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Protein

DNA mismatch repair protein MSH1, mitochondrial

Gene

MSH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Important for mitochondrial DNA (mtDNA) stability and repair. Recognizes and binds to base-base and small insertion-deletion mismatches in mtDNA. ATP binding and hydrolysis is crucial for function. Binding to a mismatched base pair attenuates ATP hydrolysis. Also involved in proper sorting of mtDNA during mtDNA transmission.7 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi771 – 7788ATPSequence analysis

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • dinucleotide insertion or deletion binding Source: SGD
  • DNA-dependent ATPase activity Source: SGD
  • guanine/thymine mispair binding Source: SGD

GO - Biological processi

  • DNA repair Source: SGD
  • mismatch repair Source: SGD
  • mitochondrial DNA repair Source: SGD
  • mitochondrial genome maintenance Source: SGD
  • mitochondrion organization Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31162-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA mismatch repair protein MSH1, mitochondrial
Alternative name(s):
MutS protein homolog 1
Gene namesi
Name:MSH1
Ordered Locus Names:YHR120W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR120W.
SGDiS000001162. MSH1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi105 – 1051F → A: Increases the rate of point mutations 35-fold and displays a significant decrease in respiration-competent cells during a time course. 2 Publications
Mutagenesisi776 – 7761G → D: Increases the rate of point mutations 19-fold and displays a moderate decrease in respiration-competent cells during a time course. 3 Publications
Mutagenesisi813 – 8131R → W: Slightly increases the frequency of point mutations and repeat-mediated deletion rates in mtDNA. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2121Mitochondrion1 PublicationAdd
BLAST
Chaini22 – 959938DNA mismatch repair protein MSH1, mitochondrialPRO_0000115179Add
BLAST

Proteomic databases

MaxQBiP25846.

PTM databases

iPTMnetiP25846.

Interactioni

Subunit structurei

Homodimer.Curated

Protein-protein interaction databases

BioGridi36553. 50 interactions.
DIPiDIP-6433N.
IntActiP25846. 2 interactions.
MINTiMINT-683422.

Structurei

3D structure databases

ProteinModelPortaliP25846.
SMRiP25846. Positions 69-958.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA mismatch repair MutS family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000157750.
InParanoidiP25846.
OMAiRVGGFYE.
OrthoDBiEOG092C0EWT.

Family and domain databases

Gene3Di3.30.420.110. 1 hit.
3.40.1170.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR007695. DNA_mismatch_repair_MutS-lik_N.
IPR000432. DNA_mismatch_repair_MutS_C.
IPR007696. DNA_mismatch_repair_MutS_core.
IPR016151. DNA_mismatch_repair_MutS_N.
IPR007860. DNA_mmatch_repair_MutS_con_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01624. MutS_I. 1 hit.
PF05188. MutS_II. 1 hit.
PF05192. MutS_III. 1 hit.
PF00488. MutS_V. 1 hit.
[Graphical view]
SMARTiSM00534. MUTSac. 1 hit.
SM00533. MUTSd. 1 hit.
[Graphical view]
SUPFAMiSSF48334. SSF48334. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF53150. SSF53150. 2 hits.
SSF55271. SSF55271. 1 hit.
PROSITEiPS00486. DNA_MISMATCH_REPAIR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25846-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHFFRLPTA FRPISRVSLR YSSTDTAQPK ISKLKISFNK ISESNSEKKD
60 70 80 90 100
NLGSIDTRNC LSTQQDDKLS STEPSKASLP PSLQYVRDLM DLYKDHVVLT
110 120 130 140 150
QMGSFYELYF EQAIRYAPEL NISLTNRAYS HGKVPFAGFP VHQLSRHLKM
160 170 180 190 200
LVNNCGYSVT IAEQFKKKDV ADNEANKFYR RVTRIVTPGT FIDEAFENLR
210 220 230 240 250
ENTYLLNIEF PENCMSQVAD TSLKVGICWC DVSTGEIFVQ QVYLRDLVSA
260 270 280 290 300
ITRIQPKEIL LDERLLEFHI ESGTWYPELV ELKKFFIKYQ KMPSQHRTIE
310 320 330 340 350
SFYGLFNLGG KEATERQLKI QFQTFTQKEL AALRNTLIYV SNHLPDFSIN
360 370 380 390 400
FQIPQRQLAT AIMQIDSRTS TALELHSTVR DNNKKGSLLS SIRRTVTPSG
410 420 430 440 450
TRLLSQWLSG PSLDLKEIKK RQKIVAFFKD NRDITETLRT MLKKVNDLSR
460 470 480 490 500
ILQKFSFGRG EALELIQMAR SLEVSREIRK YLLNNTSLMK ATLKSQITQL
510 520 530 540 550
TESLNFEKNL IDDILKFLNE EELAKSQDAK QNADVTRMLD IDVKDKKESN
560 570 580 590 600
KDEIFELRDF IVNPSFNTKL RKLHDTYQGV WQKKTEYNAL LKGFFVGDLG
610 620 630 640 650
AKTFTLKERQ NGEYALHVTG TASSLKKIDE LISKSTEYHG SCFHILQKSS
660 670 680 690 700
QTRWLSHKIW TDLGHELELL NLKIRNEEAN IIDLFKRKFI DRSNEVRQVA
710 720 730 740 750
TTLGYLDTLS SFAVLANERN LVCPKVDESN KLEVVNGRHL MVEEGLSARS
760 770 780 790 800
LETFTANNCE LAKDNLWVIT GPNMGGKSTF LRQNAIIVIL AQIGCFVPCS
810 820 830 840 850
KARVGIVDKL FSRVGSADDL YNEMSTFMVE MIETSFILQG ATERSLAILD
860 870 880 890 900
EIGRGTSGKE GISIAYATLK YLLENNQCRT LFATHFGQEL KQIIDNKCSK
910 920 930 940 950
GMSEKVKFYQ SGITDLGGNN FCYNHKLKPG ICTKSDAIRV AELAGFPMEA

LKEAREILG
Length:959
Mass (Da):109,409
Last modified:February 1, 1995 - v2
Checksum:iF40985CB4D2ECFF1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti437 – 4371T → N in AAA34801 (PubMed:1459447).Curated
Sequence conflicti695 – 6951E → V in AAA34801 (PubMed:1459447).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84169 Genomic DNA. Translation: AAA34801.1.
U00059 Genomic DNA. Translation: AAB68849.1.
BK006934 Genomic DNA. Translation: DAA06814.1.
PIRiS48962.
RefSeqiNP_011988.1. NM_001179250.1.

Genome annotation databases

EnsemblFungiiYHR120W; YHR120W; YHR120W.
GeneIDi856520.
KEGGisce:YHR120W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84169 Genomic DNA. Translation: AAA34801.1.
U00059 Genomic DNA. Translation: AAB68849.1.
BK006934 Genomic DNA. Translation: DAA06814.1.
PIRiS48962.
RefSeqiNP_011988.1. NM_001179250.1.

3D structure databases

ProteinModelPortaliP25846.
SMRiP25846. Positions 69-958.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36553. 50 interactions.
DIPiDIP-6433N.
IntActiP25846. 2 interactions.
MINTiMINT-683422.

PTM databases

iPTMnetiP25846.

Proteomic databases

MaxQBiP25846.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR120W; YHR120W; YHR120W.
GeneIDi856520.
KEGGisce:YHR120W.

Organism-specific databases

EuPathDBiFungiDB:YHR120W.
SGDiS000001162. MSH1.

Phylogenomic databases

HOGENOMiHOG000157750.
InParanoidiP25846.
OMAiRVGGFYE.
OrthoDBiEOG092C0EWT.

Enzyme and pathway databases

BioCyciYEAST:G3O-31162-MONOMER.

Miscellaneous databases

PROiP25846.

Family and domain databases

Gene3Di3.30.420.110. 1 hit.
3.40.1170.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR007695. DNA_mismatch_repair_MutS-lik_N.
IPR000432. DNA_mismatch_repair_MutS_C.
IPR007696. DNA_mismatch_repair_MutS_core.
IPR016151. DNA_mismatch_repair_MutS_N.
IPR007860. DNA_mmatch_repair_MutS_con_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01624. MutS_I. 1 hit.
PF05188. MutS_II. 1 hit.
PF05192. MutS_III. 1 hit.
PF00488. MutS_V. 1 hit.
[Graphical view]
SMARTiSM00534. MUTSac. 1 hit.
SM00533. MUTSd. 1 hit.
[Graphical view]
SUPFAMiSSF48334. SSF48334. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF53150. SSF53150. 2 hits.
SSF55271. SSF55271. 1 hit.
PROSITEiPS00486. DNA_MISMATCH_REPAIR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMSH1_YEAST
AccessioniPrimary (citable) accession number: P25846
Secondary accession number(s): D3DL70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: February 1, 1995
Last modified: September 7, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1670 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.