P25822 (PUM_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Maternal protein pumilio

Gene names

Name:	pum
ORF Names:	CG9755

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	1533 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding to the Nanos Response Element (NRE), a 16 bp sequence in the hb mRNA 3'-UTR and prevents its translation. Required for abdominal development and to support proliferation and self-renewal of germ cells. Pum is the only gene required for nos activity that is not also required for posterior localization of germline determinants. Pum is required during embryogenesis when nos activity apparently moves anteriorly from the posterior pole. Ref.1 Ref.2 Ref.8
Subunit structure	Interacts with nos and brat. Acts via the formation of a quaternary complex composed of pum, nos, brat and the 3'-UTR mRNA of hb. Ref.9 Ref.10
Subcellular location	Cytoplasm. Note: It is concentrated in the cortical region of the embryo beneath the nuclei. Ref.1
Developmental stage	Expressed both maternally and zygotically in embryos. Ref.1 Ref.2
Domain	The pumilio repeats mediate the association with RNA by packing together to form a right-handed superhelix that approximates a half doughnut By similarity. Ref.8
Disruption phenotype	Flies display defective abdomen pattern formation and are embryonic lethal. Ref.2
Sequence similarities	Contains 1 PUM-HD domain. Contains 8 pumilio repeats.
Sequence caution	The sequence AAX33465.1 differs from that shown. Reason: Frameshift at position 1293.

Ontologies

Keywords
Biological process	Translation regulation
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	RNA-binding
Molecular function	Developmental protein Repressor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	anterior/posterior axis specification, embryo Traceable author statement PubMed 11858839. Source: FlyBase behavioral response to ethanol Inferred from mutant phenotype PubMed 18435628. Source: FlyBase cell fate determination Traceable author statement PubMed 11131529. Source: FlyBase dendrite morphogenesis Inferred from mutant phenotype PubMed 14972682. Source: FlyBase germ-line stem cell division Non-traceable author statement PubMed 11131516. Source: FlyBase head involution Inferred from mutant phenotype PubMed 12015297. Source: FlyBase long-term memory Inferred from mutant phenotype PubMed 12593794PubMed 21490205. Source: FlyBase mitosis Traceable author statement PubMed 11858839. Source: FlyBase negative regulation of cell cycle Traceable author statement PubMed 11858839. Source: FlyBase negative regulation of epidermal growth factor receptor signaling pathway Inferred from mutant phenotype PubMed 22514614. Source: FlyBase negative regulation of transcription, DNA-dependent Traceable author statement PubMed 12655640. Source: FlyBase nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Traceable author statement PubMed 11858839. Source: FlyBase oogenesis Traceable author statement PubMed 11131529PubMed 9988212. Source: FlyBase pole cell migration Inferred from mutant phenotype PubMed 10559987. Source: FlyBase positive regulation of translation Traceable author statement PubMed 11700288. Source: FlyBase regulation of synaptic growth at neuromuscular junction Inferred from direct assay PubMed 15541314. Source: FlyBase regulation of synaptic transmission Inferred from mutant phenotype PubMed 15470135. Source: FlyBase
Cellular_component	cytoplasm Inferred from direct assay Ref.1. Source: FlyBase
Molecular_function	mRNA 3'-UTR binding Traceable author statement PubMed 11131529PubMed 12655640. Source: FlyBase translation repressor activity, nucleic acid binding Traceable author statement PubMed 12414187. Source: FlyBase
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: P25822-1)

Also known as: C; D;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: P25822-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-348: Missing.
     349-372: AAMMPPQNQYMNSSAVAAANRNAA → MMKLLHDFILDARTAEDVALTQEM

Note: No experimental confirmation available.

Isoform E (identifier: P25822-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-598: Missing.
     599-659: GAYAAHQQMA...NLLQQQNFDV → MVVLETASAL...DVLSLAFNHN

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1533

1533

Maternal protein pumilio

PRO_0000075916

Regions

Domain

1091 – 1428

338

PUM-HD

Repeat

1111 – 1146

36

Pumilio 1

Repeat

1147 – 1182

36

Pumilio 2

Repeat

1183 – 1218

36

Pumilio 3

Repeat

1219 – 1254

36

Pumilio 4

Repeat

1255 – 1290

36

Pumilio 5

Repeat

1291 – 1326

36

Pumilio 6

Repeat

1327 – 1362

36

Pumilio 7

Repeat

1366 – 1402

37

Pumilio 8

Compositional bias

34 – 45

12

Ala-rich

Compositional bias

57 – 77

21

Gly/Val-rich

Compositional bias

83 – 93

11

Ala-rich

Compositional bias

130 – 174

45

Gly-rich

Compositional bias

152 – 164

13

Poly-Gly

Compositional bias

181 – 212

32

Ala-rich

Compositional bias

213 – 236

24

Gln-rich

Compositional bias

262 – 274

13

Poly-Gln

Compositional bias

571 – 599

29

Gly-rich

Compositional bias

708 – 725

18

Poly-Gln

Compositional bias

936 – 946

11

Poly-Ala

Compositional bias

1050 – 1062

13

Poly-Ala

Amino acid modifications

Modified residue

453

1

Phosphoserine Ref.11

Modified residue

468

1

Phosphoserine Ref.11

Modified residue

470

1

Phosphoserine Ref.11

Modified residue

477

1

Phosphoserine Ref.11

Modified residue

505

1

Phosphoserine Ref.11

Natural variations

Alternative sequence

1 – 598

598

Missing in isoform E.

VSP_009313

Alternative sequence

1 – 348

348

Missing in isoform B.

VSP_008216

Alternative sequence

349 – 372

24

AAMMP…NRNAA → MMKLLHDFILDARTAEDVAL TQEM in isoform B.

VSP_008217

Alternative sequence

599 – 659

61

GAYAA…QNFDV → MVVLETASALLGGPYAQGAP ALKMVQKRYIGLHHWLGPIR SKELKEHIVSDDVLSLAFNH N in isoform E.

VSP_009314

Experimental info

Mutagenesis

1127

1

R → A: Disrupts RNA-binding. Ref.12

Mutagenesis

1167

1

K → A: Disrupts RNA-binding. Ref.12

Mutagenesis

1199

1

R → A: Disrupts RNA-binding. Ref.12

Mutagenesis

1235

1

H → A: Disrupts RNA-binding. Ref.12

Mutagenesis

1330

1

G → D in Pum680; abolishes interaction with brat and translational repression activity but not RNA-binding activity. Ref.8 Ref.12

Mutagenesis

1346

1

E → K: Disrupts RNA-binding. Ref.12

Mutagenesis

1365

1

C → R: Abolishes interaction with brat. Ref.12

Mutagenesis

1366

1

T → D: Abolishes interaction with brat. Ref.12

Mutagenesis

1367

1

F → S: Abolishes interaction with nos. Ref.12

Mutagenesis

1368

1

N → S: Abolishes interaction with brat. Ref.12

Sequence conflict

362

1

S → A in CAA44474. Ref.1

Sequence conflict

1103

1

R → P in AAB59189. Ref.2

Sequence conflict

1165

1

I → S in AAX33465. Ref.6

Sequence conflict

1374

1

V → M in AAQ22439. Ref.5

Sequence conflict

1406 – 1407

2

PH → KN in CAA44474. Ref.1

Sequence conflict

1491

1

N → S in AAQ22439. Ref.5

Sequence conflict

1496

1

V → I in AAB59189. Ref.2

Sequence conflict

1519

1

S → G in AAB59189. Ref.2

Secondary structure

1

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1533

Helix Strand Turn

Details...

Helix

1094 – 1100

7

Helix

1109 – 1112

4

Helix

1116 – 1120

5

Helix

1123 – 1133

11

Helix

1138 – 1150

13

Helix

1152 – 1156

5

Helix

1161 – 1171

11

Helix

1174 – 1186

13

Helix

1189 – 1192

4

Helix

1195 – 1205

11

Helix

1210 – 1217

8

Helix

1218 – 1220

3

Helix

1224 – 1228

5

Helix

1233 – 1242

10

Helix

1246 – 1249

4

Helix

1250 – 1256

7

Turn

1257 – 1259

3

Helix

1260 – 1264

5

Helix

1269 – 1279

11

Turn

1282 – 1284

3

Helix

1286 – 1294

9

Turn

1295 – 1301

7

Turn

1303 – 1305

3

Helix

1306 – 1315

10

Helix

1318 – 1328

11

Helix

1332 – 1336

5

Helix

1341 – 1351

11

Helix

1354 – 1364

11

Turn

1369 – 1372

4

Helix

1373 – 1377

5

Helix

1381 – 1390

10

Helix

1394 – 1399

6

Sequences

	Sequence		Length	Mass (Da)
	Isoform A (C) (D) [UniParc]. Last modified September 19, 2003. Version 2. Checksum: 0A343220BB1F0B27	FASTA	1,533	157,528
	10 20 30 40 50 60 MKFLGGNDDR NGRGGVGVGT DAIVGSRGGV SQDAADAAGA AAAAAVGYVF QQRPSPGGVG 70 80 90 100 110 120 VGVGGVGGGV PGVGAVGSTL HEAAAAEYAA HFAQKQQQTR WACGDDGHGI DNPDKWKYNP 130 140 150 160 170 180 PMNPANAAPG GPPGNGSNGG PGAIGTIGMG SGLGGGGGGG AGGGNNGGSG TNGGLHHQSM 190 200 210 220 230 240 AAAAANMAAM QQAAALAKHN HMISQAAAAV AAQQQHQHPH QQHPQQQQQQ QQAQNQGHPH 250 260 270 280 290 300 HLMGGGNGLG NGNGLGIQHP GQQQQQQQQQ QQQQHPGQYN ANLLNHAAAL GHMSSYAQSG 310 320 330 340 350 360 GSMYDHHGGA MHPGMNGGMP KQQPLGPPGA GGPQDYVYMG GQTTVPMGAA MMPPQNQYMN 370 380 390 400 410 420 SSAVAAANRN AAITTSTAKK LWEKSDGKGV SSSTPGGPLH PLQIPGIGDP SSVWKDHTWS 430 440 450 460 470 480 TQGENILVPP PSRAYAHGGA SDTSNSGNAG ILSPRDSTCA KVVEYVFSGS PTNKDSSLSG 490 500 510 520 530 540 LEPHLRNLKF DDNDKSRDDK EKANSPFDTN GLKKDDQVTN SNGVVNGIDD DKGFNRTPGS 550 560 570 580 590 600 RQPSPAEESQ PRPPNLLFPP LPFNHMLMDH GQGMGGGLGG VVGSGNGVGG GSGGGGAGGA 610 620 630 640 650 660 YAAHQQMAAQ MSQLQPPMMN GVGGGMPMAA QSPMLNHQAA GPNHMESPGN LLQQQNFDVQ 670 680 690 700 710 720 QLFRSQNPGL AAVATNAAAA AAAAAAATSA ASAAAAVGAP PVPNGSLQQS QQQQQQQQQQ 730 740 750 760 770 780 QQQQQMHMAA ASQQFLAAQQ QAQNAAYAAQ QATSYVINPG QEAAPYMGMI AAAQMPYYGV 790 800 810 820 830 840 APWGMYPGNL IPQQGTQPRR PLTPSQQGAE NQPYQVIPAF LDHTGSLLMG GPRTGTPMRL 850 860 870 880 890 900 VSPAPVLVPP GATRAGPPPP QGPQLYQPQP QTAQQNLYSQ QNGSSVGGLA LNTSSLTGRR 910 920 930 940 950 960 DSFDRSTSAF SPSTMDYTSS GVAAAANAVN STVAQAAAAA AAAAAARGKW PGAMSGAASG 970 980 990 1000 1010 1020 AYGALGAGNA SASPLGAPIT PPPSAQSCLL GSRAPGAESR QRQQQQQQLA AVGLPATAAA 1030 1040 1050 1060 1070 1080 AQAAVAAAAN NMFGSNSSIF SNPLAIPGTA AVAAAAAAAA AANSRQVAAT AAAAAAVAAA 1090 1100 1110 1120 1130 1140 AGGVGGAPQP GRSRLLEDFR NQRYPNLQLR DLANHIVEFS QDQHGSRFIQ QKLERATAAE 1150 1160 1170 1180 1190 1200 KQMVFSEILA AAYSLMTDVF GNYVIQKFFE FGTPEQKNTL GMQVKGHVLQ LALQMYGCRV 1210 1220 1230 1240 1250 1260 IQKALESISP EQQQEIVHEL DGHVLKCVKD QNGNHVVQKC IECVDPVALQ FIINAFKGQV 1270 1280 1290 1300 1310 1320 YSLSTHPYGC RVIQRILEHC TAEQTTPILD ELHEHTEQLI QDQYGNYVIQ HVLEHGKQED 1330 1340 1350 1360 1370 1380 KSILINSVRG KVLVLSQHKF ASNVVEKCVT HATRGERTGL IDEVCTFNDN ALHVMMKDQY 1390 1400 1410 1420 1430 1440 ANYVVQKMID VSEPTQLKKL MTKIRPHMAA LRKYTYGKHI NAKLEKYYMK ITNPITVGTG 1450 1460 1470 1480 1490 1500 AGGVPAASSA AAVSSGATSA SVTACTSGSS TTTTSTTNSL ASPTICSVQE NGSAMVVEPS 1510 1520 1530 SPDASESSSS VVSGAVNSSL GPIGPPTNGN VVL
	Isoform B [UniParc]. Checksum: 850A3AEDEC837AD0	FASTA	1,185	123,520
	10 20 30 40 50 60 MMKLLHDFIL DARTAEDVAL TQEMITTSTA KKLWEKSDGK GVSSSTPGGP LHPLQIPGIG 70 80 90 100 110 120 DPSSVWKDHT WSTQGENILV PPPSRAYAHG GASDTSNSGN AGILSPRDST CAKVVEYVFS 130 140 150 160 170 180 GSPTNKDSSL SGLEPHLRNL KFDDNDKSRD DKEKANSPFD TNGLKKDDQV TNSNGVVNGI 190 200 210 220 230 240 DDDKGFNRTP GSRQPSPAEE SQPRPPNLLF PPLPFNHMLM DHGQGMGGGL GGVVGSGNGV 250 260 270 280 290 300 GGGSGGGGAG GAYAAHQQMA AQMSQLQPPM MNGVGGGMPM AAQSPMLNHQ AAGPNHMESP 310 320 330 340 350 360 GNLLQQQNFD VQQLFRSQNP GLAAVATNAA AAAAAAAAAT SAASAAAAVG APPVPNGSLQ 370 380 390 400 410 420 QSQQQQQQQQ QQQQQQQMHM AAASQQFLAA QQQAQNAAYA AQQATSYVIN PGQEAAPYMG 430 440 450 460 470 480 MIAAAQMPYY GVAPWGMYPG NLIPQQGTQP RRPLTPSQQG AENQPYQVIP AFLDHTGSLL 490 500 510 520 530 540 MGGPRTGTPM RLVSPAPVLV PPGATRAGPP PPQGPQLYQP QPQTAQQNLY SQQNGSSVGG 550 560 570 580 590 600 LALNTSSLTG RRDSFDRSTS AFSPSTMDYT SSGVAAAANA VNSTVAQAAA AAAAAAAARG 610 620 630 640 650 660 KWPGAMSGAA SGAYGALGAG NASASPLGAP ITPPPSAQSC LLGSRAPGAE SRQRQQQQQQ 670 680 690 700 710 720 LAAVGLPATA AAAQAAVAAA ANNMFGSNSS IFSNPLAIPG TAAVAAAAAA AAAANSRQVA 730 740 750 760 770 780 ATAAAAAAVA AAAGGVGGAP QPGRSRLLED FRNQRYPNLQ LRDLANHIVE FSQDQHGSRF 790 800 810 820 830 840 IQQKLERATA AEKQMVFSEI LAAAYSLMTD VFGNYVIQKF FEFGTPEQKN TLGMQVKGHV 850 860 870 880 890 900 LQLALQMYGC RVIQKALESI SPEQQQEIVH ELDGHVLKCV KDQNGNHVVQ KCIECVDPVA 910 920 930 940 950 960 LQFIINAFKG QVYSLSTHPY GCRVIQRILE HCTAEQTTPI LDELHEHTEQ LIQDQYGNYV 970 980 990 1000 1010 1020 IQHVLEHGKQ EDKSILINSV RGKVLVLSQH KFASNVVEKC VTHATRGERT GLIDEVCTFN 1030 1040 1050 1060 1070 1080 DNALHVMMKD QYANYVVQKM IDVSEPTQLK KLMTKIRPHM AALRKYTYGK HINAKLEKYY 1090 1100 1110 1120 1130 1140 MKITNPITVG TGAGGVPAAS SAAAVSSGAT SASVTACTSG SSTTTTSTTN SLASPTICSV 1150 1160 1170 1180 QENGSAMVVE PSSPDASESS SSVVSGAVNS SLGPIGPPTN GNVVL
	Isoform E [UniParc]. Checksum: 22ECEC73646072EF	FASTA	935	97,754
	10 20 30 40 50 60 MVVLETASAL LGGPYAQGAP ALKMVQKRYI GLHHWLGPIR SKELKEHIVS DDVLSLAFNH 70 80 90 100 110 120 NQQLFRSQNP GLAAVATNAA AAAAAAAAAT SAASAAAAVG APPVPNGSLQ QSQQQQQQQQ 130 140 150 160 170 180 QQQQQQQMHM AAASQQFLAA QQQAQNAAYA AQQATSYVIN PGQEAAPYMG MIAAAQMPYY 190 200 210 220 230 240 GVAPWGMYPG NLIPQQGTQP RRPLTPSQQG AENQPYQVIP AFLDHTGSLL MGGPRTGTPM 250 260 270 280 290 300 RLVSPAPVLV PPGATRAGPP PPQGPQLYQP QPQTAQQNLY SQQNGSSVGG LALNTSSLTG 310 320 330 340 350 360 RRDSFDRSTS AFSPSTMDYT SSGVAAAANA VNSTVAQAAA AAAAAAAARG KWPGAMSGAA 370 380 390 400 410 420 SGAYGALGAG NASASPLGAP ITPPPSAQSC LLGSRAPGAE SRQRQQQQQQ LAAVGLPATA 430 440 450 460 470 480 AAAQAAVAAA ANNMFGSNSS IFSNPLAIPG TAAVAAAAAA AAAANSRQVA ATAAAAAAVA 490 500 510 520 530 540 AAAGGVGGAP QPGRSRLLED FRNQRYPNLQ LRDLANHIVE FSQDQHGSRF IQQKLERATA 550 560 570 580 590 600 AEKQMVFSEI LAAAYSLMTD VFGNYVIQKF FEFGTPEQKN TLGMQVKGHV LQLALQMYGC 610 620 630 640 650 660 RVIQKALESI SPEQQQEIVH ELDGHVLKCV KDQNGNHVVQ KCIECVDPVA LQFIINAFKG 670 680 690 700 710 720 QVYSLSTHPY GCRVIQRILE HCTAEQTTPI LDELHEHTEQ LIQDQYGNYV IQHVLEHGKQ 730 740 750 760 770 780 EDKSILINSV RGKVLVLSQH KFASNVVEKC VTHATRGERT GLIDEVCTFN DNALHVMMKD 790 800 810 820 830 840 QYANYVVQKM IDVSEPTQLK KLMTKIRPHM AALRKYTYGK HINAKLEKYY MKITNPITVG 850 860 870 880 890 900 TGAGGVPAAS SAAAVSSGAT SASVTACTSG SSTTTTSTTN SLASPTICSV QENGSAMVVE 910 920 930 PSSPDASESS SSVVSGAVNS SLGPIGPPTN GNVVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila pumilio gene: an unusually long transcription unit and an unusual protein." Macdonald P.M. Development 114:221-232(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[2]	"Pumilio is essential for function but not for distribution of the Drosophila abdominal determinant Nanos." Barker D.D., Wang C., Moore J., Dickinson L.K., Lehmann R. Genes Dev. 6:2312-2326(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE. Tissue: Embryo.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND E). Strain: Berkeley. Tissue: Embryo.
[6]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Embryo.
[7]	"The Pumilio protein binds RNA through a conserved domain that defines a new class of RNA-binding proteins." Zamore P.D., Williamson J.R., Lehmann R. RNA 3:1421-1433(1997) [PubMed] [Europe PMC] [Abstract] Cited for: RNA-BINDING.
[8]	"The Pumilio RNA-binding domain is also a translational regulator." Wharton R.P., Sonoda J., Lee T., Patterson M., Murata Y. Mol. Cell 1:863-872(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DOMAIN, MUTAGENESIS OF GLY-1330.
[9]	"Recruitment of Nanos to hunchback mRNA by Pumilio." Sonoda J., Wharton R.P. Genes Dev. 13:2704-2712(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NOS, RNA-BINDING.
[10]	"Drosophila Brain tumor is a translational repressor." Sonoda J., Wharton R.P. Genes Dev. 15:762-773(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH BRAT AND NOS.
[11]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-453; SER-468; SER-470; SER-477 AND SER-505, MASS SPECTROMETRY. Tissue: Embryo.
[12]	"Structure of Pumilio reveals similarity between RNA and peptide binding motifs." Edwards T.A., Pyle S.E., Wharton R.P., Aggarwal A.K. Cell 105:281-289(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 1092-1411, MUTAGENESIS OF ARG-1127; LYS-1167; ARG-1199; HIS-1235; GLY-1330; GLU-1346; CYS-1365; THR-1366; PHE-1367 AND ASN-1368.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X62589 mRNA. Translation: CAA44474.1.
L07943 mRNA. Translation: AAB59189.1.
AE014297 Genomic DNA. Translation: AAF54340.2.
AE014297 Genomic DNA. Translation: AAF54338.2.
AE014297 Genomic DNA. Translation: AAN13409.1.
AE014297 Genomic DNA. Translation: AAN13410.1.
AE014297 Genomic DNA. Translation: AAO41523.1.
BT001872 mRNA. Translation: AAN71644.1.
BT009970 mRNA. Translation: AAQ22439.1.
BT021317 mRNA. Translation: AAX33465.1. Frameshift.

PIR

A46221.

RefSeq

NP_001262403.1. NM_001275474.1.
NP_524285.2. NM_079561.4.
NP_731314.1. NM_169258.3.
NP_731315.1. NM_169259.3.
NP_731316.2. NM_169260.4.
NP_788604.1. NM_176427.3.

UniGene

Dm.1115.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3H3D	X-ray	2.30	X/Y	1093-1411	[»]

ProteinModelPortal

P25822.

SMR

P25822. Positions 1093-1404.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-822094.

Proteomic databases

PaxDb

P25822.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblMetazoa

FBtr0081990; FBpp0081470; FBgn0003165.
FBtr0081991; FBpp0081471; FBgn0003165.
FBtr0081992; FBpp0081472; FBgn0003165.
FBtr0333667; FBpp0305823; FBgn0003165.

GeneID

41094.

KEGG

dme:Dmel_CG9755.

UCSC

CG9755-RA. d. melanogaster.

Organism-specific databases

CTD

41094.

FlyBase

FBgn0003165. pum.

Phylogenomic databases

eggNOG

COG5099.

GeneTree

ENSGT00390000017241.

InParanoid

P25822.

OMA

LASPTIC.

OrthoDB

EOG4HMGRT.

PhylomeDB

P25822.

Gene expression databases

Bgee

P25822.

GermOnline

CG9755. Drosophila melanogaster.

Family and domain databases

Gene3D

1.25.10.10. 1 hit.

InterPro

IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR001313. Pumilio_RNA-bd_rpt.
[Graphical view]

Pfam

PF00806. PUF. 8 hits.
[Graphical view]

SMART

SM00025. Pumilio. 8 hits.
[Graphical view]

SUPFAM

SSF48371. ARM-type_fold. 1 hit.

PROSITE

PS50302. PUM. 8 hits.
PS50303. PUM_HD. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P25822.

GenomeRNAi

41094.

NextBio

822132.

Entry information

Entry name

PUM_DROME

Accession

Primary (citable) accession number: P25822
Secondary accession number(s): A4V2K5 , Q5BIA7, Q7YU65, Q8IGA8, Q9VHH4, Q9VHH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	September 19, 2003
Last modified:	May 1, 2013
This is version 124 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project