890 900
EIGRGTSGKE GISIAYATLK YLLENNQCRT LFATHFGQEL KQIIDNKCSK
910 920 930 940 950
GMSEKVKFYQ SGITDLGGNN FCYNHKLKPG ICTKSDAIRV AELAGFPMEA

LKEAREILG
Length:959
Mass (Da):109,409
Last modified:February 1, 1995 - v2
Checksum:iF40985CB4D2ECFF1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti437T → N in AAA34801 (PubMed:1459447).Curated1
Sequence conflicti695E → V in AAA34801 (PubMed:1459447).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84169 Genomic DNA Translation: AAA34801.1
U00059 Genomic DNA Translation: AAB68849.1
BK006934 Genomic DNA Translation: DAA06814.1
PIRiS48962
RefSeqiNP_011988.1, NM_001179250.1

Genome annotation databases

EnsemblFungiiYHR120W; YHR120W; YHR120W
GeneIDi856520
KEGGisce:YHR120W

Similar proteinsi

Entry informationi

Entry nameiMSH1_YEAST
AccessioniPrimary (citable) accession number: P25846
Secondary accession number(s): D3DL70
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

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