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Protein

Glutamine synthetase

Gene

glnA

Organism
Neisseria gonorrhoeae
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Enzyme regulationi

The activity of this enzyme could be controlled by adenylation under conditions of abundant glutamine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi133Magnesium 1By similarity1
Metal bindingi135Magnesium 2By similarity1
Binding sitei211ATPBy similarity1
Metal bindingi216Magnesium 2By similarity1
Metal bindingi224Magnesium 2By similarity1
Binding sitei269L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi273Magnesium 1; via pros nitrogenBy similarity1
Binding sitei277ATPBy similarity1
Binding sitei325L-glutamateBy similarity1
Binding sitei331L-glutamateBy similarity1
Binding sitei343ATPBy similarity1
Binding sitei343L-glutamateBy similarity1
Binding sitei348ATPBy similarity1
Binding sitei356ATPBy similarity1
Metal bindingi361Magnesium 1By similarity1
Binding sitei363L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi275 – 277ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseCurated
Glutamine synthetase I betaBy similarity
Short name:
GSI betaBy similarity
Gene namesi
Name:glnABy similarity
OrganismiNeisseria gonorrhoeae
Taxonomic identifieri485 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532481 – 472Glutamine synthetaseAdd BLAST472

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei401O-AMP-tyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexameric ring.By similarity

Structurei

3D structure databases

ProteinModelPortaliP25821
SMRiP25821
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni268 – 269L-glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiENOG4105C5F Bacteria
COG0174 LUCA

Family and domain databases

Gene3Di3.10.20.70, 1 hit
InterProiView protein in InterPro
IPR008147 Gln_synt_b-grasp
IPR036651 Gln_synt_N
IPR014746 Gln_synth/guanido_kin_cat_dom
IPR008146 Gln_synth_cat_dom
IPR027303 Gln_synth_gly_rich_site
IPR004809 Gln_synth_I
IPR001637 Gln_synth_I_adenylation_site
IPR027302 Gln_synth_N_conserv_site
PfamiView protein in Pfam
PF00120 Gln-synt_C, 1 hit
PF03951 Gln-synt_N, 1 hit
SMARTiView protein in SMART
SM01230 Gln-synt_C, 1 hit
SUPFAMiSSF54368 SSF54368, 1 hit
SSF55931 SSF55931, 1 hit
TIGRFAMsiTIGR00653 GlnA, 1 hit
PROSITEiView protein in PROSITE
PS00180 GLNA_1, 1 hit
PS00182 GLNA_ADENYLATION, 1 hit
PS00181 GLNA_ATP, 1 hit

Sequencei

Sequence statusi: Complete.

P25821-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIKNAVKLI EKSKARFVDL RFTDTKGKQH HFTVPARIVL EGPEEWFENG
60 70 80 90 100
QAFDGSSIGG WKGIQASDMQ LRPDASTAFV DPFYDDVTVV ITCDVIDPAD
110 120 130 140 150
GQGYDRDPRS IARRAEAYLK SSGIGDTAYF GPEPEFFVFD GVEFETDMHK
160 170 180 190 200
TRYEITSESG AWASGLHMDG QNTGHRPAVK GGYAPVAPID CGQDLRSAMV
210 220 230 240 250
NILEGLGIEV EVHHSEVGTG SQMEIGTRFA TLVKRADQTQ DMKYVIQNVA
260 270 280 290 300
HNFGKTATFM PKPIMGDNGS GMHVHQSIWK DGQNLFAGDG YAGLSDTALY
310 320 330 340 350
YIGGIIKHAK ALNAITNPST NSYKRLVPHF EAPTKLAYSA KNRSASIRIP
360 370 380 390 400
SVNSSKARRI EARFPDPTAN PYLAFAALLM AGLDGIQNKI HPGDPADKNL
410 420 430 440 450
YDLPPEEDAL VPTVCASLEE ALAALKADHE FLLRGGVFSK DWIDSYIAFK
460 470
EEDVRRIRMA PHPLEFEMYY SL
Length:472
Mass (Da):51,975
Last modified:May 1, 1992 - v1
Checksum:iE1643ED5CF240108
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84113 Genomic DNA Translation: AAB61772.1
RefSeqiWP_003689535.1, NZ_NGQH01000029.1

Similar proteinsi

Entry informationi

Entry nameiGLN1B_NEIGO
AccessioniPrimary (citable) accession number: P25821
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: March 28, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health