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Protein

Aquaporin TIP1-1

Gene

TIP1-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Water channel required to facilitate the transport of water, diffusion of amino acids and/or peptides from the vacuolar compartment to the cytoplasm. Does not promote glycerol permeability. May play a role in the control of cell turgor and cell expansion. Its function is impaired by Hg2+. May be involved in a vesicle-based metabolite routing through or between pre-vacuolar compartments and the central vacuole. Transports urea in yeast cells in a pH-independent manner. Transports H2O2 in yeast cells.4 Publications

GO - Molecular functioni

  • glycerol channel activity Source: GO_Central
  • urea transmembrane transporter activity Source: TAIR
  • water channel activity Source: TAIR

GO - Biological processi

  • glycerol transport Source: GOC
  • hydrogen peroxide transmembrane transport Source: TAIR
  • ion transmembrane transport Source: GO_Central
  • urea transmembrane transport Source: GOC
  • urea transport Source: TAIR
  • water transport Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-ATH-1237044. Erythrocytes take up carbon dioxide and release oxygen.
R-ATH-1247673. Erythrocytes take up oxygen and release carbon dioxide.
R-ATH-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-ATH-432047. Passive transport by Aquaporins.

Protein family/group databases

TCDBi1.A.8.10.3. the major intrinsic protein (mip) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Aquaporin TIP1-1
Alternative name(s):
Aquaporin TIP
Gamma-tonoplast intrinsic protein
Short name:
Gamma-TIP
Tonoplast intrinsic protein 1-1
Short name:
AtTIP1;1
Tonoplast intrinsic protein, root-specific RB7
Cleaved into the following chain:
Gene namesi
Name:TIP1-1
Synonyms:AQP.1
Ordered Locus Names:At2g36830
ORF Names:T1J8.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G36830.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2323CytoplasmicSequence analysisAdd
BLAST
Transmembranei24 – 4421Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini45 – 5612VacuolarSequence analysisAdd
BLAST
Transmembranei57 – 7721Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini78 – 10326CytoplasmicSequence analysisAdd
BLAST
Transmembranei104 – 12421Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini125 – 14319VacuolarSequence analysisAdd
BLAST
Transmembranei144 – 16421Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini165 – 1728CytoplasmicSequence analysis
Transmembranei173 – 19321Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini194 – 21825VacuolarSequence analysisAdd
BLAST
Transmembranei219 – 23921Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini240 – 25112CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • central vacuole Source: TAIR
  • chloroplast envelope Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • plant-type vacuole membrane Source: TAIR
  • protein storage vacuole Source: TAIR
  • vacuolar membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

Pathology & Biotechi

Disruption phenotypei

Plants display lesion formation or plant death, and low contents of glucose, fructose, inositol, and threonic, succinic, fumaric, and malic acids.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi118 – 1181C → T: Strongly reduces the mercury-sensitivity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251Aquaporin TIP1-1PRO_0000064008Add
BLAST
Initiator methionineiRemoved; alternateBy similarity
Chaini2 – 251250Aquaporin TIP1-1, N-terminally processedPRO_0000425756Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP25818.
PRIDEiP25818.

Expressioni

Tissue specificityi

In all the vegetative organs, but not in seeds. Preferentially expressed in roots.1 Publication

Inductioni

By gibberellins.

Gene expression databases

GenevisibleiP25818. AT.

Interactioni

Subunit structurei

Interacts with cucumber mosaic virus (CMV) Protein 1a.1 Publication

Protein-protein interaction databases

BioGridi3599. 12 interactions.
IntActiP25818. 1 interaction.
STRINGi3702.AT2G36830.1.

Structurei

3D structure databases

ProteinModelPortaliP25818.
SMRiP25818. Positions 21-235.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi85 – 873NPA 1
Motifi199 – 2013NPA 2

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
HOGENOMiHOG000288286.
InParanoidiP25818.
KOiK09873.
OMAiACLILKF.
PhylomeDBiP25818.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P25818-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIRNIAIGR PDEATRPDAL KAALAEFIST LIFVVAGSGS GMAFNKLTEN
60 70 80 90 100
GATTPSGLVA AAVAHAFGLF VAVSVGANIS GGHVNPAVTF GAFIGGNITL
110 120 130 140 150
LRGILYWIAQ LLGSVVACLI LKFATGGLAV PAFGLSAGVG VLNAFVFEIV
160 170 180 190 200
MTFGLVYTVY ATAIDPKNGS LGTIAPIAIG FIVGANILAG GAFSGASMNP
210 220 230 240 250
AVAFGPAVVS WTWTNHWVYW AGPLVGGGIA GLIYEVFFIN TTHEQLPTTD

Y
Length:251
Mass (Da):25,620
Last modified:May 1, 1992 - v1
Checksum:iCECC6BAF42F23302
GO

Sequence cautioni

The sequence CAA38633.1 differs from that shown. Reason: Frameshift at position 244. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81I → V in CAA51171 (PubMed:8155880).Curated
Sequence conflicti63 – 631V → L in CAA51171 (PubMed:8155880).Curated
Sequence conflicti63 – 631V → L in AAM65100 (Ref. 7) Curated
Sequence conflicti96 – 961G → W in CAA81194 (PubMed:8580968).Curated
Sequence conflicti131 – 1311P → R in CAA81194 (PubMed:8580968).Curated
Sequence conflicti132 – 1321A → P in CAA38633 (PubMed:2129561).Curated
Sequence conflicti132 – 1321A → P in CAA81194 (PubMed:8580968).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54854 mRNA. Translation: CAA38633.1. Frameshift.
X63552 Genomic DNA. Translation: CAA45115.1.
M84344 Genomic DNA. Translation: AAA32806.1.
X72581 mRNA. Translation: CAA51171.1.
AC006922 Genomic DNA. Translation: AAD31569.1.
CP002685 Genomic DNA. Translation: AEC09303.1.
AF370172 mRNA. Translation: AAK43987.1.
AY059134 mRNA. Translation: AAL15240.1.
AY087558 mRNA. Translation: AAM65100.1.
Z18124 mRNA. Translation: CAA79107.1.
Z18771 mRNA. Translation: CAA79247.1.
Z26215 mRNA. Translation: CAA81194.1.
PIRiS13718.
S22202.
RefSeqiNP_181221.1. NM_129238.3.
UniGeneiAt.25221.
At.43252.
At.67051.

Genome annotation databases

EnsemblPlantsiAT2G36830.1; AT2G36830.1; AT2G36830.
GeneIDi818255.
GrameneiAT2G36830.1; AT2G36830.1; AT2G36830.
KEGGiath:AT2G36830.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54854 mRNA. Translation: CAA38633.1. Frameshift.
X63552 Genomic DNA. Translation: CAA45115.1.
M84344 Genomic DNA. Translation: AAA32806.1.
X72581 mRNA. Translation: CAA51171.1.
AC006922 Genomic DNA. Translation: AAD31569.1.
CP002685 Genomic DNA. Translation: AEC09303.1.
AF370172 mRNA. Translation: AAK43987.1.
AY059134 mRNA. Translation: AAL15240.1.
AY087558 mRNA. Translation: AAM65100.1.
Z18124 mRNA. Translation: CAA79107.1.
Z18771 mRNA. Translation: CAA79247.1.
Z26215 mRNA. Translation: CAA81194.1.
PIRiS13718.
S22202.
RefSeqiNP_181221.1. NM_129238.3.
UniGeneiAt.25221.
At.43252.
At.67051.

3D structure databases

ProteinModelPortaliP25818.
SMRiP25818. Positions 21-235.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3599. 12 interactions.
IntActiP25818. 1 interaction.
STRINGi3702.AT2G36830.1.

Protein family/group databases

TCDBi1.A.8.10.3. the major intrinsic protein (mip) family.

Proteomic databases

PaxDbiP25818.
PRIDEiP25818.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G36830.1; AT2G36830.1; AT2G36830.
GeneIDi818255.
GrameneiAT2G36830.1; AT2G36830.1; AT2G36830.
KEGGiath:AT2G36830.

Organism-specific databases

TAIRiAT2G36830.

Phylogenomic databases

eggNOGiKOG0223. Eukaryota.
COG0580. LUCA.
HOGENOMiHOG000288286.
InParanoidiP25818.
KOiK09873.
OMAiACLILKF.
PhylomeDBiP25818.

Enzyme and pathway databases

ReactomeiR-ATH-1237044. Erythrocytes take up carbon dioxide and release oxygen.
R-ATH-1247673. Erythrocytes take up oxygen and release carbon dioxide.
R-ATH-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-ATH-432047. Passive transport by Aquaporins.

Miscellaneous databases

PROiP25818.

Gene expression databases

GenevisibleiP25818. AT.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Root-specific genes from tobacco and Arabidopsis homologous to an evolutionarily conserved gene family of membrane channel proteins."
    Yamamoto Y.T., Cheng C.-L., Conkling M.A.
    Nucleic Acids Res. 18:7449-7449(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
    Tissue: Root.
  2. "Vegetative and seed-specific forms of tonoplast intrinsic protein in the vacuolar membrane of Arabidopsis thaliana."
    Hoefte H.R., Hubbard L., Reizer J., Ludevid D., Kerman E.M., Chrispeels M.J.
    Plant Physiol. 99:561-570(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  3. "Cloning of two gibberellin-regulated cDNAs from Arabidopsis thaliana by subtractive hybridization: expression of the tonoplast water channel, gamma-TIP, is increased by GA3."
    Phillips A.L., Huttly A.K.
    Plant Mol. Biol. 24:603-615(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Landsberg erecta.
    Tissue: Flower bud.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-116 AND 243-251.
    Strain: cv. Columbia.
    Tissue: Seedling.
  9. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 96-207.
    Strain: cv. Columbia.
    Tissue: Seedling.
  10. "The vacuolar membrane protein gamma-TIP creates water specific channels in Xenopus oocytes."
    Maurel C., Reizer J., Schroeder J.I., Chrispeels M.J.
    EMBO J. 12:2241-2247(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Characterization of a new vacuolar membrane aquaporin sensitive to mercury at a unique site."
    Daniels M.J., Chaumont F., Mirkov T.E., Chrispeels M.J.
    Plant Cell 8:587-599(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, MUTAGENESIS OF CYS-118.
  12. "From genome to function: the Arabidopsis aquaporins."
    Quigley F., Rosenberg J.M., Shachar-Hill Y., Bohnert H.J.
    Genome Biol. 3:RESEARCH0001.1-RESEARCH0001.17(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.
  13. "Urea transport by nitrogen-regulated tonoplast intrinsic proteins in Arabidopsis."
    Liu L.-H., Ludewig U., Gassert B., Frommer W.B., von Wiren N.
    Plant Physiol. 133:1220-1228(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. "Loss of TIP1;1 aquaporin in Arabidopsis leads to cell and plant death."
    Ma S., Quist T.M., Ulanov A., Joly R., Bohnert H.J.
    Plant J. 40:845-859(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION.
  15. "Arabidopsis tonoplast proteins TIP1 and TIP2 interact with the cucumber mosaic virus 1a replication protein."
    Kim M.J., Kim H.R., Paek K.-H.
    J. Gen. Virol. 87:3425-3431(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CMV PROTEIN 1A.
  16. "Specific aquaporins facilitate the diffusion of hydrogen peroxide across membranes."
    Bienert G.P., Moeller A.L.B., Kristiansen K.A., Schulz A., Moeller I.M., Schjoerring J.K., Jahn T.P.
    J. Biol. Chem. 282:1183-1192(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiTIP11_ARATH
AccessioniPrimary (citable) accession number: P25818
Secondary accession number(s): P21652, Q42075, Q8L5T8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: February 17, 2016
This is version 156 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.