Aquaporin TIP1-1 - Arabidopsis thaliana (Mouse-ear cress)

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P25818 (TIP11_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 125. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Aquaporin TIP1-1

Alternative name(s):
Aquaporin TIP
Gamma-tonoplast intrinsic protein
Short name=Gamma-TIP
Tonoplast intrinsic protein 1-1
Short name=AtTIP1;1
Tonoplast intrinsic protein, root-specific RB7

Gene names

Name:	TIP1-1
Synonyms:	AQP.1
Ordered Locus Names:	At2g36830
ORF Names:	T1J8.1

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Water channel required to facilitate the transport of water, diffusion of amino acids and/or peptides from the vacuolar compartment to the cytoplasm. Does not promote glycerol permeability. May play a role in the control of cell turgor and cell expansion. Its function is impaired by Hg²⁺. May be involved in a vesicle-based metabolite routing through or between pre-vacuolar compartments and the central vacuole. Transports urea in yeast cells in a pH-independent manner. Transports H₂O₂ in yeast cells. Ref.10 Ref.13 Ref.14 Ref.16
Subunit structure	Interacts with cucumber mosaic virus (CMV) Protein 1a. Ref.15
Subcellular location	Vacuole membrane; Multi-pass membrane protein. Note: Tonoplast. Specifically located in the tonoplast of lytic or degradative vacuoles (LV) By similarity. Ref.14
Tissue specificity	In all the vegetative organs, but not in seeds. Preferentially expressed in roots. Ref.11
Induction	By gibberellins.
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Disruption phenotype	Plants display lesion formation or plant death, and low contents of glucose, fructose, inositol, and threonic, succinic, fumaric, and malic acids. Ref.14
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. TIP (TC 1.A.8.10) subfamily. [View classification]
Sequence caution	The sequence CAA38633.1 differs from that shown. Reason: Frameshift at position 244.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane Vacuole
Domain	Repeat Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide transmembrane transport Inferred from direct assay Ref.16. Source: TAIR
Cellular component	central vacuole Inferred from direct assay. Source: TAIR chloroplast envelope Inferred from direct assay. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plant-type vacuole membrane Inferred from direct assay. Source: TAIR protein storage vacuole Inferred from direct assay. Source: TAIR
Molecular function	urea transmembrane transporter activity Inferred from genetic interaction. Source: TAIR water channel activity Inferred from direct assay Ref.10. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 251

251

Aquaporin TIP1-1

PRO_0000064008

Regions

Topological domain

1 – 23

Cytoplasmic Potential

Transmembrane

24 – 44

Helical; Name=1; Potential

Topological domain

45 – 56

Vacuolar Potential

Transmembrane

57 – 77

Helical; Name=2; Potential

Topological domain

78 – 103

Cytoplasmic Potential

Transmembrane

104 – 124

Helical; Name=3; Potential

Topological domain

125 – 143

Vacuolar Potential

Transmembrane

144 – 164

Helical; Name=4; Potential

Topological domain

165 – 172

Cytoplasmic Potential

Transmembrane

173 – 193

Helical; Name=5; Potential

Topological domain

194 – 218

Vacuolar Potential

Transmembrane

219 – 239

Helical; Name=6; Potential

Topological domain

240 – 251

Cytoplasmic Potential

Motif

85 – 87

NPA 1

Motif

199 – 201

NPA 2

Experimental info

Mutagenesis

118

C → T: Strongly reduces the mercury-sensitivity. Ref.11

Sequence conflict

I → V in CAA51171. Ref.3

Sequence conflict

V → L in CAA51171. Ref.3

Sequence conflict

V → L in AAM65100. Ref.7

Sequence conflict

G → W in CAA81194. Ref.9

Sequence conflict

131

P → R in CAA81194. Ref.9

Sequence conflict

132

A → P in CAA38633. Ref.1

Sequence conflict

132

A → P in CAA81194. Ref.9

Sequences

Sequence

Length

Mass (Da)

Tools

P25818 [UniParc].

Last modified May 1, 1992. Version 1.
Checksum: CECC6BAF42F23302
Show »

FASTA

251

25,620

        10         20         30         40         50         60 
MPIRNIAIGR PDEATRPDAL KAALAEFIST LIFVVAGSGS GMAFNKLTEN GATTPSGLVA 

        70         80         90        100        110        120 
AAVAHAFGLF VAVSVGANIS GGHVNPAVTF GAFIGGNITL LRGILYWIAQ LLGSVVACLI 

       130        140        150        160        170        180 
LKFATGGLAV PAFGLSAGVG VLNAFVFEIV MTFGLVYTVY ATAIDPKNGS LGTIAPIAIG 

       190        200        210        220        230        240 
FIVGANILAG GAFSGASMNP AVAFGPAVVS WTWTNHWVYW AGPLVGGGIA GLIYEVFFIN 

       250 
TTHEQLPTTD Y

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Root-specific genes from tobacco and Arabidopsis homologous to an evolutionarily conserved gene family of membrane channel proteins." Yamamoto Y.T., Cheng C.-L., Conkling M.A. Nucleic Acids Res. 18:7449-7449(1990) [PubMed: 2129561] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Root.
[2]	"Vegetative and seed-specific forms of tonoplast intrinsic protein in the vacuolar membrane of Arabidopsis thaliana." Hoefte H.R., Hubbard L., Reizer J., Ludevid D., Kerman E.M., Chrispeels M.J. Plant Physiol. 99:561-570(1992) [PubMed: 16668923] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Cloning of two gibberellin-regulated cDNAs from Arabidopsis thaliana by subtractive hybridization: expression of the tonoplast water channel, gamma-TIP, is increased by GA3." Phillips A.L., Huttly A.K. Plant Mol. Biol. 24:603-615(1994) [PubMed: 8155880] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Landsberg erecta. Tissue: Flower bud.
[4]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[6]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[7]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[8]	"An inventory of 1152 expressed sequence tags obtained by partial sequencing of cDNAs from Arabidopsis thaliana." Hoefte H., Desprez T., Amselem J., Chiapello H., Rouze P., Caboche M., Moisan A., Jourjon M.-F., Charpenteau J.-L., Berthomieu P., Guerrier D., Giraudat J., Quigley F., Thomas F., Yu D.-Y., Mache R., Raynal M., Cooke R. Lescure B. Plant J. 4:1051-1061(1993) [PubMed: 8281187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-116 AND 243-251. Strain: cv. Columbia. Tissue: Seedling.
[9]	"Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non-redundant ESTs." Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C., Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R., Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J. Hoefte H. Plant J. 9:101-124(1996) [PubMed: 8580968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 96-207. Strain: cv. Columbia. Tissue: Seedling.
[10]	"The vacuolar membrane protein gamma-TIP creates water specific channels in Xenopus oocytes." Maurel C., Reizer J., Schroeder J.I., Chrispeels M.J. EMBO J. 12:2241-2247(1993) [PubMed: 8508761] [Abstract] Cited for: FUNCTION.
[11]	"Characterization of a new vacuolar membrane aquaporin sensitive to mercury at a unique site." Daniels M.J., Chaumont F., Mirkov T.E., Chrispeels M.J. Plant Cell 8:587-599(1996) [PubMed: 8624437] [Abstract] Cited for: TISSUE SPECIFICITY, MUTAGENESIS OF CYS-118.
[12]	"From genome to function: the Arabidopsis aquaporins." Quigley F., Rosenberg J.M., Shachar-Hill Y., Bohnert H.J. Genome Biol. 3:RESEARCH0001.1-RESEARCH0001.17(2002) [PubMed: 11806824] [Abstract] Cited for: NOMENCLATURE.
[13]	"Urea transport by nitrogen-regulated tonoplast intrinsic proteins in Arabidopsis." Liu L.-H., Ludewig U., Gassert B., Frommer W.B., von Wiren N. Plant Physiol. 133:1220-1228(2003) [PubMed: 14576283] [Abstract] Cited for: FUNCTION.
[14]	"Loss of TIP1;1 aquaporin in Arabidopsis leads to cell and plant death." Ma S., Quist T.M., Ulanov A., Joly R., Bohnert H.J. Plant J. 40:845-859(2004) [PubMed: 15584951] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION.
[15]	"Arabidopsis tonoplast proteins TIP1 and TIP2 interact with the cucumber mosaic virus 1a replication protein." Kim M.J., Kim H.R., Paek K.-H. J. Gen. Virol. 87:3425-3431(2006) [PubMed: 17030879] [Abstract] Cited for: INTERACTION WITH CMV PROTEIN 1A.
[16]	"Specific aquaporins facilitate the diffusion of hydrogen peroxide across membranes." Bienert G.P., Moeller A.L.B., Kristiansen K.A., Schulz A., Moeller I.M., Schjoerring J.K., Jahn T.P. J. Biol. Chem. 282:1183-1192(2007) [PubMed: 17105724] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X54854 mRNA. Translation: CAA38633.1. Frameshift. X63552 Genomic DNA. Translation: CAA45115.1. M84344 Genomic DNA. Translation: AAA32806.1. X72581 mRNA. Translation: CAA51171.1. AC006922 Genomic DNA. Translation: AAD31569.1. CP002685 Genomic DNA. Translation: AEC09303.1. AF370172 mRNA. Translation: AAK43987.1. AY059134 mRNA. Translation: AAL15240.1. AY087558 mRNA. Translation: AAM65100.1. Z18124 mRNA. Translation: CAA79107.1. Z18771 mRNA. Translation: CAA79247.1. Z26215 mRNA. Translation: CAA81194.1.
IPI	IPI00531328.
PIR	S13718. S22202.
RefSeq	NP_181221.1. NM_129238.3.
UniGene	At.25221. At.43252. At.67051. At.71424.
3D structure databases
ProteinModelPortal	P25818.
SMR	P25818. Positions 13-244.
ModBase	Search...
Protein-protein interaction databases
IntAct	P25818. 1 interaction.
STRING	P25818.
Protein family/group databases
TCDB	1.A.8.10.3. major intrinsic protein (MIP) family.
Proteomic databases
PRIDE	P25818.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G36830.1; AT2G36830.1; AT2G36830.
GeneID	818255.
GenomeReviews	Gene locus AT2G36830 in contig CT485783_GR.
KEGG	ath:AT2G36830.
NMPDR	fig\|3702.1.peg.10785.
Organism-specific databases
GeneFarm	4855. 182.
TAIR	At2g36830.
Phylogenomic databases
eggNOG	KOG0223.
GeneTree	EPGT00070000027972.
HOGENOM	HBG705794.
InParanoid	P25818.
OMA	INTTHEQ.
PhylomeDB	P25818.
ProtClustDB	PLN00027.
Gene expression databases
ArrayExpress	P25818.
Genevestigator	P25818.
GermOnline	AT2G36830. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR023271. Aquaporin-like. IPR000425. MIP. IPR022357. MIP_CS. [Graphical view]
Gene3D	G3DSA:1.20.1080.10. MIP. 1 hit.
KO	K09873.
PANTHER	PTHR19139. MIP. 1 hit.
Pfam	PF00230. MIP. 1 hit. [Graphical view]
PRINTS	PR00783. MINTRINSICP.
SUPFAM	SSF81338. MIP. 1 hit.
PROSITE	PS00221. MIP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TIP11_ARATH

Accession

Primary (citable) accession number: P25818
Secondary accession number(s): P21652, Q42075, Q8L5T8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 1992
Last sequence update:	May 1, 1992
Last modified:	December 14, 2011
This is version 125 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents