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Protein

Aquaporin TIP1-1

Gene

TIP1-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Water channel required to facilitate the transport of water, diffusion of amino acids and/or peptides from the vacuolar compartment to the cytoplasm. Does not promote glycerol permeability. May play a role in the control of cell turgor and cell expansion. Its function is impaired by Hg2+. May be involved in a vesicle-based metabolite routing through or between pre-vacuolar compartments and the central vacuole. Transports urea in yeast cells in a pH-independent manner. Transports H2O2 in yeast cells.4 Publications

GO - Molecular functioni

  • urea transmembrane transporter activity Source: TAIR
  • water channel activity Source: TAIR

GO - Biological processi

  • hydrogen peroxide transmembrane transport Source: TAIR
  • ion transmembrane transport Source: GO_Central
  • urea transport Source: TAIR
  • water transport Source: TAIR

Keywordsi

Biological processTransport

Enzyme and pathway databases

ReactomeiR-ATH-1237044 Erythrocytes take up carbon dioxide and release oxygen
R-ATH-1247673 Erythrocytes take up oxygen and release carbon dioxide
R-ATH-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-ATH-432047 Passive transport by Aquaporins

Protein family/group databases

TCDBi1.A.8.10.3 the major intrinsic protein (mip) family

Names & Taxonomyi

Protein namesi
Recommended name:
Aquaporin TIP1-1
Alternative name(s):
Aquaporin TIP
Gamma-tonoplast intrinsic protein
Short name:
Gamma-TIP
Tonoplast intrinsic protein 1-1
Short name:
AtTIP1;1
Tonoplast intrinsic protein, root-specific RB7
Gene namesi
Name:TIP1-1
Synonyms:AQP.1
Ordered Locus Names:At2g36830
ORF Names:T1J8.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G36830
TAIRilocus:2057906 AT2G36830

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 23CytoplasmicSequence analysisAdd BLAST23
Transmembranei24 – 44Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini45 – 56VacuolarSequence analysisAdd BLAST12
Transmembranei57 – 77Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini78 – 103CytoplasmicSequence analysisAdd BLAST26
Transmembranei104 – 124Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini125 – 143VacuolarSequence analysisAdd BLAST19
Transmembranei144 – 164Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini165 – 172CytoplasmicSequence analysis8
Transmembranei173 – 193Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini194 – 218VacuolarSequence analysisAdd BLAST25
Transmembranei219 – 239Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini240 – 251CytoplasmicSequence analysisAdd BLAST12

Keywords - Cellular componenti

Membrane, Vacuole

Pathology & Biotechi

Disruption phenotypei

Plants display lesion formation or plant death, and low contents of glucose, fructose, inositol, and threonic, succinic, fumaric, and malic acids.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi118C → T: Strongly reduces the mercury-sensitivity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000640081 – 251Aquaporin TIP1-1Add BLAST251

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP25818
PRIDEiP25818

Expressioni

Tissue specificityi

In all the vegetative organs, but not in seeds. Preferentially expressed in roots.1 Publication

Inductioni

By gibberellins.

Gene expression databases

ExpressionAtlasiP25818 baseline and differential
GenevisibleiP25818 AT

Interactioni

Subunit structurei

Interacts with cucumber mosaic virus (CMV) Protein 1a.1 Publication

Protein-protein interaction databases

BioGridi3599, 12 interactors
IntActiP25818, 3 interactors
STRINGi3702.AT2G36830.1

Structurei

3D structure databases

ProteinModelPortaliP25818
SMRiP25818
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi85 – 87NPA 13
Motifi199 – 201NPA 23

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0223 Eukaryota
COG0580 LUCA
HOGENOMiHOG000288286
InParanoidiP25818
KOiK09873
OMAiINQTHEP
OrthoDBiEOG09360J95
PhylomeDBiP25818

Family and domain databases

CDDicd00333 MIP, 1 hit
Gene3Di1.20.1080.10, 1 hit
InterProiView protein in InterPro
IPR023271 Aquaporin-like
IPR034294 Aquaporin_transptr
IPR000425 MIP
IPR022357 MIP_CS
PANTHERiPTHR19139 PTHR19139, 1 hit
PfamiView protein in Pfam
PF00230 MIP, 1 hit
PRINTSiPR00783 MINTRINSICP
SUPFAMiSSF81338 SSF81338, 1 hit
PROSITEiView protein in PROSITE
PS00221 MIP, 1 hit

Sequencei

Sequence statusi: Complete.

P25818-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIRNIAIGR PDEATRPDAL KAALAEFIST LIFVVAGSGS GMAFNKLTEN
60 70 80 90 100
GATTPSGLVA AAVAHAFGLF VAVSVGANIS GGHVNPAVTF GAFIGGNITL
110 120 130 140 150
LRGILYWIAQ LLGSVVACLI LKFATGGLAV PAFGLSAGVG VLNAFVFEIV
160 170 180 190 200
MTFGLVYTVY ATAIDPKNGS LGTIAPIAIG FIVGANILAG GAFSGASMNP
210 220 230 240 250
AVAFGPAVVS WTWTNHWVYW AGPLVGGGIA GLIYEVFFIN TTHEQLPTTD

Y
Length:251
Mass (Da):25,620
Last modified:May 1, 1992 - v1
Checksum:iCECC6BAF42F23302
GO

Sequence cautioni

The sequence CAA38633 differs from that shown. Reason: Frameshift at position 244.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8I → V in CAA51171 (PubMed:8155880).Curated1
Sequence conflicti63V → L in CAA51171 (PubMed:8155880).Curated1
Sequence conflicti63V → L in AAM65100 (Ref. 7) Curated1
Sequence conflicti96G → W in CAA81194 (PubMed:8580968).Curated1
Sequence conflicti131P → R in CAA81194 (PubMed:8580968).Curated1
Sequence conflicti132A → P in CAA38633 (PubMed:2129561).Curated1
Sequence conflicti132A → P in CAA81194 (PubMed:8580968).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54854 mRNA Translation: CAA38633.1 Frameshift.
X63552 Genomic DNA Translation: CAA45115.1
M84344 Genomic DNA Translation: AAA32806.1
X72581 mRNA Translation: CAA51171.1
AC006922 Genomic DNA Translation: AAD31569.1
CP002685 Genomic DNA Translation: AEC09303.1
AF370172 mRNA Translation: AAK43987.1
AY059134 mRNA Translation: AAL15240.1
AY087558 mRNA Translation: AAM65100.1
Z18124 mRNA Translation: CAA79107.1
Z18771 mRNA Translation: CAA79247.1
Z26215 mRNA Translation: CAA81194.1
PIRiS13718
S22202
RefSeqiNP_181221.1, NM_129238.4
UniGeneiAt.25221
At.43252
At.67051

Genome annotation databases

EnsemblPlantsiAT2G36830.1; AT2G36830.1; AT2G36830
GeneIDi818255
GrameneiAT2G36830.1; AT2G36830.1; AT2G36830
KEGGiath:AT2G36830

Similar proteinsi

Entry informationi

Entry nameiTIP11_ARATH
AccessioniPrimary (citable) accession number: P25818
Secondary accession number(s): P21652, Q42075, Q8L5T8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1992
Last sequence update: May 1, 1992
Last modified: May 23, 2018
This is version 170 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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