P25823 (TUD_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Maternal protein tudor

Gene names

Name:	tud
ORF Names:	CG9450

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	2515 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required during oogenesis for the formation of primordial germ cells and for normal abdominal segmentation.
Subunit structure	Interacts with vls. Ref.4
Developmental stage	Expressed throughout the life cycle.
Miscellaneous	The TUD mRNA accumulates within the posterior region of the developing oocyte during the early to middle stages of oogenesis.
Sequence similarities	Contains 9 Tudor domains.

Ontologies

Keywords
Biological process	Differentiation Oogenesis
Domain	Repeat
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	P granule organization Inferred from mutant phenotype. Source: FlyBase intracellular mRNA localization Traceable author statement. Source: FlyBase mitochondrial rRNA export from mitochondrion Inferred from mutant phenotype. Source: FlyBase pole cell development Inferred from mutant phenotype. Source: FlyBase
Cellular component	P granule Inferred from direct assay. Source: FlyBase mitochondrion Inferred from direct assay. Source: FlyBase nucleus Inferred from direct assay. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 2515

2515

Maternal protein tudor

PRO_0000183160

Regions

Domain

455 – 513

59

Tudor 1

Domain

641 – 696

56

Tudor 2

Domain

1062 – 1122

61

Tudor 3

Domain

1355 – 1414

60

Tudor 4

Domain

1662 – 1718

57

Tudor 5

Domain

1839 – 1898

60

Tudor 6

Domain

2023 – 2082

60

Tudor 7

Domain

2211 – 2269

59

Tudor 8

Domain

2392 – 2451

60

Tudor 9

Amino acid modifications

Modified residue

226

1

Phosphoserine Ref.5

Modified residue

235

1

Phosphoserine Ref.5

Modified residue

239

1

Phosphoserine Ref.5

Modified residue

800

1

Phosphoserine Ref.5

Experimental info

Sequence conflict

116

1

K → N in CAA44286. Ref.1

Sequence conflict

635 – 638

4

NQRV → TREF in CAA44286. Ref.1

Sequence conflict

1763

1

Y → I in CAA44286. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

2515

Helix Strand Turn

Details...

Beta strand

2349 – 2356

8

Beta strand

2361 – 2366

6

Helix

2367 – 2369

3

Helix

2370 – 2383

14

Helix

2384 – 2386

3

Beta strand

2398 – 2402

5

Turn

2404 – 2406

3

Beta strand

2409 – 2417

9

Beta strand

2423 – 2426

4

Beta strand

2433 – 2437

5

Helix

2444 – 2447

4

Beta strand

2452 – 2458

7

Helix

2460 – 2462

3

Helix

2465 – 2476

12

Turn

2477 – 2480

4

Beta strand

2481 – 2490

10

Beta strand

2496 – 2502

7

Helix

2507 – 2510

4

Sequences

	Sequence		Length	Mass (Da)
	P25823 [UniParc]. Last modified August 30, 2005. Version 2. Checksum: 1218700174D66701	FASTA	2,515	285,267
	10 20 30 40 50 60 MNGQARIALP SKVDLYITHV DHVGPYLKVY GHVNRDAASL ISERIRNLLP TCFAIEPSWS 70 80 90 100 110 120 VERQQALLIP GTFCIFKNIN GPAPGDVEYR RIRVVSADLE GQSMRAEIDF VDFGYKRTVD 130 140 150 160 170 180 SHDLMFPKQP KLLQNIPLHC FQYIVLGICS EWDQTDLAEV RRLVVNQIVK ITVEPTQICD 190 200 210 220 230 240 QKFASLRWKD FELNEFLVQQ KQIGVSVDKQ LMMDHCKKLW KDNPQSPVTE YNNNSIHNSK 250 260 270 280 290 300 TPMEIAREQL AVRQSLAARL DAQRSVQVTP SRPLNADAPD YTPKHLPLVN VTNVQVQMPG 310 320 330 340 350 360 LNNTQKPVFV STNPYNRANY QPAVPAAQPY VPKANPRSQY TYYNVRMNKP INAMPPPAGP 370 380 390 400 410 420 HVPIQHFNQQ ANNVSLSYVP ARFTPPPTPS IAQHQIPIPA FRTTSLTVGL TYDVVISYVE 430 440 450 460 470 480 NGPYLFWVHL KSSDHDLSTM MGQIERTKLK ALAQAPELGT ACVARFSEDG HLYRAMVCAV 490 500 510 520 530 540 YAQRYRVVYV DYGNSELLSA SDLFQIPPEL LEIKPFAFRF ALAGTKEIEP IDDSMKRIFK 550 560 570 580 590 600 KSAIYRNFEL TVQAPESVGS MQTCHLNQNG TNMLELLRQL KNSRQSYKKA EQLENDDAVE 610 620 630 640 650 660 IRFIDSPSNF YVQKVANIGK FEQLMDEMFS YYNANQRVPD QLILGAPCIV KCDQEWYRAE 670 680 690 700 710 720 ILRVDDSVIV RHVDFGYEQN VKRHLIGHIA EKHLEMPRQA IKCCLKGFEN SELSEDKITD 730 740 750 760 770 780 QFEMLAEESN IRRRTFSVRI FRIEPDGLNV VNLLAKNLNV MKKLYKLSMP FEQYLSLEKG 790 800 810 820 830 840 QFNANNTRAE SVISSELNKS HILNSTSIGE TENRLQEQEK EQQQKKVDVR QQQLAVEIPQ 850 860 870 880 890 900 AVKSVSGSKN STDWDKRSST SAGSKDSKRQ QQQQIQRIDR HLDFSCETQS TGSYSSGMSS 910 920 930 940 950 960 PRKGNRQQNG RTPIQSPRHN EKQEAKKNAR FSNSESPRRS RDGQQGNQRS QNAPQGYAQK 970 980 990 1000 1010 1020 PQRQKSTLDG NISSKRSSGV GSDIASSSSE SVAAAKPEKY VSLDKPYALQ EMKTPSKEAA 1030 1040 1050 1060 1070 1080 SLSWWLSPFQ FYIVPKSVSA KYDNIMRDMR EFYRQKQHQP LQLKVGSTVV VRQRKDNAIL 1090 1100 1110 1120 1130 1140 RATVTACNHM MRKYRVFCVD TGSLITVTSE DIWQLEQRFA DPPCMAHRCS FHSVVTNYDP 1150 1160 1170 1180 1190 1200 LYIVDRMETF VPVNAKVDCE FVSKEKSNQG SNTSSTCSYT VNIFVNGASL RDMLVKAEFL 1210 1220 1230 1240 1250 1260 TEVAPEIRVN LLAGQQIRGK FTSIRDMTSF KVQFDYGNNV NFLCTYDDAK FVKSNPNLAR 1270 1280 1290 1300 1310 1320 RFKEFYEGKS FALNVKNVCE NNIVHLRPVM PLFMEDRRSF ICPYPVVLSS FQALVVYTAK 1330 1340 1350 1360 1370 1380 PYRVYVQPQA IVPSMQTLLD NMYEHYKAKG DSLKKFDVGQ ICAVRSSDGN WYRARISGKD 1390 1400 1410 1420 1430 1440 SNAACFEVFY IDYGNTEEIK RDDIKALDAK FYEHASGFAV EINLPIGRPS NDTKLKARIS 1450 1460 1470 1480 1490 1500 EILEEKVVTI KSIEVRRSHL IADVILENNQ SVIDLLKAEK LVPGKDLDYM RKQMEKGKSR 1510 1520 1530 1540 1550 1560 TYEYIETVDL TLDEEEDKGR KETVSKSGSA NASPKKKQHN DKDREPKKSK PAEPARTVAP 1570 1580 1590 1600 1610 1620 QPVALKTPSP VPAEPAPVPK PATPVPEVVE VPEINPTVRE AAAESKQAPA QEDPYKDLDC 1630 1640 1650 1660 1670 1680 VVLSHCDNPA QFYVHPIDQL SKLNQLHENL QIVSPSLPQL MNVVNGADCV SMYSVDKCWY 1690 1700 1710 1720 1730 1740 RAKIIDAELM VLLFIDYGNT DCVSDATDIK ESMWSHIEPF CLPCALPIRP KGTADWVDAA 1750 1760 1770 1780 1790 1800 NGIFNESYSK VPRLEYLTQG DHYTTSYVNM YIDGEDVAKK LIADGFARPL EYLASGCSCY 1810 1820 1830 1840 1850 1860 ISHVNGICDF FIQLERDSKA LELIELYLRK KDTLKPLEGF EKGLIVAALF EDDELWYRAQ 1870 1880 1890 1900 1910 1920 LQKELPDSRY EVLFIDYGNT STTSKCLMLS EEIASLPSLS KKCSLQLPDA YISWSPEAEA 1930 1940 1950 1960 1970 1980 KFAELTGEGE LVFTTQLLKP GQDHVTIDLL LDGENIIDRL LPLCQRKEPK EASKESLAVT 1990 2000 2010 2020 2030 2040 TKAIITHVEN TSRIYLQFSE KDSLMDIICE KLNGSKLQPK TEKAAVDDMC VVQFADDLEF 2050 2060 2070 2080 2090 2100 YRSRILEVLE DDQYKVILID YGNTTVVDKL YELPQEFTLI KPVAEICSME PSAIFEKNKA 2110 2120 2130 2140 2150 2160 LTLTTFDALL DSCKGVVAVE FVNKSASPPV VRLTTKDKRS LKIYEHLQKL VQAELKLIQK 2170 2180 2190 2200 2210 2220 RNENSECIIS YGNSPKSFYV QMKHNSADLD LIVKTLQSLK KEKLKKLIDP TTNSNGVCYS 2230 2240 2250 2260 2270 2280 QEDACYYRCS IKSVLDPSQG FEVFLLDYGN TLVVPEVWQL PQEIEPIPSL ALHCQLSKIP 2290 2300 2310 2320 2330 2340 MDVSDEKLEE AFAALLEQHF GELYEITTQP NEDETKPLIA ELRINYKDFV QELVSTVTGV 2350 2360 2370 2380 2390 2400 QKPLEAELHN CVVVQFDGPM SFYVQMESDV PALEQMTDKL LDAEQDLPAF SDLKEGALCV 2410 2420 2430 2440 2450 2460 AQFPEDEVFY RAQIRKVLDD GKCEVHFIDF GNNAVTQQFR QLPEELAKPA RYSRHCELDA 2470 2480 2490 2500 2510 STISKCDAAL LQSFIDTRFS ETFQVEILAT KGTGTHVVRL FYQSKNISEK LQECQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tudor, a posterior-group gene of Drosophila melanogaster, encodes a novel protein and an mRNA localized during mid-oogenesis." Golumbeski G.S., Bardsley A., Tax F., Boswell R.E. Genes Dev. 5:2060-2070(1991) [PubMed: 1936993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"Valois, a component of the nuage and pole plasm, is involved in assembly of these structures, and binds to Tudor and the methyltransferase Capsuleen." Anne J., Mechler B.M. Development 132:2167-2177(2005) [PubMed: 15800004] [Abstract] Cited for: INTERACTION WITH VLS.
[5]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-226; SER-235; SER-239 AND SER-800, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X62420 mRNA. Translation: CAA44286.1.
AE013599 Genomic DNA. Translation: AAF46693.1.

PIR

A41519.

RefSeq

NP_476773.1. NM_057425.2.

UniGene

Dm.3354.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3NTH	X-ray	2.80	A	2344-2515	[»]
3NTI	X-ray	2.80	A	2344-2515	[»]
3NTK	X-ray	1.80	A/B	2346-2514	[»]

ProteinModelPortal

P25823.

SMR

P25823. Positions 412-553, 598-763, 1065-1114, 1309-1454, 1616-1783, 1796-1960, 1981-2135, 2165-2333, 2346-2514.

ModBase

Search...

Protein-protein interaction databases

IntAct

P25823. 1 interaction.

MINT

MINT-1334222.

STRING

P25823.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblMetazoa

FBtr0071582; FBpp0071508; FBgn0003891.

GeneID

37417.

KEGG

dme:Dmel_CG9450.

NMPDR

fig|7227.3.peg.6607.

UCSC

CG9450-RA. d. melanogaster.

Organism-specific databases

CTD

37417.

FlyBase

FBgn0003891. tud.

Phylogenomic databases

eggNOG

inNOG08159.

GeneTree

EMGT00050000002111.

InParanoid

P25823.

OMA

HFIDFGN.

OrthoDB

EOG4J9KDN.

PhylomeDB

P25823.

Gene expression databases

Bgee

P25823.

GermOnline

CG9450. Drosophila melanogaster.

Family and domain databases

InterPro

IPR008191. Maternal_tudor.
IPR002999. Tudor.
IPR018351. Tudor_subgr.
[Graphical view]

Pfam

PF00567. TUDOR. 9 hits.
[Graphical view]

SMART

SM00333. TUDOR. 10 hits.
[Graphical view]

PROSITE

PS50304. TUDOR. 9 hits.
[Graphical view]

ProtoNet

Search...

Other

NextBio

803531.

Entry information

Entry name

TUD_DROME

Accession

Primary (citable) accession number: P25823
Secondary accession number(s): Q9W2J8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	August 30, 2005
Last modified:	January 25, 2012
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project